Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	993,913	0	G	C	51.9%	59.0 / 86.0	52	G44G (GGC→GGG)	ssuB	aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (20/5);  new base C (0/27);  total (20/32)
Fisher's exact test for biased strand distribution p-value = 4.22e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-08

CACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCA  >  NC_000913/993764‑994060                                                                                                                                                        |                                                                                                                                                    cACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGc                                                                                                                                                    >  1:856938/1‑151 (MQ=255)       aGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCccacca                                                                                                                                              <  2:763218/151‑1 (MQ=255)         cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                            >  1:441017/1‑151 (MQ=255)          aGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCg                                                                                                                                           >  1:315710/1‑151 (MQ=255)          aGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCg                                                                                                                                           <  1:437956/151‑1 (MQ=255)           gACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGc                                                                                                                                          >  2:464054/1‑151 (MQ=255)                    ccTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTg                                                                                                                                 >  1:841233/1‑151 (MQ=255)                         accatcatCGGCGTGTTTTCCTGATTTCCG‑GCCGGCGGTGTGGTGCCCGCTACCCCACCGCCGGCGGTTGGCTTTC‑CCAGCCCTGCCAGCGGGCGCAGCGGGGTATTTTTGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTg                                                                                                                            <  2:144730/149‑1 (MQ=255)                            atcatTCGCGTTTCTTCCTGATTTTCA‑CCCGGGGGTGGGGTCCCCGCAACCACACCGCCTGGGGTTGGCTTTC‑CCAGCCCTGCCGGCAGGCGCAGCAGGGTCTTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGcc                                                                                                                          <  1:976355/150‑1 (MQ=255)                                   gcgTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATg                                                                                                                  >  2:949409/1‑151 (MQ=255)                                    cgTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                 >  2:225790/1‑151 (MQ=255)                                                attttCA‑GCCGGGGGTGGGGTCCCCGCAACCACACCGCCGGCGGTTGGCTTTT‑CCAGCCCGGCCAGCGGGCGCAGAAGGGTTCTTTTCCCACCACCGCGGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGtt                                                                                                       <  2:489536/147‑1 (MQ=255)                                                aaTTCCA‑GCCAGGGGTGTGGTGCCCGCTAACACACCCCCTGGGGTTGGCGTTT‑CCAGCCCTGCCAGCGGGCGCAGCGGGGTTTTTTTCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTgg                                                                                                     <  1:971694/151‑1 (MQ=255)                                                 aTTTCAGGCCGGGGGGGTGGTCCCCGCAAACACACCCCCTGCGTTTGGCTTTC‑CCGGCCCTCCCCGCGGGCGCAGCGGGGTACTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTgg                                                                                                     <  2:26414/151‑1 (MQ=255)                                                   ttCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGtt                                                                                                   >  1:45777/1‑150 (MQ=255)                                                    tCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                  >  2:262112/1‑150 (MQ=255)                                                            ggggTGGGGTGCCCGCTAACCCATCGCTTGCGGTTGGCTTTT‑CCAGACCCGCCAGGGGGCGCAGCGGGTTCTTTTTCCCCCCCCCGTTGCGCCCCCCCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGa                                                                                           <  2:674700/148‑1 (MQ=255)                                                              gggtgggtcCCCGCAACCCCACCGCCGGCGGTGGGCTTTC‑CCGGCCCTCCCAGCGGGCCCAGCAGGTTCCTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACg                                                                                            <  2:894333/142‑1 (MQ=255)                                                                         cccaaaCACCTCGCCTGCGGTGGCCTTCC‑CCACCCCTGCCAGCAGGCCCGGCGGGGTTTTTTTCCCACCCCCGCACCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcgcg                                                                                <  2:24696/144‑1 (MQ=255)                                                                         cGCAAACACACCGCGGGGGGTTGGCTTTC‑CCAGCCCTGCCAGCGGGCGCAGCAGGGTATTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa                                                                             <  1:101697/151‑1 (MQ=255)                                                                             aaCACATCGCCTGCGTTTGGCGTTC‑CCAGCCCTGCCAGCAGGCGCAGCAGGTTCTTTTTGCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGt                                                                          <  1:503157/150‑1 (MQ=255)                                                                              acacacCGCCGGCGGTGGGTTTTT‑CCAGCCCTGCCAGCGGGCGCAGCGGGGTCCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGttt                                                                        <  1:128688/151‑1 (MQ=255)                                                                                 catccccggggttgggCTTTC‑CCAGCCCTGCCAGCGGGCGCAGCAGGTTATTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCt                                                                     <  1:225804/137‑1 (MQ=255)                                                                                          ggggtGGCCTTTCCCAGCCCTGCCAGGAGGCGCAGCGGGTTATTTTTGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTa                                                                   <  2:513380/141‑1 (MQ=255)                                                                                           gtttGGCTTTC‑CCAGCCCTCCCAGCGGGCGCAGCAGGGTTCTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGc                                                               <  1:473161/145‑1 (MQ=255)                                                                                           gttgcccGTTC‑CCAGCCCCGCCCGCCGCCGCAGCGGTCTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg                                                           <  2:388890/145‑1 (MQ=255)                                                                                               ggCTTTT‑CCAGCCCTGCCAGCGGGCGCAGCGGGTTCTTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAg                                                         <  1:672438/149‑1 (MQ=255)                                                                                                  gTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaac                                                     >  2:461612/1‑150 (MQ=255)                                                                                                   ttt‑CCAGCCCTGCCAGCAGGCGCAGCGGGGTACTTTTCCCCCCCCCGCGGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGc                                                        <  1:924537/146‑1 (MQ=255)                                                                                                    ttcccaacccgcccAGCGGGCGCAGCGGGTTCCTTTGCCCACCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaaca                                                    <  2:857371/138‑1 (MQ=255)                                                                                                    ttcccAGCCCTGCCAGCGGGCGCAGCGGGGTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaac                                                     <  1:832812/146‑1 (MQ=255)                                                                                                           ccccgCCCGCGGGCGCAGCGGGTTTTTTTTCCCCCCACCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTg                                            <  2:562970/147‑1 (MQ=255)                                                                                                                      ggCGCAGCGGGTTTTTTTCCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTc                                   <  2:185825/149‑1 (MQ=255)                                                                                                                           agcagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                              >  2:886751/1‑149 (MQ=255)                                                                                                                           agcagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                              >  1:226547/1‑149 (MQ=255)                                                                                                                             cgggttctttttCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                           <  2:280936/142‑1 (MQ=255)                                                                                                                             cagGGTTCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                           <  1:866587/150‑1 (MQ=255)                                                                                                                               ggttttttttCCCACCCCCGGTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                           <  2:51115/142‑1 (MQ=255)                                                                                                                               ggttatttttCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGa                             <  2:139456/140‑1 (MQ=255)                                                                                                                               gggTACTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGat                            <  1:589350/147‑2 (MQ=255)                                                                                                                                ggTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGt                        >  1:968256/1‑150 (MQ=255)                                                                                                                                  tACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt                     >  2:720348/1‑151 (MQ=255)                                                                                                                                     ttttGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCAt                  >  1:870183/1‑151 (MQ=255)                                                                                                                                     ttttCCCCCCCCCGCGCCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt                     <  2:350473/148‑1 (MQ=255)                                                                                                                                       tgcccccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATa                 <  1:168472/141‑1 (MQ=255)                                                                                                                                          ccccccccGCTGCGCCCCACCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATa                 <  2:315676/141‑1 (MQ=255)                                                                                                                                           cccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg            <  1:985020/146‑1 (MQ=255)                                                                                                                                           caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg            >  1:291039/1‑151 (MQ=255)                                                                                                                                           caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg            >  1:726361/1‑151 (MQ=255)                                                                                                                                           caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg            >  2:205447/1‑151 (MQ=255)                                                                                                                                             ccccgccggcgcCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg            <  1:694827/141‑1 (MQ=255)                                                                                                                                                ccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctc       >  2:411465/1‑151 (MQ=255)                                                                                                                                                      cGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCa  >  1:2622/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                    CACGGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCA‑GCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTT‑CCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCA  >  NC_000913/993764‑994060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑