Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,487,1630CT46.5% 50.0 / 13.7 43intergenic (+200/‑72)hrpA/ydcFputative ATP‑dependent helicase/DUF218 superfamily protein, SAM‑binding
Reads supporting (aligned to +/- strand):  ref base C (4/19);  new base T (20/0);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 1.65e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGGCGC  >  NC_000913/1487018‑1487310
                                                                                                                                                   |                                                                                                                                                     
tttATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAATACAATTttct                                                                                                                                                     >  2:547041/1‑148 (MQ=255)
  tattaATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTATGAAAAACGAATTTCATCCtt                                                                                                                                                 >  2:399499/1‑151 (MQ=255)
         tCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGt                                                                                                                                          <  1:430714/150‑1 (MQ=255)
            aaaaCGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGAAAAACGTTTTCCCTCCtttttggagat                                                                                                                                        >  2:466038/1‑142 (MQ=255)
                cGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCt                                                                                                                                  <  1:809747/151‑1 (MQ=255)
                  gTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTtc                                                                                                                                <  1:485533/151‑1 (MQ=255)
                       ggTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAGGACATAAGAATTTCTCCTTTCTTCGTGCTCCGCCTCTCAc                                                                                                                            >  1:431069/1‑150 (MQ=255)
                           aaaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAATAAGATTTTCTCCTTTTTGAGGGGTCCGCTTCTCACtatt                                                                                                                        >  2:847149/1‑150 (MQ=255)
                            aaaTTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACtattat                                                                                                                      <  1:179623/151‑1 (MQ=255)
                                tCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAATAACAATTTCTCCTTTTAGAGTGGTCCCCTTCTCCCtatttttt                                                                                                                    >  1:20394/1‑145 (MQ=255)
                                    gAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCa                                                                                                              <  1:64889/151‑1 (MQ=255)
                                    gAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGACATAAGATTTTCTCCTTTTAGAGGGGTCCGCTTCTCACTATTTTTTCAccc                                                                                                               >  2:383418/1‑148 (MQ=255)
                                      aTTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAACGAATTTCTCCCTTTCGAGGGGTCCCCTTCTCCCtattttttccccc                                                                                                               >  2:910158/1‑139 (MQ=255)
                                       ttCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAAAAAAATTTTTTCCTTTTTGAGTGGTCCGCCTCTCCCtattat                                                                                                                      >  1:823356/1‑140 (MQ=255)
                                        tCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTa                                                                                                           <  2:248460/150‑1 (MQ=255)
                                        tCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTAAGGCTTGCGGAGTGGCGGGTAAGGAAATACGAATTTCTCCCTTTTTAGGGCTACGCTTCTCACtatttttttccgcaggat                                                                                                          >  1:206236/1‑137 (MQ=255)
                                         cAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTAt                                                                                                         <  1:345222/150‑1 (MQ=255)
                                             aaaaTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAGGAAATAAAATTTTCTCCTTTTTGAGTGCTCCGCCTCTCACCATTTTTTCCCCCAGGATtttt                                                                                                     >  2:508974/1‑150 (MQ=255)
                                             aaaaTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAATAAAATTTTCTCCCTTTTGAGTGCTCCCCTTCTCACTATTATTTCACGCacaattttt                                                                                                      >  1:654842/1‑142 (MQ=255)
                                                aTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTGAgg                                                                                                 <  2:795455/151‑1 (MQ=255)
                                                aTACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAgg                                                                                                 <  2:795450/151‑1 (MQ=255)
                                                  aCAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAggg                                                                                                <  1:299511/150‑1 (MQ=255)
                                                            gctgcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAATAAGATTTTCTCCTTTTTTAGGGGTCCGCCTCTCACTATTATTTCACGCCGTATTCTTTAGGGAACGATaa                                                                                        >  1:649426/1‑148 (MQ=255)
                                                              tgcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAACAATTTTCTCCTTTTCGAGGGGTCCGCTTCTCCCTATTATTTCACCCCGTATTTTTTAAGGGACGATAaggagg                                                                                  >  2:287789/1‑151 (MQ=255)
                                                               gcAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGACAAAAAAATTTCTCCTTTTAGAGTGGGCCGCCTCTCCCTCTTTTTTCTCCCCGTATTTTTTAAGGGACGATAaagagg                                                                                  >  1:695331/1‑150 (MQ=255)
                                                                        ataatGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAAAGATTTTCTCCTTTTAGAGTGCTCCCCCTCTCACTATTTTTTCCCCCCGGATTTTTTAAGGGAAGATAAAGAGGGAACATGaa                                                                         >  2:614850/1‑150 (MQ=255)
                                                                             gTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTa                                                                    <  2:586271/150‑1 (MQ=255)
                                                                                     aCAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGttt                                                            <  2:333817/150‑1 (MQ=255)
                                                                                      cAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGAAATACAAATTTCTCCCTTTCTAGGGGGCCGCTTCTCACTATTTTTTCACGGAGTATTTTTTAGGGAAAGATAAGGAGGGAACATGAACATTAACCCCttt                                                            >  1:48280/1‑149 (MQ=255)
                                                                                       aGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGGTTAAGAAATAAGAATTTCTCCCTTCTGAGGGGGCCCCCTCTCTCTATTTTTTCACGCAGAAATTTTAAAGGAAAGAAAAAGAGGAAACATGAAAAATACCCCCttttc                                                          >  2:647620/1‑148 (MQ=255)
                                                                                           tttCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAAGACATAAGAATTTCTCCTTTTATAGGGCTCCGCTTCTCCCTCTTATTTCACCCCGTATTCTTTAGGGAACGATAAAGGGGAAACAAGAAAATTAACCCCCTTTCGAc                                                       >  2:238182/1‑149 (MQ=255)
                                                                                             tCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTGAAAACATAAAAGATTCCCTTTTCCGAGGGGACCGCGTCTTACTATTTTCTCACGCAGGCTTTTTTAGGGGAGGGTAAGGAGGGAACAATAAAAATAACCCGCTTTCCCAccgt                                                    >  1:746499/1‑148 (MQ=255)
                                                                                              ccTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCttt                                                  <  2:758560/151‑1 (MQ=255)
                                                                                                 tAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTTAGGACAAACGAATTTCTTCTTTTTCAGCGGCCCCCCTCTCACTATTATCTCACTCCGCATTTTTTAAGGAAAGATAAAGAGGAAACACAAACACTACCCCCttttccgcgc                                                     >  2:553660/1‑138 (MQ=255)
                                                                                                 tAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCg                                                <  2:20394/150‑1 (MQ=255)
                                                                                                  aaaaGATATGTCAGGCTTGCGGAGTGGCGGTTTAGGAACAAAGATTTTTTCCTTTCAGAGTGCTCCGCTTCTCCCCATTTTATCACGCCGCATTCTTTTAGGGAGGATAAAGAGGGACCATGAACAATACCCCCCTTTCGCCGCTTTTGcc                                              >  1:952326/1‑151 (MQ=255)
                                                                                                            tCAGGCTTGCGGAGTGGCGGTTTAAGAAAAACGAATTTCTCCCTTTAGAGTGCCCCCCCTCTCCCTATTTTTTCCCCCCGGATTTTTTAAGGGAAGAAAAAGAGGAAACAAGAAAAATACCCCCCTTCCGACGCTTTCGcc                                              >  2:911389/1‑141 (MQ=255)
                                                                                                               ggCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGAt                                 <  2:350752/151‑1 (MQ=255)
                                                                                                                   tGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGc                               <  1:584215/149‑1 (MQ=255)
                                                                                                                         gTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATg                       <  1:807195/151‑1 (MQ=255)
                                                                                                                              ggTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATc                  >  1:626282/1‑151 (MQ=255)
                                                                                                                                 tAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCgg                >  2:101299/1‑150 (MQ=255)
                                                                                                                                  aaGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGAc              <  2:67767/151‑1 (MQ=255)
                                                                                                                                   aGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACa             <  1:602214/151‑1 (MQ=255)
                                                                                                                                                 tcctccTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGgcgc  >  2:201473/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAGTGCTCCGCTTCTCACTATTATCTCACGCAGTATTCTTAAGGGAACGATAAGGAGGAACCATGAACATTACCCCGTTTCCGACGCTTTCGCCGGCAACTATAGATGCCATAAATGTTATCGGACAGTGGCTGGCGC  >  NC_000913/1487018‑1487310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: