Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,079,4680AG45.3% 69.1 / 17.2 53D55G (GAT→GGT) putPproline:sodium symporter
Reads supporting (aligned to +/- strand):  ref base A (5/24);  new base G (24/0);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 2.97e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACA  >  NC_000913/1079319‑1079614
                                                                                                                                                     |                                                                                                                                                       
acCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGat                                                                                                                                                        <  1:311518/151‑1 (MQ=255)
        tGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCTGGTATGAGCgg                                                                                                                                                >  2:879522/1‑151 (MQ=255)
                 tttGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑Gt                                                                                                                                     <  2:882735/150‑1 (MQ=255)
                   tgtgTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAgggggcgggtggtt                                                                                                                                      >  2:82128/1‑140 (MQ=255)
                          atatCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTACATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATggg                                                                                                                              <  2:673943/148‑1 (MQ=255)
                           tatCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGGTCGGGTTTGAGGGGGT‑GGG‑T‑GGTTATtggg                                                                                                                             >  2:160970/1‑148 (MQ=255)
                            atCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGGT‑GGC‑T‑GTTTATgggg                                                                                                                             >  2:108000/1‑147 (MQ=255)
                             tCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTAGGAGCGGGC‑GGC‑G‑GTTTATtggggt                                                                                                                           >  1:182945/1‑146 (MQ=255)
                                     aTGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATTAGGGGGT‑GGG‑T‑GGTTATTGGGGTGGCggg                                                                                                                     >  1:212137/1‑146 (MQ=255)
                                      tGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGGATGAGCGGGT‑GGG‑T‑GTTTATTGGGGTGGCgggg                                                                                                                    >  1:882476/1‑145 (MQ=255)
                                          attgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGGTATGAGGGGCG‑GGG‑G‑GGTTATGGGGGGGGCGGGGGGT‑G‑gtttt                                                                                                         >  1:467559/1‑151 (MQ=255)
                                          attgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑tttt                                                                                                          <  1:701219/150‑1 (MQ=255)
                                          attgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGGGGCTGGGG‑G‑GTTATTGGGGGGGCCGGGGCCG‑G‑tttt                                                                                                          >  1:121365/1‑151 (MQ=255)
                                            tgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTtttc                                                                                                        <  2:752352/150‑1 (MQ=255)
                                                   gTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCggg                                                                                                <  2:434978/151‑1 (MQ=255)
                                                    tttATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGGGGGT‑GGCGG‑GTTTTGGGGGTTGCGGGGGCCG‑T‑TTTTTTTTTCCggg                                                                                                >  1:264605/1‑151 (MQ=255)
                                                        tCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGCCTGATATTATGGGGG‑GGG‑T‑GGGAAAGGGGGTGGCGGGCGCG‑G‑Gttttttttttt                                                                                                  >  1:428868/1‑141 (MQ=255)
                                                           ccTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGa                                                                                        <  1:212854/151‑1 (MQ=255)
                                                           ccTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATTGGGGGCT‑GGG‑T‑GTTTATTGGGGGGCCGGGGGCTGG‑TTTTTTTTTTCGGGGtt                                                                                             >  1:207621/1‑147 (MQ=255)
                                                           ccTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGATATGAGCGGCC‑GGG‑C‑GTTTATGGGGGTGGCGGGGGGT‑GTTTTTTTTTTCCGGGTTTTcgt                                                                                         >  2:652916/1‑149 (MQ=255)
                                                            cTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGGTCGGGGATGGGCGGGT‑GGG‑T‑GTTGATTGGGGTGCCGGGGGCT‑G‑Ttttttttttctggggtt                                                                                            >  1:360938/1‑137 (MQ=255)
                                                            cTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTGGGGTATGAGGGGGT‑GGG‑T‑GGTTATTGGGGTGTGGGGGGGT‑G‑Ttttttttttttgggtttt                                                                                           >  2:104810/1‑137 (MQ=255)
                                                            cTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGGGGGGCG‑GGG‑T‑GTTTATTGGGGTGGCGGGGGGG‑G‑Tttttttttttcgggttt                                                                                            >  1:271272/1‑137 (MQ=255)
                                                               gCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGGT‑GGG‑T‑GTTAATGGGGGTGGCGGGGGGT‑G‑TTTTTTTTTTCGGGATTTTCGaaa                                                                                      >  2:689133/1‑149 (MQ=255)
                                                                cGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCt                                                                                   <  1:432718/151‑1 (MQ=255)
                                                                 gATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTg                                                                                  <  1:551750/151‑1 (MQ=255)
                                                                  aTCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTTTGAGCGGGT‑GGG‑T‑GTTAATTGGGGTGGCGGGGGGT‑G‑TTTTTTTTTTCGGGGttttctgaaactgg                                                                                 >  1:905084/1‑140 (MQ=255)
                                                                     aaCGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATc                                                                              <  1:593899/151‑1 (MQ=255)
                                                                       cGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCTGGGATTTCCGAAAGCTGGATCGc                                                                            <  1:488015/151‑1 (MQ=255)
                                                                       cGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGc                                                                            <  2:760185/151‑1 (MQ=255)
                                                                          aaaaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGGCGGATATGAGCGGCC‑GGG‑T‑GTTTATGGGGGTGCCGGGCGCT‑G‑TTTTTTTTTTCCGGGTTTTCGAAAGCTGGATCGccc                                                                          >  2:580712/1‑149 (MQ=255)
                                                                              cTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGCGGCT‑GGG‑G‑GTTTATGGGGGTGGCGGGGGCT‑G‑TTTTTTTTTTCGGGGTTTTCGGAAACTGGGTCGCCAtt                                                                        >  2:761509/1‑148 (MQ=255)
                                                                              cTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATTAGCGGCT‑GGC‑T‑GTTTATGGGGGTGCCGGGGGCT‑G‑TTTTTTTTTTCGGGGTTTCCGAAAGCTGGATCGCCATTgg                                                                      >  2:183551/1‑150 (MQ=255)
                                                                                         atatTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTa                                                            <  2:634584/149‑1 (MQ=255)
                                                                                           atTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGGGGGTATGAGGGGGT‑GGG‑TGGTTATTGGGGTTG‑CGGGGGCC‑G‑TTTTTTTTTTCGGGGTTTTCGGAAGCTGGGTTGCCCTTGGGCTGACATTaaggg                                                        >  2:600101/1‑147 (MQ=255)
                                                                                                   cGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATTAGGGGCT‑GGC‑T‑GTTTTTGGGGGTGGCGGGCGGG‑G‑GTTTTTTTTTCGGGGTTTTCGAAAACTGGGTCGGCATTGGGCCGTCATTTGGCGCggggggt                                                >  1:980620/1‑148 (MQ=255)
                                                                                                    ggTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGGT‑GGC‑T‑GTTTATGGGGGTTCCGGGGGCC‑G‑GTTTTTTTTTCGGGGGTTTCGAAAAGTGGGTCGCCCCTGGGCTGACCATAGGCGCGTGGGtt                                                >  1:577863/1‑150 (MQ=255)
                                                                                                     gTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTaa                                              <  1:628749/151‑1 (MQ=255)
                                                                                                       cGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGGCGGGTATGAGCGGGG‑GGC‑T‑GTTTATGGGGGTTGCGGGGGCT‑G‑TTTTTTTTTTCGGGGTTTTCGAAAGCTGGGTCGCCCTTTGCCTGACATTTGGCGCGGGGGtt                                                >  2:753706/1‑147 (MQ=255)
                                                                                                        gTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTg                                           <  2:897254/151‑1 (MQ=255)
                                                                                                           gTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGaa                                        <  2:83478/151‑1 (MQ=255)
                                                                                                             cTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATTAGCGGCT‑GGC‑T‑GTTTATGGGGGTTCCGGGCGCC‑G‑TTTTTTTTTTCGGGGTTTCCGAAAGCTGGGTCGCCCTTTGCCTGACATTTGGCGCGGGGGTTAACTTGaa                                        >  2:765617/1‑149 (MQ=255)
                                                                                                                gggCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCtgg                                   <  1:84909/151‑1 (MQ=255)
                                                                                                                  gCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCtggtg                                 <  1:468168/151‑1 (MQ=255)
                                                                                                                    cATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATTAGCGGGT‑GGC‑T‑GGTTATTGGGGTGGCGGGGGCC‑G‑TTTTTTTTTTCGGGGGTTTCGAAAAATGGATTGCCATTTGGCTGGCATTATGGGCGGGGGTTTACTGGAAGCtgggggg                               >  1:732806/1‑150 (MQ=255)
                                                                                                                         gTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGc                          <  2:978848/151‑1 (MQ=255)
                                                                                                                              ggCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTg                     <  1:154297/151‑1 (MQ=255)
                                                                                                                                    aTCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCgtgt                <  2:264636/150‑1 (MQ=255)
                                                                                                                                    aTCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGtgtg               <  1:750195/151‑1 (MQ=255)
                                                                                                                                    aTCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGtgtg               <  1:160956/151‑1 (MQ=255)
                                                                                                                                       ggcggGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCAt            <  2:69036/151‑1 (MQ=255)
                                                                                                                                        gcggGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATa           <  1:615806/151‑1 (MQ=255)
                                                                                                                                                gTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATac   >  1:275200/1‑151 (MQ=255)
                                                                                                                                                  cGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATaca  >  1:799626/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
ACCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCT‑GGC‑T‑GTTAATGGGGTTGCCGGGCGCT‑G‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACA  >  NC_000913/1079319‑1079614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑