Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913941,7130TG52.2% 51.4 / 73.8 46V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (2/20);  new base G (24/0);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 4.12e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-08

TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941568‑941862
                                                                                                                                                 |                                                                                                                                                     
tactCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCtt                                                                                                                                                  >  2:993787/1‑151 (MQ=255)
 actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACtt                                                                                                                                                  <  1:891269/150‑1 (MQ=255)
 actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACtt                                                                                                                                                  <  1:235997/150‑1 (MQ=255)
 actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTa                                                                                                                                                 <  1:920892/151‑1 (MQ=255)
   tcctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACtt                                                                                                                                                  >  2:293566/1‑148 (MQ=255)
       ccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATc                                                                                                                                            <  2:645748/150‑1 (MQ=255)
          cgcAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGa                                                                                                                                        <  1:503545/151‑1 (MQ=255)
            cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCtttta                                                                                                                                       >  2:905837/1‑147 (MQ=255)
            cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGaa                                                                                                                                       >  2:873876/1‑150 (MQ=255)
               aTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAgg                                                                                                                                   >  2:413487/1‑151 (MQ=255)
                   cGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTAATATTTTTAACAgg                                                                                                                                   >  1:696363/1‑147 (MQ=255)
                    ggAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCTTGAACAGGCAc                                                                                                                                >  1:618089/1‑149 (MQ=255)
                      aaGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCa                                                                                                                            <  2:287513/151‑1 (MQ=255)
                              aaCTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACGGGCACGCCAGAAATCt                                                                                                                     >  2:645215/1‑150 (MQ=255)
                                      cTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGACTACTATCTTTTAAAGGCACGCCCGAAAAATAAAGcc                                                                                                               >  1:311742/1‑148 (MQ=255)
                                          ggcggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGc                                                                                                            >  2:769423/1‑147 (MQ=255)
                                           gcggcTGGGCAGATGGCAACAGCCTGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAATTACTATCTTGAACAGGCACGCCAGAAAACTAATGCCCGCATGat                                                                                                       >  1:578929/1‑151 (MQ=255)
                                            cggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGa                                                                                                        >  2:624200/1‑149 (MQ=255)
                                                   gCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCg                                                                                               <  2:614297/151‑1 (MQ=255)
                                                         ggCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCcg                                                                                          <  2:233048/150‑1 (MQ=255)
                                                           cAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAATCTAAAGCCCGCATGATCATCATCGATcc                                                                                           >  1:171753/1‑147 (MQ=255)
                                                           cAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTATGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCgcgc                                                                                       <  2:167032/151‑1 (MQ=255)
                                                            aaCAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTATTTTTTTTAAAAGGCACCCCCGAAATTTAATGCCCCCCTGATCATAATCGATcc                                                                                           >  2:796125/1‑146 (MQ=255)
                                                              cAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCtat                                                                                    <  1:132214/151‑1 (MQ=255)
                                                               aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCtat                                                                                    >  1:678428/1‑150 (MQ=255)
                                                                   cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAGTTAATATTTTTAAAAGGGAAGCCAGAAATATAATGGCCGCATGATTATTATTTATACGCGCta                                                                                     >  1:300579/1‑145 (MQ=255)
                                                                        gATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCGACAcc                                                                           >  1:302005/1‑150 (MQ=255)
                                                                                 aaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgc                                                                 <  1:477787/151‑1 (MQ=255)
                                                                                   cAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcgc                                                               <  1:999804/151‑1 (MQ=255)
                                                                                      tAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAGCTATTATCTTGAAAACGGACGCCAGAAATCTAATGCCCGCATGATCATCATCGCTCCCCGCTATACCGGCACccgggccggg                                                                  >  2:552571/1‑145 (MQ=255)
                                                                                        aGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcg                                                                >  1:558458/1‑145 (MQ=255)
                                                                                            ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGa                                                       <  1:614484/150‑1 (MQ=255)
                                                                                                 tGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATATCTTGAAAAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCATCGGTCCGCGCTATACCGCCACCGGGGCCGGGGGCGAAGATGAGTgg                                                   >  1:863314/1‑149 (MQ=255)
                                                                                                         ggTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTAtt                                          <  1:595284/150‑1 (MQ=255)
                                                                                                         ggTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTc                                         <  2:914024/151‑1 (MQ=255)
                                                                                                           taataaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGGAGATGAGTGGATCCCTAtt                                          >  2:842830/1‑148 (MQ=255)
                                                                                                            aataaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGGGGATCCCTATTCGt                                       >  1:281070/1‑150 (MQ=255)
                                                                                                                        gAAACGCGAATGAGTGGCGGCGGGGGGACTTACTATCTTGAACAAGCACGCCAGAAATCTAATGGCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGAGCCGGGGGCGAAGATGAGTGGATTCCCATTCGTCCGGGAACAGa                            >  2:317278/1‑149 (MQ=255)
                                                                                                                          aaCGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAAAAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGGAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGcc                        >  1:998056/1‑151 (MQ=255)
                                                                                                                           aCGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAAGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCCGGGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGGACAGATGcc                        >  1:174744/1‑150 (MQ=255)
                                                                                                                                aaTGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTg                  <  1:993665/151‑1 (MQ=255)
                                                                                                                                       ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACg           >  2:632780/1‑151 (MQ=255)
                                                                                                                                        gCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACgg          <  1:758957/151‑1 (MQ=255)
                                                                                                                                              gggTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGc    <  2:41551/151‑1 (MQ=255)
                                                                                                                                               ggTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCg   <  1:937694/151‑1 (MQ=255)
                                                                                                                                                gTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt  <  2:173439/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941568‑941862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑