Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	941,713	0	T	G	47.8%	60.1 / 13.8	46	V252G (GTG→GGG)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (4/20);  new base G (22/0);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.94e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCT  >  NC_000913/941568‑941857                                                                                                                                                  |                                                                                                                                                 tactCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCtt                                                                                                                                             >  2:208292/1‑151 (MQ=255)  actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACt                                                                                                                                              <  1:65205/149‑1 (MQ=255)     cctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACt                                                                                                                                          <  1:972933/150‑1 (MQ=255)                   gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCa                                                                                                                            <  1:527542/150‑1 (MQ=255)                    cGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCTATCTTTaaaa                                                                                                                                >  2:652073/1‑143 (MQ=255)                       aaGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCa                                                                                                                       <  2:972499/151‑1 (MQ=255)                       aaGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCa                                                                                                                       <  2:495334/151‑1 (MQ=255)                          gTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGaa                                                                                                                    <  1:38660/151‑1 (MQ=255)                              gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATc                                                                                                                 <  2:177497/150‑1 (MQ=255)                                aCTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTaa                                                                                                              <  2:298524/151‑1 (MQ=255)                                        tACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCCTTCTATTTTTAAAAAGGACGCCCGaaaatcaaatcccccc                                                                                                      >  2:936191/1‑138 (MQ=255)                                         aCGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCa                                                                                                      <  2:524949/150‑1 (MQ=255)                                              ggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGCGGGGGGACTTACTATCTTGAACAGGAACCCCAGAAAACTAATACCCGCAAGatc                                                                                                 >  2:499158/1‑150 (MQ=255)                                               gcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcat                                                                                               <  2:848457/151‑1 (MQ=255)                                                cTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGCGGGGGGACTTACTATCTTGAACAGGCCCGCCAGAAAACTAATACCCGCATGat                                                                                                  >  1:23085/1‑147 (MQ=255)                                                  gggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcat                                                                                            <  1:716074/151‑1 (MQ=255)                                                   ggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGACTTACTATCTTTAACAGGCACCCCCGAAATCTAATGCCCCCATGatcatc                                                                                              >  1:205513/1‑148 (MQ=255)                                                     cAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGCGGGGGGTATTACTATCTTTAAAAGGCACGCCAAAAATCTAATTACCGCATGAACACCATCg                                                                                          >  2:249791/1‑150 (MQ=255)                                                       gATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATc                                                                                       <  2:553204/151‑1 (MQ=255)                                                       gATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAATCTAAAGCCCGCCTGATCATCATCGATc                                                                                       >  2:702207/1‑151 (MQ=255)                                                        aTGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGATTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATcc                                                                                      >  1:363875/1‑151 (MQ=255)                                                                     gTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGac                                                                         <  2:771036/151‑1 (MQ=255)                                                                       cGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCg                                                                           >  1:696540/1‑147 (MQ=255)                                                                           tatCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAAGCACGCCAGAAATCTAATGCCCCCCTAATCATCATCGATCCGCGCCATACCCACAcc                                                                      >  1:311997/1‑148 (MQ=255)                                                                              cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGc                                                                 <  1:123872/150‑1 (MQ=255)                                                                              cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAATTAATATCTTTAACAAGCAAGCCCGAAATATAATGCCCGCATGATCATCATCGATCCGCCCTATACCGAAAcccggg                                                                  >  2:395852/1‑145 (MQ=255)                                                                                aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAAGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGAACCGCGCTATACCGACACCGGGGcc                                                                >  2:349173/1‑149 (MQ=255)                                                                                   aCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCCGAAAACTAATGCCCGCCTGATCATCATCGATCCGCGCCATACCGACACCGGTGCCGGgcg                                                           >  1:427609/1‑151 (MQ=255)                                                                                      gTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACCCCCGTGCCGGGcgcg                                                         >  2:831796/1‑150 (MQ=255)                                                                                          gCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGa                                                     <  2:692651/150‑1 (MQ=255)                                                                                            tGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATg                                                   >  2:136691/1‑150 (MQ=255)                                                                                            tGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAAGCACGCCCGAAATCTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCCACACCCGGGCCGGGCGCGAAGAt                                                    >  1:960718/1‑149 (MQ=255)                                                                                              gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGt                                                <  1:559962/151‑1 (MQ=255)                                                                                               tAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAAGCACGCCAGAAAACTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATGAGTg                                               >  2:921913/1‑151 (MQ=255)                                                                                                   gCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAAAAGGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATGAGTGGATc                                           >  1:318782/1‑151 (MQ=255)                                                                                                      gTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCt                                        <  2:676358/151‑1 (MQ=255)                                                                                                        ttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGCTTTCTATCTTTTACAAGCCCGCCCGAAATCTTATGCCCGCGAGAACAACAACCGTTCGCGCTATACCGGCACCGGTGCCGGGCGGGAAGGTGAGTGGGTccc                                         >  2:877365/1‑148 (MQ=255)                                                                                                               taaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAAAGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGGTCCCTATTCGTcc                                >  1:739195/1‑150 (MQ=255)                                                                                                                   ccTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTGAACAGGCACGCCCGAAATCTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATGAGGGGATCCCTATTCGTCCggg                             >  2:505012/1‑149 (MQ=255)                                                                                                                         gAAACGCGAATGAGTGGCGGTGGGGGGGCTTACCATCTTGAACAGGCCCGCCAGAAATCTAATGGCCGCATGATCCTCATCGAACCGCCCTATACCGACCCCGGTGGCCGGGGCGAAGAAGAGTGGAGCCCTTTTCCTCCGGGAAc                          >  2:16476/1‑146 (MQ=255)                                                                                                                         gAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAAGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCggggaacgat                      >  2:789240/1‑143 (MQ=255)                                                                                                                         gAAACGCGAATGAGTGGCGGGGGGGGTAATTACTATTTTGAACAAGCACGCCAGAAATATAATGCCCGCATGATCATCATAGATCCGCGCTATACCGACAACGGGGCCGGGGGCGGAGATGAGTGGGTCCCTATTTGGCCGGGGACAGATg                     >  2:628433/1‑151 (MQ=255)                                                                                                                           aaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGcc                   <  1:431810/151‑1 (MQ=255)                                                                                                                             cgcgAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGc                 <  1:208304/151‑1 (MQ=255)                                                                                                                                           ggTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTc   >  2:214452/1‑151 (MQ=255)                                                                                                                                            gTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCt  <  1:454963/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCT  >  NC_000913/941568‑941857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑