| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 718,690 | 0 | T | G | 52.2% | 48.5 / 74.5 | 47 | T591P (ACC→CCC) | speF | ornithine decarboxylase isozyme, inducible |
| Reads supporting (aligned to +/- strand): ref base T (6/16); new base G (24/0); total (31/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.53e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-09 | |||||||||||
TCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTCAGCAGGA > NC_000913/718545‑718839 | tCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATaa < 1:478713/150‑1 (MQ=255) aaaTAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGGTAAAAATCATTCATTTTCTTGCACCACCTACGCAGGGGATAACCgg > 2:284932/1‑145 (MQ=255) aaaTAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAg < 1:751015/150‑1 (MQ=255) gTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATAAAAAACATGCATTTTCTTGCACAACTGACGCAGGGGATAACCGGCGTAGCGctc > 2:701099/1‑147 (MQ=255) aGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCAt < 1:351195/150‑1 (MQ=255) aGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATg < 2:458581/151‑1 (MQ=255) tGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTACAAAACATTCATTTTCTGCCCCAACTGACGCAGGGGATAAACGGCGTAGCGCTCCTCATg > 1:286521/1‑148 (MQ=255) ttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTAAAAAACATGCATTTTCTTACACAACTGACGCAGGGGATAAACCGCGGAGCGCCCTTCATGCT‑Gttttt > 1:646759/1‑147 (MQ=255) tGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATGCATTTCCTTACACAACTGACGCCGGGGATAAACGGCGTAGCGCTCTTCATTCT‑GGTTGt > 1:423875/1‑148 (MQ=255) ggATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACAAATGACGCAGGGGATAACCGGCGGAGCGCTCTTCATGCT‑GTTTTTTGATgg > 1:592021/1‑151 (MQ=255) gATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGa < 2:754535/151‑1 (MQ=255) cATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGt < 1:877407/151‑1 (MQ=255) cATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGt < 2:825094/151‑1 (MQ=255) cTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAATCATGCATTTCCTTCCACAACTGAAGCCGGGGGTAAACCGCCTAGCGCTCCTTCTGCT‑GTTTTTTGATGGAAGGGAg > 1:551825/1‑149 (MQ=255) cgcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCCAAAAAACAAGCGTTTCCTTCCACAAATTACGGCGGGGGTAAACGGCCGAGGGCGCCTTATGCT‑CTTTTGTGATAGAAGGTAGAAca > 2:813094/1‑148 (MQ=255) gcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACAACTGACGCAGGGGATAAACGGCGGAGCGCTCTTCCTGCT‑GTTTGTTGATGGGAGGGAGCAcct > 2:239345/1‑147 (MQ=255) aGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTTCTGACACAACAGACGCCGGGGATAAACCGCGTAGCGCTCTTCATGCT‑GGTTGTAGATGGGAGGGAGCACTTTTGCTTAttg > 1:902549/1‑149 (MQ=255) gTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGgc < 2:201326/151‑1 (MQ=255) gCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTTCACAAATGACGCAGGGGATAAACGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGGAGCACTTCTGCTAATGgcg > 1:87625/1‑150 (MQ=255) tACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTTCTGACACAACTGACGCAGGGGATAAACGGCGGAGCGCTCTTTCTTCT‑GTTTTTAGATGGAAAGGAGGACTTCTTCTAATGGCGCGTCgg > 1:862701/1‑150 (MQ=255) tACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCgg < 1:653299/150‑1 (MQ=255) acggtgcATCTATTTTTGCATTTGAGTCACGTTGTGGAGAGCATACAAGTCATGCAGTTCCTGTCACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTc < 2:633982/141‑1 (MQ=255) aCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCGTAAAAAATATTCATTTTCTTACACCACTGACGCAGGGGATAAACGGCGGAGCGCTCTTCTTGCT‑GTTTTTAGATGGGAGGGAGGACCTCTGCTTATGGGGcggcggg > 2:767964/1‑149 (MQ=255) cGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATAAAAAACATTCATTTTCTTTCCCCACTTACGCAGGGGGTAAACGGCGTAGCGCCCTTCCTGCT‑GTTTTTAGATGGAAGGGAGCACTTTTGCTAATGGGGcggcgggc > 2:695120/1‑148 (MQ=255) ggAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATTCATTTCCTTACACAACTGACGCAGGGGATAAACCGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGGAGGTAGGACTTCTGCTAATGGCGCGTCGGGCTc > 1:201988/1‑151 (MQ=255) cATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATTATTCTTTTTCTGACACAAATGACGCAGGGGGTAAACGGCGGAGCGCTCTTCCTGCT‑GTTTGTAGATGGAAGGGAGCACTTCTTCTAATGGCGCGGCGGACTCaa > 2:826543/1‑149 (MQ=255) cATCTCTTTTAGCAGTTGTTTCACGTTGTGGCGGGGCAACAAAACATGCATTTTCTTAAACAAATGAAGCAGGGGTTAAACCGCGTAGCGGCCTTCTTGAT‑GTTTTTTGAAGGGAAGGAGGACTTCTTCTTATGGCGGGGCGGCGTCaa > 1:828752/1‑149 (MQ=255) ctctTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTACAAAACATGCTTTTTCTTACCCAACCCACGCCGGGGATAAACAGCGGAGCGCTCTTCCTGCT‑CTTTGTAGATGGAAGGGAGCACCTCTGCCCATGGGGCGTCgg > 2:464286/1‑140 (MQ=255) ctTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGt < 2:547818/149‑1 (MQ=255) tttGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATTGATTTTCTTACACCACTGACGCAGGGGATAACCCGCGGAGCGCTCTTTATTCT‑GTTTGTAGGTGGAAGGGAGGACTTCTGCTTATTGCGCGCCGGGCTTCAGCAGgttttt > 2:760422/1‑150 (MQ=255) tGCAGTTGTTTCACGTTGTGGCGGGGATAAAAAACATGCATTTTCTGAAACAACAGCCGGAAGGGAAAAACCGCGTCGGGCTCTTTATGCTGGGTTGTGGA‑GGAAGGGAGGACTTCTGCTAAAGGCGCGTCGGGCTCAAGCAGgtttttg > 1:152227/1‑148 (MQ=255) gttgttTCACGTTGTGGCGGGCCTACAAAACATGCATTTTCTTACACAACTGACGCAGGGGATAAACGGCGGAGCGCTCTTCATTCT‑GTTTTTAGATGGGAGGGAGCACTTCTTCTAATGGCGCGTCGGGCTCAAGCAGGTTTTTGAAGCg > 2:13036/1‑151 (MQ=255) gttgttTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTGAAAAAAATAACGGCGGGGGTAAACGGCGGAGCACTCTTCTTGCT‑GTTTTTAGATGGGAGGGAGCACCTTTTCTTATGGGGCGTCGGACTCACACAGTTTTTTGAAGc > 1:228654/1‑150 (MQ=255) gttTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTa < 2:288756/150‑1 (MQ=255) ttCACGTTGTGGCGGGCATACAAAAAAATCATTTTCTGACACAAATGACGCAGGGGATAAACGGCGCAGCGCCCTTTATGCT‑GTTTTTAGATGGAAGGGAGCACTTCTGCTAATGGCGCGTCGGGCTCAAGCAGGTTTTTGAAGGGTAcc > 1:605497/1‑150 (MQ=255) aCGTTGTGGCGGGCATACAAATAATGCATTTTCTTACACAACTTACGCCGGGGATAAACCGCGTAGCGCCCTTTCTTCT‑GTTTTTTGATTGAAGGGAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTTTAAGCGTAAcagc > 2:728294/1‑150 (MQ=255) cGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGcaaca > 2:639069/1‑150 (MQ=255) gggCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGcaaca > 2:728218/1‑149 (MQ=255) aCAAATCATGCATTTCCTGACACAACTGACGCAGGGAATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTg > 2:384425/1‑151 (MQ=255) gCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTgg < 2:553449/150‑1 (MQ=255) gCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGc > 1:708916/1‑151 (MQ=255) ccTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATAtc > 2:586470/1‑151 (MQ=255) cTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATAtct > 1:673654/1‑151 (MQ=255) acacAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATAtcttct < 1:10553/151‑1 (MQ=255) cacaACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTg < 2:525207/151‑1 (MQ=255) gACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGt > 2:640777/1‑151 (MQ=255) gggTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTcagcag < 2:299846/151‑1 (MQ=255) tataACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTCAGCAGGa > 2:438653/1‑150 (MQ=255) | TCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCT‑GTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTCAGCAGGA > NC_000913/718545‑718839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |