Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,538,109	0	A	C	42.7%	128.4 / 22.5	76	G219G (GGT→GGG)	nanM	N‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base A (36/7);  new base C (0/32);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.93e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTT  >  NC_000913/4537961‑4538258                                                                                                                                                        |                                                                                                                                                      tCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCAcc                                                                                                                                                     >  2:942715/1‑151 (MQ=255)   cccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCtt                                                                                                                                                   <  2:253772/151‑1 (MQ=255)   cccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCtt                                                                                                                                                   <  2:672655/151‑1 (MQ=255)      gCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCttt                                                                                                                                                  <  2:821054/149‑1 (MQ=255)       cAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTAt                                                                                                                                                <  2:581671/150‑1 (MQ=255)         aaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTc                                                                                                                                              >  1:862197/1‑150 (MQ=255)          aaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTcac                                                                                                                                            <  2:545361/151‑1 (MQ=255)             cGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAAc                                                                                                                                         >  1:229190/1‑151 (MQ=255)              gCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAAcc                                                                                                                                        >  1:655650/1‑151 (MQ=255)                cAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAAccgc                                                                                                                                      >  1:106700/1‑151 (MQ=255)                    tACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACcgccgc                                                                                                                                   >  1:548091/1‑150 (MQ=255)                     aCGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCAc                                                                                                                                 >  2:889801/1‑151 (MQ=255)                      cGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTAAAATTATTCCCGGTAAAATAAATTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGTTTTT‑ATCCCCTTTATTCACAACCGCCGCAcc                                                                                                                                <  1:284950/151‑1 (MQ=255)                        ccATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCAcc                                                                                                                                >  1:405746/1‑149 (MQ=255)                            cTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGt                                                                                                                          >  1:346835/1‑151 (MQ=255)                             tGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGtt                                                                                                                         >  1:235208/1‑151 (MQ=255)                                      gaCGGGAGCAAGTTTATTCCATTTAAAATTATTCCCGGTAAAATCAATTTAAAATACGGCATCCGTTCGCAACCCTGGTTTGG‑TTT‑CGCCATTAATAAGCCAGGTTTT‑ACCCCTTTTATTCACAACCGCCGCACCAGCCGTTCCGTa                                                                                                                    <  1:474794/147‑1 (MQ=255)                                       aCGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAgg                                                                                                               >  2:420153/1‑151 (MQ=255)                                               aaccTTTTTCCTTTTAAAATTTTTCCCGGTAAAACAAAGTCAAATTCCGCCACCCGTTCCCAATCC‑GGTTTGGCCTC‑CGCCTTTAAAAAGCCAGTTTTT‑ACCCCTTTTTTCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCg                                                                                                       <  2:649664/148‑1 (MQ=255)                                                aGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                      >  1:431108/1‑151 (MQ=255)                                                  cTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                     >  2:413335/1‑150 (MQ=255)                                                      ttCCATTTAAAATTATTACCGGTAAAATCAAGTTCAAATACGGCTCCCTTTCGCAATCCTGGTTTGG‑CTT‑CCCCATAAATAACCCAGGTTTT‑ACCCCCTTTATTCCCCCCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCg                                                                                                 <  2:678605/150‑1 (MQ=255)                                                       tCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGt                                                                                                >  2:584278/1‑150 (MQ=255)                                                         cATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAAc                                                                                             >  2:335342/1‑151 (MQ=255)                                                          aTTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACt                                                                                            >  1:895194/1‑151 (MQ=255)                                                               aaaTTATTACCGGTAAAATCAATTTCAAATACGGCACCCTTTCGCAATCCTGTTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACCCCAtt                                                                                       <  1:521024/151‑1 (MQ=255)                                                                aaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTg                                                                                      >  1:906314/1‑151 (MQ=255)                                                                aaTTATTACCGGTAAAATCAATTTAAAATCCGGCTCCCGTTCGCAATCCTGTTTTGG‑CTT‑CGCCATTAATAGGCCGGTTTTT‑ATCCCCTTTATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc                                                                                          <  2:762450/147‑1 (MQ=255)                                                                aaTTATACCCGGTCAAATCAAGTTGAAATTCGCCTTCCGTTCAGTGCTGTGTTTTGG‑CTT‑CGCAATTATTAATCCGGTTTTT‑ATCATTTTTATTCACAGCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCa                                                                                         <  1:874229/148‑1 (MQ=255)                                                                  ttattaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCt                                                                                    >  1:200099/1‑151 (MQ=255)                                                                   tattaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtg                                                                                   >  1:476985/1‑151 (MQ=255)                                                                        ccGGTAAAATAAAGTTAAAATACGGCACCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt                                                                                <  1:940831/149‑1 (MQ=255)                                                                        ccGGGGAAATCAAGTTCAAATACGGCACCCGTTCGCAATCCGGGTTTGG‑TTC‑CGCCATTAATAAGCCAGGTTTT‑TTCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt                                                                                  <  1:669041/147‑1 (MQ=255)                                                                         cGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTg                                                                              >  2:27464/1‑150 (MQ=255)                                                                          ggTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTg                                                                              >  2:825964/1‑149 (MQ=255)                                                                              aaataaatTTAAAATCCGGCCCCCTTCCGCAATCCTGTTTGGG‑CTC‑CGCCATTAATAACCCGGTTTTT‑ACCCCCTTTATCCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAggg                                                                          <  2:347239/149‑1 (MQ=255)                                                                              aaaTAAAGTTAAAATCCGCCACCCTTCCCAAATCTTGGTTTGC‑TTT‑CCCCATAAATACCCCGGGTTTT‑ATCCCTTTTATCCCAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAgg                                                                           <  2:636308/148‑1 (MQ=255)                                                                               aaTCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc                                                                       >  1:311231/1‑151 (MQ=255)                                                                                aTCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc                                                                       >  2:606125/1‑150 (MQ=255)                                                                                  cAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa                                                                     >  1:740996/1‑150 (MQ=255)                                                                                  cAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACC‑CCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaa                                                                    >  2:53506/1‑150 (MQ=255)                                                                                   aaGTTAAAATCCGGCACCCGTTCGCAACCCT‑GTTTGG‑CTTCCCCCATTAATAAGCGAGGTTTT‑ACCCCTTTTATCCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaaa                                                                   <  2:161144/151‑1 (MQ=255)                                                                                   aaGTTAAAATACGGCACCGGTTCGCACTCCTGTTTTGG‑CTC‑CGCCATAAAT‑AGCCCGGTTTTAATCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaaa                                                                   <  1:11745/151‑1 (MQ=255)                                                                                      ttCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGac                                                                >  1:930365/1‑151 (MQ=255)                                                                                         aaaTACGGCATCCGTTCGCAATCCTGGTTTGG‑CTC‑CGCCATTAATAAGCCAGTTTTT‑ATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGacaac                                                             <  1:897923/151‑1 (MQ=255)                                                                                               ggCACCCGTCCGCAATCCTGTTTTGG‑CTC‑CCCCATTAATAAGCCAGGTTTT‑ATCCCCTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaaa                                                        <  1:847814/150‑1 (MQ=255)                                                                                               gccATCCTTTCCCAATCCTGGTTTGC‑TTT‑CGCCATTAATAAGCCAGTTTTT‑ATCCCCTTTATTCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaaa                                                        <  1:200508/148‑1 (MQ=255)                                                                                                  acccttcccaattCCTGGTTTGC‑CTC‑CGCCATAAATAACCCTGTTTTT‑ATCCCTTTTTTCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                     <  2:994758/138‑1 (MQ=255)                                                                                                    cctttCGCAATCTTGTTTTGC‑CTC‑CCCCATAAAAAAGCCAGTTTTT‑ACCCCTTTTATCCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                     <  2:919879/145‑1 (MQ=255)                                                                                                      gTTCGCAATCCTGTTTTGC‑CTC‑CCCCTTTAATACGCCGGTTTTT‑ACCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                                 <  1:914054/150‑1 (MQ=255)                                                                                                       ttCGCAATCC‑GGTTTGG‑CTTCCGCCATTAATAAGCCGGGTTTA‑ACCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                                 <  1:278704/149‑1 (MQ=255)                                                                                                        ttctcaccccggTTTTG‑CTTCCGCCATAAAAAAGCCAGGTTTT‑AACGCTTTTTTTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                    <  2:871046/136‑1 (MQ=255)                                                                                                         cgcaccccttttttGC‑CTC‑CCCCTTTAATAAGCCGTTTTTT‑ATCCCTTTTTTCCAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaa                                               <  2:655596/138‑1 (MQ=255)                                                                                                         cGCAACCTTGTTTTGT‑CTT‑CCCCATAAATAACCAATGTTTT‑ACCCCTTTTTTCCAAATCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                     <  2:915199/143‑1 (MQ=255)                                                                                                          cccaaccTTGTTTTG‑CTTCCGCCATAAAAAGGCAAGTTTTT‑ACCCCCTTTATCCAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaaga                                             <  2:607697/146‑1 (MQ=255)                                                                                                           cAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaagaa                                            >  2:514828/1‑150 (MQ=255)                                                                                                            aaTCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaat                                          >  1:411772/1‑151 (MQ=255)                                                                                                               ccTGTTTTGC‑CTC‑CCCCATAAATAACCCAGTTTTT‑ATCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaata                                         <  1:965070/149‑1 (MQ=255)                                                                                                               ccTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaataat                                       >  2:354274/1‑151 (MQ=255)                                                                                                               ccTGGTTTGG‑CTC‑CCCCATTAAAAAGCCAGTTTTT‑ATCCCTTTTATTCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaat                                          <  1:927608/148‑1 (MQ=255)                                                                                                                    ttttG‑CTTCCGCCATTATAAACCCAGTTTTT‑ATCCCCTTTATCCAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtctt                                    <  2:511410/150‑1 (MQ=255)                                                                                                                           tt‑CCCCATTAAAAAGCCGGTTTTT‑ACCCCCTTTATCCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtt                              <  1:713973/149‑1 (MQ=255)                                                                                                                                 cATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                       >  1:601449/1‑151 (MQ=255)                                                                                                                                  aTTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                       >  2:319470/1‑150 (MQ=255)                                                                                                                                   taaaaaggCCAGTTTTT‑ATCCCCTTTATACACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                       <  1:459305/142‑1 (MQ=255)                                                                                                                                     aataaGCCATGTTTT‑ATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                       <  2:361762/147‑1 (MQ=255)                                                                                                                                      ataaGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAAt                  >  1:532710/1‑151 (MQ=255)                                                                                                                                           cgagttttt‑GTCCCCTTTATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATat                <  2:107225/143‑2 (MQ=255)                                                                                                                                           ccAGTTTTT‑ATCCCCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGt               <  1:101027/149‑1 (MQ=255)                                                                                                                                            cAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTg              >  1:539330/1‑149 (MQ=255)                                                                                                                                             ggttttt‑ATCCCCTTTATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGc           <  2:134667/148‑1 (MQ=255)                                                                                                                                              gttttt‑ATCCCTTTTATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCa          <  1:410658/149‑1 (MQ=255)                                                                                                                                              ggTTTT‑CTCACCTTTTTACACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTg              <  2:30984/147‑1 (MQ=255)                                                                                                                                                tttt‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAtt        >  1:552688/1‑151 (MQ=255)                                                                                                                                                      tCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAttt   >  1:841004/1‑151 (MQ=255)                                                                                                                                                       cACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAtttt  >  1:516565/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                      TCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGG‑CTT‑CGCCATTAATAAGCCAGGTTTT‑ATCACCTTTATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTT  >  NC_000913/4537961‑4538258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑