Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,676,106	0	C	A	46.0%	54.7 / 32.9	63	L240F (TTG→TTT)	yphD	putative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (23/11);  new base A (0/29);  total (23/40)
Fisher's exact test for biased strand distribution p-value = 3.61e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCG  >  NC_000913/2675957‑2676246                                                                                                                                                      |                                                                                                                                             aGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCcaac                                                                                                                                              <  2:541968/150‑1 (MQ=255)  gCGTAATCCCCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCcaac                                                                                                                                              <  2:472394/149‑1 (MQ=255)    gTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACaaa                                                                                                                                           >  1:592017/1‑150 (MQ=255)               cacaCCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCAcc                                                                                                                               >  1:331952/1‑151 (MQ=255)                    cAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACcgccgc                                                                                                                          >  1:898623/1‑151 (MQ=255)                         aaTGTCCCAAACGGGGACCCGCGCCCGCCGAAAAGGCCCGTCCCGCCGCCCACGCCCGCGGCGATGCCGTCAAACTCCAGACCGTTTGCGGCCCCGGCTTTCCCAGAACCGAGGCGCGCCGCCAAAAAAAGGCCGGTCACCGCCGCtaata                                                                                                                     <  2:335147/151‑1 (MQ=255)                         aaTGTACCGAACAGGGAACCGGGACCGACGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCtaat                                                                                                                      >  2:554839/1‑150 (MQ=255)                         aaTGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCtaat                                                                                                                      >  2:671072/1‑150 (MQ=255)                               cagAACGGGAACCCGCGCCCGCCGGAAAGGCCCGTCCCGCCGCCCCCGCCCGCGGCGATGACGTCAACCCCCAGCCCGTTTGGGGCCCCTGCGTTCCCAGAACCGAGGCGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATccc                                                                                                                <  2:153530/148‑1 (MQ=255)                                  aagggggACCCGCGCCCGCCGAAAAGTCCCGTACCGCCGCCCCCGCCCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCGGCAAAAAAAATGCCGGTCACCGCCGCTAATAATc                                                                                                                  <  2:897860/141‑1 (MQ=255)                                  aaaggggACCCGCGCCCGCGGGAAAGTCCCGTACCGCCGACCACGCCCGGGGCGATGCCGTAAAACCCCAGACCTTTTGCGGCCCCGGCGTTACCAGAACCGGGGCGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCg                                                                                                               <  2:473249/144‑1 (MQ=255)                                  aaCGGGGAACCGCGACCGCCGGAAGGTGCCGTACCGCCGACCCCGACCGGGGCGATGACGTAAAACCCCAGACCTTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGaa                                                                                                             <  1:445791/150‑1 (MQ=255)                                      gggaccgggcaCCGCGGGACAGTGGCGCACCCCCGACCCCGCCCGCGGGAATGACGCCAACCACCGGACCGTTTGCGTCGCTTCCGTCGCCGGAACGGAGCCGGCCCGCAAAAAAAAGGCGGGTCCCCGCCGCTAATAATCCCGAAAggg                                                                                                         <  2:604650/140‑1 (MQ=255)                                      gggACCCGCGCCCGCCGGAAAGTGCCGTACCGCCGACCACGCCCGCGGCGATGACGTCAAACTCCAGCCCGTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAggg                                                                                                         <  1:30389/150‑1 (MQ=255)                                      gggAACCGCGCCCGCCGGAAAGTGCCGTACCGCCGCCCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGa                                                                                                              <  1:333263/145‑1 (MQ=255)                                      ggaacccGCGCCCGCCGAAAAGTGCCGTCCCGCCGCCCACGACCGCGGCGATGACGTCAACCCCCAGACCGTTTGCGGCCCCTGCGTTCCCAGAACCGAGGCGCGCCGCAAACAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAgg                                                                                                          <  2:664880/144‑1 (MQ=255)                                       ggACCCGCGCCCGCCGGAAAGTGCCGCCCCGCCGACCACGACCGCGGCGATGACGTCAAACCCCAGACCGTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCCGCAAAAAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGGt                                                                                                        <  2:478465/150‑1 (MQ=255)                                           ccgggcccgcggAAAAGGGCCGCCCCGCCGCCCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAg                                                                                                    <  2:854060/144‑1 (MQ=255)                                            cgcgACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaa                                                                                                      >  1:480048/1‑147 (MQ=255)                                            cgcgACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaa                                                                                                      >  2:289702/1‑147 (MQ=255)                                             gcgACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGAt                                                                                                  >  2:779738/1‑150 (MQ=255)                                                aCCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATa                                                                                                 >  2:765069/1‑148 (MQ=255)                                                 ccgccgGAAAGTGCCGTACCGCCGACCACGCCCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCCCCGGCGTTACCAGACCCGAGGCGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGAt                                                                                                  <  1:289071/146‑1 (MQ=255)                                                 ccgccgAAAAGTGCCGTCCCGCCGACCACGCCCGCGGCGATGACGTCAAACCCCAGACCGTTTGCGGCACCTGCTTTCCCAGAACCGAGGCGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAg                                                                                               <  1:339787/149‑1 (MQ=255)                                                     cgGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                         >  1:565926/1‑151 (MQ=255)                                                            tGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcg                                                                                   >  1:67539/1‑150 (MQ=255)                                                              ccGTCCCGCCGACCACGCCCGCGGCGAGGCCGCCAAACCCCAGCCCGTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgac                                                                                 <  2:121851/150‑1 (MQ=255)                                                              ccGTACCGCCGACCACCACCGCGGCGATGAGGTCAACCCCCAGCCCGTTTGCGGCTCCTGCGTTCCCAGAACCGAGGCGCGCCGCCACCAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGTTAAAGATAAGAATGCGCACCcga                                                                                  <  1:133533/149‑1 (MQ=255)                                                                   ccgccgACCACGCCCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGACCCGAGGCGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGt                                                                           <  1:847348/151‑1 (MQ=255)                                                                   ccgccgACCACGCCCGCGGCGATGACGCCAAACCCCAGCCCGTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACg                                                                            <  2:644641/150‑1 (MQ=255)                                                                   ccgccgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGt                                                                           >  2:304096/1‑151 (MQ=255)                                                                          ccACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGcc                                                                    <  1:702730/151‑1 (MQ=255)                                                                            aCGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGAAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGc                                                                  >  2:66604/1‑151 (MQ=255)                                                                              gACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcac                                                                >  1:502867/1‑151 (MQ=255)                                                                                ccccggcaATGACTTCAACCTCCAGCCCTTTTCCGCCCCCTCCTTTACCAAACCCGAGCCCCCCCCCCAAAAAAATCCCGTTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcaca                                                               <  1:85619/147‑1 (MQ=255)                                                                                    ggcgATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgc                                                          >  2:20369/1‑151 (MQ=255)                                                                                    ggcgATGACGCCAACCTCCAGCCCGTTTGCGGCACCTCCTTTACCAGAACCGAGGCGCGCCGCCAACAAAAGCCCGTTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgc                                                          <  2:510539/151‑1 (MQ=255)                                                                                       gATGACGTCAACCTCCAGACCGTTTGCGGCACCTGCGTTCCCAGACCCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgcgc                                                        <  1:828603/150‑1 (MQ=255)                                                                                        aTGACGTCAACCTCCAGACCGTTTGCGGCACCTGCGTTCCCAGAACCGAGGCGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGcgcc                                                       <  2:276601/150‑1 (MQ=255)                                                                                        aTGACGTAAAACCCCAGCCCTTTGGCGACACCTGCGTAACAAGAACCGAGCCGCCCCCCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGcgcc                                                       <  2:816657/150‑1 (MQ=255)                                                                                            cGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGt                                                  >  1:329604/1‑151 (MQ=255)                                                                                                          ccGTTGGCGGCCCCTGCGTTACAAGAACCGGGGCGCGCCGCAAAAAAAACGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCaa                                    <  2:134481/151‑1 (MQ=255)                                                                                                          ccGTTCGCGGCCCCTGCGTTCCCAGACCCGAGGCGCGCCCCAAACAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCaa                                    <  1:608220/151‑1 (MQ=255)                                                                                                              ttGCGCCCCCTGCTTTCCCAGACCCGAGGCGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACgg                                 <  1:753715/150‑1 (MQ=255)                                                                                                                acGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCAGGTCACCGCCGCTCATAATCCCGTAAGGGTAAAGATAAGAATGCGCACCAGACGAACGTTGATGACGCACAACTGCGCCGCCGTAGCATTACCGACAACg                                  >  2:326068/2‑147 (MQ=255)                                                                                                                 cGGCACCTGCGTTACCAGAACCGGGGCGCGCCGCAAACAAAATGCCGTTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCa                               <  1:798788/149‑1 (MQ=255)                                                                                                                    ccccTGCGTACCCAGACCGGAGCGCCCCCGCAAAAAAAAGCCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACgg                                 <  2:147265/142‑1 (MQ=255)                                                                                                                     aCCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAAcc                         >  2:769383/1‑151 (MQ=255)                                                                                                                      ccTGCGTTCCCAGACCCGAGGCGCGCCGCCAAAAAAAGGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCg                        <  2:339394/151‑1 (MQ=255)                                                                                                                        tGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCg                        >  2:544535/1‑149 (MQ=255)                                                                                                                         gCGTTCCCAAACCCGGGGCGCCCCGCCAACAAAAGGCCGTTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGa                       <  2:362410/149‑1 (MQ=255)                                                                                                                           gTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgcgc                   >  1:753935/1‑151 (MQ=255)                                                                                                                            ttACCAGACCCGAGGCGCGCCGCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgcgc                   <  1:201549/150‑1 (MQ=255)                                                                                                                             tacaaaagcggAGGCGCCCCCCAAAAAAAAGGCCGTTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgc                     <  1:300323/137‑1 (MQ=255)                                                                                                                               ccAGACCCGAGGCGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCg                <  1:706310/150‑1 (MQ=255)                                                                                                                               ccAAAACCGGGGCGCGCCGCCAACAAAATGCCGCTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGa               <  2:795483/151‑1 (MQ=255)                                                                                                                                 aGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGa               >  1:220248/1‑149 (MQ=255)                                                                                                                                    aCCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCg          >  2:867043/1‑151 (MQ=255)                                                                                                                                     ccGGGGCGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAgg            <  1:426785/148‑1 (MQ=255)                                                                                                                                      cGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCAGAAAGGGGAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCgg         >  2:914346/1‑150 (MQ=255)                                                                                                                                         ggCGCGCCCCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtt       <  1:289739/149‑1 (MQ=255)                                                                                                                                         ggCGCCCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGttt      <  1:749388/150‑1 (MQ=255)                                                                                                                                         ggCGCCCCGCAAAAAAAGTCCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtt       <  1:284632/149‑1 (MQ=255)                                                                                                                                            gcgcCGCCAAAAAAGTCCCGAACTCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCCCAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCg  <  1:408168/151‑1 (MQ=255)                                                                                                                                               ccgcaAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTg    <  1:357349/146‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                             AGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCG  >  NC_000913/2675957‑2676246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑