Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	993,904	0	A	C	52.1%	65.1 / 81.0	48	G47G (GGT→GGG)	ssuB	aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (18/5);  new base C (0/25);  total (18/30)
Fisher's exact test for biased strand distribution p-value = 4.60e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-08

CCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTG  >  NC_000913/993756‑994050                                                                                                                                                       |                                                                                                                                                   ccGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCaccacc                                                                                                                                                  >  1:529285/1‑151 (MQ=255)  cGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCaccacc                                                                                                                                                  >  2:929752/1‑150 (MQ=255)   gATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGc                                                                                                                                                >  1:529991/1‑151 (MQ=255)    aTTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCt                                                                                                                                               >  1:846328/1‑151 (MQ=255)    aTTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCt                                                                                                                                               <  1:668266/151‑1 (MQ=255)      ttCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTg                                                                                                                                              >  2:626799/1‑150 (MQ=255)         cACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGc                                                                                                                                          >  1:856938/1‑151 (MQ=255)               aGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCccacca                                                                                                                                    <  2:763218/151‑1 (MQ=255)                 cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                  >  1:441017/1‑151 (MQ=255)                  aGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCg                                                                                                                                 >  1:315710/1‑151 (MQ=255)                  aGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCg                                                                                                                                 <  1:437956/151‑1 (MQ=255)                   gACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGc                                                                                                                                >  2:464054/1‑151 (MQ=255)                            ccTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTg                                                                                                                       >  1:841233/1‑151 (MQ=255)                                 accatcatCGGCGTGTTTTCCTGATTTCCG‑GCCGGCGGTGTGGTGCCCGCTACCCCACCGCCGGCGGTTGGCTTTC‑CCAGCCCTGCCAGCGGGCGCAGCGGGGTATTTTTGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTg                                                                                                                  <  2:144730/149‑1 (MQ=255)                                    atcatTCGCGTTTCTTCCTGATTTTCA‑CCCGGGGGTGGGGTCCCCGCAACCACACCGCCTGGGGTTGGCTTTC‑CCAGCCCTGCCGGCAGGCGCAGCAGGGTCTTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGcc                                                                                                                <  1:976355/150‑1 (MQ=255)                                           gcgTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATg                                                                                                        >  2:949409/1‑151 (MQ=255)                                            cgTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                       >  2:225790/1‑151 (MQ=255)                                                        attttCA‑GCCGGGGGTGGGGTCCCCGCAACCACACCGCCGGCGGTTGGCTTTT‑CCAGCCCGGCCAGCGGGCGCAGAAGGGTTCTTTTCCCACCACCGCGGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGtt                                                                                             <  2:489536/147‑1 (MQ=255)                                                        aaTTCCA‑GCCAGGGGTGTGGTGCCCGCTAACACACCCCCTGGGGTTGGCGTTT‑CCAGCCCTGCCAGCGGGCGCAGCGGGGTTTTTTTCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTgg                                                                                           <  1:971694/151‑1 (MQ=255)                                                         aTTTCAGGCCGGGGGGGTGGTCCCCGCAAACACACCCCCTGCGTTTGGCTTTC‑CCGGCCCTCCCCGCGGGCGCAGCGGGGTACTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTgg                                                                                           <  2:26414/151‑1 (MQ=255)                                                           ttCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGtt                                                                                         >  1:45777/1‑150 (MQ=255)                                                            tCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                        >  2:262112/1‑150 (MQ=255)                                                                    ggggTGGGGTGCCCGCTAACCCATCGCTTGCGGTTGGCTTTT‑CCAGACCCGCCAGGGGGCGCAGCGGGTTCTTTTTCCCCCCCCCGTTGCGCCCCCCCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGa                                                                                 <  2:674700/148‑1 (MQ=255)                                                                      gggtgggtcCCCGCAACCCCACCGCCGGCGGTGGGCTTTC‑CCGGCCCTCCCAGCGGGCCCAGCAGGTTCCTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACg                                                                                  <  2:894333/142‑1 (MQ=255)                                                                                 cccaaaCACCTCGCCTGCGGTGGCCTTCC‑CCACCCCTGCCAGCAGGCCCGGCGGGGTTTTTTTCCCACCCCCGCACCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcgcg                                                                      <  2:24696/144‑1 (MQ=255)                                                                                 cGCAAACACACCGCGGGGGGTTGGCTTTC‑CCAGCCCTGCCAGCGGGCGCAGCAGGGTATTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa                                                                   <  1:101697/151‑1 (MQ=255)                                                                                     aaCACATCGCCTGCGTTTGGCGTTC‑CCAGCCCTGCCAGCAGGCGCAGCAGGTTCTTTTTGCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGt                                                                <  1:503157/150‑1 (MQ=255)                                                                                      acacacCGCCGGCGGTGGGTTTTT‑CCAGCCCTGCCAGCGGGCGCAGCGGGGTCCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGttt                                                              <  1:128688/151‑1 (MQ=255)                                                                                         catccccggggttgggCTTTC‑CCAGCCCTGCCAGCGGGCGCAGCAGGTTATTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCt                                                           <  1:225804/137‑1 (MQ=255)                                                                                                  ggggtGGCCTTTCCCAGCCCTGCCAGGAGGCGCAGCGGGTTATTTTTGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTa                                                         <  2:513380/141‑1 (MQ=255)                                                                                                   gtttGGCTTTC‑CCAGCCCTCCCAGCGGGCGCAGCAGGGTTCTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGc                                                     <  1:473161/145‑1 (MQ=255)                                                                                                   gttgcccGTTC‑CCAGCCCCGCCCGCCGCCGCAGCGGTCTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg                                                 <  2:388890/145‑1 (MQ=255)                                                                                                       ggCTTTT‑CCAGCCCTGCCAGCGGGCGCAGCGGGTTCTTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAg                                               <  1:672438/149‑1 (MQ=255)                                                                                                          gTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaac                                           >  2:461612/1‑150 (MQ=255)                                                                                                           ttt‑CCAGCCCTGCCAGCAGGCGCAGCGGGGTACTTTTCCCCCCCCCGCGGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGc                                              <  1:924537/146‑1 (MQ=255)                                                                                                            ttcccaacccgcccAGCGGGCGCAGCGGGTTCCTTTGCCCACCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaaca                                          <  2:857371/138‑1 (MQ=255)                                                                                                            ttcccAGCCCTGCCAGCGGGCGCAGCGGGGTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaac                                           <  1:832812/146‑1 (MQ=255)                                                                                                                   ccccgCCCGCGGGCGCAGCGGGTTTTTTTTCCCCCCACCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTg                                  <  2:562970/147‑1 (MQ=255)                                                                                                                              ggCGCAGCGGGTTTTTTTCCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTc                         <  2:185825/149‑1 (MQ=255)                                                                                                                                   agcagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                    >  1:226547/1‑149 (MQ=255)                                                                                                                                   agcagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                    >  2:886751/1‑149 (MQ=255)                                                                                                                                     cgggttctttttCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                 <  2:280936/142‑1 (MQ=255)                                                                                                                                     cagGGTTCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                 <  1:866587/150‑1 (MQ=255)                                                                                                                                       ggttttttttCCCACCCCCGGTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                 <  2:51115/142‑1 (MQ=255)                                                                                                                                       ggttatttttCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGa                   <  2:139456/140‑1 (MQ=255)                                                                                                                                       gggTACTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGat                  <  1:589350/147‑2 (MQ=255)                                                                                                                                        ggTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGt              >  1:968256/1‑150 (MQ=255)                                                                                                                                          tACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt           >  2:720348/1‑151 (MQ=255)                                                                                                                                             ttttGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCAt        >  1:870183/1‑151 (MQ=255)                                                                                                                                             ttttCCCCCCCCCGCGCCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt           <  2:350473/148‑1 (MQ=255)                                                                                                                                                   caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg  >  1:726361/1‑151 (MQ=255)                                                                                                                                                   caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg  >  1:291039/1‑151 (MQ=255)                                                                                                                                                   caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg  >  2:205447/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                   CCGATTTCCACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTG  >  NC_000913/993756‑994050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑