Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,9170AC58.8% 38.6 / 68.7 51V43G (GTG→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (19/2);  new base C (0/30);  total (19/32)
Fisher's exact test for biased strand distribution p-value = 4.33e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-09

AGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATG  >  NC_000913/993770‑994064
                                                                                                                                                     |                                                                                                                                                   
aGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCccacca                                                                                                                                                  <  2:763218/151‑1 (MQ=255)
  cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                                >  1:441017/1‑151 (MQ=255)
   aGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCg                                                                                                                                               >  1:315710/1‑151 (MQ=255)
   aGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCg                                                                                                                                               <  1:437956/151‑1 (MQ=255)
    gACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGc                                                                                                                                              >  2:464054/1‑151 (MQ=255)
             ccTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTg                                                                                                                                     >  1:841233/1‑151 (MQ=255)
                  accatcatCGGCGTGTTTTCCTGATTTCCGGCCGGCGGTGTGGTGCCCGCTACCCCACCGCCGGCGGTTGGCTTTCCCAGCCCTGCCAGCGGGCGCAGCGGGGTATTTTTGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTg                                                                                                                                <  2:144730/149‑1 (MQ=255)
                     atcatTCGCGTTTCTTCCTGATTTTCACCCGGGGGTGGGGTCCCCGCAACCACACCGCCTGGGGTTGGCTTTCCCAGCCCTGCCGGCAGGCGCAGCAGGGTCTTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGcc                                                                                                                              <  1:976355/150‑1 (MQ=255)
                            gcgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATg                                                                                                                      >  2:949409/1‑151 (MQ=255)
                             cgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                     >  2:225790/1‑151 (MQ=255)
                                         attttCAGCCGGGGGTGGGGTCCCCGCAACCACACCGCCGGCGGTTGGCTTTTCCAGCCCGGCCAGCGGGCGCAGAAGGGTTCTTTTCCCACCACCGCGGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGtt                                                                                                           <  2:489536/147‑1 (MQ=255)
                                         aaTTCCAGCCAGGGGTGTGGTGCCCGCTAACACACCCCCTGGGGTTGGCGTTTCCAGCCCTGCCAGCGGGCGCAGCGGGGTTTTTTTCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTgg                                                                                                         <  1:971694/151‑1 (MQ=255)
                                          aTTTCAGGCCGGGGGGGTGGTCCCCGCAAACACACCCCCTGCGTTTGGCTTTCCCGGCCCTCCCCGCGGGCGCAGCGGGGTACTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTgg                                                                                                         <  2:26414/151‑1 (MQ=255)
                                            ttCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGtt                                                                                                       >  1:45777/1‑150 (MQ=255)
                                             tCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                      >  2:262112/1‑150 (MQ=255)
                                                     ggggTGGGGTGCCCGCTAACCCATCGCTTGCGGTTGGCTTTTCCAGACCCGCCAGGGGGCGCAGCGGGTTCTTTTTCCCCCCCCCGTTGCGCCCCCCCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGa                                                                                               <  2:674700/148‑1 (MQ=255)
                                                       gggtgggtcCCCGCAACCCCACCGCCGGCGGTGGGCTTTCCCGGCCCTCCCAGCGGGCCCAGCAGGTTCCTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACg                                                                                                <  2:894333/142‑1 (MQ=255)
                                                                  cccaaaCACCTCGCCTGCGGTGGCCTTCCCCACCCCTGCCAGCAGGCCCGGCGGGGTTTTTTTCCCACCCCCGCACCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcgcg                                                                                    <  2:24696/144‑1 (MQ=255)
                                                                  cGCAAACACACCGCGGGGGGTTGGCTTTCCCAGCCCTGCCAGCGGGCGCAGCAGGGTATTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa                                                                                 <  1:101697/151‑1 (MQ=255)
                                                                      aaCACATCGCCTGCGTTTGGCGTTCCCAGCCCTGCCAGCAGGCGCAGCAGGTTCTTTTTGCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGt                                                                              <  1:503157/150‑1 (MQ=255)
                                                                       acacacCGCCGGCGGTGGGTTTTTCCAGCCCTGCCAGCGGGCGCAGCGGGGTCCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGttt                                                                            <  1:128688/151‑1 (MQ=255)
                                                                          catccccggggttgggCTTTCCCAGCCCTGCCAGCGGGCGCAGCAGGTTATTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCt                                                                         <  1:225804/137‑1 (MQ=255)
                                                                                   ggggtGGCCTTTCCCAGCCCTGCCAGGAGGCGCAGCGGGTTATTTTTGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTa                                                                       <  2:513380/141‑1 (MQ=255)
                                                                                    gtttGGCTTTCCCAGCCCTCCCAGCGGGCGCAGCAGGGTTCTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGc                                                                   <  1:473161/145‑1 (MQ=255)
                                                                                    gttgcccGTTCCCAGCCCCGCCCGCCGCCGCAGCGGTCTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg                                                               <  2:388890/145‑1 (MQ=255)
                                                                                        ggCTTTTCCAGCCCTGCCAGCGGGCGCAGCGGGTTCTTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAg                                                             <  1:672438/149‑1 (MQ=255)
                                                                                           gTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaac                                                         >  2:461612/1‑150 (MQ=255)
                                                                                            tttCCAGCCCTGCCAGCAGGCGCAGCGGGGTACTTTTCCCCCCCCCGCGGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGc                                                            <  1:924537/146‑1 (MQ=255)
                                                                                             ttcccaacccgcccAGCGGGCGCAGCGGGTTCCTTTGCCCACCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaaca                                                        <  2:857371/138‑1 (MQ=255)
                                                                                             ttcccAGCCCTGCCAGCGGGCGCAGCGGGGTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaac                                                         <  1:832812/146‑1 (MQ=255)
                                                                                                    ccccgCCCGCGGGCGCAGCGGGTTTTTTTTCCCCCCACCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTg                                                <  2:562970/147‑1 (MQ=255)
                                                                                                               ggCGCAGCGGGTTTTTTTCCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTc                                       <  2:185825/149‑1 (MQ=255)
                                                                                                                    agcagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                                  >  1:226547/1‑149 (MQ=255)
                                                                                                                    agcagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                                  >  2:886751/1‑149 (MQ=255)
                                                                                                                      cgggttctttttCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                               <  2:280936/142‑1 (MQ=255)
                                                                                                                      cagGGTTCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                               <  1:866587/150‑1 (MQ=255)
                                                                                                                        ggttttttttCCCACCCCCGGTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                               <  2:51115/142‑1 (MQ=255)
                                                                                                                        ggttatttttCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGa                                 <  2:139456/140‑1 (MQ=255)
                                                                                                                        gggTACTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGat                                <  1:589350/147‑2 (MQ=255)
                                                                                                                         ggTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGt                            >  1:968256/1‑150 (MQ=255)
                                                                                                                           tACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt                         >  2:720348/1‑151 (MQ=255)
                                                                                                                              ttttGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCAt                      >  1:870183/1‑151 (MQ=255)
                                                                                                                              ttttCCCCCCCCCGCGCCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAtt                         <  2:350473/148‑1 (MQ=255)
                                                                                                                                tgcccccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATa                     <  1:168472/141‑1 (MQ=255)
                                                                                                                                   ccccccccGCTGCGCCCCACCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATa                     <  2:315676/141‑1 (MQ=255)
                                                                                                                                    cccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg                <  1:985020/146‑1 (MQ=255)
                                                                                                                                    caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg                >  2:205447/1‑151 (MQ=255)
                                                                                                                                    caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg                >  1:291039/1‑151 (MQ=255)
                                                                                                                                    caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg                >  1:726361/1‑151 (MQ=255)
                                                                                                                                      ccccgccggcgcCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTg                <  1:694827/141‑1 (MQ=255)
                                                                                                                                         ccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctc           >  2:411465/1‑151 (MQ=255)
                                                                                                                                               cgcccccccACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctcct         <  1:868696/144‑1 (MQ=255)
                                                                                                                                               cgcccccccACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCa      <  2:171906/147‑1 (MQ=255)
                                                                                                                                               cGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCa      >  1:2622/1‑150 (MQ=255)
                                                                                                                                                gcccccccGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCa      <  2:841141/147‑1 (MQ=255)
                                                                                                                                                 cccccccACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctcc          <  2:132878/143‑1 (MQ=255)
                                                                                                                                                  ccccccccCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTc       <  2:151395/146‑1 (MQ=255)
                                                                                                                                                  cccACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATg  >  2:345720/1‑151 (MQ=255)
                                                                                                                                                   ccaccaCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATg  >  2:631984/1‑150 (MQ=255)
                                                                                                                                                   ccaccaCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATg  >  2:413035/1‑150 (MQ=255)
                                                                                                                                                   ccaccaCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATg  >  2:358653/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATG  >  NC_000913/993770‑994064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: