Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA43.2% 39.2 / 14.0 37intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (15/6);  new base A (0/16);  total (15/22)
Fisher's exact test for biased strand distribution p-value = 6.07e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824910‑1825205
                                                                                                                                                   |                                                                                                                                                     
aCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaa                                                                                                                                                   <  1:862559/151‑1 (MQ=255)
aCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                                    >  1:247567/1‑150 (MQ=255)
   ttttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaaga                                                                                                                                                 >  1:592483/1‑150 (MQ=255)
    tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaa                                                                                                                                                >  2:777332/1‑150 (MQ=255)
          cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgcc                                                                                                                                         >  2:325955/1‑151 (MQ=255)
            tatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgccg                                                                                                                                        >  1:432835/1‑150 (MQ=255)
                 aaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                                  >  1:843893/1‑151 (MQ=255)
                    tATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                               >  2:652966/1‑151 (MQ=255)
                     aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                               >  1:476216/1‑150 (MQ=255)
                      ttCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAc                                                                                                                             >  2:80399/1‑151 (MQ=255)
                        ccATACTCGCTCCCGGCCCGTTTTTTTCATTGTTATCGTTCAGGTAACCGTGGATGTTTATCGGCAACTGGGGTGTTTTAC‑GTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCg                                                                                                                           <  1:855471/151‑1 (MQ=255)
                             cTCGCCCCCGGCCCGTTTTTTTCATTTTTATCGTTCAGTTACCCGTGGATGTTTATCGGCAACTCGGGTGTTTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCa                                                                                                                         <  1:624888/148‑1 (MQ=255)
                                 cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                  >  1:656186/1‑151 (MQ=255)
                                   cccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCa                                                                                                                 >  1:57992/1‑150 (MQ=255)
                                            tttttttCATTTTTATCTTTCGGGTAACCGTGGTTGTTTATCGCCAACTCGGGTGTTTTCC‑TTTAGTGGTGATCGGTTTTTTTTCACAACTTTTTAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTa                                                                                                            <  2:720683/143‑1 (MQ=255)
                                            ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                        >  2:11097/1‑150 (MQ=255)
                                                tttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                     >  1:340074/1‑149 (MQ=255)
                                                        ttATCGTTCAGGTACCGGTGGTTGGTTACCGGCAACTGGGGTGATTTAC‑GTTAGTGGTGATCGGTTTTTTTTTACACCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                             <  1:383133/149‑1 (MQ=255)
                                                         tATCGTTCGGGTAACGGGGGATGTTTATCGGCACCTCGGGTGATTTAC‑GTTAGGGGTGATCGGGTTTTTTTTACAATTTTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                           <  2:675441/150‑1 (MQ=255)
                                                              ttaaGGTAACCGTGGATGTTTATCGGCAACTGGGGTGTTTTAC‑TTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                          <  1:812212/143‑1 (MQ=255)
                                                              ttCAGTTACCCTGGGGTGTTTATCGGCAACTGGGTTGATTTAG‑TTTAGTGGTGATCAGGTTTTTTTAACAACTTTTTGAAAAAAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc                                                                                         <  2:101706/147‑1 (MQ=255)
                                                              ttCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGTTTTTTTTACACCTGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt                                                                                        <  2:274255/148‑1 (MQ=255)
                                                                       ccGTGGATGGTTATCGGCAACTGGGGTGTTTTCC‑GTTAGTGGTGATCAGGTTTTTTTTACACCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                            <  1:160744/151‑1 (MQ=255)
                                                                         gTGGATGGTTATCGGCAACTGGGGTGATTTAC‑GTTAGTGGTGATCAGGTTTTTTTTACACCTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt                                                                          <  1:580677/151‑1 (MQ=255)
                                                                         gTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                            >  1:547729/1‑149 (MQ=255)
                                                                                  tATCGGAACTTCGGGGGTTTTAC‑TTTAGTGGTGACCGGGTTTTTTTTACAACTTTTGGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                  <  2:609190/150‑1 (MQ=255)
                                                                                    acagacaactgggTTATTTACGGTTGTTGGTGATCGGGTTTTTTTAACATCTGTTGGGAAAGAAAAAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                  <  2:337915/139‑1 (MQ=255)
                                                                                         aaCTGGGGTATTTTAC‑GTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                            <  2:174638/149‑1 (MQ=255)
                                                                                         aaCTCGGGTGATTTAC‑GTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                              <  1:313578/147‑1 (MQ=255)
                                                                                         aaCTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                           >  2:630623/1‑150 (MQ=255)
                                                                                            tggggTGATTTAC‑GTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                           <  1:487056/145‑1 (MQ=255)
                                                                                              gggTGATTTAC‑GTTAGTGGTGATCGGTTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGt                                                        <  2:27532/148‑1 (MQ=255)
                                                                                              gggTGATTTAC‑GTTAGGGGTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagcag                                                         <  1:204408/147‑1 (MQ=255)
                                                                                               ggTGTTTTCC‑GTTAGGGGTGATCATGTTTTTTTCACACCTGTTTGAGAAAAAAAATGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa                                                    <  1:162472/151‑1 (MQ=255)
                                                                                                        c‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                            >  1:871212/1‑150 (MQ=255)
                                                                                                              gtgttgTTCGGGTTTTTTTTACAACTGTTTGAAAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                      <  2:325547/151‑1 (MQ=255)
                                                                                                              gtggtgATCAGTTTTTTTTTACACTTTTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAcc                                         <  2:790945/148‑1 (MQ=255)
                                                                                                                ggtgAGCAGTTTTTTTTTACACCTGTTTAAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                           <  2:886304/144‑1 (MQ=255)
                                                                                                                        ggCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                             >  1:663283/1‑150 (MQ=255)
                                                                                                                              ttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                      >  1:138521/1‑151 (MQ=255)
                                                                                                                               tttACAACTTTTTGAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                      <  2:277419/150‑1 (MQ=255)
                                                                                                                                         ttggaaaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc               <  2:651339/141‑1 (MQ=255)
                                                                                                                                                  agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt  >  1:752616/1‑151 (MQ=255)
                                                                                                                                                  agaaaaaaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAt   <  2:705018/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824910‑1825205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑