Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,622,816 | 0 | G | A | 51.8% | 11.0 / 79.9 | 54 | intergenic (+75/+144) | yneM/dgcZ | inner membrane‑associated protein/diguanylate cyclase, zinc‑sensing |
Reads supporting (aligned to +/- strand): ref base G (23/3); new base A (0/28); total (23/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.39e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-05 |
TATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAACT > NC_000913/1622669‑1622966 | tATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGaa < 2:537534/150‑1 (MQ=255) tGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGaaaa < 1:130255/150‑1 (MQ=255) ggCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGaaaaaa < 1:66302/151‑1 (MQ=255) ccGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGaaaaaaa > 2:847764/1‑150 (MQ=255) gTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAAcc > 2:431329/1‑151 (MQ=255) gAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGt > 1:580362/1‑150 (MQ=255) ttttttttttTGTTTTTCGGGCCGTGTATTACAGCCAAAAGGGGGATGAATAATGAAGGGAAATAACCCCTCACCTACCCGCCCCT‑TTG‑TTACAGTTGTGTACAGGGGGCT‑TGT‑TTTTTAG‑GAGGG‑AAAAAAAAAACCGCCAGTAAACcg < 2:353583/150‑1 (MQ=255) tttttttttCTGTTTTTCGGGCCGTTTTTTCCACCCAAAAAGGGGAGAACAAATAAAGGGAAAAAACCCCCCCCCAACCCGCCCCT‑TTT‑TTACGTTTGTGTA‑AAGGGGCCTTGT‑TTTTTAG‑GCCGGAAAAAAAAAAACCGCCAGTAAAcc < 2:547933/150‑1 (MQ=255) tctggttttctggCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGaa > 1:766143/1‑150 (MQ=255) tggttttctggCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGc > 1:701271/1‑151 (MQ=255) ttttctggCCGCTTTTTCCACCCAAAAAGGGGATGATTAATGAACGGAAAAACCCCCTCCCCTACCCGCCCCT‑TTT‑TTACATTTGTGAACAGGGGGCT‑TGT‑TTTTTAG‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAAt < 2:375680/146‑1 (MQ=255) ttttggcccGCTTTTTCCACCCAAAAAGGGGATGACTAAGGAAGGGA‑AAAACCCCCCCCCCAACCCGCCCCTTTT‑TTACGGTTGTGAACAGGGGCCT‑TGT‑TTTTTAT‑GAGGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTg < 1:23413/143‑1 (MQ=255) ttctggCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGc > 2:136365/1‑150 (MQ=255) ctggCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATg > 2:589985/1‑151 (MQ=255) ggCCGCTTATTTCACCCACAAAGGGGATGAAAAATGAAGGGAAATAACCCCCCCCCAACCCGCCCCT‑TTT‑TTACATTTGTGAACAGGGGGCT‑TGT‑TTTTTAG‑GCCGG‑CAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATg < 1:844592/149‑1 (MQ=255) ggCCGCTTATTCCACCCAAAAAGGGGATGACTAATGAACGGAAAAAACCCCCCCCCAACCCGCCCCT‑TTT‑TTACAGTTGTGTACAGGGGGCT‑TGT‑TTTTTAT‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCAt < 2:155699/148‑1 (MQ=255) gcttATTCCACCCAAAAAGGGGATGAAAAATGAAGGGAAATACCCCCTCCCCAACCCGCCCCT‑TTT‑TTACAGTTGTGAACAGGGGGCT‑TGT‑TTTTTAT‑GAGGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAg < 1:50298/147‑1 (MQ=255) gTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAtt > 2:907363/1‑151 (MQ=255) ttttccAGCAAAAAAGGGGAGGACAAAGGAACGGAAATAACCCCTCCCCAACCCGCCCCT‑TTG‑TTACAGTTGTGTAAAGGGGGCT‑TGT‑TTTTTAT‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGa < 2:538134/143‑1 (MQ=255) ttccacccAAAAAGGGGATGACAAATGAGCGGAGATAACCCCCCCCCAACCCGCCCCT‑TTT‑TTACATTTGTGTAAAGGGGGCT‑TGT‑TTTTTAG‑GAGGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTtg < 2:74413/144‑1 (MQ=255) ttacAGCCACAAAGGGTATCACTAAAGAACGGAAATAATCCCTCTCCAACCCCCCCCC‑TTT‑TTAGAGTTGTATAAAGGGGACT‑TTT‑TTTTTAG‑GGCGA‑AAAAAAAAAGCCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTtg < 2:372067/147‑1 (MQ=255) cacccACAAATGGGATAACAAATGACGGGAGAAAACCCCCCCCCT‑ACCGGCCCCTTTG‑TTACAGTTGTGTACAGGGGGCC‑TGA‑TTTTTAT‑GCCGG‑CAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTtgtg < 2:180658/146‑1 (MQ=255) aGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGttt > 1:676391/1‑151 (MQ=255) aGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAAAGCTTGCATGGGTAGATTTGTGctt > 2:253603/1‑151 (MQ=255) ccACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTg > 2:187862/1‑151 (MQ=255) aaaaaTGGGATGACTAATGAGGGGAAATAACCCCTCCCCT‑ACCGGCCCCTTTT‑TTACGGTTGTGAAAAGGGGGCT‑TGT‑TTTTTAT‑GGGGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGtt < 1:904857/144‑1 (MQ=255) aaaTGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCttt > 1:292059/1‑151 (MQ=255) aaaTGGAATGACTAATGAACGGAAATAACCCCCCCCCT‑ACCCGCCCC‑TTGTTTACAGTTGTGAAAAGGGGGCC‑TTA‑TTTTTAT‑GCCGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGttt < 2:577370/145‑1 (MQ=255) aaggaaaaTAACCCCCCCCCAACCCGCCCCT‑TTT‑TTAAAGTGGTGTAAAGGGGGCC‑TGT‑TTTTTAT‑GACGG‑CAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCAtt < 2:607364/143‑1 (MQ=255) aaaacccccccccTACCCGCCCCT‑TTG‑TTACAGTTGTGTACAGGGGGCT‑TGT‑TTTTTAG‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCa < 2:44010/140‑1 (MQ=255) aTAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGc > 2:300230/1‑150 (MQ=255) cccccccTACCCGCCCCT‑TTT‑TTACTGTGGTGAAAAGGGGGCT‑TTT‑TTTTTAG‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGAt < 1:960755/145‑1 (MQ=255) cTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATa > 2:337891/1‑150 (MQ=255) ccTACCCGCCCCT‑TTT‑TTACTGTTGGGAACAGGGGGCT‑TTT‑TTTTTAG‑GGGGG‑CAAAAAAAATCCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACg < 1:376068/149‑1 (MQ=255) acccGCCCCC‑TTT‑TTACGTTTGTTAAAAGGGGGCT‑TGT‑TTTTTAT‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAAt < 1:898248/147‑1 (MQ=255) aaCCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCg > 2:296991/1‑151 (MQ=255) ggCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTg > 2:929908/1‑150 (MQ=255) tG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGt > 1:24486/1‑150 (MQ=255) ttACAGTTGTGTACAGGGGGCT‑TGT‑TTTTTAG‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAg < 1:300705/150‑1 (MQ=255) aaGTTGTGTACAAGGGGCT‑TTT‑TTTTTAT‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATc < 1:667078/150‑1 (MQ=255) aaaGGGGCC‑TTTTTTTTTAG‑GCCGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGtttt < 1:55752/147‑1 (MQ=255) aGGGGCC‑CTA‑TTTTTTTAGGCGG‑CAAAAAAAAGCCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCa < 2:189461/150‑1 (MQ=255) gCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatgaat > 2:910083/1‑151 (MQ=255) cc‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatgaatg > 1:810643/1‑151 (MQ=255) cttGA‑TTTTTAG‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatgaat < 2:940090/148‑1 (MQ=255) cttgttttttAT‑GACGG‑CAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatgaat < 2:889008/145‑1 (MQ=255) tGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGtt > 2:401279/1‑151 (MQ=255) ttttttAT‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGtt < 1:495327/148‑1 (MQ=255) ttttttAG‑GAGGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTa < 1:952314/149‑1 (MQ=255) tttttAT‑GAGGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGt < 2:292086/147‑1 (MQ=255) tttttAT‑GACGG‑AAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTa < 1:596412/149‑1 (MQ=255) ttAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACgg > 2:158888/1‑151 (MQ=255) tAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACgg > 2:480350/1‑150 (MQ=255) aT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACgg > 1:335472/1‑149 (MQ=255) gAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAACt > 2:73953/1‑151 (MQ=255) | TATGGCCGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCC‑TTG‑TTACAGTTGTGTACAAGGGGCC‑TGA‑TTTTTAT‑GACGG‑CGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGGAGCTTAAACT > NC_000913/1622669‑1622966 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |