Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,337,723 | 0 | A | C | 46.3% | 64.2 / 16.3 | 56 | G566G (GGT→GGG) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
Reads supporting (aligned to +/- strand): ref base A (17/12); new base C (0/25); total (17/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.12e-06 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTAC > NC_000913/2337574‑2337868 | ccGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTa > 1:880174/1‑150 (MQ=255) gcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCttt < 2:626804/151‑1 (MQ=255) gcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCttt < 1:416065/151‑1 (MQ=255) cgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTc < 2:548462/151‑1 (MQ=255) cgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTc < 2:455878/151‑1 (MQ=255) gcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTcc > 2:113794/1‑151 (MQ=255) aGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCacg > 1:551721/1‑150 (MQ=255) tGGCTTCCGGCAACTGATAAATTTTCATCGAATAGACGCGACCACGGCGGGAGAAGCACAGAATATGGTCGTGGGTGTTCGCCCCCAGCAGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCacga < 2:923668/149‑1 (MQ=255) ggtttCCGGCAACTAAAAAATTTTCATCAAATAGCCGCGCCCCCGGCGGGAAAAGCACAAAATATGGTCGGGAGTGTCCCCCACCAGCAGTCGGTCAATAAAGTCTTCTTTTTTAATCCGTGCGGCAGTTTTACCTTTCCCGCCacgacg < 2:869511/147‑1 (MQ=255) cTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTg > 1:528997/1‑151 (MQ=255) ttCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTgc > 2:379667/1‑151 (MQ=255) ccGGCAACTGATAAACTTTCATCAAATAGACGCGCCCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGAAGATTTACCTTTCCCGCCACGACGCTgc < 1:544398/149‑1 (MQ=255) ggCAACTGAAAAACTTTCATCGAATAGACGCGCCCACGGCTGGAGAAGCACAGAATATGGTCGGGGGTGTCCCCCCCCAGCAGTCGGTCAATAAAGTCTTCTTCTTTAATACTTGGGGCAGTTTTCCCTTTCCCGCCACGACGCt < 2:873382/145‑1 (MQ=255) aaCTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGt > 2:489412/1‑151 (MQ=255) aacttttCATCGAATAGCCGCGCCCCGGGCTGGAGAAGCACAGAATATGGTCGTGTGTGTTCGCCCCCAGCAGTCGGTCGAAAAAGTCTTCTTTTTTAATACGTGCGGCAAATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGa < 2:677057/147‑1 (MQ=255) aaaCTTTCATCGAATAGCCGCGCCCCGGGCGGGGAAAGCAAAAAATATGGTCGTGATTGTTCGCCCCCAGCAGTCGGTCGATAAAGTCTTTTTCTTAAATCCGTGCGGCAGATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGa < 2:643756/151‑1 (MQ=255) ttCATCGAATAGACGCGCCCACGGCTGGAAAAGCACAGAATATGGTCGTGATTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAg < 1:448185/149‑1 (MQ=255) aTAGACGCGCCCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTTTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATAc < 1:542784/151‑1 (MQ=255) aTAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATAc > 1:237154/1‑151 (MQ=255) aGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGGCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACtt > 1:305938/1‑151 (MQ=255) aCGCGACCCCGGCTGGAGAAGCACAGAATAGGGTCGTGAGTTTTCGCCACCAGCAGTCGGTCAATAAAGTCTTCTTCTTTAATCCGTGCGGAAGATTTACCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACt < 2:937332/148‑1 (MQ=255) gcgcCCCCGGCTGGAGAAGCACAGAATATGTTCGTGAGTTTTCGCCCCCAGCAGTCGGTGGATAAAGTCTTCTTTTTTAATTCGGGCGGCATATTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATGCt < 2:631053/146‑1 (MQ=255) cgcccACGGCTGGAGAAGCACAGAATATGGTCGTGAGTTTCCGCCCCCAGCAGTCGTTCAATAAAGTCTTCTTCTTTAATACGTGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAAc < 2:74957/146‑1 (MQ=255) gACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGt > 2:425317/1‑150 (MQ=255) ccACGGCGGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCCCCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGt < 1:580300/148‑1 (MQ=255) ggCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCCCCACCAGCAGGCGGTCGATAAAGTTTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTg < 1:316699/151‑1 (MQ=255) cTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGt > 1:525371/1‑151 (MQ=255) tGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTg > 1:644707/1‑151 (MQ=255) ggAGAAGCAAAAAATTTGGTTGTGGGTGTTCGCCACCAGCGGTCGGTCAATAAAGTTTTCTTTTTAAATCCGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGt < 1:407602/149‑1 (MQ=255) aaccacaGAATATGGTCGTGAGTGTCCGCCGCCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATGCGTGCGGAAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGt < 2:395312/142‑1 (MQ=255) aaGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag > 1:660254/1‑148 (MQ=255) aaGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag > 2:280532/1‑148 (MQ=255) aaGCACAAAATATGGCCGTGAGTGTTCGCCCCCAGCAGTCGGTCGATAAAGTCTTCTTCTTTATTCCGTGGGGCAGATTTTCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgaga < 2:218554/149‑1 (MQ=255) caaaGAATATGGTCGTGAGTGTTCGCCCCCAGCAGTCGGTCGATAAAGTCTTCTTCTTAAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGagagag < 1:432445/146‑1 (MQ=255) acaAAATATGGTCGTGAGTGTTCGCTCCCAGCGGTCGGTCGAAAAAGTTTTCTTTTTTAATCCGGGGGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGagagag < 2:857987/148‑1 (MQ=255) caGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTc > 1:118468/1‑151 (MQ=255) cGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATc > 2:723962/1‑151 (MQ=255) tGAGTGTCCCCCCCCAGCATTCGGTCGATAAATTCTTCTTCTTTAATACGTGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACAt < 2:643257/148‑1 (MQ=255) tgtTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTg > 2:269659/1‑151 (MQ=255) ttCGCCCCCAGCAGTCGGTCAAAAAAGTCTTCTTTTTTAATACGTGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTg < 1:786367/149‑1 (MQ=255) tcggCCACCAGCGGTGGGTCAATAAGTTCTTTTTCTTTACTAGGTGGGGAAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTgg < 2:800120/147‑1 (MQ=255) tccGCCCCAAGCAGTTGGTCGATAAACTTTTTTTTTTAAAGGCGTGCGAAAATTTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTg < 1:561749/147‑1 (MQ=255) cGCCCCCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATCCGTGCGGCATATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTg < 2:269386/151‑1 (MQ=255) gCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTg > 2:127848/1‑150 (MQ=255) ccaccaGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTggg < 1:795012/147‑1 (MQ=255) ggtcgATAAAGTCTTCTTTTTTAATACGTGCGGCAAATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc < 2:716255/149‑1 (MQ=255) cgAAAAAGTCTTCTTCTTTAATACGTGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGt < 2:613503/150‑1 (MQ=255) gaaaaaGTCTTCTTCTTTAATACGTGCGGCGGTTTTTCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAgg < 1:562591/145‑1 (MQ=255) aaaTTCTTCTTCTTTAATCCGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGAt < 1:904530/150‑1 (MQ=255) aaaGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATg < 1:179626/151‑1 (MQ=255) ttcttcttTAATCCGTGCGGAAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCt < 2:160585/148‑1 (MQ=255) ttttatccgtcaggCAGATTTCCTTTTTCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCt < 1:50951/135‑1 (MQ=255) tAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGc > 2:345143/1‑151 (MQ=255) gggCGGCATTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTg < 2:830064/147‑1 (MQ=255) gggCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGt < 2:551656/146‑1 (MQ=255) aGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGt < 1:511772/150‑1 (MQ=255) aTTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTac < 1:526509/150‑1 (MQ=255) | CCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTAC > NC_000913/2337574‑2337868 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |