Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	47.8%	107.3 / 20.4	69	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (33/3);  new base C (0/33);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.57e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACT  >  NC_000913/3443130‑3443426                                                                                                                                                     |                                                                                                                                                      gACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccc                                                                                                                                                    >  1:787988/1‑151 (MQ=255)  aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcccc                                                                                                                                                   <  2:179181/151‑1 (MQ=255)        agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                             >  2:39836/1‑151 (MQ=255)                  aaaacgCGGTGCCCCAGGCTAAAAATACAGCAAATTTGTTGTCAGCCATTTCCAC‑CCAGTCCCCCCCCCGAACCAGGACGCGATGGTCGCCGGAAAAAGAATAATACTGGAAGCGAAGATTGCCGGAATTCCCCCCGCCATATTCa                                                                                                                                        <  1:375999/141‑1 (MQ=255)                     aaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGc                                                                                                                                >  2:729285/1‑151 (MQ=255)                       gCGGTTGCCCAGGCTGAAAATACAGCAAAATTTTTGCCAGCCAGTTCCAA‑CCAGTCCCGCCCCCGAACCATGCCGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCgg                                                                                                                              <  2:788611/151‑1 (MQ=255)                            tGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                         >  2:773924/1‑151 (MQ=255)                             gCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                        >  1:947348/1‑151 (MQ=255)                             gCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                        >  2:84321/1‑151 (MQ=255)                              ccagggttaaaaaTCCAGGAAAATTGTTGTCAGCCAGTTCCAA‑CAAGTACCCCCCCCAAACCATGACGCGATGGTCGCGGGAAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                        <  2:350862/140‑1 (MQ=255)                               ccAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                      >  1:289917/1‑151 (MQ=255)                                cAGGCTAAAAATACAGCAAAATTGTTGTCAGCCAGTCCCAA‑CCAGTACCCCCCCCGAACCATGCCGCGATGGTCGCCGGAAACAAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                         <  2:351210/147‑1 (MQ=255)                                  ggctaaaaaTACAGCAAATTTGTTGTCACCCATTCCCAC‑CCAGTCCCGCCCCCGACCCATGACGCGAGGGTCGCCGGAAACAAAATAATACTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                     <  2:530304/143‑1 (MQ=255)                                  ggCTGAAAATCCAGAGAAATTTTTATCAGCCA‑TTCCAACCCAATACCGCCCCCGAACCATGAGGCGATGGTCGCGGGAAACAAAATAATCTTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                     <  2:35381/149‑1 (MQ=255)                                   gCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                  >  1:477163/1‑151 (MQ=255)                                      gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                               >  1:782310/1‑151 (MQ=255)                                       cAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                              >  2:448256/1‑151 (MQ=255)                                       aaaaTACAGCAAAATTGTTGCCGGCCAGTCCCAA‑CCAGTCCCGCCCCCGAACCAGGCCGCGATGGCCGCGGGGAACAAAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                              <  2:923574/150‑1 (MQ=255)                                          aTACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                            >  2:724751/1‑150 (MQ=255)                                            acagaaaatttgtggTCAGCCAGTTCCAC‑CCAGCCCCCCCCCCAACCCATGACGGGATGGTCGCCGGAAACAAAATAATACGGGAAGGGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                         <  1:644543/142‑1 (MQ=255)                                                 aaaaTTTTTGTCACGCAGTTCCAA‑CCAGTCCCCCCCCCGACCCGTGACGGGGTGGTCGCCGGAAAAAAAAAAATACGGGAAGGGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagca                                                                                                        <  1:795291/146‑1 (MQ=255)                                                  aaaTTGTTGGCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                   >  1:346639/1‑151 (MQ=255)                                                      tgttgtCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                                >  2:835886/1‑150 (MQ=255)                                                        tttccAGCCAGTCCAAA‑CCAGTCCCGCCCCCGAACCATGACGGGAGGGTGGCCGGAAACAAAATAATACGGAAAGCGAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                              <  1:326513/146‑1 (MQ=255)                                                        ttgtCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                             >  1:500008/1‑151 (MQ=255)                                                        ttaccAGCCAGTCCCAC‑CCAGTCCCCCCCCCAAACGATGCCGCGTTGGCCGCCGGAAACAAAATATTACTGGAAGAGAATGTTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                              <  1:741218/146‑1 (MQ=255)                                                        tggtCAGCCAGTCCCAA‑CCAGTCCCCCCCCCAAACAAGGACGCGATGGTCGCCGGGAACAAAATAATACTGGAAGCGAAGATGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                               <  2:135996/147‑1 (MQ=255)                                                             acccggtcccAA‑CCAGTCCCCCCCCCAAACCATGACGCGATGGTCGCCGGGAACAAAATAATACGGAAAGCGAAGATTGCCGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                        <  1:822509/143‑1 (MQ=255)                                                              gCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                                                                       >  2:44249/1‑151 (MQ=255)                                                               ccAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                        >  1:444458/1‑149 (MQ=255)                                                               ccAGTCCCAC‑CCAGTCCCCCCCCCGAACCAGGACGCGATGGTCGCCGGAAACAAAATAATACTGGAAGGGAAGATTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                                                                           <  2:782198/146‑1 (MQ=255)                                                                     ccAC‑CCAGTCCCCCCCCCGAACCAGGCCGCGATGGCCGCGGGAAAAAAAATAATACGGGAAGCGAATACTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                                  <  1:434701/149‑1 (MQ=255)                                                                     ccAA‑CCAGTCCCGCCCCCGAACCATGACGCGATGGTCGCGGGGAACAAAAAAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                                     <  1:834077/146‑1 (MQ=255)                                                                       aa‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                              >  1:880103/1‑151 (MQ=255)                                                                        a‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                             >  1:866174/1‑151 (MQ=255)                                                                            aGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                          >  1:804441/1‑151 (MQ=255)                                                                            aGTACCGCCCCCGAACCATGACGCGATGGTAGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                          >  1:959252/1‑151 (MQ=255)                                                                                ccGCCCCCGAACCAGGCCGCGATGGTCGCCGGGAAAAAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                          <  2:861516/147‑1 (MQ=255)                                                                                     cccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                                 >  1:653565/1‑151 (MQ=255)                                                                                      ccGAACCAGGACGGGATGGTCGCCGGAAAAAAAAAAATACTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACca                                                                <  2:32678/151‑1 (MQ=255)                                                                                               gaggcgATGGTCGCCGGAAAAAAAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                         <  1:789053/146‑1 (MQ=255)                                                                                                  gggATGGTCGCGGGAAACAAAATAATACGGAAAGCAAAGATGGCCGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                     <  1:962713/148‑1 (MQ=255)                                                                                                  gcgATGGTCGCCGGAAACAAAAAAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                                    <  2:191199/151‑1 (MQ=255)                                                                                                    gATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                                    >  1:620688/1‑149 (MQ=255)                                                                                                     aTGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAc                                                 >  2:291167/1‑151 (MQ=255)                                                                                                       ggccGCCGGGAAAAAAATAATACTGGAGGCGAAATTGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                                    <  1:271223/143‑1 (MQ=255)                                                                                                       gctCGCCGGAAACAAAATAAGGCTGAAGGCAAAGATCGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                   <  1:308380/145‑1 (MQ=255)                                                                                                        gtggCCGGGAAAAAAATAATGCGGGAAAAGAAGTTGGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                         <  2:499958/137‑1 (MQ=255)                                                                                                        gTCGCGGGAAAAAAAATAAGTCTGAAAGGAAAGTTTGCGGGAATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                   <  1:768911/146‑1 (MQ=255)                                                                                                         tCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACg                                             >  2:608147/1‑151 (MQ=255)                                                                                                          cgcgGGAAACAAAATAATACGGGAAGCAAAGATTGCGGGTATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                              <  1:681914/149‑1 (MQ=255)                                                                                                          cGCCGGGAAAAAAATAATACTGGAAGCAAATATTGCCGGTATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACg                                             <  1:931222/150‑1 (MQ=255)                                                                                                                 aaCAGAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                       <  2:161162/149‑1 (MQ=255)                                                                                                                    aaaataatACTGGAAGCGAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                   <  1:847289/148‑1 (MQ=255)                                                                                                                     gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                 >  2:850608/1‑151 (MQ=255)                                                                                                                      aataatACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                 >  2:612794/1‑150 (MQ=255)                                                                                                                      aaaaataCGGAAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                                <  2:442632/148‑1 (MQ=255)                                                                                                                       ataataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                                >  1:141843/1‑150 (MQ=255)                                                                                                                       aaaataCTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaa                               <  1:910948/149‑1 (MQ=255)                                                                                                                            acgggAAGAAAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                           <  1:773846/147‑1 (MQ=255)                                                                                                                            aCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                           <  2:80782/150‑1 (MQ=255)                                                                                                                              tGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGa                         >  2:770317/1‑150 (MQ=255)                                                                                                                               ggAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                       >  1:152046/1‑151 (MQ=255)                                                                                                                                 aaGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                     >  2:263294/1‑151 (MQ=255)                                                                                                                                            gCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat           >  2:855641/1‑150 (MQ=255)                                                                                                                                             ccGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac         <  2:451487/151‑1 (MQ=255)                                                                                                                                              cGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact        >  2:528967/1‑151 (MQ=255)                                                                                                                                               gggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatacta       <  2:609331/151‑1 (MQ=255)                                                                                                                                                    tACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaatact  >  2:481880/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACT  >  NC_000913/3443130‑3443426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑