| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 231,694 | 0 | C | T | 49.1% | 46.8 / 25.6 | 56 | F191F (TTC→TTT) | yafD | endo/exonuclease/phosphatase family protein |
| Reads supporting (aligned to +/- strand): ref base C (10/18); new base T (27/0); total (38/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.24e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATTACCTGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTC‑GGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTG > NC_000913/231548‑231842 | ccATTACCTGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATg < 2:490813/151‑1 (MQ=255) aTTACCTGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGcc < 1:133186/151‑1 (MQ=255) cTGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTCATGCCTgg > 1:616506/1‑149 (MQ=255) tGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGG‑GATTTTAATGcc > 2:106650/1‑145 (MQ=255) cccGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGG‑GAATTTAATGCCTCGAGcc > 2:4007/1‑147 (MQ=255) ccGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATGCCTGGAg > 1:82308/1‑144 (MQ=255) ccTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAgg < 1:114966/151‑1 (MQ=255) ccTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAgg < 1:471663/151‑1 (MQ=255) cTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATGCCTGGGGCCGGAGAAgg > 1:178292/1‑150 (MQ=255) tGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGa < 2:215379/150‑1 (MQ=255) tggtggTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GGTTTTAATGCCTGGGGCCGTAGAAGGATGaa > 1:255574/1‑148 (MQ=255) gtggtTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGt < 2:391611/151‑1 (MQ=255) atatACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGGCGTAGAAGGATGAACGCGGTatat > 2:269221/1‑149 (MQ=255) cacGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTg < 1:311607/151‑1 (MQ=255) cacGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTg < 2:353649/151‑1 (MQ=255) ccGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGG‑GATTTAAAT‑CCTTGGGGCGCAGAAGGATGAAAGCGGTATATCGCctt > 2:434820/1‑146 (MQ=255) cGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATTCCTGGGGGCGGAGAAGGGTGAACGCGTTATATCGCTTTTCGC‑gg > 1:691721/1‑151 (MQ=255) cGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑gg < 1:272364/151‑1 (MQ=255) aaCTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGG‑GGTTTTAATTCCTCGGGGCGGAGAAGGATGGACGCGGTATATAGCTTTGTGC‑gggg > 2:934120/1‑148 (MQ=255) aaCTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGG‑GGTTTTAATGCCTGGAGCCGTAGAAGGATGAAAGCGGTATATCGCTTTGCGC‑ggg > 1:788804/1‑148 (MQ=255) aCTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAAt < 1:173324/151‑1 (MQ=255) tCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATACCCGGAGGCGGAGAAGGATGAAAGCGGTTTATAGCTTTTCGC‑GGGgaa > 2:537195/1‑147 (MQ=255) cAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATGCCTGGAGCCGTAGAAGGATGAAAGCGTTATATCGCTTTGCGC‑GGGGAATGTCg > 1:224355/1‑151 (MQ=255) cTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGGGGCGTAGAAGGATGAACGCGTTATATTGCTTTGCGC‑GGGAAATGTCGGTg > 1:884199/1‑150 (MQ=255) gCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCa < 1:405548/151‑1 (MQ=255) cGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATGCCTGGGGCCGGAGAAGGATGAAAGCGGTATATTGCTTTTCGC‑GGGAAATGTTGCTGCGcc > 1:247448/1‑149 (MQ=255) gATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGc < 2:719905/151‑1 (MQ=255) gTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GGTTTTAATGCCTGGGGCCGGAGAAGGGTGAACGCGGTATATTTCTTTTCCC‑GGGAAAAATCGCTGCGCCCGGGGCGtt > 1:877198/1‑151 (MQ=255) tCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGGAGAAGGATGAAAGCGTTATATCGCTTTTCGC‑GGGGAATGTCGCCGCGCCAgggggggtt > 1:882535/1‑144 (MQ=255) taGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATGTCTGGGGCCGTAGAAGGATGAAAGCGGTATATTGCTTTTCGC‑GGGGAAAGTCGCTGCGCCAGGGGCGgttt > 2:337435/1‑148 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATGCCTGGAGCCGGAGAAGGATGAACGCGGTATATCGCTTTTCGC‑GGGGAAAGTCGCTGCGCCAGGGGCGTTTTACCg > 1:122347/1‑148 (MQ=255) aGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGG‑GATTTTAATGCCTGGGGCCGGAGAAGGATGAACGCGGTATATCGCTTTTCGC‑GGGGAATGTCGCTGCGCCCGGGGCGGTTTATCgatg > 1:125008/1‑150 (MQ=255) gTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAg < 2:764653/151‑1 (MQ=255) ttACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAAAGCCTGGTGGCCCAGAAGGATAAACGCGGTATATTGCTTTTCGC‑GGGGAAAGTAGCTGGGCCAGGGGCGGTTTTcccctggac > 1:359968/1‑141 (MQ=255) ccTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGt < 2:867987/150‑1 (MQ=255) tATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GGTTTTAATGCCTGGGGGCGGAGAAGGGTGAACGCGTTATATTGCTTTTCGC‑GGGGAAAGTCGCTGCGCCCGGGGCGGTTTTCCGGTGATCAGCGCcggc‑gg > 1:381476/1‑151 (MQ=255) ggCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATTACCGGAGCCGGGGAAGGGTTAAAGCGGTTTTTAGCTTTTCGC‑GGGAAATGTTGCTTCGCCAGGGGGGTTTTTCCGATGGTCAGCGGCGGC‑ggg > 2:861257/1‑148 (MQ=255) gCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GGTTTTAATGCCTGGAGGCGGAGAAGGATGAAAGCGTTTTATCGCTTTTCGC‑GGGGAATGTCGCTGCGCCCGGGGGGGTTTTCCGATGATCAGCGCCGTC‑GGGCg > 2:330496/1‑149 (MQ=255) gCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GAATTTAATGCCTGGAGCCGTAGAAGGGTGAACGCGGTATATTGCTTTTCGC‑GGGGAAAGTCGCTGCGCCAGGGGCGGTTTTCCGATGATCAGCGCCGTC‑ggggg > 2:156700/1‑147 (MQ=255) gATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTC‑GGGCGTTTg < 2:687459/151‑1 (MQ=255) gATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GAATTTTATTCCCGGAGCCGGAGAAAGGAGAAAGAGGTAGATCGCCTTTCGC‑GGGGAATGTTTCTGGGCCCGGGGCGGTTTTTAGATTAACAGCGCCCTC‑gcggg > 2:536993/1‑143 (MQ=255) tCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GGTTTCAATGCCCGGCGCCGCTGAAGGAAGAAAGCGTTATATCGCCTTTCGCGGGGAAATG‑CGCTGCGCCCGGGGCGGTTTTCCCCTGATCAGCGCCCTC‑GGGCggt > 2:289191/1‑145 (MQ=255) tCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATACCCGGAGCCGGAGAAGGATGAAAGCGGTATATCGCTTTTCGC‑GGGGAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTC‑ggggggtttg > 1:920005/1‑143 (MQ=255) cAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTTCGC‑GGGGAAAGTCGCTGCGCCAGGGGCGGTTTACCGATGATCAGCGCCGTC‑GGGCGttttg > 1:99565/1‑147 (MQ=255) aTAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGG‑GATTTTAATGCCTGGAGGCGTAGAAGGATGAAAGCGGTATATCGCTTTTCGC‑GGGGAATGTCGCTGCGCCCGGGGGGGTTTTCCGATGATTAGCGCCGCC‑GGGCGTGTTGTCg > 2:655440/1‑149 (MQ=255) aTAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATTCCTGGAGCCGCAGAAGGATGAAAGCGGTATATCGCTTTTCGC‑GGGGAATGTCGCTGCGCCAGGGGCGGTTTACCGATGATCAGCGCCGTC‑GGGCGTTTTGTCGcc > 1:651797/1‑151 (MQ=255) tAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGG‑GAATTTAAAGCCCCGAGGCGCAGAAGGATGAAAGCGGTTTATTGCTTTGCGC‑GGGGAATGTCGCTGCGCCAGGGGGGGTTTACCGGTGAACAACGCCGCC‑GGGCGGTTTGGCGccc > 2:28154/1‑151 (MQ=255) tAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGAGGATTCCAAT‑CCCGGGGCCCTGGAAGGAAGAAAGCGGTTTATCGCTTTGCGC‑GGGAAATGTTGCTGCGCCAGGTGCGGTTTTCCGATGATCAGCGCCCTC‑GGGCGGTTTGTCGccc > 1:83460/1‑151 (MQ=255) cTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGGGCCGTAGAAGGATGAAAGCGTTATATCGCTTTTCGC‑GGGGAATGTCGCTGCGCCAGGGGCGTTTTTCCGATGATCAGCGCCGTC‑GGGCGGTTGGTCGCCCGc > 1:145441/1‑150 (MQ=255) caccacAGCGGCCCGGTCATTATGGC‑GGGG‑GAATTCAATGCCCGGAGCCGGAGAAGGAGGAAAGCGGTATATTGCTTTTCGC‑GGGGAAAGTCGCCGCGCCCGGGGCGGTTTTCCGGTGATCAGCGCCGTCGGGGGGTTTGGT‑GCCCGccc > 2:2891/1‑148 (MQ=255) cGGCCCGGTCATTATGGC‑GGGA‑GATTTTAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGGAATGTCGCTGCGCCAGGGGCGGTTTACCGATGATCAGCGCCGTC‑GGGCGTTTGGTCGCCCGCTCGATtttttt > 1:922406/1‑151 (MQ=255) ccGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTC‑GGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCt < 2:185006/151‑1 (MQ=255) ggTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTC‑GGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTAc < 2:326910/151‑1 (MQ=255) c‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTC‑GGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGa > 1:209946/1‑150 (MQ=255) gaTTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTC‑GGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCa < 2:499439/151‑1 (MQ=255) tCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTC‑GGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTg > 2:796727/1‑150 (MQ=255) | CCATTACCTGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTCAGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGC‑GGGA‑GATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGC‑GGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTC‑GGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTG > NC_000913/231548‑231842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |