Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,717,014 | 0 | T | G | 45.6% | 87.6 / 13.3 | 57 | G156G (GGT→GGG) | yiaD | multicopy suppressor of bamB; outer membrane lipoprotein |
Reads supporting (aligned to +/- strand): ref base T (10/21); new base G (26/0); total (36/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.42e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAG > NC_000913/3716867‑3717159 | ccTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGggtggt < 2:422561/151‑1 (MQ=255) ccTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGggtgg < 1:740971/150‑1 (MQ=255) cAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCAc < 1:677710/151‑1 (MQ=255) cAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATg < 1:565346/151‑1 (MQ=255) aaTGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGGTTGGTTATACCGACAGCACGGGGGGGCACGACCCGAACa > 1:125648/1‑148 (MQ=255) aaTGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAAAGTGATTGGTTATAACCACAACACGGGGGGGCACGAACTGaaaa > 1:858969/1‑146 (MQ=255) aTGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTAACGTGATTGGTTATAACGACAGCACGGGGGGGCACGACCTGAACa > 2:852676/1‑147 (MQ=255) cTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAAAGTGATTTGTTATAACGACAGCACGGGGGGGCACGACCTGAACATGCGGCTCTcc > 1:363573/1‑151 (MQ=255) ttCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTTACGTGATTGGTTTTACCCACACCCCCGGGGGGCACCGCCTGAACAAGCGTCTCTccc > 2:918578/1‑151 (MQ=255) ttCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGCTTAAGGGATTGGGTTTTCCCCCAGCACGGGGGGGCCCGACCCGAACATGCGtccctccc > 2:899252/1‑151 (MQ=255) tCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGGTTGGGTATACCGACAGCACGGGGGGGCACGACCTGAACATGCGTCTCTccc > 1:310156/1‑150 (MQ=255) gACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGGGATTGGTTATACCGACAGCACGGGGGGGCACGACCTGAACATGCGGCTCTccc > 1:791109/1‑148 (MQ=255) cagcagcGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCAc < 2:288953/121‑1 (MQ=255) agcagcGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTAACGGGATTGGTTTTACCCACAGCACCGGGGGGCACGCCCCGAAAATGCGCCCCTCCCCGCaa > 2:304230/1‑147 (MQ=255) agcagcGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTAAAGTGATTGGGTATAACGACAGCACGGGGGGGCACGACCTGAACAAGCGTCCCTCCCCGCAACGTg > 2:821972/1‑151 (MQ=255) agcagcGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTAAAGGGGTTGGTTTTAACGACAGCACGGGGGGGCACGACCCGAAAATGCGGCTCTCCCAGCAACggg > 2:181299/1‑149 (MQ=255) cagcGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCg < 2:112920/151‑1 (MQ=255) gACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACATGATTGGTTATACCCACAGCACGGGGGGTCACGACCTGGAAAAGCGTCTCTCCCAGCAACGGGCGGAATcc > 1:645162/1‑151 (MQ=255) ccTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGtt < 1:717240/151‑1 (MQ=255) aaCCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGGGCGGATTCCGTTTcc > 1:145119/1‑149 (MQ=255) ccGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTAACGTGATTGGGTATACCGACAGCACGGGGGGTCACGACCTGGACATGAGTCACTCCCCGCAACGTGCGGATTCCGTTGTCAgc > 1:292742/1‑150 (MQ=255) gcgggcgCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGt < 1:262435/151‑1 (MQ=255) gcgggcgCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGt < 1:7628/151‑1 (MQ=255) gcgggcgCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGGCACGACCCGAAAAAGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAgcg > 2:722216/1‑148 (MQ=255) gggcgCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTg < 2:903997/151‑1 (MQ=255) gggcgCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGGTTCCGTTGCCAGcgcggt > 1:524794/1‑148 (MQ=255) gggcgCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGGGATTGGTTATACCGACAGCACGGGGGGGCACGACCCGAAAAAGCGTCTCTCCCAGCAACGTGCGGAGTTCCTTGCCAGCGCGtt > 2:362866/1‑150 (MQ=255) cgcTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATc < 1:286927/151‑1 (MQ=255) cTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGGTTGGTTATAACGACAGCACGGGTGGTCACGACCTGAAAAAGCGTCTCTCCCAGCAACGCGCGGGTTCCGTTGCCCGCGCGGTGATCAc > 2:875868/1‑151 (MQ=255) cTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAAAGTGATTGGGTATAACGAAAACACGGGGGGTCAAGACCCGAACAAGCGTCTCTCCCAGCAAAGAGCGGGATTCGTTGCCCGCGCGTTTATCa > 2:797046/1‑150 (MQ=255) cccTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAggg < 1:255401/151‑1 (MQ=255) cccTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGGGATTGGGTATACCGACAGCACGGGGGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAggg > 2:342431/1‑151 (MQ=255) ccTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGc < 2:430026/151‑1 (MQ=255) cTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGGGATTGGGTATAACGACAGCACGGGGGGGCACGACCCGAACATGCGTCTCTCCCAGCAACGTGCGGATTTCGTTGCCAGCGCGGTGATCACCCAagg > 2:379355/1‑149 (MQ=255) gACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAACCGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCggg > 1:511316/1‑149 (MQ=255) cGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAAAGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAAAATGCGTCTCTCCCAGCAACGGGGGGGTTCCGTTGCCAGCGCGTTGATCACCCAGGGCgggg > 1:647777/1‑149 (MQ=255) ggCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATAACGACAGCACGGGGGGGCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTgg > 1:125176/1‑148 (MQ=255) gCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGGTATACCGACAGCACGGGGGGTCACGACCTGAAAATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCCGGGCgggg > 1:253186/1‑147 (MQ=255) gTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGcc < 1:901413/150‑1 (MQ=255) ggCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGc < 2:490527/151‑1 (MQ=255) cAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGGTATTGGGTATAACGACAGCACGGGTGGTGACGGCCTGAAAATGCGTCTCTCCCAGCAACGTGCGGATTCCGGTGCCCGCGCGTTGATCACCCAGGGCGTGGACGCCAGcc > 1:686307/1‑150 (MQ=255) tGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGGCACGACCCGAACATGCGTTTCTCCCCGCCACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCCGGGCGTGGAAGCCAGCCCCAt > 1:370650/1‑151 (MQ=255) cTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACCGCACGGGGGGGCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATcc > 1:120177/1‑148 (MQ=255) aaGAGTATCCGAAAACGGCGGTTAACGGGATTGGTTATACCGACAGCACGGGGGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCCGGGCGTGGACGCCAGCCGCATCCGTACTc > 2:217668/1‑150 (MQ=255) ccGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTg < 2:497271/151‑1 (MQ=255) ccGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCCGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCtt > 1:848770/1‑150 (MQ=255) ggTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAAc > 1:176147/1‑151 (MQ=255) aaCGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCa < 2:791006/150‑1 (MQ=255) tGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGc > 1:639759/1‑151 (MQ=255) gATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGcc < 2:524722/151‑1 (MQ=255) gATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGcc < 2:403701/151‑1 (MQ=255) aTTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGcc > 1:640887/1‑150 (MQ=255) ggTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGc < 2:152668/150‑1 (MQ=255) ggTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCa < 1:144805/151‑1 (MQ=255) ccGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCAc > 2:28719/1‑151 (MQ=255) ccGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCAc > 2:90505/1‑151 (MQ=255) cAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGc > 2:425730/1‑150 (MQ=255) cACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGaa < 1:875987/151‑1 (MQ=255) cGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAg < 2:256529/150‑1 (MQ=255) | CCTCAATATGCCGAACAATGTGACCTTCGACAGCAGCAGCGCGACCCTGAAACCGGCGGGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATCACCCAGGGCGTGGACGCCAGCCGCATCCGTACTCAGGGCCTTGGCCCGGCTAACCCAATCGCCAGCAACAGCACCGCAGAAG > NC_000913/3716867‑3717159 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |