| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 45.1% | 36.5 / 27.9 | 52 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (18/10); new base A (0/23); total (18/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.73e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAA > NC_000913/357740‑358035 | cTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac > 2:919363/1‑151 (MQ=255) tAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa > 1:951993/1‑150 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaaa > 1:132722/1‑150 (MQ=255) aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGAGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc > 2:884333/1‑150 (MQ=255) gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg < 1:1008604/151‑1 (MQ=255) gcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtct > 2:360138/1‑151 (MQ=255) ccAGCCGCAGCCCTGTGTCAGCGGCTCCCGTGATTCATTTCCGCCAACACCCGCGCTTTTACCCACCGCCATGTGCAAAAATGCTTC‑CGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTg < 2:1006094/149‑1 (MQ=255) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg > 1:758245/1‑151 (MQ=255) ccccgcaCCTTTGACAGCGGTTCCGGTTATTCATTCCCCCAAACACCCCCACTTTTCTCCACCCCCATGTCAAAAAAGGCCTT‑CGCGGCGGCTGTCGGCCATTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg < 1:115071/143‑1 (MQ=255) ccccAGCCCTGTTTCCGCGGCTCCCGTGTTTAATTCCCGCAACCACCCGCGATTTTCTCCACCGCCATGGGAAAAAATCCCTT‑CGGGGGGGTTGTCTGCCAGTTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg < 1:57281/148‑1 (MQ=255) ccGCAGCCCTGTGTCAGCGGCTCCGGTGATTCATTCCCGCCAACACCCGCGCTTTTACCCAACGCCATGTGAAAAAATGCCTC‑CGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCagta < 2:40550/142‑1 (MQ=255) ccGCAGCCCTGTGTCAGCGGCTCCCGTGTTTCATTCCCCCCAACACCCGCGCTTTTACCCAACCCCTTGTGAAAAAATGCCTT‑CGCGGGGGCTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCg < 1:919383/148‑1 (MQ=255) ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:258910/1‑151 (MQ=255) cAGCCCTGTGTCGGGGGCTCCCGTGATTCATTCCCGCAAACACCCCCGCTTTTACCCACCGCCTTGTGAAAAAATGCCTC‑CGGGGGGGCTGTCTCCCGTCTATTTTTCCGCGGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg < 1:950404/146‑1 (MQ=255) aGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggc > 2:8990/1‑147 (MQ=255) tCAGCGGCTCCCTTGATTCTTTCCCCCCAACACCCGCCCTTTTACCCAACCCCATGTGAAAAAATGCCTC‑CGCGGCGGCTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAg < 2:33752/150‑1 (MQ=255) tCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑aaaaa > 2:302734/1‑147 (MQ=255) cGGCTCCCGTGATTCATTTCCCCCAACACCCGCGCTTTTACCCACCGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCGTTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑ttt < 2:503977/150‑1 (MQ=25) ggCTCCCGTGATTCATTCCCGCCAACACCCGCGCATTTACCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTa < 2:237164/150‑1 (MQ=255) ctcccGTGATTCATTCCCCCCAACACCCGCGCTTTTACCCAACGCCATGTGCAAAAATGCCTT‑CGCGGGGGCTGTCTGCCAGCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTaa < 1:823704/146‑1 (MQ=25) cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑AAAAAAC‑ttt > 2:628029/1‑147 (MQ=255) tcccGTGATTCATTCCCGCAAACACCCGCGCATTTACCCACCGCCATGTGCAAAAAGGCCTC‑CGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAg < 1:938377/147‑1 (MQ=255) ccGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑aaaaaaaatttaaac > 1:61807/1‑137 (MQ=25) gTGATTCTTTCCCGCCAACACCCGCGCATTTACCCAACGCCATGTGAAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTTTTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGt < 2:637840/150‑1 (MQ=255) ttCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCATGACG‑AAAAAGC‑TTTaaaacatcatctt > 1:161670/1‑136 (MQ=25) cccGCCAACAACCGCGCATTTATCCAACGCCATGTGAAAAAATGCCTC‑CGCGGCGGCTGTCTGCCAGCTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTtgtgtg < 2:53662/150‑1 (MQ=255) cccccacaacccGCGCATTAACCCAACGCATGGAGAAAAAATGCTTC‑CGCGGCGGCTGTCGGCCAGTTTTTTTCCCGCCAAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTtgtg < 1:461832/138‑1 (MQ=255) caacaaCCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑G‑AAAAAAA‑TTTAAACCCGGCATGTTGTGTGGGaa > 2:360363/1‑150 (MQ=37) acaacCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGG‑G‑AAAAAAA‑TTTAAAACCCCCCTGTTGTGTgggga > 2:92488/1‑145 (MQ=25) acCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCATAA‑GAAAAAAGC‑TTTAAACCC‑CCTTGTTGTGTGGcgaacc > 2:47901/1‑143 (MQ=25) ccGCGCTTTTACCCACCGCCTTGTGAAAAAATCCTTC‑CGCGGCGGCTTTCCGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATc < 1:779138/146‑1 (MQ=255) ccGCGCTTTTACCCACCCCCTTGTGAAAAAATGCCTT‑CGGGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCgctgc < 1:280207/151‑1 (MQ=255) gcttttACCCAACCCCATGTCAAAAAATCCTTT‑CGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGt < 2:164231/148‑1 (MQ=255) aTCCACCGCCATGTGCAAAATTCCCTT‑CGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACa < 2:405279/149‑1 (MQ=255) caccGCCATGTG‑AAAAATTCCTTCCGCGGCGGCTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAt‑gtg < 2:698493/148‑1 (MQ=255) aaCGCCATGTAAAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTgg < 2:892476/151‑1 (MQ=255) tgaaaaaaTGCCTT‑CGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGa < 2:776130/146‑1 (MQ=255) aaaaaTGCCTT‑CGCGGCGGCTGTCTCCCAGCTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGa < 1:755450/146‑1 (MQ=255) aaaTGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGAAAGA‑G‑AAAAAGA‑TTTTAACCCCCCCTGTTTTGTGGGGAACGCTGCATGTAACAATGGGGGAAAGGGagggggg > 2:910811/1‑145 (MQ=255) gCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑G‑AAAAAAC‑TTTTAACCCCCCCTGTTGTGTGGGGATAGCTGCTGGGAACAAA‑GTGGAAAGGGGAgggggggg > 2:873545/1‑142 (MQ=255) cttt‑CGGGGCGGTTGTTTGCTAGATTTTTTCCCCCCGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGaa < 2:629261/148‑1 (MQ=255) cttccgcgGCGGCTGTCTGCCGGCTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGa < 2:374370/145‑1 (MQ=255) gcggcgGCTTTCTGCCAGTTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCa < 2:80058/148‑1 (MQ=255) gcggcgGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGCTCTCTCCAGTAGTGGCGGGGGCAAA‑G‑AAAAAACATTTAAACCC‑CCATGTTGTGTGGGGAACTCTGGTGGTAAAAAA‑GTGGGAAGGGGAgggggg > 2:436789/1‑136 (MQ=255) ggcggcTTTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCt < 2:296178/149‑1 (MQ=255) ggcggcTTCCTGCCGGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCTc < 1:629295/150‑1 (MQ=255) tcggCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAcc < 2:823446/146‑1 (MQ=255) ccAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgcg < 2:686178/148‑1 (MQ=255) ccAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgc < 2:1020616/147‑1 (MQ=255) ccAGCTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgc < 2:647921/147‑1 (MQ=255) tttcCCGCCAAAAGAAAACCGCCGTTCTCGCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGt < 1:884232/150‑1 (MQ=255) gccgcAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑G‑GAATAACATTAAAACCCTCCTTGTTG‑GTGGGAAAGGGTGGTGGGAAAAAA‑GTGGGGAGGGGAATGGGGGGGATGAGCTCCCAAACACCGCTAAATGAggtag > 2:144759/1‑143 (MQ=255) ccgcAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCa < 2:663544/151‑1 (MQ=255) caacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑AAAAAAA‑ATTAAAACCCCCCCGTTGTTTGGGGATAGCTGCTGGGGAAAAA‑ATGGGAAGGGAGGGGCGGGGGAACAGCTCCAGAACACCGCTAATTTAGTTTGCCCCaa > 1:484517/1‑149 (MQ=255) caacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑G‑AAAAAAA‑TTTTAACCCCCCCCTTTGGGTGGGGAGCGCTTCTGGGAAAAAA‑GTGGGAAAGGGAGTGCGGCGGATCAGACCCCCAAACACGCCAAATGAGTTGGCCTcca > 1:920282/1‑147 (MQ=255) | CTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑CGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAT‑GTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAA > NC_000913/357740‑358035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |