Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,248,431	0	A	C	43.3%	91.2 / 13.8	61	G34G (GGT→GGG)	lysP	lysine transporter
Reads supporting (aligned to +/- strand):  ref base A (25/9);  new base C (0/26);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 1.04e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACG  >  NC_000913/2248282‑2248579                                                                                                                                                      |                                                                                                                                                     gTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGAc                                                                                                                                                     <  1:330039/151‑1 (MQ=255)   ggCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCt                                                                                                                                                   <  1:644760/151‑1 (MQ=255)        aCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTAcc                                                                                                                                              >  2:147022/1‑151 (MQ=255)           aaCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGAt                                                                                                                                           >  1:50326/1‑151 (MQ=255)              cGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATgg                                                                                                                                         >  1:668898/1‑150 (MQ=255)                gAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAAc                                                                                                                                      <  1:46159/151‑1 (MQ=255)                  aaCCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCg                                                                                                                                    >  2:25979/1‑151 (MQ=255)                  aaCCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCg                                                                                                                                    >  1:546277/1‑151 (MQ=255)                   aCCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGc                                                                                                                                   <  2:496591/151‑1 (MQ=255)                       gCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAAt                                                                                                                               >  2:804951/1‑151 (MQ=255)                                   ccAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGt                                                                                                                   >  1:387190/1‑151 (MQ=255)                                    cAGTTCACGGAGAGGGGCCATCAGAAAGAAAACCATCAGCCCAATCAGAATAAACGAGAGAAAGGCCCCGCCCGGCCCTGCCTGAAAAATCGTTGCCCCAGAGGCAAAAAAAAGCCCTGCCCCGATGGAACCGCCAATGGCaa                                                                                                                          <  2:809601/143‑1 (MQ=255)                                     aGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTc                                                                                                                  >  1:170157/1‑150 (MQ=255)                                     aGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTc                                                                                                                  >  1:672289/1‑150 (MQ=255)                                     aGTCCACCGAGACTGGTAACCAGGAAGTAACCCACCAGCCCAATCAGCATATACGAAAGCAATCCCCCGCCCGGCCCTGCCTGAAAAATCGTTGCGCGAGAGGCAAAAAAAAGCCCTGTACGGAGGAAACCGCCAATGGCAATCATCGTCa                                                                                                                 <  2:727919/151‑1 (MQ=255)                                        tCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAgg                                                                                                               >  2:538788/1‑150 (MQ=255)                                         cACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTg                                                                                                             >  1:825593/1‑151 (MQ=255)                                            cGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGAcg                                                                                                          >  1:261805/1‑151 (MQ=255)                                             gagaCTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGAcgc                                                                                                         >  1:823489/1‑151 (MQ=255)                                                 cTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCt                                                                                                     >  2:303259/1‑151 (MQ=255)                                                       atcaGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAAtt                                                                                               >  2:560843/1‑151 (MQ=255)                                                           ggAAGTAAACCACCAGGCCAATCAGAATATAGGAGAAAAACCCCCCGCCCGGCCCTGCCTGAAAAATCGTTGCGCCAGAGGAAAAAAAAAGCCCTTTACGGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACg                                                                                           <  1:896823/151‑1 (MQ=255)                                                           gaaaGAAACCCAACAGCCAAATCAGCAAATGCGAGAGCAATCCCCCCCCCGGCCCTGCCTGAAAAATCTTGGCGCCAGAGGCAAAAAAAAGCCCTGTACCGATGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCa                                                                                             <  2:868705/147‑1 (MQ=255)                                                             aagaaacccaccggcccAACCAGCATATGGGAGAGCAATGCCCCGCCCGCACCTGCCTAAAAAATCGTTGCGCCAGAGGAAACAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACgg                                                                                          <  2:71004/135‑1 (MQ=255)                                                                     cATCAGGCCAATCAGAATATACGAGAGCAATGCCCCCCCCGGACCTGCCTGAAAAATCGTTGCGCCAGAGGCAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGcc                                                                                  <  1:723259/150‑1 (MQ=255)                                                                        cAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGgc                                                                              >  2:45656/1‑151 (MQ=255)                                                                              aaTCACAATATACGAGAGCAATCCCCCGCCCGGCCCTGCCAAAGAAATCGTTGCGCCAGAGGCAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGgc                                                                              <  2:755208/145‑1 (MQ=255)                                                                                 cAGCATATACGAGAGAAAGCCCCCGCCCGGCCCTGCCGGAGAAATCGTGGCGCAAGAGGCAAAAAAAAGCCCTGTACCGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCtg                                                                       <  1:441558/149‑1 (MQ=255)                                                                                   gCATATACGAGAGCAATCCCCCGCCCGGACCTGCCTGAAAAATCGTTGCGCCAGAGGCAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTgg                                                                    <  1:789805/150‑1 (MQ=255)                                                                                     atatACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGttt                                                                 >  1:331266/1‑151 (MQ=255)                                                                                       ataCGAGAGCAATGCCCCGCCCGGCCCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGCCCTGTACCGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGt                                                                   <  1:322239/147‑1 (MQ=255)                                                                                           gagaGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGttt                                                           >  2:497545/1‑151 (MQ=255)                                                                                             gagCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCg                                                         >  2:949428/1‑151 (MQ=255)                                                                                              aaaaaccccccccccGGCCCTGCCTAAGAAATCTTCGCCCGAGAGCAAAAAAAAAGCCTTGTCCAAATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTc                                                          <  2:833330/142‑1 (MQ=255)                                                                                               gCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCgg                                                        >  1:251202/1‑150 (MQ=255)                                                                                                 acgcccccGCCCGGACGTGCCTGAGAAATCGTTGCGCCAGAGGCAAAAAAAAGCCCTGTCCCGAGGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCgg                                                        <  2:40455/144‑1 (MQ=255)                                                                                                 aaccccccGCCCGGCCCTGCCTGAAAAACCGTTGCGCCAGAGGCAAAAAAAAGCCCTGTACCGAGGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGaa                                                      <  2:971250/146‑1 (MQ=255)                                                                                                     ccccccCGGGCCCTGCCTGAGAAACCGTGGCGCCAGAGGCAACAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAAc                                                    <  2:351625/148‑1 (MQ=255)                                                                                                     ccccGCCCGGCCCTGCCTGAAAAATCGTTGCGCCAGAGGCAAAAAAAAGACCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCAt                                                 <  1:799390/151‑1 (MQ=255)                                                                                                             ggACCTGCCTGAGAAATCGTTGCGCCAGAGGCAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAAt                                         <  1:147034/151‑1 (MQ=255)                                                                                                               aCCCGCCTAAAAAATCGTCGCTCGAGAGCAAAAAAAAAGGCTTAGCCCAAAGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAAt                                         <  2:669370/149‑1 (MQ=255)                                                                                                                cctgctggAGAAATCGTTGCGCCAGAGGCAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATg                                        <  1:488909/149‑1 (MQ=255)                                                                                                                cctgccggAAAAATCGTGGCGCCAGGGGAAAAAAAAAGCCCTGCACCGATGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACaaaa                                           <  2:143692/146‑1 (MQ=255)                                                                                                                cctgccggAAAAACCGTGGCGCAAGAGGCAAAAAAAAGACCTTTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCt                                      <  1:468290/151‑1 (MQ=255)                                                                                                                cctccctaAGAAATCGTGGCGCCAGAGCCAACAAAAACACCTGTCCCGATGACACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACaaaaa                                          <  2:854470/147‑1 (MQ=255)                                                                                                                 ctgcctgAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCt                                      >  1:519483/1‑150 (MQ=255)                                                                                                                    cctgAGAAATCGTTGCGCAAGAGGCAAAAAAAAGCCCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTAt                                    <  1:245873/149‑1 (MQ=255)                                                                                                                     ctgAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATcc                                  >  2:311094/1‑150 (MQ=255)                                                                                                                        agaAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCt                              >  2:557874/1‑151 (MQ=255)                                                                                                                        agaAATCGTTGCGCCAGAGGAAAAAAAAAGCCCTGTACCGATGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCt                              <  2:711697/151‑1 (MQ=255)                                                                                                                        agaAATCGTGGCGCCAGAGCAAAAAAAAAGCCCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATc                               <  2:713520/150‑1 (MQ=255)                                                                                                                           aaTCGTTGCGCCAGAGCCAAAAAAAAGCCCTGTCCGGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTaa                           <  2:584092/151‑1 (MQ=255)                                                                                                                             tCGTGGCGCCAGAGCCAAAAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTaaa                          <  2:953922/150‑1 (MQ=255)                                                                                                                                ttGCGCCAGAGCCAACAAAAAGCCCTGTCCCGAGGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAAt                       <  1:941954/150‑1 (MQ=255)                                                                                                                                tggcgcCAGAGGCAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAAt                       <  1:601041/148‑1 (MQ=255)                                                                                                                                     ccAGAGGCAAAAAAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAgg                   <  2:548971/149‑1 (MQ=255)                                                                                                                                             aacaaAAAGCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGgcg                 <  2:761673/143‑1 (MQ=255)                                                                                                                                             aacaaAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGt          >  1:873201/1‑150 (MQ=255)                                                                                                                                             aacaaAAACCCCTGTCCCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTa         <  1:417454/151‑1 (MQ=255)                                                                                                                                             aaaaaaaaGACCGGTCCCGAGGAAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGAt             <  2:50322/144‑1 (MQ=255)                                                                                                                                                    aGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACg  >  2:549050/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAACAAAAAGACCTGTACCGATGGAACCGCCAATGGCAATCATCGTCAGGTGACGCGCCTTTAATTCACGGCGTAAGCCCGGCGCTTCTGTGGTTTTAGTTTCGGAAACCATACAAAAATGCTATCCATCTTAAAAATGAGGCGCGATTGTAGCAGACG  >  NC_000913/2248282‑2248579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑