Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	82,594	T→G	E312D (GAA→GAC)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	82,594	0	T	G	100.0%	244.4 / NA	60	E312D (GAA→GAC)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (39/21);  total (39/21)

AGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCT  >  NC_000913/82448‑82736                                                                                                                                                   |                                                                                                                                               aGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGt                                                                                                                                            >  1:714695/1‑151 (MQ=255)   gAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAg                                                                                                                                          <  1:429789/151‑1 (MQ=255)    aaaGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGt                                                                                                                                         <  2:802137/151‑1 (MQ=255)     aaGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGtt                                                                                                                                        <  2:701525/151‑1 (MQ=255)      aGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGttt                                                                                                                                       <  2:41555/151‑1 (MQ=255)        cATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTc                                                                                                                                     <  1:507131/151‑1 (MQ=255)           cGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTcgcg                                                                                                                                  >  1:769192/1‑151 (MQ=255)                  aTTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTc                                                                                                                           >  1:45858/1‑151 (MQ=255)                   ttGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTc                                                                                                                           >  2:622540/1‑150 (MQ=255)                        tAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCATCGCCGCCCGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCAc                                                                                                                      >  2:512776/1‑150 (MQ=255)                        tAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACg                                                                                                                     >  1:748381/1‑151 (MQ=255)                              atatTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATc                                                                                                                <  2:315793/150‑1 (MQ=255)                                atTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCt                                                                                                              <  1:916121/150‑1 (MQ=255)                                atTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTg                                                                                                             >  1:117275/1‑151 (MQ=255)                                  tCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgt                                                                                                           >  2:842212/1‑151 (MQ=255)                                  tCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgt                                                                                                           >  2:299821/1‑151 (MQ=255)                                    aCTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtg                                                                                                          >  1:730498/1‑150 (MQ=255)                                     ctttctttATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGgtgt                                                                                                         >  2:454237/1‑150 (MQ=255)                                      tttctttATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGtat                                                                                                       >  1:317965/1‑151 (MQ=255)                                      tttctttATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGtat                                                                                                       >  2:305879/1‑151 (MQ=255)                                      tttctttATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGtat                                                                                                       >  2:176687/1‑151 (MQ=255)                                       ttctttATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGtat                                                                                                       >  2:339977/1‑150 (MQ=255)                                           ttATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATAc                                                                                                     <  1:252478/148‑1 (MQ=255)                                              taCCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGCTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGATTTTCAGCACGCCATCCTGGTGTATACCggagg                                                                                               >  2:355572/1‑151 (MQ=255)                                               aCCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCggagg                                                                                               >  2:964836/1‑150 (MQ=255)                                                   cATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAgtgt                                                                                          <  1:640174/151‑1 (MQ=255)                                                   cATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAgtgt                                                                                          >  1:691757/1‑151 (MQ=255)                                                   cATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAgtgt                                                                                          <  2:869389/151‑1 (MQ=255)                                                    aTCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTcg                                                                                                                                    <  1:152294/108‑1 (MQ=255)                                                    aTCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTcg                                                                                                                                    >  2:152286/1‑108 (MQ=255)                                                    aTCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGtgtg                                                                                         >  1:230184/1‑151 (MQ=255)                                                      ctcATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCg                                                                                       >  1:442663/1‑151 (MQ=255)                                                        cATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGtgtg                                                                                         <  2:906855/147‑1 (MQ=255)                                                         aTCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAAt                                                                                    >  1:959602/1‑151 (MQ=255)                                                           ccATGCGATGTTTCACCGACGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATgc                                                                                  >  1:73349/1‑151 (MQ=255)                                                             aTGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATgcgc                                                                                >  1:678993/1‑151 (MQ=255)                                                               gCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGcgccg                                                                              >  2:447934/1‑151 (MQ=255)                                                               gCGATGTTGCACCGCCACACGCCCCGACCGAGAGGTCAGATTCGGTTGGATTTGGTTCAGACCAATAGATTCCGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCCCGCGATCCTGGTGTATACCGGAGGAGTGAGCGAATgcgc                                                                                >  2:195878/1‑149 (MQ=255)                                                                           cgccgcACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATg                                                                  <  1:228050/151‑1 (MQ=255)                                                                                   gCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCtttgtt                                                          >  2:880455/1‑151 (MQ=255)                                                                                         aaCGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCgg                                                    >  2:220380/1‑151 (MQ=255)                                                                                            gagaGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGAt                                                 <  1:789541/151‑1 (MQ=255)                                                                                                   cAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCat                                           >  1:972390/1‑150 (MQ=255)                                                                                                    aGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGCAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCatat                                         >  2:86799/1‑151 (MQ=255)                                                                                                      aTTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATAtt                                        >  1:186301/1‑150 (MQ=255)                                                                                                            cTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATc                                 >  2:672326/1‑151 (MQ=255)                                                                                                             tGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCt                                >  1:928790/1‑151 (MQ=255)                                                                                                                aTTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGc                             >  1:424349/1‑151 (MQ=255)                                                                                                                aTTTGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGc                             >  1:642652/1‑151 (MQ=255)                                                                                                                   tGGTTCAGACCAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGcta                           <  2:230169/150‑1 (MQ=255)                                                                                                                             cAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAActggct                <  1:359425/151‑1 (MQ=255)                                                                                                                             cAATAGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAActggct                <  2:367436/151‑1 (MQ=255)                                                                                                                                 aGATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTg            >  1:318827/1‑151 (MQ=255)                                                                                                                                  gATTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTgc           <  1:659888/151‑1 (MQ=255)                                                                                                                                   aTTCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTgcg          <  1:622610/151‑1 (MQ=255)                                                                                                                                     tCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTgcgc         <  2:640171/150‑1 (MQ=255)                                                                                                                                     tCTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGcc        >  2:756658/1‑151 (MQ=255)                                                                                                                                      cTGGTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGcc        >  1:385408/1‑150 (MQ=255)                                                                                                                                        ggTGTCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCata     <  1:396729/151‑1 (MQ=255)                                                                                                                                           gtCATGATGTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATAtct  >  2:635920/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                               AGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTCACCGCCGCACGCCCCGAACGAGAGGTCAGATTCAGCTGGATTTGGTTCAGACCAATAGATTCTGGTGTCATGATTTCGTAGTTTTCGCGGTTTTTCAGCACGCCATCCTGGTGTATACCGGAGGAGTGTGCGAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCT  >  NC_000913/82448‑82736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑