Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,486,580	0	A	G	43.9%	83.2 / 15.9	57	G121G (GGA→GGG)	lptF	lipopolysaccharide export ABC permease
Reads supporting (aligned to +/- strand):  ref base A (7/25);  new base G (25/0);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 3.54e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGACGCTGGGCAAACTGTATACCGAAAGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAT  >  NC_000913/4486437‑4486730                                                                                                                                                 |                                                                                                                                                          atgaCGCTGGGCAAACTGTATACCGAAAGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTgg                                                                                                                                                     <  1:719016/150‑1 (MQ=255)      gCTGGGCAAACTGTATACCGAAAGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGGGGGGCCGGGGGCAt                                                                                                                                                 >  1:204584/1‑149 (MQ=255)               aCTGTATACCGAAAGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTTAAGTGTTGT‑GGGGGGGGCCGt                                                                                                                                                       >  1:274328/1‑134 (MQ=255)                 tGTATACCGAAAGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTTACGGGGTGT‑GGGGGGGGGCGCGGGGATCGCGTCGTCAgg                                                                                                                                     >  2:158413/1‑150 (MQ=255)                 tGTATACCGAAAGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGGTAACGGGGTGT‑GGGGGGGGCCGTGGGCATCGCGGCATCCgg                                                                                                                                     >  2:783019/1‑150 (MQ=255)                    ataCCGAAAGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGGGGGGCCGCGGGCATCGCGGCCTCAgg                                                                                                                                     >  1:461976/1‑147 (MQ=255)                         gAAAGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAgtgt                                                                                                                            <  1:756319/151‑1 (MQ=255)                           aaGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGGGATGT‑GGGGGGGGCCGGGGGCCTCGCGGCAACAGGGTGGAggggt                                                                                                                           >  2:312981/1‑146 (MQ=255)                                aaaTTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGa                                                                                                                     <  2:851042/151‑1 (MQ=255)                                 aaTTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTTACGTGATGT‑GGGCGGGGCCGGGGGCATCGCGTCCTCAGGGGGAAggg                                                                                                                             >  1:319291/1‑140 (MQ=255)                                  aTTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGaag                                                                                                                   <  1:427071/151‑1 (MQ=255)                                    tACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGGCCGTGGGGATCGCGTCATCAGGGTGAAGGGTTAGCAGaa                                                                                                                    >  1:863338/1‑148 (MQ=255)                                    tACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGaagc                                                                                                                  <  1:13142/150‑1 (MQ=255)                                     aCGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAAAGGGATGT‑GGGGGGGGCCGGGGGCCTCGCGGCATCAGGGAGAAGTGGTTGCAGaa                                                                                                                    >  1:919848/1‑147 (MQ=255)                                      cGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGaagcg‑aa                                                                                                              <  2:1007629/151‑1 (MQ=255)                                      aggTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGGTAACGTGATGT‑GGGCGGGGGCGGGGGGATCGCGTCATCAGGGTGGAGTGTTAGCAGaagag                                                                                                                 >  2:955659/2‑147 (MQ=255)                                       ggTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGaagcg‑aaa                                                                                                             <  2:653051/151‑1 (MQ=255)                                                 gcCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCt                                                                                                    <  2:927471/150‑1 (MQ=255)                                                  ccTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAAAGGGATGG‑GGGCGGGGCCGGGGGCATTGCGGCCTCAAGGAGAAGGGTTAGAAGAAGCG‑AAAGAGAAACCttg                                                                                                  >  2:406288/1‑149 (MQ=255)                                                   cTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAAAGGGATGT‑GGGGGGGGGCGGGGGCATCGCGGCCTCAGGATGAAGGGGTTGGAGAAGCG‑AAAACGGAcccccg                                                                                                  >  2:975254/1‑147 (MQ=255)                                                     gCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGc                                                                                                 <  2:565173/149‑1 (MQ=255)                                                     gCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGc                                                                                                 <  1:89286/149‑1 (MQ=255)                                                     gCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCAt                                                                                               <  1:499018/151‑1 (MQ=255)                                                       ggCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGGGGGGCCGTGGGCATCGCGTCCTCAGGGTGAAGTGTTAGCAGAAGCG‑AAAGAGAAACCCGGCATgg                                                                                             >  1:407875/1‑151 (MQ=255)                                                       ggCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATgg                                                                                             <  2:595851/151‑1 (MQ=255)                                                       ggCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATgg                                                                                             <  1:451643/151‑1 (MQ=255)                                                           tGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGGTGT‑GGGCGGGGCCGTGGGCATAGCGGCATCAGGATGGAGTGGTTGCAGAAGCG‑GAAGCGAACCCCGGccaggggg                                                                                          >  1:188504/1‑143 (MQ=255)                                                               cAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGGTAAAGTGGTGT‑GGGGGGGACCGGGGTCATTGGGGCATCATGGTTTAGGGTTTGCGGAAGAGAAAAGGGAAccc                                                                                                     >  2:186735/1‑136 (MQ=255)                                                                aaaGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGGTAAAGGGATGT‑GGGGGGGGCCGCGGGCATTGCGGCCTCAGGGGGAAGAGTTTGTAGGAGCG‑GAAAAGAACCCCGGCACGGCGGGGCCgg                                                                                    >  2:216200/1‑151 (MQ=255)                                                                 aaGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGgc                                                                                   <  2:1011158/151‑1 (MQ=255)                                                                    cGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAAAGTGATGT‑GGGCGGGGCCGGGGGCATCGCGTCATCAGGGTGGAGGGGTAGCAGAAGCG‑AAAGCGAAACCCGGCATGGCGGGGCCgg                                                                                    >  1:716861/1‑147 (MQ=255)                                                                        tCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTTAAGTGATGT‑GGGGGGGGGCGGGGGCATCGCGTCCTCAGGGTGAAGTGTTAGCAGAAAAG‑AAAGCGAAACCCGGCATGGGGGGGGTGGCG‑GCA‑ggg                                                                          >  2:570796/1‑151 (MQ=255)                                                                           ggTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑ggg                                                                          <  1:514673/148‑1 (MQ=255)                                                                                aaGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCa                                                                  <  1:768760/151‑1 (MQ=255)                                                                                    ggCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAAAGTGGTGT‑GGGGGGGGCCCGGGGGATCGGGGGATAAGGGTGAAGGGGTTGGAGAAGGG‑AAAGGGAACCCCGGCATGGGGGGGGTGGGG‑GAA‑gggggaattc                                                                   >  2:3840/1‑139 (MQ=255)                                                                                     gCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAAAGAGATGTGGGGGGGGACCG‑GGGCATCGCGGCATCAGGATGAAGGGTTAGCAGAAGCG‑AAAGCGAACCCCGGCATGGGGGGGCCGGGG‑CCA‑GGGGAAATTCAGCaa                                                              >  2:741435/1‑150 (MQ=255)                                                                                          gATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAAAGTGATGT‑GGGGGGGGGCGGGGGGATCGCGTCATTAGGATGGAGGGTTAGCAGAAGCG‑AAAAAGAAACCCGGCATGGCGGCGCCGGGG‑CCA‑GGGGCAATCCAGCaa                                                              >  1:730254/1‑145 (MQ=255)                                                                                            tCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTTTAACGATGG‑GGGCGGGGGCGGGGGGATCGCGGCCACAGGGTGAAGCTTTAACCAAAAGG‑AAAGGGAAACCCGGGATGGGGGCGCTGGCGCCAA‑gggaa                                                                        >  1:716554/1‑132 (MQ=255)                                                                                              cTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAAAATGATGT‑GGGGGGGGACCTGGGGATCGCGGCATACGGGAGAAGGGGTAAGAGAAGGG‑AAAACGAAACCCGGCACGGGGGGGCTGGCG‑CAAGGGGAAATTCCacaaaccaccaa                                                       >  1:717839/1‑138 (MQ=255)                                                                                               ttGCGGTATTCACGGCAATCGTAGCGGCGGTTAAAGTGATGT‑GGGGGGGGCCGGGGGCATCGCGGCATCAGGATGGAGTGTTTGCAGAAGCG‑GAAACGAACCCCGGGATGGCGGGGCTGGGG‑CAA‑GGGGAAATCCAGCAAGCGACTaa                                                      >  1:817666/1‑148 (MQ=255)                                                                                                 gCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCa                                                 <  1:335902/151‑1 (MQ=255)                                                                                                 gCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCa                                                 <  2:848494/151‑1 (MQ=255)                                                                                                   ggTATTCACGGCAATCGTAGCGGCGGTTTACGTGGTGT‑GGGGGGGGCCGTGGGCATCGCGTCATCAGGGTGGAGGGGTAGGAGAAGCG‑AAAACGAACCCCGGCCAGGGGGGGCGGGGG‑CCA‑GGGGAATTTCAGCaaaacaacaa                                                      >  2:543001/1‑136 (MQ=255)                                                                                                   ggTATTCACGGCAATCGTAGCGGCGGTTTACGTGGTGT‑GGGGGGGGCCGGGGTCATCGCGGCCTCCGGAGGAAGGGTTAGGAGAAGCG‑AAAACGAAACCCCGCCTGGGGGCGCCGGGG‑CGA‑GGGGAAATTCACCAAACGACCAAAGGGAg                                                >  2:488247/1‑150 (MQ=255)                                                                                                     tATTCACGGCAATCGTAGCGGCGGTTAACGGGATTT‑GGGCGGGGCCGTGGTCATCGCGTCCTCAGGGTGAAGGGTTAGCAGAAACG‑AAAACGAAACCCGGCCTGGCGGCGCCGGGG‑CCA‑GGGGAATTTCAGCAAACGACTAATGGGAGCTc                                             >  1:389079/1‑151 (MQ=255)                                                                                                             gCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGt                                     <  2:819138/151‑1 (MQ=255)                                                                                                                   gTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCg                               <  2:1010392/151‑1 (MQ=255)                                                                                                                       cggcggTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGGGCTGTTCATCGaa                             >  2:643284/1‑149 (MQ=255)                                                                                                                         gcggTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCg                         >  2:797981/1‑151 (MQ=255)                                                                                                                          cggTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGt                        <  2:441470/151‑1 (MQ=255)                                                                                                                           ggTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGtt                       <  2:596436/151‑1 (MQ=255)                                                                                                                           ggTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGtt                       <  2:238117/151‑1 (MQ=255)                                                                                                                            gTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTg                      <  2:653836/151‑1 (MQ=255)                                                                                                                               aaCGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACg                   <  2:82453/151‑1 (MQ=255)                                                                                                                                  gTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGc                 >  1:686781/1‑150 (MQ=255)                                                                                                                                            gCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTc        >  2:975727/1‑150 (MQ=255)                                                                                                                                               ggACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCAATTTCaa      >  2:775413/1‑149 (MQ=255)                                                                                                                                                 aCCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAt  <  2:274312/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                          ATGACGCTGGGCAAACTGTATACCGAAAGTGAAATTACGGTAATGCATGCCTGCGGCCTGAGCAAAGCGGTTCTGGTGAAAGCGGCAATGATCCTTGCGGTATTCACGGCAATCGTAGCGGCGGTTAACGTGATGT‑GGGCGGGACCGTGGTCATCGCGTCATCAGGATGAAGTGTTAGCAGAAGCG‑AAAGCGAACCCTGGCATGGCGGCGCTGGCG‑CAA‑GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAT  >  NC_000913/4486437‑4486730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑