Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,984,441 | 0 | A | C | 47.5% | 96.7 / 13.2 | 60 | G384G (GGT→GGG) | yqeF | short chain acyltransferase |
Reads supporting (aligned to +/- strand): ref base A (27/4); new base C (0/28); total (27/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.93e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTG > NC_000913/2984292‑2984582 | gAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCacac > 1:38798/1‑151 (MQ=255) aaTATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACAcc < 1:464136/151‑1 (MQ=255) atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACAccc < 1:424029/151‑1 (MQ=255) gTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGTCACACCCTgg < 1:236090/150‑1 (MQ=255) gaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCccgcc > 2:200682/1‑151 (MQ=255) gAGGGAGCCAAAAAAATGTATGTCGCCTTGCCAGCCATCATTATTCAACAGATTAATGCCGGCAGATATTTATCGGTAAGGAGAAAATGCAGAGCTATTCGTCACGTTAAATGGT‑AAATGCCCCCCCCTGGCCCCCGCCGATACAAAGc < 1:12965/149‑1 (MQ=255) gggAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTg > 1:493924/1‑151 (MQ=255) aGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCt > 2:716077/1‑151 (MQ=255) aaaaTGTATGTCGATTTGCCAGCCATCATTATCCAACAGATAAAGGACGGCAAATATTTATCGGTAGGGAGGAAATGCAGAGTTTTTCGTCACGTTCATTGTT‑CATTCCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt < 1:395437/151‑1 (MQ=255) aaaaTGTATGTCGACTTGCCAGCCATAATTATTCAACAAATTAAGGCCGGCAGTTATTAATGGGTAAGGAGAAAATGCAGAGCTATTCGTCACTTTAAATGGT‑CAATCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCttt < 1:711287/151‑1 (MQ=255) aaaTGTATGTCGTCTTGCCAGCCATCATTATTCAACAGATAAATGACGGCAAATATTTAGCGGTAAGGAGAAAATGCAGAGCTTTTCCTCACGTTAAAGGGT‑AAATGCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTg < 1:512008/151‑1 (MQ=255) aaaTGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGAAAGGAGAAAATGCAGAGCTTTTCGTCACGTTAAATGGT‑CAATGCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTg < 2:539064/151‑1 (MQ=255) cGACTTGCCAGCCATCATTATTCAACAGATTAATGCCGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCCCGTTCAATGGT‑CAATGCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTa < 2:623379/150‑1 (MQ=255) cGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAc > 2:340620/1‑151 (MQ=255) gACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg > 2:87403/1‑151 (MQ=255) aCTTGCCACCACTCTTTATAAAACAATTAAAGGATGGCAGTTTTTTATGGGAAAGGAAGAAATGCAGAGCTTTTCGTCGCTTTCAGGGGT‑CAATCCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCa < 1:817449/145‑1 (MQ=255) tGCCAGCCATCATTATTCAAAAGATTAATGACGGCAGATATTTATCGGTAAGGAGAAAATGCAGAGCTATTCGTCACGTTAAATGGT‑CAATGCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGtt < 2:405066/149‑1 (MQ=255) cagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCAcc > 2:817492/1‑151 (MQ=255) catTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCAt > 2:436394/1‑151 (MQ=255) ttattCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg > 2:571910/1‑150 (MQ=255) tattCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGa > 1:52517/1‑150 (MQ=255) aaCAGATTAATGACGGCAGATTTTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACTTTAAATGGT‑CAATGCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAAcc < 2:591551/148‑1 (MQ=255) aaCAAATTAATGGCGGCAGATATTTCTCGGAAAGGAGGAAATGAAGAGTTTTTCGTC‑CGTTTAAAGGTCCAATGCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCaga < 2:755539/151‑1 (MQ=255) aaTGACGGCAGATTTTTATCGGAAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGT‑AAATGCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa < 2:868379/146‑1 (MQ=255) aaTGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTTTTCGTCACGTTCAATGGT‑CAATCCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCa < 1:708457/150‑1 (MQ=255) aaTGACGGCAGATATTTATCGGTAAGGAGAAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAg < 2:151992/151‑1 (MQ=255) cGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTc > 1:290275/1‑151 (MQ=255) ggaaGATTTTTATCGGTAAGGAGAAAATGCAGGGCTATTCGTCCCTTTAAATGGT‑CAATGCCCCCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTc < 2:314462/147‑1 (MQ=255) ggCAGATATTTATGGGAAAGGAGAAAATGCAGAGCTATTCGTCACTTTCAATGGA‑CAAGGCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGa < 1:282754/147‑1 (MQ=255) ggCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTAAATGGT‑CAATGCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCg < 2:75996/151‑1 (MQ=255) atattttttTCGGAAAGGAAAAAATGAAGCGTTATTTGCCGCTTACAAGGAT‑CAATGCCCCGCCCTTTCCCCCGGCGATACAAAGCGTTGCCAGTCCTTTGCTGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAgg < 1:378012/138‑1 (MQ=255) gATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCa > 1:194833/1‑150 (MQ=255) tttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg > 1:889122/1‑151 (MQ=255) tttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg > 1:140771/1‑151 (MQ=255) tttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg > 1:105700/1‑151 (MQ=255) ttATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCgg > 2:742705/1‑150 (MQ=255) tCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgc > 2:896460/1‑151 (MQ=255) ggaaaGGAGAAAATGCAGAGTTATTCGCCCCGTAAAAGGGT‑AAATGCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgcg < 1:644509/147‑1 (MQ=255) aggaggAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTAt > 2:391629/1‑151 (MQ=255) ggagaaAATGCAGAGCTATTCGTCACTTTCAAGGGT‑CAAGGCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATc < 1:266243/151‑1 (MQ=255) ggagaaAATGCAGAGCTATTCGTCACGTTAAATGGT‑CAATGCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCt < 1:911504/148‑1 (MQ=255) aggaAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCgg > 2:103627/1‑151 (MQ=255) aggaAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCgg > 2:306928/1‑151 (MQ=255) cAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCg > 1:70400/1‑150 (MQ=255) agagCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCg > 1:162636/1‑149 (MQ=255) cTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCg > 1:817699/1‑151 (MQ=255) tATTCGTCACGTTAAATGGC‑CAATGCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGc < 1:932340/149‑1 (MQ=255) tATTCGCCACGTTCAATGGT‑AAATGCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGt < 2:144576/147‑1 (MQ=255) aTTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGAt > 2:605678/1‑151 (MQ=255) ttCGTCCCGTTCAATGGT‑AAATCCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGa < 2:665527/149‑1 (MQ=255) ttCGTCACTTTAAATGGT‑AAATGCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGa < 2:930635/149‑1 (MQ=255) ttCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGGGATc > 2:478692/1‑151 (MQ=255) cGTCACGTTAAATGGT‑CAATGCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcg < 1:404467/150‑1 (MQ=255) gTCACGTTCAATGGT‑CAACCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATcgc < 1:493368/150‑1 (MQ=255) aagggT‑CAATCCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcat < 2:44644/146‑1 (MQ=255) aaTGGT‑CAATGCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTAACCGAGTGCGATCGCGCCACcatt < 2:712288/150‑1 (MQ=255) aaTGGT‑AAATGCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcatt < 2:945558/150‑1 (MQ=255) tGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGAGCGATCGCGCCACcattgac > 1:377117/1‑151 (MQ=255) ggT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgac > 2:580693/1‑150 (MQ=255) aaTGCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacattg < 1:72603/150‑1 (MQ=255) aaTGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacattg > 2:562200/1‑150 (MQ=255) | GAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGT‑CAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTG > NC_000913/2984292‑2984582 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |