Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,413,735 | 0 | A | T | 42.9% | 75.4 / 29.8 | 56 | Y2* (TAT→TAA) | ydaC | DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein |
Reads supporting (aligned to +/- strand): ref base A (5/27); new base T (24/0); total (29/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.79e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATG > NC_000913/1413595‑1413884 | aCTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAac > 2:71311/1‑150 (MQ=255) aCTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAATTaa > 2:264763/1‑149 (MQ=255) ttCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATGAACAcc > 2:494447/1‑150 (MQ=255) gtgatgtgtGGAAAAGTATGGTTCTTTTTTACTTGAGAGCATTTTTTCGCATTGTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATGaaaaaat > 2:87513/9‑144 (MQ=255) cTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATTAAAAACTcc > 2:132987/1‑151 (MQ=255) gCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTcc < 2:402723/149‑1 (MQ=255) ctctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAATGAAAACcccc > 2:646774/1‑146 (MQ=255) ctctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTACATTAAAACCCCCatt > 1:893446/1‑149 (MQ=255) ctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCatta < 2:617885/148‑1 (MQ=255) aaCGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTcc < 1:632772/151‑1 (MQ=255) aaCGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTcc < 1:514668/151‑1 (MQ=255) gCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAAAGAACACCTCCATttttttttt > 2:707111/1‑146 (MQ=255) gCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTTATTAAAACCTCCCTTTTTTTTTTCCGTg > 2:582535/1‑148 (MQ=255) cttcttTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTAAATTAAAACCTCCATTATTATTTTCCGTgg > 1:468905/1‑148 (MQ=255) tcttTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCg < 1:801254/150‑1 (MQ=255) tACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTAAAGAACACCTCCCTTTTTATTTTCAGTGGGTTGtttttt > 1:572427/1‑149 (MQ=255) aCCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTcc < 2:614162/150‑1 (MQ=255) aCCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCa < 1:169785/151‑1 (MQ=255) agCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCg < 1:865812/151‑1 (MQ=255) agCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCg < 1:30223/151‑1 (MQ=255) tttttCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTg < 2:18023/150‑1 (MQ=255) tCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGcttc < 2:687890/150‑1 (MQ=255) cGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAACACCTCCCTTTTTTTTTTTCGTGGGTTGTTTTTTTCATCTTTTTTGTGcttctt > 2:611950/1‑151 (MQ=255) cGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTTcttctt < 2:898667/151‑1 (MQ=255) cGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGcttct < 2:595988/150‑1 (MQ=255) aTTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCAATCTTTCGAGTGCTTCttttt < 1:420823/151‑1 (MQ=255) cTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAACACCTCCCTTATTATTTTCCGTGGTTCGTTTTTTTCCTCTTTTTAGTGCTTCttttt > 1:199831/1‑148 (MQ=255) cTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTATAAAAAAAAACCTCCATTTTTATTTCCAGCGGGTTGTTTTTTCCATTTTTTGCGGGGTTAtttttt > 1:573503/1‑148 (MQ=255) tttCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAACACCCCCCTTTTTTTTTTCAGTGGGTTGTTTTTTTCCTCTTTTGGGTGGttttttttttctttc > 1:904978/1‑144 (MQ=255) aGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAGTAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑a < 1:352403/150‑1 (MQ=255) aGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑aa < 2:458415/151‑1 (MQ=255) gTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AAc < 1:430600/151‑1 (MQ=255) tttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCg < 1:522004/150‑1 (MQ=255) tGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAACACCCCCATTATTATTTTCCGTGGGTCGTTTTTTTCCTTTTTTTTGTGCTTTTTTTTTTCTTTCACC‑AT‑AAACCGGTCGGGGttttcctcc > 1:477413/1‑144 (MQ=255) tCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAACACCCCCCTTTTTATTTTCAGTGGGTTGTTTTTTTCATCTTTCGAGTGCTTTTTTTTTTCTTCCACC‑AT‑AACCGGGTCGGGGTTTGCCTcc > 1:462345/1‑149 (MQ=255) ccAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTATATAAACACCCCCCTTTTTTTTTTCCGTGGGTCGTTTTTTTCATTTTTTTTGGGCTTCTTTTTTTCTTTCCCC‑AT‑AACCCGGTCGGGATTCGCCTCCGtt > 2:78135/1‑151 (MQ=255) cAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTAATGAAAACCTCCCTTTTTATTTTCCGTGGGTTGTTTATTTCATTTTTTCAGGGCTTTTTTTTTTCTTTCACC‑ATAAACCGGTT‑GGGGTTTGCATCCCtt > 1:593778/1‑150 (MQ=255) cAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTGCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTa < 2:209949/151‑1 (MQ=255) tagtagGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAAAACCCCCCCTATTTTTTTCAGGGGGTCGTTTTTTTCATCTTTCTAGTGCTTTTTTTTTTCTTCCCCC‑AT‑AACCCGTTTGGGGTTTTCATCCGtt > 2:39965/1‑146 (MQ=255) gtagGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTc < 1:599592/151‑1 (MQ=255) gtagGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTc < 2:522094/151‑1 (MQ=255) tGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATTAAAACCTCCCTTATTTTTTTCAGTGGTTCGTTTATTTCCTCTTTCGAGGGCTTCTTTTTTACTTCCACC‑AT‑AAACCGTTCGGGATTCGCCTCCGtt > 2:661356/1‑137 (MQ=255) gCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTCATGAACACCCCCATTATTATTTTCAGGGGGTTGTTTTTTTCATCTTTTGCGTGGTTTTTTTTTACTTTCACCAAA‑AACCGGTTGGGGA‑TCGCATTCCTTacaccttccccgg > 1:529236/1‑140 (MQ=255) ccACCTTCCTTTTCAATAGTGGCGGTGATTTTTTTAAATAACACCCCCCTTTTTTTTTTTCGGGGGTCGTTTTTTTTTTCTTTTTGGTGGTTCTTTTTTTCTTTCACC‑CT‑AACCAGGTTGGGGGTCGCATCCCTTTTACTTCCCTc > 2:58269/1‑146 (MQ=255) ccttccttTTCAATAGTGGCGGGAAATTTTTACAATAACACCTCCCTTTTTTTTTTCAGTGGGTTGTTTTTTCCCTCTTTTGGGGGGTTTTTTTTTACTTTCCCC‑AT‑AACCCGGTCGGGGTTCGCATCCGTTACACTTTGCCCCGTaa > 1:757682/1‑148 (MQ=255) cttccttTTCAATAGTGGCGGTAATTTTTTTAAAGAAAACCTCCCTTATTTTTTTTAGTGGGTTGTTTTTTTCATCTTTTTAGTGCTTTTTTTTTTCTTTCACC‑CT‑AAACCGGTCGGGGTTTGCATCCGtt > 2:885205/1‑131 (MQ=255) ttccttTTCAATAGTGGCGGTAATTTTTTACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTTCATCTTTCGAGTGCTTTTTTTTTACTTTCACC‑AT‑AAACGGGTCGGGAATCGCATCCGTTACACTTCGCTCGGTAATATcc > 1:544830/1‑151 (MQ=255) ccttTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCt < 2:843912/150‑1 (MQ=255) cttTTCAATAGTGGCGGTAAATTTTTTTATTAACACCTCCCTTTTTATTTTCTGTGGGTCGTTTTTTTCCTCTTTTCAGTGCTTTTTTTTTTCTTTCCCC‑CT‑AAACGGTTCGGGGTTTGCCTCCGTTTCACTTCCCTCCGTAATATTCt > 2:126260/1‑149 (MQ=255) tttCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGa < 1:286924/149‑1 (MQ=255) tCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAAt < 2:780721/150‑1 (MQ=255) aGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCtt < 1:646857/150‑1 (MQ=255) tGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTc > 1:608447/1‑150 (MQ=255) ggTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGt > 1:829954/1‑151 (MQ=255) gTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGtt < 2:271027/151‑1 (MQ=255) gTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGtt < 2:215772/151‑1 (MQ=255) tataCATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATg > 2:592371/1‑151 (MQ=255) tataCATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATg > 2:877581/1‑151 (MQ=255) | ACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACC‑AT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATG > NC_000913/1413595‑1413884 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |