| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,510,395 | 0 | C | A | 49.1% | 56.3 / 39.2 | 57 | S131R (AGC→AGA) | ydcR | putative DNA‑binding transcriptional regulator and putative aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (28/1); new base A (0/28); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAG > NC_000913/1510250‑1510536 | gagaTGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAAc > 2:727576/1‑151 (MQ=255) agaTGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAAcc > 1:398496/1‑151 (MQ=255) atatCAACCCTTATATTTTTTATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTCCCTTTTGCCCCGCCCTTC‑CCC‑GACCCGCGATTTTCCCCCCCCC‑AAC‑AACAAACCCGCTCGCTGGCGCAGGTAACAAAAACCGCCACAGCGATGa < 2:116368/150‑1 (MQ=255) cTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTg > 1:342093/1‑150 (MQ=255) ttATTTTTTTGATTTGTTGCGGGCCAGCCGCGCTCCGTCGGTCGTTCCTTTTCCCCCGCCCTTC‑CCC‑GCCCCGCGTTTTTCCCCCCCCC‑AAC‑AACAAACCCGCTCGCTGGCGCAGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTGa < 2:442376/150‑1 (MQ=255) tttttttGATTTGTGGCAGCCCAGCCGCGATCCGTCGGTCGTCCCGTTTGCCCCGCCCTTT‑CCC‑GCCCCGCGACTTTCCCCCCCCC‑AAC‑AACTAACCCCCTCGCTGGCGAAGGTAAGAAAAACCGCCACAGCGATGAGCGTGATTGaaaa < 2:153035/148‑1 (MQ=255) tttttttGATATGTTGCGGCCCAGCCGCGCCCCGTGGTTCGTCCCGTTTCCCCCGCCCTTC‑CCC‑GCCCCGCGATTTTCCCCCCCCA‑AAA‑AACAAACCCGCTCGCGGGCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGaaa < 2:301336/147‑1 (MQ=255) tttttGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACtt > 2:857059/1‑151 (MQ=255) tttttGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACtt > 2:488396/1‑151 (MQ=255) ttgtttttttGCAGCCCGGCGGCGACCCGTCGGTCGTTCCTTTTCCCCCGGCCTTC‑CCC‑GCCCCGCGATTTTCCCCCCCCA‑AAC‑AACTAACCCGCTCGCTGGCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTAc < 2:794679/142‑1 (MQ=255) ttGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTAcc > 1:314813/1‑151 (MQ=255) tatGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGcc > 1:676949/1‑150 (MQ=255) tGTTGCCGCCCCCTCGCGTTCCGTCGTTCTTTCCTTTTGCCTCGCCTTTC‑CCC‑GCCCCGCGTCTTTCCCCCCCCC‑AAC‑AACTAACCCGCTCCCGGGCGCAGGAAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAg < 2:18539/150‑1 (MQ=255) tGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAgg > 2:474349/1‑151 (MQ=255) tGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAgg > 1:772756/1‑151 (MQ=255) ttGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGa > 1:483081/1‑150 (MQ=255) cAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACg > 1:454948/1‑151 (MQ=255) aGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGc > 1:907595/1‑151 (MQ=255) aGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGc > 1:213374/1‑151 (MQ=255) aGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAAc > 1:50433/1‑151 (MQ=255) cgcgATCCGTCGTTCTTTCCTTTTCCCTCGCCTTTC‑CCC‑CCCCCGCGCCTTTCCCCCCCCC‑AAC‑AACTAACCCCCCCGCTGGCGCAGTTAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAAc < 1:791311/148‑1 (MQ=255) cgcgACCCGTCGGTCTTTCCTTTTGCCTCGGCCTCC‑CCC‑GCCCCGCG‑CTTTTCCCCCCCCAAAC‑AACAAACCCGCTCGCTGGCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAAc < 2:109064/148‑1 (MQ=255) cgACCCGTCGTTCTTCCCTTTCCCCCCGCCCTTC‑CCC‑CCCCCCCGTTTTTCCCCCCCCC‑AAC‑ATCAAACCCGCTCGCGGGCGCAGATAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAAc < 2:76091/146‑1 (MQ=255) gtcggtcgTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCt > 1:681271/1‑151 (MQ=255) ggtcgTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTAtt > 2:344917/1‑151 (MQ=255) tcgTTCCTTTTCCCGCGCCCTTC‑CCC‑CCCCCGCGACTTTCCCCCCCCC‑AAA‑AACAAACCCGCCCGCGGGCGAGGGAAACAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTa < 1:352709/147‑1 (MQ=255) ttCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtcg > 1:360581/1‑151 (MQ=255) cggTTTCCCTCGGCTTTC‑CCC‑GCCCCGCGATTTTCCCCCCCCC‑AAA‑AATAAACCCCCTCGCGGCCGCAGGAAACAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTg < 1:854833/143‑1 (MQ=255) ccttttCCCCCGCCCTTC‑CCC‑CCCCCGCGATTTTCCCCCCCCA‑AAC‑AACAAACCCCCCCGCGGGCGCAGGAAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTAtt < 1:853222/141‑1 (MQ=255) ccttttCCCCGGGCTTT‑CCCGGCCCCGCCATTTTCCCCCCCCC‑AAC‑AACTAACCCGCTCGCTGGCGCGGATAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCtcgt < 2:296809/148‑1 (MQ=255) gTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGc > 2:412144/1‑151 (MQ=255) ccccGGCTTTC‑CCC‑GCCCCGCGATTTTCCCCCCCCC‑AAC‑AACAAAACCGCTCGCGGGCGCAGGAAACAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAAAACTGCGTCAGGCTATTGCTCGTCGCTATg < 1:730873/147‑1 (MQ=255) ccTCGGCCTTC‑CCC‑GCCCCGCGTTTTTCCCCCCCCC‑AAC‑AACAAACCCGCTCGCGGGCGCAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGc < 1:852473/151‑1 (MQ=255) ccgggCCTTTCCCC‑GCCCCGCGCTTTTCCCCCCCCC‑AAC‑AACAAACCCGCGCGCGGGCGCAGAAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGc < 2:201771/148‑1 (MQ=255) ttcccc‑gccccgcGATTTTCCCCCCCCA‑AAA‑AACAAAACCGCCCGCGGGCGCGGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACa < 1:80440/146‑1 (MQ=255) t‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGc > 1:282951/1‑151 (MQ=255) t‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGc > 1:525758/1‑151 (MQ=255) c‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATc > 1:653959/1‑151 (MQ=255) cgACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCAttt > 2:862523/1‑151 (MQ=255) cTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTtctc > 1:191019/1‑151 (MQ=255) tttCCCCCCCC‑AACAAACAAACCCGCTCGCGGGCGAAGGAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCAtt < 2:811263/146‑1 (MQ=255) cccccccCA‑AAA‑AACAAACCCCCCCGCGGGCGAAGAAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCtga < 1:665425/150‑1 (MQ=255) ccccccCA‑AAC‑AACAAACCCGCCCGTGGCCGCAGTAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCtgat < 1:360844/151‑1 (MQ=255) cccccc‑AAC‑AACAAACCCGCCCGCTGCCGCAGGAAAAAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCtgatga < 1:356435/151‑1 (MQ=255) cc‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAAtt > 1:581439/1‑151 (MQ=255) aac‑aacAAACCCGCTCGCGGGCGCGGAAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTg < 2:344504/150‑1 (MQ=255) ac‑aacaAAACCGCTCGTGGGCGCAGGAAAAAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTc < 1:782905/151‑1 (MQ=255) aaaaacAAACCCGCTCGCTGGCGAAGGAAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCCCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTg < 1:810791/147‑1 (MQ=255) aacccgcccgcGGGCGCAGTAAAGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTc < 1:103517/142‑1 (MQ=255) aacccgcccgcGGGCGCAGGAAACAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTa < 1:658290/146‑1 (MQ=255) tcgctGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCgggg > 2:612525/1‑151 (MQ=255) ctGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGt > 2:263676/1‑151 (MQ=255) tGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGtt > 1:119613/1‑151 (MQ=255) gggCGCAGGAAAGAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGc < 2:461679/147‑1 (MQ=255) ggCGCAGAAAAAAAAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTa < 2:631521/151‑1 (MQ=255) gcAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTag > 2:360080/1‑149 (MQ=255) gaaaaGAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTagag < 2:515115/145‑1 (MQ=255) | GAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCC‑GACCCGCGACTTTTCCCCCTCC‑AAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGCAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAG > NC_000913/1510250‑1510536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |