Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,969,794	0	A	C	43.9%	101.7 / 11.5	57	G397G (GGT→GGG)	tap	methyl‑accepting protein IV
Reads supporting (aligned to +/- strand):  ref base A (32/0);  new base C (0/25);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 1.01e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTG  >  NC_000913/1969645‑1969943                                                                                                                                                      |                                                                                                                                                      cgGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCAc                                                                                                                                                      >  1:709900/1‑151 (MQ=255)  gggTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCAcc                                                                                                                                                     >  1:495612/1‑151 (MQ=255)       aCCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGcc                                                                                                                                                 >  2:435339/1‑150 (MQ=255)        ccGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCCCCTGCCAc                                                                                                                                               <  2:350506/151‑1 (MQ=255)           aaCTGACAATATCGATAATGGTCGCGGGGGCGTTATTCACCAGTTCCGACCCCTGCGGGACACGTTTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCGGCCTGCGCGCTACGGCGGGAAAGATTGCGCACTTCCCCTGCCACt                                                                                                                                              <  2:888010/149‑1 (MQ=255)            aCTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTAcc                                                                                                                                           >  2:363593/1‑151 (MQ=255)                 cAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCaa                                                                                                                                       >  2:814025/1‑150 (MQ=255)                  aaTATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCaaa                                                                                                                                      >  1:628747/1‑150 (MQ=255)                  aaTATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCaaa                                                                                                                                      >  2:22212/1‑150 (MQ=255)                        gATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGACCCCTGCTGGACACGATTGACTGACTCTTCGATCACCCCTTTGACCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCa                                                                                                                                  <  2:789739/148‑1 (MQ=255)                              ggTCGCGGCGGCGTTATTCCCCAGTTTCGACCCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTCCGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGc                                                                                                                               <  1:569166/145‑1 (MQ=255)                                 cgcgGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCGGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGctc                                                                                                                       <  1:703411/150‑1 (MQ=255)                                   cggcggcgTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTcc                                                                                                                    >  1:228784/1‑151 (MQ=255)                                          gTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGcgcg                                                                                                              >  1:806153/1‑150 (MQ=255)                                               tCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGc                                                                                                        >  1:372170/1‑151 (MQ=255)                                                    aGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTcc                                                                                                    >  2:660682/1‑150 (MQ=255)                                                      tttCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACt                                                                                                 >  2:370359/1‑151 (MQ=255)                                                      tttCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACt                                                                                                 >  1:378421/1‑151 (MQ=255)                                                       ttCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTg                                                                                                >  2:405161/1‑151 (MQ=255)                                                             cccTGCTGGACACGTTTGACTGACTCTTCGATCAGCCCTTTGATCTTTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCACAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAt                                                                                          <  1:305621/151‑1 (MQ=255)                                                             cccTGCTGGACACGATTGACTGCCTCTTCGATCACCCCTTTGATCTTTTTTGCTGCCTGCGCGCTACGGCGGGCAAGTTTGCGCACTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAt                                                                                          <  2:632484/151‑1 (MQ=255)                                                              ccTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                                                                                         >  1:666689/1‑151 (MQ=255)                                                              ccTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                                                                                         >  2:750823/1‑151 (MQ=255)                                                                 gctgGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGTGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCACTTCCACTGCCGCATTc                                                                                        >  2:361462/1‑149 (MQ=255)                                                                   tgGACACGATTGCCTGCCTCTCCGATCAGCCTTTTGATCTCTTTGGCTCCCTCCGCGCTCCGGCGGGCAAGATGGCCCACTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTc                                                                                        <  2:141731/147‑1 (MQ=255)                                                                        acGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGcc                                                                                  >  2:485037/1‑148 (MQ=255)                                                                            ttGCCTGACTCTCCGACCACCCTTTTGTTCTTTTTTGCTCCCGGCGCGCGCCGGCGGGCAAGTTTCCCCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAata                                                                            <  2:643041/150‑1 (MQ=21)                                                                            ttGACGGCCTCTCCGTTCACCCTTTTGATCTTTTTTCCTCCCGGCGCGCTCCGGCTGGCAAGATTCCGCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAata                                                                            <  1:273177/150‑1 (MQ=21)                                                                               actgactCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTgg                                                                        >  1:815207/1‑151 (MQ=255)                                                                                ctccctctTAGAGCGGCCCTTTACTCTTTTTTGCTCCCCCCGCGCTGGGGCGGGGAAGTTTCCGCACTCCCCCTGCCACTACCGCAAAGCCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATAtt                                                                          <  2:556816/144‑1 (MQ=16)                                                                                 tgactCTTCGATCAGCCTTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTgg                                                                        <  1:132282/149‑1 (MQ=255)                                                                                 tgactCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTc                                                                      >  2:646098/1‑151 (MQ=255)                                                                                    ctctTCCATCACCCTTTTGTTCTTTTTGGCTCCCTCGGCGCTAGGTCTGGCAAGTTTGCCCACTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTg                                                                         <  2:889938/145‑1 (MQ=21)                                                                                     tctTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTgg                                                                   >  1:398817/1‑150 (MQ=255)                                                                                           aTCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCa                                                             >  1:705271/1‑150 (MQ=255)                                                                                                ccttttgtccttttttGCGCCCTGCCCGTTCCGGCGGGAAAGATTCCCCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGc                                                              <  2:173562/132‑1 (MQ=18)                                                                                                 catttGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGt                                                      >  2:861180/3‑151 (MQ=255)                                                                                                            ttttGCTGCCTGCGCGCTAGGGCTGGAAAGTTTGCCCACTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGa                                                   <  2:759547/143‑1 (MQ=37)                                                                                                            ttttGCTGCCTGCGCGCGACGGCGGGAAAGTTTGCGCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATaa                                                <  2:169033/146‑1 (MQ=37)                                                                                                            ttttGCCCCCTGCGCGCTACGGCTGGCAAGATTGCCCCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGc                                             <  1:555650/149‑1 (MQ=255)                                                                                                             tttGCGCCCGGCGCGCTAGGGCTGGCAAGATTGCGCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTg                                           <  1:645079/150‑1 (MQ=37)                                                                                                              ttGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGa                                          >  1:128664/1‑150 (MQ=255)                                                                                                                 ctgcctgcGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAAt                                      >  2:492784/1‑151 (MQ=255)                                                                                                                  tgcctgcGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATg                                     >  1:848405/1‑151 (MQ=255)                                                                                                                    cctgcGCGCTACGGCTGGCAAGATCGCGCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATa                                        <  1:673465/146‑1 (MQ=255)                                                                                                                        cgcgcTCCGGCGGGCAAGTTTGCGCACTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGcc                                <  1:58020/150‑1 (MQ=255)                                                                                                                                ggCTGGAAAGTTTGCCCCCTCCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAAtttttt                        <  1:659907/150‑1 (MQ=25)                                                                                                                                ggCTGGAAAGATTGCGCCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAAtttttt                        <  1:405211/150‑1 (MQ=255)                                                                                                                                 gCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGc                      >  1:389148/1‑151 (MQ=255)                                                                                                                                       agttttGCGCCCTCCCCCTGCCACTACCGCAAACCCTCGCCCCTGCTCTCCGGCGCGGGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGc                  <  1:539033/145‑1 (MQ=25)                                                                                                                                       aaGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCtg                >  1:838761/1‑151 (MQ=255)                                                                                                                                         gATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCtggt              >  1:709379/1‑151 (MQ=255)                                                                                                                                          aTTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCtggtg             >  1:726457/1‑151 (MQ=255)                                                                                                                                              cgcCCTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGa         <  1:95868/151‑1 (MQ=255)                                                                                                                                                cccTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGAtc       <  1:802152/149‑1 (MQ=255)                                                                                                                                                    tCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCt   >  1:713938/1‑151 (MQ=255)                                                                                                                                                     cACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTg  >  1:731225/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CGGGTCACCGAACTGACAATATCGATCATGGTCGCGGCGGCGTTATTCACCAGTTTCGAACCCTGCTGGACACGATTGACTGACTCTTCGATCAGCCCTTTGATCTCTTTTGCTGCCTGCGCGCTACGGCTGGCAAGATTGCGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAATTCCGTCGATAACGCTGATAATGTCGCCAATTTTTTGCGAGCTGGTGGCGATCTCCTG  >  NC_000913/1969645‑1969943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑