Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,921,173 | 0 | T | C | 43.9% | 77.3 / 18.6 | 57 | I310V (ATT→GTT) | gudP | putative D‑glucarate transporter |
Reads supporting (aligned to +/- strand): ref base T (28/4); new base C (0/25); total (28/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.72e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACC > NC_000913/2921027‑2921323 | cAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACTCCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATcaca < 1:375550/151‑1 (MQ=255) aGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATcccac > 2:222843/1‑151 (MQ=255) tAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGcac > 2:530952/1‑151 (MQ=255) cGATGATCATCCACTCAACGTTGACGCAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAccgcc < 2:107095/151‑1 (MQ=255) tgatCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGAt > 2:932110/1‑151 (MQ=255) ccACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAaaaccg < 1:387340/151‑1 (MQ=255) aCTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgc > 2:614886/1‑150 (MQ=255) aaCGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCACCATGCCCATTACGACCGGTGTTTTACGCGCAATGTTCAGCGACCCCGTGCGGCGCATCAGCCAGTCGGAAAAAATCCCACCCAGCACACCGCCGATAaaaccgcaaacc < 1:343873/151‑1 (MQ=255) gACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcgg > 1:356055/1‑150 (MQ=255) cGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaac > 2:309917/1‑151 (MQ=255) tAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgg > 1:806022/1‑151 (MQ=255) tAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacg > 1:143710/1‑150 (MQ=255) ttGCAGAACCCCATCCCCACCGACAGCAACATGCCCATTACGATCGGTGTTTTCCGCGAAATGTTCAGCGATCCCGGGCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCcggaacgga < 2:849882/149‑1 (MQ=255) ttGCAAACCCCCATCACCATCGACAGCAACATCCCCATTACGATCGGTGTTTTACGCGCAATTTTCAGCGACCCCGGGCGGCGCATCAGCCAGCCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgg < 2:513593/148‑1 (MQ=255) tggCAAAACCCCACCCCCACCGACAGAAACATGCCCATTCCGACCGGTTTTTTCCGCGAATTTTCCGGCGCCCCCGGGCGGCCCACCAGCCAGCGGAAAATAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAAccgccg < 1:489080/140‑1 (MQ=255) cAGAACCCCATCCCCATCGACAGCAACATGCCCATTACGATCGTTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGcc < 2:823447/150‑1 (MQ=255) aGAACCCCATCCCCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGAAATGTTCAGCGACCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCcac < 2:453403/151‑1 (MQ=255) ccccaccGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTCCAGCGACCCCGTGCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGccc < 2:735797/146‑1 (MQ=255) caccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCt > 2:90942/1‑151 (MQ=255) atcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTc > 2:731341/1‑150 (MQ=255) cGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTc > 2:248311/1‑148 (MQ=255) caacAACATGCCCATTACGATCGGTTTTTTACGCACAATTTCCAGCGATCCCGTGCGGCGCATCACCCAGCCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAAt < 1:603897/147‑1 (MQ=255) aGCAACATGCCCATTACGATGGGTGTTTTCCGCGCAATGTTCAGCGATCCCGTGCGGCGCATCACCCAGTCGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATc < 2:759857/150‑1 (MQ=255) aaCATCCCCATTACGATCGGTTTTTTACGCGCAATGTCCAGCGATCCCGTGCGGCCCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACa < 2:4388/151‑1 (MQ=255) cATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATc > 1:362456/1‑151 (MQ=255) cccTTTACGATGGGTGTTTTCCGCGAAATGTTCAGCGACCCCGTGCGGCGCATCAGCCAGTCGAAAATAATCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgc < 2:730061/151‑1 (MQ=255) cccTTTACGATCGGTGTTTTACGCGCAATGTTCAGCGACCCCGTGCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATc < 1:802907/147‑1 (MQ=255) cccATTACGATCGGTTTTTTACGCGCAATGTTCAGCGACCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgc < 2:831504/151‑1 (MQ=255) ttACGATGGGTTTTTGACGCGAAATGTCCAGCGACCCCGTGCGGCGCACCAGCAAGTGGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGt < 1:89989/149‑1 (MQ=255) cGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGCCATCCCGCGTGCCTg > 1:631712/1‑151 (MQ=255) cGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCt < 2:653075/150‑1 (MQ=255) cGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc > 2:839904/1‑151 (MQ=255) ggTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc > 2:850579/1‑150 (MQ=255) tgtTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc > 1:163727/1‑148 (MQ=255) gtTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc > 2:230486/1‑147 (MQ=255) ttttCCGCCCAATGTCCAGCGACCCGGGGGGGCGCACCAGCCAGCCGAAAAAAACCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGAt < 1:473201/150‑1 (MQ=255) tttACGCGAAATGTCCAGCGACCCCGTGCGGCCCACCAGCCAGTCGGAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATa < 1:449897/150‑1 (MQ=255) cgcgCAATGTCCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCg < 2:325086/151‑1 (MQ=255) gcgcAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCgg > 2:252133/1‑151 (MQ=255) gcgcAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCgg > 2:895052/1‑151 (MQ=255) cAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCggg > 1:282932/1‑149 (MQ=255) gtgccgCGACCCCGGGCGGCGCATCCGCCAGTCGAAAATAACCCCCCCCGCCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAg < 1:577975/146‑1 (MQ=255) gATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaata > 2:936696/1‑150 (MQ=255) tCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaataa > 1:654167/1‑149 (MQ=255) cccGTGCGGCGAACCAGCCATCCGAAAATACCCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaag < 1:33403/150‑1 (MQ=255) gTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagt > 2:808053/1‑151 (MQ=255) tGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagt > 1:55048/1‑150 (MQ=255) gcggcgCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagta > 1:112867/1‑150 (MQ=255) gcggcgAACCACCCAGTCGAAAATAACCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagtaa < 1:269900/151‑1 (MQ=255) cggcgCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagta > 2:776118/1‑149 (MQ=255) gcgcATCAGCCAGGCGGAAATAATCCCACCCAGCACACCGCAGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGAAATAAAGaagta > 1:852197/1‑147 (MQ=255) acaaGCCAGTCGGAAATAACCCCCCCCACCCCCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagtaag < 1:836111/142‑1 (MQ=255) aacaacaaGTCGAAAATATCCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAg < 2:148911/142‑1 (MQ=255) gCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGtt > 1:32166/1‑151 (MQ=255) ccggTCGAAAAAAACCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGc < 1:90941/144‑1 (MQ=255) gccgaaaaTAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGa < 1:580411/144‑1 (MQ=255) aaaTAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACa < 2:786499/148‑1 (MQ=255) tCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGAcc > 2:829175/1‑151 (MQ=255) tCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGAcc > 1:538875/1‑151 (MQ=255) | CAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACC > NC_000913/2921027‑2921323 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |