Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,163,371	0	C	T	41.4%	80.7 / 30.0	59	L38F (CTC→TTC)	thiK	thiamine kinase
Reads supporting (aligned to +/- strand):  ref base C (6/28);  new base T (24/0);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 4.79e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCAGACGGGCGAAATTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGG  >  NC_000913/1163225‑1163517                                                                                                                                                     |                                                                                                                                                     ggTGCAGACGGGCGAAATTATCTGGTCAGGTAAAGGTGTCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTtctc                                                                                                                                                    <  2:773353/149‑1 (MQ=255) ggTGCAGACGGGCGAAATTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCAt                                                                                                                                                  <  2:368965/151‑1 (MQ=255) ggTGCAGACGGGCGAAATTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCAt                                                                                                                                                  <  1:602908/151‑1 (MQ=255)    gCAGACGGGCGAAATTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GGTTTTTCttt                                                                                                                                                 >  2:463807/1‑149 (MQ=255)      aGACGGGCGAAATTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTTTTAttt                                                                                                                                                >  1:673099/1‑147 (MQ=255)            gCGAAATTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAgg                                                                                                                                       <  1:952292/151‑1 (MQ=255)            gCGAAATTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAgg                                                                                                                                       <  1:887720/151‑1 (MQ=255)             cGAAATTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGG‑GTTTTTTCTTTTTACAtttggg                                                                                                                                      >  2:963647/1‑146 (MQ=255)                aaTTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGG‑GGTTTGTTCTTTAACATCAAGGcc                                                                                                                                    >  2:577212/1‑150 (MQ=255)                 aTTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAg                                                                                                                                  <  2:229587/151‑1 (MQ=255)                                 aaGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTTTCTTTTTACATCAGAGCCCG‑CGTTTttttgt                                                                                                                      >  2:414011/1‑146 (MQ=255)                                 aaGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTCCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTc                                                                                                                 <  1:798757/151‑1 (MQ=255)                                     tGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GGTTTTTTCTTTTACAACAAGGCCCG‑CGGTTTTTTTTGCGGCAg                                                                                                               >  2:733651/1‑149 (MQ=255)                                       ccGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGcc                                                                                                             <  2:148589/149‑1 (MQ=255)                                       ccGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGc                                                                                                           <  2:56992/151‑1 (MQ=255)                                             cGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGAt                                                                                                      <  2:413164/150‑1 (MQ=255)                                              gcagcaATACTCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGT‑GATTTTTCTTTGGAAATCAGGGCCAT‑CGCTtttttgggtgggcgcttcac                                                                                                         >  2:351167/1‑132 (MQ=255)                                               cagcaATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCt                                                                                                   <  1:306654/151‑1 (MQ=255)                                                  caATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTTTCATTTAAAAACAAGGCCAG‑CGCTTTTTTTTGCGTCAGCCGccccaacc                                                                                                    >  1:954654/1‑140 (MQ=255)                                                   aataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATg                                                                                               <  2:332949/151‑1 (MQ=255)                                                    ataatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATgc                                                                                              <  1:242768/151‑1 (MQ=255)                                                      aatCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATgcg                                                                                             <  2:771061/150‑1 (MQ=255)                                                          ccATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGG‑GGTTTTTTCTTTAAAAACAAGGGCAG‑CGGTTTTTTGTGGGTGAGCCGCCCGACCCCGATGcgcc                                                                                           >  1:669066/1‑148 (MQ=255)                                                             tCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGAG‑GGTTTTTCATTTCACATCCGGGCCAG‑CGCTTTGTTTGTCGGCCGGCGCCCGATCCtacggcgcc                                                                                           >  2:252907/1‑137 (MQ=255)                                                              cACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTcc                                                                                    <  1:734822/151‑1 (MQ=255)                                                                 gcgcgACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCcgcg                                                                                 <  2:963803/151‑1 (MQ=255)                                                                  cgagacgaATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGG‑GGTTTTTCAATGAAAATAAAGGACGA‑CGGTGTGGTGTGGGTTGGCCCCAAGATCCTGATgggtc                                                                                           >  2:504608/4‑136 (MQ=255)                                                                    cgacgaATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GGTTTTTTTTTTAAAATCAAGGCCAG‑CGGTTTTTTTTGTGGCAGCCCCACGAACCTGATGcgccgccgcccccggt                                                                               >  2:31779/1‑140 (MQ=255)                                                                       cgaATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTg                                                                          <  2:956741/151‑1 (MQ=255)                                                                            tGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGG‑GGTTTTTCTTTGTAAAACAGGGCCCC‑CGGTTTTTTTTTCGTCAGCCGCCCGATCCCGATGCGCCCCCGTCCGCG‑Ttttt                                                                           >  1:236844/1‑145 (MQ=255)                                                                             gCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCa                                                                     <  2:794452/150‑1 (MQ=255)                                                                                 tCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGG‑GGTTTTTCATTTTAAAACAAGGCCCG‑CGGTTTTTTGTTGGGCAGCCGCACGATCCTCTTGCGCCGCAGGCCCCG‑GTCTTTCGcccgga                                                                  >  1:249782/1‑145 (MQ=255)                                                                                    cgcTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCggg                                                             <  1:611439/151‑1 (MQ=255)                                                                                              cGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGAGGTTTTTTCTTTTAA‑ATCAAGGCCCG‑CGGTTTTTTTTTGGTCCGCCCCCCCATCCTGATGCGCCGCAGGCCCCC‑GTCTTTCGCCCGGATCGGGGttttt                                                       >  1:991694/1‑145 (MQ=255)                                                                                                 aGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGG‑GGTTTTTCCTTTTACAACAAGGGCCC‑CGGTTTTTTTTGGGGCTGGCGCCCCGTCCTGATGGGCCCCCGGCCCCG‑GTCTTGCGCCCGTATCGGGGGTTTTCACaa                                                  >  1:885670/1‑149 (MQ=255)                                                                                                  gTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTa                                               <  2:762345/151‑1 (MQ=255)                                                                                                    atcatcCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTAc                                              <  2:934305/150‑1 (MQ=255)                                                                                                     tcatcCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGAGGTTTTTTCTTTTAAAAT‑AAGGCCAG‑CGGTTTTTTGTGCGGCAGCCGCACGCTCCTGATGCGCCGCAGGCCCCG‑GTTTTTCGCCACGATCCGGCTTTTTTCCAACCAccc                                            >  2:542250/1‑151 (MQ=255)                                                                                                     tcatcCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GAGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGC‑TTATCACAACTAccc                                            >  1:996456/1‑150 (MQ=255)                                                                                                      catcCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GGTTTTTCATTTAACATCAAGGCCAG‑CGGTTTTTTTTGCGTCAGCCGCCCGATCCTGATGCGCCGCCGGCCGCG‑TTCTTTCGCCCGTATCGGGCTTTTTTACAACTAcccc                                           >  1:875953/1‑150 (MQ=255)                                                                                                         cccGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCgcat                                        <  2:929750/151‑1 (MQ=255)                                                                                                          ccGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGG‑GGTTTTTCCTTTTACATCAAGGCCCG‑CGGTTTTTTTTGGGTCAGCCGCACGAGCCTGATTCGCCGCAGGCCGCG‑TTCTTGCGCCCGGATCGGGGTTTTTCACAAACAccc                                            >  1:70455/1‑146 (MQ=255)                                                                                                          ccGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCgcatg                                       <  2:854305/151‑1 (MQ=255)                                                                                                          ccGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GGTTTTTCTTTTAAAATCAAGGCCAG‑CGGTTTTTTTTGGGTCAGCCGCACGATCCCGATGCGCCCCCGTCCCCC‑GTCTTTGGCCCGGATCGGGGTTTTTTACAAATAcccccctg                                       >  1:810373/1‑146 (MQ=255)                                                                                                           cGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGG‑GGTTTCTTATTTTACAACAAGGCCCG‑CGTTTTTTTGTGGGTCAGCCCCACGAACCCGCTGCGCCCCAGGCCCCG‑TTCTTTCGCCAGTATCGGGCTTTTTTACAACAACCCccatg                                       >  1:321330/1‑150 (MQ=255)                                                                                                              cACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCgcatgcat                                    <  2:183900/150‑1 (MQ=255)                                                                                                               acgacgTTTAATAGTGGGCTTAGTGGC‑GGGA‑GGTTTTTCATTTAACATCAGCGGCAGACGGTTT‑TTTTGGGTCAGCCCCGCGAACCTGGTACGGCGCCGGCCGCC‑GTTTTTTGCCAGCATCGTGGTTTTTCCCCACAAcccccctcac                                     >  1:33929/1‑141 (MQ=255)                                                                                                                cgacgTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTTTCATTTAAAAACAGGGCCCG‑CGTTTTGTTTTGGGTCAGCCGCCCGATCCTGGTGCGCCGCAGTCCGCG‑TTCTTTCGCCAGTATCGGGCTTTTTTACAACAACCCCCATGCAtt                                   >  1:420946/1‑149 (MQ=255)                                                                                                                 gacgTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCa                                <  1:885310/151‑1 (MQ=255)                                                                                                                  acgTTTAATAGTGGGCTTAGTGGCGGGGG‑GTTTTTTCTTTGAAAAT‑AGGGCCCG‑CGGTTTTTTGGGGGGCACCCGCCCCATCCCGATGCCCCGCCGTCCGCGCTTCTT‑CGCCACGATCGGGGTTTATCACCAATCACCCCATGCAtttta                                >  1:882508/1‑147 (MQ=255)                                                                                                                    gTTTAATAGTGGGCTTAGTGGC‑GGGG‑GGTTTTTCTTTTAAAATCAGGGGCCG‑CGGTTTTTTTTGGGGCAGCCCCACGATCCCGATGCGCCCCCGGCCCCG‑GTCTTTCGCCCGCATCGGGGTTTTTTACAAATAACCCCACGCATTTCAcc                              >  1:63539/1‑150 (MQ=255)                                                                                                                        aaTAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAg                          <  1:409622/150‑1 (MQ=255)                                                                                                                         aTAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGcc                        <  2:989200/151‑1 (MQ=255)                                                                                                                            gTGGGCTTAGTGGC‑GGGG‑GGTTTTTCATTTAAAATCAAGGGCAG‑CGGTTTGTTGTGGGTCCGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTTCGCCCGTATCGGGGTTTTTCACAACTACCCCCATGCATTTCACCCCAGCCGc                      >  1:848241/1‑150 (MQ=255)                                                                                                                                        gC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCg         <  1:535856/151‑1 (MQ=255)                                                                                                                                             gA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGAc     >  1:787360/1‑151 (MQ=255)                                                                                                                                              a‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACt    <  1:494268/151‑1 (MQ=255)                                                                                                                                                gTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTg   >  1:906508/1‑151 (MQ=255)                                                                                                                                                gTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTg   >  1:214180/1‑151 (MQ=255)                                                                                                                                                 ttttCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTgg  >  1:34802/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GGTGCAGACGGGCGAAATTATCTGGTCAGGTAAAGGTGCCGTTTCGCAGCAATAATCCCATCACGCGCGACGAATTGCTGTCGCGCTTTTTCCCGCAGTATCATCCCGTCACGACGTTTAATAGTGGGCTTAGTGGC‑GGGA‑GTTTTCTCATTGAACATCAGGGCCAG‑CGTTTTGTTGTGCGTCAGCCGCACGATCCTGATGCGCCGCAGTCCGCG‑TTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCATTTATATCTCCGTGACTGG  >  NC_000913/1163225‑1163517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑