Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,635,114 A→C L66R (CTA→CGA)  rob ← right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,635,1140AC96.9% 195.9 / ‑5.8 64L66R (CTA→CGA) robright oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (42/20);  minor base G (0/2);  total (42/22)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCC  >  NC_000913/4634980‑4635257
                                                                                                                                      |                                                                                                                                               
cgcTCCATTCAGGAGAAGGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGAttt                                                                                                                                 <  2:540956/151‑1 (MQ=255)
 gcTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGAttt                                                                                                                                 >  2:739295/1‑150 (MQ=255)
   tCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGGATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTc                                                                                                                                >  2:592163/1‑149 (MQ=255)
    ccATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGAc                                                                                                                             <  2:666717/151‑1 (MQ=255)
    ccATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGAc                                                                                                                             <  1:398387/151‑1 (MQ=255)
    ccATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTAAGGCGTCGTGCGACCGCCGATTTCGAc                                                                                                                             <  2:172763/151‑1 (MQ=255)
     cATTCAGGAGAACGGCGGTAAAGTGCAGGGGTCGGGGCAAACTGCTTTTTGAATCCGCGGGAAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGGCGCGCAGTCGGGCGTCGTGCGACCGCCGATTTCGAc                                                                                                                             <  2:688441/150‑1 (MQ=255)
     cATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGGAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATTGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCg                                                                                                                               >  2:360897/1‑148 (MQ=255)
      aTTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACa                                                                                                                            >  1:485707/1‑150 (MQ=255)
         cAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGTTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAac                                                                                                                         <  2:19396/150‑1 (MQ=255)
             agaACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAacgacg                                                                                                                     >  1:796051/1‑150 (MQ=255)
              gtaCGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTAAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATGGCAGCGCGATGTCCAGAACCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAAcgacga                                                                                                                    <  2:764493/148‑1 (MQ=255)
              gaACGGCGGTAAAGTGCAGGAGTCGGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAGGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAAcgacga                                                                                                                    <  2:313245/150‑1 (MQ=255)
                  ggcggTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACg                                                                                                               >  1:322403/1‑151 (MQ=255)
                     ggTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAat                                                                                                            >  1:310863/1‑151 (MQ=255)
                     ggTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAat                                                                                                            >  2:701194/1‑151 (MQ=255)
                     ggTAAAGTGCAGGAGTCGGGGCAAACGGTTTCTTAAATGCGGGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCGGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGaa                                                                                                             <  2:857874/150‑1 (MQ=255)
                        acaGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAatat                                                                                                          >  2:550589/3‑150 (MQ=255)
                        aaaGTGCGGGAGTCTGGGCAAACTGCTTCTTAAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCGGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAatat                                                                                                          <  1:841773/150‑1 (MQ=255)
                        aaaGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAatat                                                                                                          <  1:613540/150‑1 (MQ=255)
                         aaGTGCAGGAGTCGGGGCAACCTGCTTCTTAAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATAc                                                                                                        <  2:847447/151‑1 (MQ=255)
                          aGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATAcg                                                                                                       >  1:273423/1‑151 (MQ=255)
                               aGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGcc                                                                                                   >  2:277163/1‑150 (MQ=255)
                                    tCTGGGAAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATa                                                                                               <  1:222560/149‑1 (MQ=255)
                                       gggCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATg                                                                                          >  2:913029/1‑151 (MQ=255)
                                          cAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGcc                                                                                       >  1:417806/1‑151 (MQ=255)
                                              cTGCTTCTTGAATGCGCGGGAAAATGTCTGTGGAGAGTGGAAGCGGTATTGCAGCGGGATGTCAAGAATGGGACGGCCTGTCGGGCTGCGGGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGt                                                                                    <  2:233832/150‑1 (MQ=255)
                                               tGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTg                                                                                   >  1:74371/1‑150 (MQ=255)
                                                  ttcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACAt                                                                               >  2:18670/1‑151 (MQ=255)
                                                  ttcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACAt                                                                               >  2:779900/1‑151 (MQ=255)
                                                  ttcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGCCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGt                                                                                    <  2:877233/146‑1 (MQ=255)
                                                   tcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACAt                                                                               <  1:768721/150‑1 (MQ=255)
                                                      tGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCttt                                                                           >  2:638573/1‑151 (MQ=255)
                                                        aaTGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTa                                                                          >  1:418857/1‑150 (MQ=255)
                                                         aTGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTaa                                                                         >  1:264551/1‑150 (MQ=255)
                                                         aTGCGCGGGAAAATGTCTGTGGAGAGTCGAAGCGGTATGGCAGCGCGATGCCAAGAATCGGCCGCGCAGTCAGGCGTCGGGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTaa                                                                         <  2:233186/150‑1 (MQ=255)
                                                             gcgGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACAtt                                                                    >  1:61953/1‑151 (MQ=255)
                                                             gcgGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACAtt                                                                    >  2:824485/1‑151 (MQ=255)
                                                              cgGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATtc                                                                   >  2:736254/1‑151 (MQ=255)
                                                               gggTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATtct                                                                  >  1:772349/1‑151 (MQ=255)
                                                                ggTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATtctc                                                                 >  2:313397/1‑151 (MQ=255)
                                                                  tAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTg                                                               >  2:704070/1‑151 (MQ=255)
                                                                        tCTGTTGAGAGTCGATGCGGTATTGCAGCGCGATGTCCAGAATCGGAAGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAAAATTCTCTGTAAGTg                                                         >  2:1236/1‑151 (MQ=255)
                                                                          tGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGcc                                                       >  1:558686/1‑151 (MQ=255)
                                                                           gTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAg                                                           <  2:942773/146‑1 (MQ=255)
                                                                            ttGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCa                                                      <  1:572033/150‑1 (MQ=255)
                                                                            ttGAAAGTCGAAGCGGTATTGCGGCGCAATTCCCAGAATCGGACCCGCAGTCAGGCGTGGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTg                                                         <  1:385454/147‑1 (MQ=255)
                                                                             tGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACt                                                    >  1:324569/1‑151 (MQ=255)
                                                                                 agTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTgg                                                 >  2:708381/1‑150 (MQ=255)
                                                                                     gAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATa                                             >  1:16010/1‑150 (MQ=255)
                                                                                     gAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATa                                             >  2:516213/1‑150 (MQ=255)
                                                                                     gAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATa                                             >  1:642943/1‑150 (MQ=255)
                                                                                       aGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAAcc                                          >  2:827376/1‑151 (MQ=255)
                                                                                            tataGCAGCGTGATGACCAGCATCCGACGCGGCGTCAGGCCTCGAGTGACCGCCGATTTCGACAAACGACGCGGACGGCTATACCCGCTAATAGCTTGGTCAGTGACATGTTTAATTATTCTCTGCAAGCGCCACTTGGGATAACCTGCtt                                     >  2:87816/1‑151 (MQ=255)
                                                                                               tGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTcg                                  >  1:227372/1‑151 (MQ=255)
                                                                                                    cgcgATGTCCAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCt                             >  2:218693/1‑151 (MQ=255)
                                                                                                          gTCCAGAATGGGCCGCGCAGTCGGACGTGGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACAtt                        <  1:986016/150‑1 (MQ=255)
                                                                                                             cAGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                    >  2:696405/1‑151 (MQ=255)
                                                                                                              aGAATCGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                    >  2:682082/1‑150 (MQ=255)
                                                                                                                   cGGACGCGCAGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGAc              >  1:686204/1‑151 (MQ=255)
                                                                                                                            aGTCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTg      >  1:786553/1‑150 (MQ=255)
                                                                                                                             gTCGGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGAt    <  2:661752/151‑1 (MQ=255)
                                                                                                                              tCAGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATc   >  1:673801/1‑151 (MQ=255)
                                                                                                                                aGGCGTCGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATcc  >  1:383201/1‑150 (MQ=255)
                                                                                                                                      |                                                                                                                                               
CGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCC  >  NC_000913/4634980‑4635257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑