| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 45.7% | 30.8 / 17.6 | 35 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (14/5); new base A (0/16); total (14/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.65e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCC > NC_000913/357742‑358031 | gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca > 1:57246/1‑150 (MQ=255) gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg < 1:788380/151‑1 (MQ=255) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgcc > 2:190988/1‑151 (MQ=255) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgcc < 2:468924/151‑1 (MQ=255) aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCACCGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc > 2:889856/1‑150 (MQ=255) aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc > 2:210757/1‑150 (MQ=255) gtcAATCCCCGCCGCGGACTTGTGTCAGCGGTTCCCGTGATTCTTTCCCGCAAAAACCCGCGCTTTTACCCACCCCCATGTGAAAAAATGCCTTCGCGGCGGCTGTTTGCCAGCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTcc < 2:826632/147‑1 (MQ=255) ggCATTTCCAGCCGCAGACCTGTGTCAGCGGTTACCGTGTTTCTTTCCCCCCAACACCCGCGCTTTTATCCAACCCCTTGTCCAAAAATGCTTTCGCGGCGGCTGTCTGCCAGTTATTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCag < 1:133909/151‑1 (MQ=255) ccgcccGCAGACTTGTGTCAGCGGTTACCGTGTTTCATTCCCGCAAACAACCGCGTATTTACCCACCCCCTTGTGAAAAAATGCTTTCGCGGGGGTTGTGGCCCGGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTg < 1:876416/145‑1 (MQ=255) ccAGCCGCAGACCTGTGTCGGCGGCTCCCGTGATTCATTCCCGCCAACACCCGCGCTTTTACCCAACGCCATGTGCAAAAAGGCCTTCGGGGCGGCTGTCTCCCAGCTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGc < 1:384871/151‑1 (MQ=21) ccACCCGCAGACTTGTGTCGGCGGCTCCCGTGATTCATTCCCGCAAACACCCGCGATTTTACCCAACGCAATGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTTTTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTg < 1:388794/149‑1 (MQ=255) cAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCg > 2:361941/1‑151 (MQ=255) ccGCAGACTTGGGTCAGCGGCTACGGGGTTTCATTCCCGCCAAAACCCGCGAATTTACCCAACGCCTTGTGCAAAAATCCTTTCGGGGCGGCTGTCGGCCAGCTATTTTTCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg < 1:780763/150‑1 (MQ=255) gccttgtgTCGGGGGTTCCCGTGATTCTTTCCCCCCACCACCCGCATTTTTACCCAACGCAATGTGAAAAAATCCCTCCGCGGCGGCTGTCTGCCAGTTTTTTTCCCCCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc < 2:396274/143‑1 (MQ=255) tgtCGGCGGCTCCCGTGTTTCATTCCCGCAAACACCCGCGCTTTTACCCACCGCCTTGTGCAAAATTCCCTCCGGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAagag < 2:680053/146‑1 (MQ=255) cAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggggaggaaaa > 2:930962/1‑137 (MQ=255) tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGaaaaaaattttaacc > 1:763394/1‑139 (MQ=25) ccGCGCTTTTACCCAACGCCATGTGAAAAAATGCCTCCGCGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCgct < 1:403237/149‑1 (MQ=255) gcgcATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAGAG‑TTTAAACCCG‑CCTCTTGTGT‑gggcaaacgt > 1:732623/1‑139 (MQ=25) gcgcATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAAAG‑A‑TTTTAACCCCTCATTTTTTGT‑GGGGATAgctggt > 1:191047/1‑148 (MQ=37) aTTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGAAAAAA‑A‑TTTTAACCCCCCCTGTTTTGT‑GGGAAACgc > 1:961632/1‑142 (MQ=25) tttACCCACCCCCATGTGAAAAAATGCTTTCGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGctgctg < 1:700445/146‑1 (MQ=255) gtgCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGGGAAAAAA‑CATTTAGGCCCGCCTTGTT‑TTT‑GGGGATCCCTGCCGG‑TGAAAAAGTGGGAAggg > 2:16453/1‑149 (MQ=255) aaaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGAAAAAA‑C‑TTTTATCCCCTCGTGCTGTGT‑GGGGATAGCCGCTGG‑GAACAAAATGGGAAggg > 1:158137/1‑145 (MQ=255) aaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGAAAAAA‑A‑TTTTAACCCCTCCTTTTTTGT‑GGGGAACGCCGCTGGTTAAAAAATGGGAAAGGGAAgggg > 1:801401/1‑148 (MQ=255) ggggcggcTGTCTGCCGGTTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCa < 2:386356/146‑1 (MQ=255) gcggcgGCTGTCTGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCt < 1:475990/151‑1 (MQ=255) gcggcgGCTGTCTGCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCa < 2:486121/148‑1 (MQ=255) ggggcTGTCGCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATc < 2:43970/142‑1 (MQ=255) cggcTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAGAGAAAAAA‑A‑TTTTAAACCCTCCCGTTGTGG‑GGGGAACGCTGCTGG‑GGACAAAGTGGGAAGGGAAGAGGGGCGGATCAGCGcc > 1:907797/1‑149 (MQ=255) ggcTTTCTGCCGGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGa < 1:890691/151‑1 (MQ=255) tcgcccggtttttttcCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAcc < 2:741775/138‑1 (MQ=255) tGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGGGGAATAA‑C‑TTCTAATCCATCTTTTTGTGTGGGCAATC‑CCGGTGG‑GTACAATTTTGAAAGGGGAGGGCGGGGGATGAGcccccacaccacgc > 2:15968/1‑141 (MQ=255) ttttttcCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAtt < 1:767249/147‑1 (MQ=255) cccgccgAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCg < 2:688580/149‑1 (MQ=255) ccgccgCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCg < 1:566007/149‑1 (MQ=255) caacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAA‑A‑TTTAAACCCCCCCTCTTTTGT‑GGGGATCTCTTCTTG‑TTACAAAGTGGGAAGGGGAGGGGGGCGGAACAGCTCCCGAACCCCGCCAATTTAGTTTGcc > 2:959516/1‑145 (MQ=255) aacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAGAGAAAAAA‑A‑TTTAAACCCCTCCCCTTGTGT‑GGGGAACGCTGCTGG‑GTACAAAGTGGGAAGGGGAGGGGGGCGGAACAGCTCCACCACCGCGCCAAtt > 2:283851/1‑135 (MQ=255) | GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAG‑C‑TTTAAGCCCGTCATGTTGTGT‑GGCAATCGCTGCTGG‑TAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCC > NC_000913/357742‑358031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |