Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,674,448 C→A P179Q (CCG→CAG)  tqsA → pheromone AI‑2 transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,4480CA98.6% 290.2 / NA 71P179Q (CCG→CAG) tqsApheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (34/36);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATT  >  NC_000913/1674302‑1674595
                                                                                                                                                  |                                                                                                                                                   
gAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGtt                                                                                                                                                 >  1:657137/1‑151 (MQ=255)
 aaCGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTg                                                                                                                                                >  2:496780/1‑151 (MQ=255)
 aaCGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTg                                                                                                                                                >  2:253643/1‑151 (MQ=255)
   cgcgGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGaa                                                                                                                                              <  1:61376/151‑1 (MQ=255)
    gcggcgATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAg                                                                                                                                             <  1:632151/151‑1 (MQ=255)
           tGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATggc                                                                                                                                      >  2:826331/1‑151 (MQ=255)
            gACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATggcg                                                                                                                                     <  1:433195/151‑1 (MQ=255)
            gACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATggc                                                                                                                                      <  2:138713/150‑1 (MQ=255)
              cGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATggcggc                                                                                                                                   <  1:199038/151‑1 (MQ=255)
               gTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGgcggcg                                                                                                                                  <  2:382620/151‑1 (MQ=255)
               gTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGgcggcg                                                                                                                                  >  2:589635/1‑151 (MQ=255)
                       ccAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAAc                                                                                                                           <  1:24821/150‑1 (MQ=255)
                       ccAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACg                                                                                                                          >  1:637766/1‑151 (MQ=255)
                                tGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTg                                                                                                                  >  2:838340/1‑150 (MQ=255)
                                  aCGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGAc                                                                                                                >  2:965367/1‑150 (MQ=255)
                                   cGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGAc                                                                                                                >  1:197037/1‑149 (MQ=255)
                                    gCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAgt                                                                                                             >  2:792096/1‑151 (MQ=255)
                                     cAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAgtg                                                                                                            <  2:375141/151‑1 (MQ=255)
                                      aGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAgtgt                                                                                                           >  2:776405/1‑151 (MQ=255)
                                        ttATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGttt                                                                                                         >  2:27840/1‑151 (MQ=255)
                                        ttATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGttt                                                                                                         >  1:167268/1‑151 (MQ=255)
                                         tATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTtc                                                                                                        >  1:291025/1‑151 (MQ=255)
                                              aaTGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTtct                                                                                                       <  2:416005/147‑1 (MQ=255)
                                              aaTGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTtct                                                                                                       <  2:249388/147‑1 (MQ=255)
                                              aaTGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCAtt                                                                                                   >  2:111982/1‑151 (MQ=255)
                                              aaTGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCAtt                                                                                                   <  1:1009617/151‑1 (MQ=255)
                                               aTGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTa                                                                                                  <  1:750155/151‑1 (MQ=255)
                                               aTGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTa                                                                                                  <  2:171914/151‑1 (MQ=255)
                                                 gCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATc                                                                                                >  1:263576/1‑151 (MQ=255)
                                                  ccATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCt                                                                                               <  1:240763/151‑1 (MQ=255)
                                                   cATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTg                                                                                              <  2:897252/151‑1 (MQ=255)
                                                   cATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTg                                                                                              <  1:125956/151‑1 (MQ=255)
                                                   cATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTg                                                                                              <  2:105073/151‑1 (MQ=255)
                                                   cATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTg                                                                                              >  1:71458/1‑151 (MQ=255)
                                                            aaTATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGaaa                                                                                     <  2:418196/151‑1 (MQ=255)
                                                            aaTATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGaa                                                                                      >  1:650928/1‑150 (MQ=255)
                                                              tatTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAAc                                                                                   >  1:161358/1‑151 (MQ=255)
                                                                   ttATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGcc                                                                               <  1:347787/150‑1 (MQ=255)
                                                                     aTTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCAt                                                                             <  2:916175/150‑1 (MQ=255)
                                                                       tgctgcTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATc                                                                            >  2:534676/1‑149 (MQ=255)
                                                                       tgctgcTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCa                                                                           <  1:703588/150‑1 (MQ=255)
                                                                            ctgACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGcatc                                                                      >  2:297725/1‑150 (MQ=255)
                                                                              gACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGcatcat                                                                    <  2:105507/150‑1 (MQ=255)
                                                                              gACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGcatca                                                                     <  1:221488/149‑1 (MQ=255)
                                                                                  gTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCg                                                               <  1:914430/151‑1 (MQ=255)
                                                                                     cTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGcc                                                            >  1:761006/1‑151 (MQ=255)
                                                                                       gTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCt                                                           <  1:801477/150‑1 (MQ=255)
                                                                                                  acGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTTAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGc                                               <  1:290296/150‑1 (MQ=255)
                                                                                                   cGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGc                                               <  2:71454/150‑1 (MQ=255)
                                                                                                      aGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATg                                           >  1:603870/1‑151 (MQ=255)
                                                                                                         gCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCt                                         <  1:709721/150‑1 (MQ=255)
                                                                                                          ccACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTcg                                       <  2:979109/151‑1 (MQ=255)
                                                                                                          ccACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTcg                                       >  2:936444/1‑151 (MQ=255)
                                                                                                              aaTTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTcgccgc                                   >  2:714483/1‑151 (MQ=255)
                                                                                                                  gCCCGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCCCCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACt                                <  1:284605/150‑1 (MQ=255)
                                                                                                                    ccGGAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCg                              >  2:688133/1‑150 (MQ=255)
                                                                                                                      ggAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATg                           >  2:513038/1‑151 (MQ=255)
                                                                                                                      ggAAAATTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATg                           >  2:665582/1‑151 (MQ=255)
                                                                                                                        aaaaTTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGtt                         >  2:542793/1‑151 (MQ=255)
                                                                                                                         aaaTTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttc                        >  2:554022/1‑151 (MQ=255)
                                                                                                                         aaaTTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttc                        >  1:417392/1‑151 (MQ=255)
                                                                                                                          aaTTTCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcg                       >  2:994101/1‑151 (MQ=255)
                                                                                                                            tttCAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgct                     >  1:462458/1‑151 (MQ=255)
                                                                                                                               cAGCACATGGTGGCGCGTCCGGGTGCCGGGATGGCGGCGATTCAAAGAGCGATTGACAGAGTTTCTCCTTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGCTGCTCGCCGCACTCGTTGttcgcttcg                  >  2:299018/1‑151 (MQ=255)
                                                                                                                               cAGCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGttcgcttc                   <  2:637715/150‑1 (MQ=255)
                                                                                                                                 gCAAATGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTtcgcttcgct                <  1:931634/151‑1 (MQ=255)
                                                                                                                                   aaaTGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCttt              <  1:8068/151‑1 (MQ=255)
                                                                                                                                   aaaTGATGGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCttt              <  1:858468/151‑1 (MQ=255)
                                                                                                                                        atgGCGCGTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCt         <  1:808944/151‑1 (MQ=255)
                                                                                                                                            cgcgTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTgggg     >  2:857184/1‑151 (MQ=255)
                                                                                                                                               gTCAGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGAtt  <  1:746492/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GAACGCGGCGATGACGTTGCTCACCAACTTATTGACGCAGTTATCTAATGCCATGTCATCAATATTTTTATTGCTGCTGACGGTGCTGTTTATGCTGCTCGAAGTGCCACAATTGCCCGGAAAATTTCAGCAAATGATGGCGCGTCCGGTTGAAGGGATGGCGGCGATTCAACGTGCGATTGACAGTGTTTCTCATTATCTGGTGCTGAAAACAGCCATCAGCATCATCACCGGCCTGGTCGCCTGGGCGATGCTCGCCGCACTCGATGTTCGCTTCGCTTTTGTCTGGGGATT  >  NC_000913/1674302‑1674595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑