Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,079,4680AG43.6% 80.1 / 12.5 55D55G (GAT→GGT) putPproline:sodium symporter
Reads supporting (aligned to +/- strand):  ref base A (2/29);  new base G (24/0);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.31e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAAC  >  NC_000913/1079321‑1079616
                                                                                                                                                   |                                                                                                                                                         
cGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGatat                                                                                                                                                        <  1:915190/151‑1 (MQ=255)
 gATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATg                                                                                                                                                       <  1:520450/151‑1 (MQ=255)
  aTGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGa                                                                                                                                                      <  2:204911/151‑1 (MQ=255)
    gTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATg                                                                                                                                                       >  1:752197/1‑148 (MQ=255)
      tGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCg                                                                                                                                                   <  2:919848/150‑1 (MQ=255)
      tGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCg                                                                                                                                                   <  1:684619/150‑1 (MQ=255)
        gTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCggct                                                                                                                                                <  1:411552/151‑1 (MQ=255)
        gTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCggct                                                                                                                                                <  1:922944/151‑1 (MQ=255)
             aTTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGgctggctg                                                                                                                                           <  2:693482/151‑1 (MQ=255)
                ttGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGtt                                                                                                                                        <  2:283353/150‑1 (MQ=255)
                ttGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGCGgctgggtggt                                                                                                                                         >  2:362439/1‑148 (MQ=255)
                     tCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATgg                                                                                                                                   <  1:17759/150‑1 (MQ=255)
                         tatCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGtt                                                                                                                               <  2:771120/150‑1 (MQ=255)
                              ttGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTTTGTACGGCTGGCTGGTTATGGGGGTGGCggg                                                                                                                        >  2:914067/1‑151 (MQ=255)
                                  cATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGCGGCTGGGTGTTAATGGGGGTGGCggg                                                                                                                        >  1:627463/1‑147 (MQ=255)
                                    tGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGGGTGGGTATGAGGGGGCGGCCGGTTATGGGGGTGGCggg                                                                                                                        >  1:342839/1‑145 (MQ=255)
                                     gATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGt                                                                                                                 <  1:317521/150‑1 (MQ=255)
                                     gATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGGGGGTGGGTGTTTATGGGGGTGGCGGGCGGTGtt                                                                                                                >  1:310014/1‑151 (MQ=255)
                                           gattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATTAGGGGGGGGGGGGTTATGGGGGGGGCGGGGGCGGtttttttt                                                                                                          >  2:150454/1‑151 (MQ=255)
                                            attgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTtttcttt                                                                                                         <  2:663279/151‑1 (MQ=255)
                                                ggTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGGCGGGGACTAGCGGGTGGCTGTTTAATGGGTTGCGCGGGGCTGCtttttttt                                                                                                         >  1:229830/1‑147 (MQ=255)
                                                   ttATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATGGGCGGGTGGGTGGTAATTGGGGTGGCGGGGGGTGTTTTTTTTTTCCgggt                                                                                                   >  1:601840/1‑150 (MQ=255)
                                                        gCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCg                                                                                             <  2:473754/151‑1 (MQ=255)
                                                          cTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGGAATTGGGGGGGGGGTGGTTATTGGGTTTTCGGGGGCCGTTTTTCTTTTCGCGGttttc                                                                                              >  1:690985/1‑146 (MQ=255)
                                                               gATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTg                                                                                      <  1:702269/151‑1 (MQ=255)
                                                                aTCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGAGATTAGCGGCGGGCTGTTATTGGGGTTGTCGGGCGGTGTTTTTTTTTTCgggggtttctcaagatgg                                                                                     >  2:663305/1‑136 (MQ=255)
                                                                     cGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGGTGGGCGTTTATTGGGGTGGCGGGGGGTGGTTTTTTTTTTGGGGTTTTCGGAAGCTgg                                                                                     >  2:242873/1‑146 (MQ=255)
                                                                      gAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGGGGGTGGGTGTTTATGGGGGTGCCGGGGGCTGTTTTTTTTTTCGGGGTTTTCGAAAGCTGGATCGcc                                                                               >  1:860638/1‑151 (MQ=255)
                                                                       aaaaaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATCTGAGGGGGGGTGTTTAAAGGGGTGCCGGGGGCTGGTTTTTTTTTCGGGGTTTTCCACAGCttggttgcc                                                                               >  1:491924/1‑142 (MQ=255)
                                                                       aaaaaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGGATGAGGGGGTGGCTGTTTATGGGGGGGCCGGGGGGCGTTTTTTTTTTCCGGGTTTTCCACAAGTGGGTCGccc                                                                              >  2:218843/1‑150 (MQ=255)
                                                                                gacgacTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATGAGCGGCTGGGTGTTTATGGGGGTGCCGGGGGCCGGTTTTTTTTTCGGGGTTTCCGAAAGCTGGATTGCCAtt                                                                           >  1:560491/1‑144 (MQ=255)
                                                                                   gacTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACAt                                                                <  1:663390/151‑1 (MQ=255)
                                                                                    acTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGGTATGAGCGGCGGGGTGTTTATGGGGGTGGCGGGGGCTGTTTTTTTTTTCGGGGTTTTCGGAAGCTGGGTCGCCATTGGCCTGACaat                                                               >  1:426753/1‑149 (MQ=255)
                                                                                         atTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGgc                                                           <  1:768962/150‑1 (MQ=255)
                                                                                            cTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGCGGGCGGGTGTTTATTGGGGTGGCGGGGGCTGGTTTTTTTTCCTGGGTTTTTGCAAGAGGGATCGCCATTTGGCTGGCATTAGGggcg                                                        >  2:806293/1‑150 (MQ=255)
                                                                                             tGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATGAGGGGCGGGGGGGTAATGGGGGTGGCGGGGGGGGGTTTTTTTTTCGGGGTTTTTGAAAACTGGAACGCCATTTGCCTGGCATTAgggggggg                                                      >  2:644716/1‑145 (MQ=255)
                                                                                                gCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGAt                                                   <  1:808518/151‑1 (MQ=255)
                                                                                                  ggTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGGGGGTATGAGGGGCGGGGTGGTAATTGGGGTGGCGGGGGCTGTTTTTTTTTTTTGGGTTTTCGAAAACTGGGTCGCCCCTGGCCTGGCAttttgggcggggggt                                                  >  2:258925/1‑137 (MQ=255)
                                                                                                    tCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATGGGGGGCTGGGTGGTGATATGGGTGGCGGGGGCTGGGTTTTTTTTCGCGAGTTTCGGGAGCTGGAGCGCCGTTGGCCTCACCTTattggcgtg                                                      >  2:270534/1‑136 (MQ=255)
                                                                                                        aGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTgg                                            <  2:65215/150‑1 (MQ=255)
                                                                                                         gTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGaa                                          <  2:488100/151‑1 (MQ=255)
                                                                                                           cTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGGACGGGCGGGTGTTTATTGGGGTGGCCGGGGCCGGTTTTTTTTTCGGGGTTTTCGGAAGCTGGGTCGCCATTGGCCCTGCCTTTTGGGCGGGGGTTAACTGGAAg                                         >  2:799345/1‑150 (MQ=255)
                                                                                                           cTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGCGGGGGGGTGGTAATGGGGGTGGCGGGGGGGGTTTTTTTTTTCGGGGTTTTCCGAAACTGGATCGGCCTTGGCCTGACAATTGGCGCGGGGGTTAAATggga                                          >  1:773643/1‑147 (MQ=255)
                                                                                                            ttGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTGGGGTATGAGCGGGTGGGGGTTAATGGGGGTGGCGGGGGCGGTTTTTTTTTTCCGGGGTTTTGAAAGCTGGGTCGCCACTTGGCCGACCTTAGGCGCGGGGGGTAACTgg                                            >  2:604251/1‑146 (MQ=255)
                                                                                                                gCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCtggtg                                   <  1:205226/151‑1 (MQ=255)
                                                                                                                    ttCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGCGGGGGGGTGTTAATTGGGGTGCCGGGGGCTGGTTTTTTTTTCGGGGTTTTCGAAAGCTGGGTCGCCACTGGCCTGACATTAGGCGCGGGGGGTTAATGGAAGCtggtg                                   >  1:409834/1‑147 (MQ=255)
                                                                                                                             gCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGc                      <  1:914194/151‑1 (MQ=255)
                                                                                                                               aTTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGc                      <  1:30991/149‑1 (MQ=255)
                                                                                                                                  aTCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCgtgta                 <  1:196756/151‑2 (MQ=255)
                                                                                                                                    cggcggGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCa               <  2:65554/151‑1 (MQ=255)
                                                                                                                                    cggcggGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCa               <  2:663861/151‑1 (MQ=255)
                                                                                                                                       cggGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATAc            <  1:458807/151‑1 (MQ=255)
                                                                                                                                         ggTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCg          <  2:508953/151‑1 (MQ=255)
                                                                                                                                          gTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGa         <  2:740230/151‑1 (MQ=255)
                                                                                                                                           tGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGa         >  1:541904/1‑150 (MQ=255)
                                                                                                                                                 ggATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATacaac  <  2:690894/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                         
CGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAAC  >  NC_000913/1079321‑1079616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: