Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,356,672	A→G	Y147H (TAC→CAC)	sapA ←	antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,356,672	0	A	G	100.0%	333.9 / NA	84	Y147H (TAC→CAC)	sapA	antimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/39);  total (45/39)

TCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAA  >  NC_000913/1356530‑1356819                                                                                                                                               |                                                                                                                                                    tCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGt                                                                                                                                             >  1:715026/1‑151 (MQ=255)  cTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGGGGGGAAGtt                                                                                                                                            >  1:149773/1‑151 (MQ=255)   tGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTtg                                                                                                                                           >  1:982689/1‑151 (MQ=255)    gCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTtg                                                                                                                                           <  1:615604/150‑1 (MQ=255)             aCCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTg                                                                                                                                  >  1:502705/1‑150 (MQ=255)                gAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGt                                                                                                                              <  1:567848/151‑1 (MQ=255)                  aGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGtt                                                                                                                             >  1:32866/1‑150 (MQ=255)                    cATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATg                                                                                                                          >  2:236709/1‑151 (MQ=255)                     aTAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGc                                                                                                                         >  2:176531/1‑151 (MQ=255)                      tAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGcc                                                                                                                        >  2:518309/1‑151 (MQ=255)                       aaTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGcc                                                                                                                        >  2:337978/1‑150 (MQ=255)                          gggTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACg                                                                                                                     <  2:196484/150‑1 (MQ=255)                             ttGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGgtt                                                                                                                 >  2:16657/1‑151 (MQ=255)                             ttGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGAATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGgtt                                                                                                                 >  2:593387/1‑151 (MQ=255)                              tGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGgtt                                                                                                                 <  2:336419/150‑1 (MQ=255)                                  aGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGc                                                                                                            <  1:1001534/151‑1 (MQ=255)                                  aGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGc                                                                                                            <  2:463043/151‑1 (MQ=255)                                  aGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGc                                                                                                            >  2:854901/1‑151 (MQ=255)                                      gCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTc                                                                                                        >  2:666066/1‑151 (MQ=255)                                       ccACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCa                                                                                                       <  1:421055/151‑1 (MQ=255)                                        cacaAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCa                                                                                                       >  2:654970/1‑150 (MQ=255)                                        cacaAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCa                                                                                                       >  2:302860/1‑150 (MQ=255)                                         acaAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCaaa                                                                                                     <  2:361762/151‑1 (MQ=255)                                         acaAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCaa                                                                                                      >  1:384749/1‑150 (MQ=255)                                           aaaaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCaaaa                                                                                                    >  1:728539/1‑150 (MQ=255)                                            aaaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAAt                                                                                                  >  2:310738/1‑151 (MQ=255)                                                  aagCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTg                                                                                            >  2:87881/1‑151 (MQ=255)                                                    gCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGa                                                                                          <  1:603351/151‑1 (MQ=255)                                                     cATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGaa                                                                                         <  1:846335/151‑1 (MQ=255)                                                      aTCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGa                                                                                          >  2:502595/1‑149 (MQ=255)                                                      aTCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGa                                                                                          >  2:439729/1‑149 (MQ=255)                                                      aTCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAg                                                                                        <  1:122575/151‑1 (MQ=255)                                                       tCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAgg                                                                                       >  2:240685/1‑151 (MQ=255)                                                       tCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAgg                                                                                       >  1:512680/1‑151 (MQ=255)                                                          ggCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaa                                                                                    <  1:568612/151‑1 (MQ=255)                                                          ggCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaa                                                                                    <  1:828455/151‑1 (MQ=255)                                                          ggCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGATGTTGCGGTCAAAAATTCGCTGGAAGGt                                                                                      >  1:89573/1‑149 (MQ=255)                                                             tGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAac                                                                                  >  1:314861/1‑150 (MQ=255)                                                              gggCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAacac                                                                                <  1:737481/151‑1 (MQ=255)                                                                  gAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACcaca                                                                            <  1:632647/151‑1 (MQ=255)                                                                    gTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtc                                                                          >  1:897179/1‑151 (MQ=255)                                                                       ggAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtcgtc                                                                       >  2:409403/1‑151 (MQ=255)                                                                        gAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtcgtcg                                                                      >  2:712669/1‑151 (MQ=255)                                                                         aaCTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCgg                                                                     >  1:321527/1‑151 (MQ=255)                                                                          aCTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGc                                                                    <  2:321568/151‑1 (MQ=255)                                                                             cAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcatt                                                                 <  1:575740/151‑1 (MQ=255)                                                                             cAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcat                                                                  <  1:998796/150‑1 (MQ=255)                                                                             cAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGca                                                                   <  2:21794/149‑1 (MQ=255)                                                                                cGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcattcat                                                              >  1:726720/1‑151 (MQ=255)                                                                                 ggTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcattcatt                                                             >  2:351196/1‑151 (MQ=255)                                                                                      gATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTAc                                                         <  1:594694/150‑1 (MQ=255)                                                                                       aTTATCCAGTTTGCGGACGCTTTTCACTTTATCGGCAAATTGCAGGCTGTCGAAGGGGGGGAAGTTGCTGCCGTTGTCGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGa                                                       <  1:43923/151‑1 (MQ=255)                                                                                        ttATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACg                                                        >  2:359977/1‑149 (MQ=255)                                                                                         tATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGt                                                     >  2:94839/1‑151 (MQ=255)                                                                                          aTCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTg                                                    <  2:217055/151‑1 (MQ=255)                                                                                           tCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTgg                                                   >  2:353745/1‑151 (MQ=255)                                                                                           tCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTgg                                                   >  1:848997/1‑151 (MQ=255)                                                                                             cAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTggg                                                  <  2:308317/150‑1 (MQ=255)                                                                                              aGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTggg                                                  >  1:450048/1‑149 (MQ=255)                                                                                              aGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAg                                                <  2:412300/151‑1 (MQ=255)                                                                                                  tGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTaa                                             <  1:487907/150‑1 (MQ=255)                                                                                                   gCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAAc                                           <  2:897282/151‑1 (MQ=255)                                                                                                    cGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAAcc                                          >  1:1018237/1‑151 (MQ=255)                                                                                                               cACGTTATCGGCAAATTGCAGGCTTTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGtttt                                <  1:547824/150‑1 (MQ=255)                                                                                                               cACGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGttttt                               <  1:123931/151‑1 (MQ=255)                                                                                                                aCGTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGtttttt                              <  1:593721/151‑1 (MQ=255)                                                                                                                  gTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTg                             >  2:329016/1‑150 (MQ=255)                                                                                                                  gTTATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGa                            <  1:804022/151‑1 (MQ=255)                                                                                                                    tATCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGaa                           >  1:987261/1‑150 (MQ=255)                                                                                                                     aTCGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAAc                         <  1:302823/151‑1 (MQ=255)                                                                                                                       cGGCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACg                        <  2:924892/150‑1 (MQ=255)                                                                                                                         gCAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGAGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGaa                     <  2:324404/151‑1 (MQ=255)                                                                                                                          cAAATTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAAc                    <  1:600600/151‑1 (MQ=255)                                                                                                                           aaaTTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCGGTCGGTTTTTTGAAACGGAACa                   <  1:465365/151‑1 (MQ=255)                                                                                                                            aaTTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAt                  <  1:79268/151‑1 (MQ=255)                                                                                                                            aaTTGCAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACAt                  <  1:355480/151‑1 (MQ=255)                                                                                                                                 cAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATcgcg              >  1:956672/1‑150 (MQ=255)                                                                                                                                 cAGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATcgcg              >  2:772055/1‑150 (MQ=255)                                                                                                                                  aGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGa             >  1:503236/1‑150 (MQ=255)                                                                                                                                  aGGCTGTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGAc            >  2:670491/1‑151 (MQ=255)                                                                                                                                       gTCGAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAg        <  2:770570/150‑1 (MQ=255)                                                                                                                                          gAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTgg    >  1:708983/1‑151 (MQ=255)                                                                                                                                          gAAGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTgg    >  1:36749/1‑151 (MQ=255)                                                                                                                                            aGTGGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGaa  <  2:16016/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    TCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAA  >  NC_000913/1356530‑1356819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑