Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,378,8020AC53.3% 30.6 / 41.8 30V48G (GTA→GGA) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
Reads supporting (aligned to +/- strand):  ref base A (11/3);  new base C (0/16);  total (11/19)
Fisher's exact test for biased strand distribution p-value = 6.66e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-05

CCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAA  >  NC_000913/4378655‑4378941
                                                                                                                                                      |                                                                                                                                           
ccTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccg                                                                                                                                          <  1:491933/151‑1 (MQ=255)
   cGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgcca                                                                                                                                       <  1:67969/151‑1 (MQ=255)
    gAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgccac                                                                                                                                      >  1:927445/1‑151 (MQ=255)
     aGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgccacc                                                                                                                                     <  2:686486/151‑1 (MQ=255)
             cGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATAcc                                                                                                                             >  1:580343/1‑151 (MQ=255)
                                  tAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                                                        >  1:349255/1‑151 (MQ=255)
                                   aaaTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                                                        >  1:282063/1‑150 (MQ=255)
                                   aaaTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                                                        >  1:387246/1‑150 (MQ=255)
                                   aaaTGTTTGGCTGACCGACCCTAACTAAAACACCGTTTGCTGTGTGCGGGGCTGTTTTTGGGCGATGCCCGCAAAGCCCAAGGAAAATAAAAAGGTTTTCCCTTGAAAAATTCCCGCCACCGCCATACCCGGGATCTTttgtt                                                                                                               <  2:435771/143‑1 (MQ=255)
                                        ttttGCTGACCGAACCAAACAAAAACACCTTTTGCGGTGGGAGGGGTTTTTTTTGGGCGATCTCCGCATCCCCCAAGGAAAAGTAAAAAGTTTTCCCTTAAAAAATTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAg                                                                                                     <  1:886072/148‑1 (MQ=255)
                                          ttGCTGCCCGACCCTACCTCAAACACCGTTTGCTGTGTGAGGGGCTGTTTTTTGGCGATGTCCGCATACCCCCAGGAAAAGAAATAAGTTTTCCCCTAAAAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCggg                                                                                                 <  2:124948/150‑1 (MQ=255)
                                              tGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAAt                                                                                            >  1:895797/1‑151 (MQ=255)
                                                 ccaacccTAACTAAAACACCGTTTGCTGTGTGACGGGCTGTTTTTGGGCGATGTCCGCATACCCCCAGGAAAAGTAAAAAGTTTTCCCTTAATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATtg                                                                                          <  2:812310/145‑1 (MQ=255)
                                                    acccTAACTAAAACACCGTTTGCTGTGTGAGGGGCTGTTTTTTGGCGATGTCCGCATACCCCCAGTAAAAGAAATAAGGTTTCCCCTGATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCg                                                                                      <  2:681990/149‑1 (MQ=255)
                                                    aaCTTAACTCAAACAACGTTTGCGGTGTGACGGGCTGTTTTTTGGCAATGTCCGCATAGCCCCAGAAAAGTTAAAAGGTTTTCCCTTAAAAATTCCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGc                                                                                       <  1:59602/150‑1 (MQ=255)
                                                      ccTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGAt                                                                                    >  1:409463/1‑151 (MQ=255)
                                                                  aacGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGt                                                                        >  1:168108/1‑151 (MQ=255)
                                                                       ttGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAtt                                                                   >  1:467256/1‑151 (MQ=255)
                                                                         gCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttg                                                                 >  1:439678/1‑151 (MQ=255)
                                                                            gtgtgACGGGTTTTTTTTTGGCGATGTCCGAATGCCCCCAGGAAAAGTAATAGTTTTTCCCCTAATAATTTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttgtt                                                               <  1:310176/150‑1 (MQ=255)
                                                                                gaggggCTTTTTTTGGGCGATGTCCGCATAGCCCCAGGAAAAGTAATAGGTTTCCCCCTGATAAATTACCGCCGCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAt                                                                    <  2:712616/138‑1 (MQ=255)
                                                                                   gggttttttttgGGCGATGCCCGCATACCCCCGGATAAAAAAATAAGTTTTCCCTTAATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAggg                                                          <  2:239787/141‑1 (MQ=255)
                                                                                               ggCTATGTCCGCAGACCCCCAGGAAAAGAAATAAGTTTCCCCTTAAAAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAg                                           <  1:897724/151‑1 (MQ=255)
                                                                                                 cGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGc                                          >  2:946400/1‑150 (MQ=255)
                                                                                                               ccccAGGAAAAGAAAAAGGGTTTCCCCTAATAATTCCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCg                                  <  2:58585/144‑1 (MQ=255)
                                                                                                                ccaaGGAAAAGTAATAGGTTTTCCCTTAAAAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGtaattaa                          <  2:413085/148‑1 (MQ=255)
                                                                                                                       aacataataaGTTTTCCTCTCTTAAATCACAGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAgg                     <  2:132724/145‑1 (MQ=255)
                                                                                                                       aaaGTAAAAAGGTTTCCCCTGAAAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAgg                     <  2:220733/149‑1 (MQ=255)
                                                                                                                               aaGTTTTCCCCTAAAAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGc             <  2:787799/149‑1 (MQ=255)
                                                                                                                               aaGTTTTCCCCTAAAAATTTCCCCCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGcg            <  1:626949/150‑1 (MQ=255)
                                                                                                                                 gttttCCCCTAATAAATTACCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGcgtc          <  2:942455/148‑1 (MQ=255)
                                                                                                                                  gTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGcgtcgt        >  1:613969/1‑151 (MQ=255)
                                                                                                                                   tttCCCCTAAAAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGc             <  1:631459/145‑1 (MQ=255)
                                                                                                                                         caagATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGaa  <  1:934826/148‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                           
CCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAA  >  NC_000913/4378655‑4378941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: