Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,903,692	0	C	A	44.3%	129.5 / 50.7	79	intergenic (+318/+1055)	ygcE/queE	putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (1/43);  new base A (35/0);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 1.11e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAA  >  NC_000913/2903545‑2903839                                                                                                                                                    |                                                                                                                                                      tttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCa                                                                                                                                                     <  1:717360/150‑1 (MQ=255)  ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGCGGCGGTTATGTAAAAcc                                                                                                                                                      <  2:847798/148‑1 (MQ=255)   tACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACt                                                                                                                                                   <  1:76178/150‑1 (MQ=255)          aaTATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGcc                                                                                                                                          <  1:581224/151‑1 (MQ=255)             atTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTTAAAACACT‑CAtttacccc                                                                                                                                        >  1:139547/1‑144 (MQ=255)             atTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑Ca                                                                                                                                      <  2:652954/151‑1 (MQ=255)             atTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑Ca                                                                                                                                      <  1:486001/151‑1 (MQ=255)              ttAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACC‑CATTTACCT‑c                                                                                                                                       >  1:952847/1‑149 (MQ=255)                 aCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAAc                                                                                                                                  <  2:299439/151‑1 (MQ=255)                  cTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACT‑CATTATCCT‑Caaaa                                                                                                                                   >  2:127855/1‑149 (MQ=255)                   tACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAAAC‑CATTAACCT‑Caaaaaa                                                                                                                                 >  2:175964/1‑148 (MQ=255)                    acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCGCTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACaa                                                                                                                                <  2:634326/150‑1 (MQ=255)                    acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACT‑CATTAACCT‑CAaaaaaa                                                                                                                                >  2:480662/1‑150 (MQ=255)                      acTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACC‑CATTATCCC‑CCAaaaaaaa                                                                                                                              >  1:497054/1‑149 (MQ=255)                       cTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACT‑CATTAACCT‑Caaaaaaaaaa                                                                                                                             >  2:171303/1‑148 (MQ=255)                         cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACaa                                                                                                                          <  2:537395/151‑1 (MQ=255)                             gTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAAAT‑CATTATACT‑CCAAACAAaaacaaacat                                                                                                                      >  2:968741/1‑149 (MQ=255)                                 ttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTcc                                                                                                                    <  2:338462/149‑1 (MQ=255)                                   aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACC‑CATTATACT‑CAAAAAAAAAAAAAACtt                                                                                                                      >  2:593859/1‑145 (MQ=255)                                    gAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACT‑CATTATCCT‑CCAAACAAAGACaaaaattcaaatt                                                                                                               >  2:184308/1‑141 (MQ=255)                                     aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGAttt                                                                                                              <  2:343264/151‑1 (MQ=255)                                      aTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATtta                                                                                                             <  2:731492/151‑1 (MQ=255)                                        aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATttatt                                                                                                           <  1:749188/151‑1 (MQ=255)                                          aaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACA‑CATTTAACT‑CAAAAAAAAAAAAAAAATTCAGAtttttt                                                                                                           >  2:944603/1‑149 (MQ=255)                                           aGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACC‑CATTAACCT‑Caaaaaaaaaaaaaaacttcaaatttttt                                                                                                           >  1:399191/1‑134 (MQ=255)                                           aGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACC‑CATTTGCCT‑CCAAACAAAAAAAAAATTACAGAtttttt                                                                                                           >  1:304932/1‑148 (MQ=255)                                             aCAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTa                                                                                                      <  2:167321/151‑1 (MQ=255)                                              cAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTaa                                                                                                     <  1:800006/151‑1 (MQ=255)                                              cAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTaa                                                                                                     <  1:92718/151‑1 (MQ=255)                                                  aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACT‑CATTATCCT‑CAAAAAAAAAAAAAAATTCCAGAATTATTATTTaa                                                                                                     >  2:251982/1‑147 (MQ=255)                                                      aTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAAATCCATTA‑CCT‑CCAAAAAAAAAAAAAATTCCAGAATTTTTTCtt                                                                                                       >  2:964129/1‑141 (MQ=255)                                                        cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAAt                                                                                            <  1:422974/150‑1 (MQ=255)                                                        cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACT‑CCTTAACCC‑CCAAAAAAAAAAAAACTTCCAGAATTTTTTCTTAAACaaataa                                                                                             >  1:961250/1‑145 (MQ=255)                                                          cACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAAc                                                                                         <  1:242672/151‑1 (MQ=255)                                                            cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACT‑CATTAACCT‑CAAAAAAAAAAAAAAATTCCCGATTTATTTCTTAAACAAAGAaaaaaa                                                                                        >  2:825772/1‑148 (MQ=255)                                                            cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACT‑CATTAACCT‑CAAAAAAAAAAAAAAATTCCCGAATTTTTTCTTTAACaaataaaaaaa                                                                                        >  1:781183/1‑141 (MQ=255)                                                              ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACAAT‑CATTAACCT‑CAAAAAAAAAACAAACCTCCAGATTTATTTCTTTAACAAAGAATAaaaa                                                                                       >  2:69531/1‑149 (MQ=255)                                                               tATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACA‑CAATATCCT‑CAAAAAAAAAACAAACTTCCCGATTTATTTATTAACCaaataaaaaaaa                                                                                       >  1:321752/1‑138 (MQ=255)                                                                 tCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAACT‑AATTAGCCT‑CAAAAAAAAAACAAAAATTCAAAATTTTTTCTTAAACAATAAATAACaaacat                                                                                   >  2:591802/1‑146 (MQ=255)                                                                      tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACAAT‑CCTTAACCT‑CAAAACAAAAAAAAACATTCAGATTTTTTACTTTAACAAAGAAAAAAAAACTTaaaaa                                                                              >  2:866537/1‑150 (MQ=255)                                                                       ttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTaaaaaa                                                                             <  1:104498/150‑1 (MQ=255)                                                                          aCTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACT‑CATTAACCT‑CAAAAAAAAAAAAAACATTCCGATTTATTTCTTAAACAATGAAAAAAAAACTTaaaaaaaa                                                                           >  2:772898/1‑147 (MQ=255)                                                                          aCTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAAtata                                                                         <  1:487178/151‑1 (MQ=255)                                                                          aCTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAAtata                                                                         <  2:9326/151‑1 (MQ=255)                                                                             tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACt                                                                       <  2:901804/150‑1 (MQ=255)                                                                              aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAACACT‑CATTAGCCT‑CAAAAAAAAAAAAAAATTCCAGATTTATTACTTAAACAAAGAAAAAAAAAATTaaaaaaaaaaattt                                                                     >  1:730648/1‑143 (MQ=255)                                                                                  tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAACTCCTTTACCCC‑CAAAAAAAAAAAAAAATCCCGAATTTTTTATTTAACAAAT‑AAAAAAAAACTTAAAAAAAATACTTTTAtt                                                                 >  1:841737/1‑151 (MQ=255)                                                                                    tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGGTTTTTTAAAACACC‑CATTAACCT‑CACAAAAAAGAAAAACTTCCACAATTATTAATTTAACAATGAAAAAAAAGCATTAAAAAAATACTGTTAtttt                                                               >  1:855325/1‑149 (MQ=255)                                                                                     gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTa                                                              <  2:147789/151‑1 (MQ=255)                                                                                          ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACT‑CCTTATCCTCCAAAAAAAAAAAAAACTT‑CAGATTTTTTATTTTAACAAAGAATAAAAAACTTAAAAAAAATAATTTTTTTTTaaaaa                                                          >  2:673669/1‑148 (MQ=255)                                                                                          ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaa                                                         <  2:8843/151‑1 (MQ=255)                                                                                            atatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACCCT‑CCTTTGCCT‑CCAAAAAAAAAAAAAATTCCAGATTTTTTACTTAACCAAAGAATAAAAAACTTAAAAAAAATAATTTTTTTTTAaaaaaa                                                        >  1:106507/1‑150 (MQ=255)                                                                                              atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACT‑ACTTATACT‑CAAAAAAAAAAAAAAATTTCACATTTTTTTTTTTAAAAAAGAAAAACAAAATTAAAAAAAATAATGTTATTTTTaaaaaaaa                                                      >  2:871680/1‑150 (MQ=255)                                                                                              atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACA‑CATTATCCT‑CCAAAAAAAAACAAACTTCCAGATTTTTTAATTTAACAAAGAAAAAAAAACTTTAAAAAAATAATTTTATTTTAAAGaaaaa                                                      >  1:871836/1‑150 (MQ=255)                                                                                                  ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAAtt                                                   <  2:179915/149‑1 (MQ=255)                                                                                                  ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAAt                                                    <  1:503332/148‑1 (MQ=255)                                                                                                      tCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACC‑CATTATCCT‑CAAAAAAAAAAAAAAATTCCAGATTTTTTAATTAAACAAAAAATAAAAAAATTAAAAAAAATAATGTTATTTTTAAAAAAAAAATaaaa                                               >  1:521013/1‑147 (MQ=255)                                                                                                      tCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAAAT‑CATTTACCT‑CAAAAAAAAGACAAAAATTCAGAATTATTACCTAACCAAACAAAAAAAAACATTAAAAAAAAACTTTTATTTTAAAAAAAAAAATaaaa                                               >  1:92486/1‑147 (MQ=255)                                                                                                       cATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAgg                                             <  1:938416/150‑1 (MQ=255)                                                                                                          aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAtt                                         <  2:478289/151‑1 (MQ=255)                                                                                                           aGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTa                                        <  1:814118/151‑1 (MQ=255)                                                                                                              aCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAtt                                     <  2:107225/151‑1 (MQ=255)                                                                                                              aCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAtt                                     <  1:246909/151‑1 (MQ=255)                                                                                                                 aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTc                                    <  1:37083/149‑1 (MQ=255)                                                                                                                 aTTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCaa                                  <  2:338619/151‑1 (MQ=255)                                                                                                                   tcatcaACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAat                                 <  1:243432/150‑1 (MQ=255)                                                                                                                    catcaACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAatat                               <  1:171316/151‑1 (MQ=255)                                                                                                                    catcaACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAata                                <  2:420129/150‑1 (MQ=255)                                                                                                                     atcaACAGCGTATAGAGGCGGTTATGTAAAAACACC‑CATTAGCCT‑CCAAAAAAAAACAAAATTCCAGATTTTTTTATTAAACAAAGAAAAACAAACTTAAAAAAAATAATGTTATTTTAAAAAAAAAAATAAAAAGCAATAATTTAAAAtt                              >  1:621397/1‑151 (MQ=255)                                                                                                                       caACAGCGTATAGAGGCGGTTTTGTAAAAACACA‑CATTTTCCT‑CAAAAAAAAAACAAAAATTCCGAATTTTTATTTAAACAAATAAAAAAAAACTTAAAAAAAATAATTTTTTTTTTAAAAAAAAAATAAAAAGAATTAATTTAATAtt                              >  2:76989/1‑149 (MQ=255)                                                                                                                       caACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTa                             <  2:619702/150‑1 (MQ=255)                                                                                                                             cGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaat                      <  1:875759/151‑1 (MQ=255)                                                                                                                             cGTATAGAGGCGGTTATGTAAAAAAACC‑CATTAGACT‑CAAAAAAAAAAAAAACATTCCGATTTTTTTATTAAACAAAGAAAAAAAAACTTAAAAAAAATAATTTTTTTTTTAAAAAAAAAATAAAAAGCAATAATTTAAAATTaaaaaaa                       >  2:673311/1‑150 (MQ=255)                                                                                                                                      gCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa             <  2:501067/151‑1 (MQ=255)                                                                                                                                            aTGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAttt       >  1:955828/1‑151 (MQ=255)                                                                                                                                            aTGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAtt        <  1:252003/150‑1 (MQ=255)                                                                                                                                              gTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTc      <  2:719546/150‑1 (MQ=255)                                                                                                                                                aaaaCCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTa   <  2:23867/151‑1 (MQ=255)                                                                                                                                                aaaaCCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTa   <  1:282276/151‑1 (MQ=255)                                                                                                                                                 aaaCCACT‑CATTAGCCT‑CAAAACAAATACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTaa  <  1:224308/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACT‑CATTAGCCT‑CAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAA  >  NC_000913/2903545‑2903839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑