Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,238,0490AG42.9% 119.8 / 21.7 77E139G (GAA→GGA) yjbHDUF940 family extracellular polysaccharide protein
Reads supporting (aligned to +/- strand):  ref base A (7/37);  new base G (33/0);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 5.75e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACAGCAGCGTCGAAGCGTTCTCTGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTACAAACAGGCGGG  >  NC_000913/4237900‑4238199
                                                                                                                                                        |                                                                                                                                                          
gTACAGCAGCATCGAAGCGTTCTCTGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGaa                                                                                                                                                           <  2:947792/151‑1 (MQ=255)
     gcagcGTCGAAGCGTTCTCTGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTTGTGCGGGATATCt                                                                                                                                                      >  1:769062/1‑151 (MQ=255)
       agcGTCGAAGCGTTCTCTGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCttg                                                                                                                                                    <  1:803048/151‑1 (MQ=255)
       agcGTCGAAGCGTTCTCTGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTGTTGGGGGATATCtt                                                                                                                                                     >  2:776782/1‑150 (MQ=255)
          gTCGAAGCGTTCTCTGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGGTATCGGC‑GG‑GAC‑GGGGGGGTTTGATGCGGGATATCtt                                                                                                                                                     >  2:337453/1‑147 (MQ=255)
                   ttCTCTGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑CGGGGTGTTTGATGCGGGATATATTTTT‑TCCAG‑G‑aaa                                                                                                                                      >  2:203855/1‑150 (MQ=255)
                      tctGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTTATGCGGGATATATTGTT‑TGCAG‑C‑AAA‑gac                                                                                                                                  >  1:296386/1‑148 (MQ=255)
                       ctGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑Gcc                                                                                                                                  <  1:788446/149‑1 (MQ=255)
                       ctGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTGGTGGGGGATATTTTTTT‑GGCCG‑C‑AAA‑Acc                                                                                                                                  >  1:443607/1‑149 (MQ=255)
                            gATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGc                                                                                                                           <  1:891457/151‑1 (MQ=255)
                             aTCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTTATGCGGGATATCTTTTT‑GCCCG‑C‑AAA‑ACCCggggg                                                                                                                            >  1:672244/1‑149 (MQ=255)
                               cAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCg                                                                                                                         <  2:850581/150‑1 (MQ=255)
                                 aaCGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTGGTGCGGGATATCTTGTT‑GCCCG‑C‑AAA‑ACCTGGGGGcc                                                                                                                          >  1:465635/1‑147 (MQ=255)
                                  aCGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTGGTGGGGGATATTTTTTT‑TGCAGCC‑AAA‑ACCTGGGGGCGGt                                                                                                                        >  2:888531/1‑149 (MQ=255)
                                  aCGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGGTTTATGGGGGATATCTTTTT‑TCCCG‑C‑AAA‑GCCTGGGGGGCg                                                                                                                         >  2:12967/1‑147 (MQ=255)
                                    gTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGGTATCGGC‑GG‑GAA‑GGGGGCGTTTTGTGGGGGATAACTTTTT‑GGCCG‑C‑AAA‑ACCCGGGGGGCggttgg                                                                                                                    >  2:560446/1‑145 (MQ=255)
                                     tataAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTTATTCGGGATATCTTTTT‑TGCCG‑C‑CAA‑ACCCGGGGGGCGtttggtt                                                                                                                  >  1:400294/1‑146 (MQ=255)
                                      ataaagataaagCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGGTTTATGCGGGATATCTTTTT‑TGCCG‑C‑AAA‑ACCCGGGGGGCGGTCGAttt                                                                                                                 >  2:890477/1‑151 (MQ=255)
                                          agataaagCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGCGTTTTGTGGGGGATATTTTGTT‑GCCCG‑C‑CAA‑ACCCGGGGGGCGGTCGGtttt                                                                                                                >  2:785330/1‑148 (MQ=255)
                                            ataaagCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTTTTGCGGGATATCTTGTT‑GGCCG‑C‑AAA‑ACCTGGGGGCCGGTCGGTTTTAcac                                                                                                            >  2:347935/1‑148 (MQ=255)
                                            ataaagCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAA‑GGGGGTGGTTTGTGGGGGATATTTTTTT‑GGCCG‑C‑AAA‑ACCTGGGGGGCGTTCGGttttttgc                                                                                                            >  2:425078/1‑146 (MQ=255)
                                             taaagCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTTGTGCGGGATATCTTTTT‑GGCCG‑C‑AAA‑ACCTGGGGGGCGTTCGGTTTTACg                                                                                                             >  1:195095/1‑148 (MQ=255)
                                              aaagCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGCGGG‑TCC‑GGGGCGGTTTG‑TGGGGGATATCCTGTT‑GGCAG‑C‑AAA‑GCCTGGGGGGCGTTCGGTTTTTCGCTc                                                                                                          >  2:282151/1‑150 (MQ=255)
                                               aagCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGGTATCGGC‑GG‑GAC‑GGGGGTGTTTGGTGGGGGATATTTTTTT‑TGCCG‑G‑AAA‑ACCTGGGGGGCGGTTGGTTTTTTGCTCgg                                                                                                        >  2:853495/1‑151 (MQ=255)
                                                agCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GACGGGGGCGGTTTG‑TGGGGGATATCTTGTT‑GGCCG‑CAAAA‑GCCGGGGGGCGGTTGGTTTTTA‑GCTCgg                                                                                                        >  2:213061/1‑150 (MQ=255)
                                                agCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTTGTGGGGGATATTTTTTT‑GGCAG‑C‑AAA‑ACCTGGGGGGCGGTCGGTTTTTCGCTccg                                                                                                        >  2:687063/1‑148 (MQ=255)
                                                agCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGGGGTTTGTGCGGGGTATTTTGTT‑GGCAG‑G‑CAA‑GACTGGGGGGCGGTTGGtttttagacc                                                                                                          >  2:500023/1‑142 (MQ=255)
                                                  ccTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTGATGCGGGATATCTTGTT‑GCCCG‑C‑AAA‑ACCCGGGGGGCGGTCGATTTTACGCCCGGcc                                                                                                      >  1:889400/1‑150 (MQ=255)
                                                            cAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGGTATCGGC‑GG‑GAC‑GGGGGCGTTTTGTGGGGGAAATCTTGTT‑GCCCG‑C‑CAA‑AGCTGGGGGGCGTTTGATTTTTTGCTCGGGCTGGGGTtgggg                                                                                           >  2:740206/1‑151 (MQ=255)
                                                            cAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCctgggctggg                                                                                             <  2:402162/149‑1 (MQ=255)
                                                            cAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCctgggctggg                                                                                             <  1:967481/149‑1 (MQ=255)
                                                             aaaCTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGt                                                                                          <  1:875122/151‑1 (MQ=255)
                                                             aaaCTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGt                                                                                          <  1:801402/151‑1 (MQ=255)
                                                              aaCTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTa                                                                                         <  1:407770/151‑1 (MQ=255)
                                                                   cGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑CGGGCTGTTTTGTGCGGGATATCTTGTT‑GCCAG‑C‑AAA‑ACCTGGGGGCCGTTTGATTTTTCGCTCGGCCTTGGGTgggggggttt                                                                                      >  1:869678/1‑144 (MQ=255)
                                                                        gtgGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGGGCGGGGTATCGGC‑GGTTCC‑GGGGCTGTTGTAT‑CGGGATAACCTTTT‑GTCAG‑C‑AAACGCCGGGGGCCCGTT‑GATTTTTCGCTTCGCCCGGGGGGGGGGGGCTTGGGGAcc                                                                               >  2:505187/1‑151 (MQ=255)
                                                                          gggAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGGCGTTTTATGCGGGATATTTTGTT‑GCCCG‑C‑AAA‑ACCCGGGGGGCGGTCGGTTTTTCCCCCTGCCTGGGCTGGGGGGATTTTGGCAcc                                                                               >  2:140360/1‑149 (MQ=255)
                                                                            gAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCg                                                                           <  2:732786/151‑1 (MQ=255)
                                                                             aaGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCgg                                                                          <  1:754558/151‑1 (MQ=255)
                                                                               gagagTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTa                                                                        <  2:967009/151‑1 (MQ=255)
                                                                                  agTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAAtg                                                                     <  1:967389/151‑1 (MQ=255)
                                                                                  agTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑CGGGGTGTTTTGTGGGGGAAATCTTGTT‑TGCAG‑G‑CAA‑ACCCGGGGGGCGGTCGGTTTTTCGCCCCGCCTGGGGTGGGGGGATTTTGGGACCAACGGGaa                                                                       >  2:948338/1‑149 (MQ=255)
                                                                                   gTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTGGTGCGGGATATTTTGTT‑GGCCG‑C‑AAA‑GCCTGGGGGGCGGTTGGTTTTACGCTCGGCCTGGGCTGGGGGGATTTGGGCACCAGCGGGaa                                                                       >  1:604031/1‑148 (MQ=255)
                                                                                       cTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGCTGGTTTATGCGGGATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGGCGGTCGATTTTTAGCTCGGCCTGGGCTGGGGGGATTTTGGCACCAGCGGGAAtgtgga                                                                 >  1:77549/1‑148 (MQ=255)
                                                                                        tGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTTGTGGGGGAAATCTTGTT‑GCCAG‑C‑AAA‑ACCTGGGGGGCGTTCGGTTTTTTGCTCGGCCTGGGGGGGGGGGATTTGGGCACCAGCGGGAATGTGgaa                                                                >  2:649703/1‑150 (MQ=255)
                                                                                         ggCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGaaaa                                                               <  1:62898/150‑1 (MQ=255)
                                                                                          gCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAAt                                                             <  1:697895/151‑1 (MQ=255)
                                                                                          gCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAAt                                                             <  1:12969/151‑1 (MQ=255)
                                                                                            tGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑GGGGGTGTTTTGTGGGGGAAATCTTGTT‑GGCAG‑C‑CAA‑ACCTGGGGGGCGTTCGATTTTTCGCTCGGGCCGGGGTGGGGGGGTTTTGGCCCCCGCGGGAAAGGGAAAAATcc                                                           >  1:579178/1‑151 (MQ=255)
                                                                                               cGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGct                                                        <  1:200041/151‑1 (MQ=255)
                                                                                                   aGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGt                                                    <  2:244018/151‑1 (MQ=255)
                                                                                                    gTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑GAC‑CGGGGGGTTTTGTGGGGGAAATTTTTTTGGCCGC‑C‑AAA‑CCCGGGGGGCGGTTGGTTTTTA‑GCCCGGCCCTGGGTGGGGGGATTTTGGGACCCGCGGGAATGTGGAAAAACCGCCCTGtt                                                   >  1:156560/1‑151 (MQ=255)
                                                                                                      ggCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTc                                                  <  1:425120/150‑1 (MQ=255)
                                                                                                      ggCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCa                                                 <  2:672179/151‑1 (MQ=255)
                                                                                                        cGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAg                                                >  2:160789/1‑150 (MQ=255)
                                                                                                          gTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCa                                             <  1:495092/151‑1 (MQ=255)
                                                                                                            tGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGGATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGcc                                              >  2:435014/1‑148 (MQ=255)
                                                                                                                gcgcGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGAt                                        <  1:689483/150‑1 (MQ=255)
                                                                                                                gcgcGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATa                                       <  2:420359/151‑1 (MQ=255)
                                                                                                                 cgcgGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATaa                                      <  1:160801/151‑1 (MQ=255)
                                                                                                                    gggATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAata                                   <  1:953392/151‑1 (MQ=255)
                                                                                                                      gATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATAtt                                 <  1:776857/151‑1 (MQ=255)
                                                                                                                      gATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATAtt                                 <  1:775800/151‑1 (MQ=255)
                                                                                                                       atatCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAat                                    <  2:91449/147‑1 (MQ=255)
                                                                                                                        tatCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGGATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATAtt                                 >  1:812912/1‑149 (MQ=255)
                                                                                                                            ggc‑gg‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATc                           <  1:147736/151‑1 (MQ=255)
                                                                                                                                 g‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGa                       <  2:400255/151‑1 (MQ=255)
                                                                                                                                    aC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGAt                      <  1:453912/150‑1 (MQ=255)
                                                                                                                                        gggCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACa                  <  1:538377/151‑1 (MQ=255)
                                                                                                                                           cTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCt               >  1:762239/1‑151 (MQ=255)
                                                                                                                                             gTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTa              >  1:810093/1‑150 (MQ=255)
                                                                                                                                               ttGCTGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTACa            >  1:555303/1‑150 (MQ=255)
                                                                                                                                               ttGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTACa            <  1:524478/150‑1 (MQ=255)
                                                                                                                                                tGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGGTACaa           <  2:310399/150‑1 (MQ=255)
                                                                                                                                                tGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTACaa           <  2:719714/150‑1 (MQ=255)
                                                                                                                                                tGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTACaa           <  1:835051/150‑1 (MQ=255)
                                                                                                                                                  aTGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTACAAAc         >  1:68200/1‑150 (MQ=255)
                                                                                                                                                      ggAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTACAAACAGGCg    <  1:560501/151‑1 (MQ=255)
                                                                                                                                                        aaTATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTACAAACAGGCggg  >  1:259124/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                          
GTACAGCAGCGTCGAAGCGTTCTCTGGCGATCAAACGTATAAAGATAAAGCCTTCGATCTCAAACTGCGTTTGTGGGAAGAGAGTTACTGGCTGCCGCAAGTGGCGGTTGGTGCGCGGGATATCGGC‑GG‑TAC‑GGGGCTGTTTGATGCGGAATATCTTGTT‑GCCAG‑C‑AAA‑GCCTGGGGGCCGTTCGATTTTACGCTCGGCCTGGGCTGGGGGTATTTGGGCACCAGCGGTAATGTGAAAAATCCGCTCTGTTCAGCCAGTGATAAATATTGCTATCGCGATAACAGCTACAAACAGGCGGG  >  NC_000913/4237900‑4238199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑