Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT63.6% ‑6.3 / 51.2 55intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (4/16);  new base T (35/0);  total (39/16)
Fisher's exact test for biased strand distribution p-value = 1.63e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-05

CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGC  >  NC_000913/3206285‑3206580
                                                                                                                                                      |                                                                                                                                                  
cAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTc                                                                                                                                                    <  1:961854/151‑1 (MQ=255)
 aGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTcc                                                                                                                                                   <  2:751402/151‑1 (MQ=255)
     tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGTAATTTTcaa                                                                                                                                                  >  1:456452/1‑146 (MQ=255)
        ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACaa                                                                                                                                             >  2:179075/1‑150 (MQ=255)
           gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACcaaaa                                                                                                                                          >  1:661093/1‑148 (MQ=255)
           gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                         <  2:84371/151‑1 (MQ=255)
             aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACaaa                                                                                                                                            >  1:249481/1‑146 (MQ=255)
                     gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACTg                                                                                                                                >  2:378244/1‑149 (MQ=255)
                     gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                              <  2:248015/151‑1 (MQ=255)
                       cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAACTGGAg                                                                                                                             >  1:661985/1‑150 (MQ=255)
                              tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCat                                                                                                                      >  1:523622/1‑150 (MQ=255)
                              tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAAAAAAACTGGAGTTGcc                                                                                                                        >  1:9256/1‑148 (MQ=255)
                                  ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGTTGGCatgat                                                                                                                   >  2:428535/1‑149 (MQ=255)
                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAATAAAAAAAATGGAGTTGGCATGATGAGCg                                                                                                              >  1:497806/1‑147 (MQ=255)
                                         aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATACAAAAAATGGAGGTGGCATGATGAGCGgaa                                                                                                           >  1:141657/1‑150 (MQ=255)
                                             ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAAACAAAAATGGAGGTGCCCTGATGGGCGAAAGtaa                                                                                                       >  2:8207/1‑150 (MQ=255)
                                                attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATACAAAAAATGGAGGTTCCATGATGAGCGAAAAaaataa                                                                                                    >  1:47447/1‑150 (MQ=255)
                                                attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAg                                                                                                   <  1:501024/151‑1 (MQ=255)
                                                      aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                              <  2:534615/150‑1 (MQ=255)
                                                        tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGTTGTCATGATGAGCGAAAGTAATAAACaaaa                                                                                              >  2:783257/1‑146 (MQ=255)
                                                          tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGGGTTGCCATGATGAGCGGAAATAATAAACaaaaag                                                                                            >  2:3139/1‑144 (MQ=255)
                                                             cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAACTGGAGTTGGCATGATGAGCGAAAAGAATAAACAAAAAGCAGTg                                                                                       >  1:898663/1‑150 (MQ=255)
                                                                        gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGGAAGAAATAAGCAAAAAGCAGGGAATAAGtt                                                                               >  1:159832/1‑147 (MQ=255)
                                                                        gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAAGCAGTGAATAAGTTGAc                                                                            >  2:304877/1‑150 (MQ=255)
                                                                            tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAAATGGAGTTGGCATGATGAGCGGAAGAAATAAACAAAAAGCAGTGAATAAGGTGACa                                                                           >  1:973623/1‑147 (MQ=255)
                                                                             gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag                                                                        <  1:75588/149‑1 (MQ=255)
                                                                             gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag                                                                        <  1:930924/149‑1 (MQ=255)
                                                                              ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGGGTTGTCCTGATGAGCGAAAAAAATAAGCAACAGGCAGGGAATAAGTTGACAGAGAt                                                                      >  2:8327/1‑150 (MQ=255)
                                                                                   tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATACAAAAACTGGAGTTTGCATGATGAGCGAAAGTAATAAACAAAAAGCAGGGAAAAAATTGGAAGAGATTGTCg                                                                 >  1:282101/1‑150 (MQ=255)
                                                                                    aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATACAAAAACTGGAGTTGGCATGATGAGGGAAAGTAATAAAAAAAAAGCAGAGAATAAATTGACAGAGATTGTGGCt                                                               >  1:726526/1‑151 (MQ=255)
                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAATACAAAAAATGGAGTTGGCATGATGAGGGAAAAGAATAAAGAACAGGGAGGGAATAAGTTGACAAAGATTGTTGCt                                                               >  1:720842/1‑149 (MQ=255)
                                                                                       tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGGGAAAGTAATAAACAAAAGGCAGGGAATAAGTTGACAGAGATTGTCGCTaa                                                             >  2:834457/1‑150 (MQ=255)
                                                                                        atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAAATGGAGTTGGCATGGTGGGCGGAAATAATAAGCAAAAGGCAGTGAATAAAATTAAAGAGATTTTCGCTAAc                                                            >  1:371845/1‑150 (MQ=255)
                                                                                          tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                          <  1:549283/150‑1 (MQ=255)
                                                                                          tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAACAAAAAATGGAGTTGGCATGATGAGCGAAAGTAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTTTCGCTAAAttt                                                         >  1:226939/1‑151 (MQ=255)
                                                                                           cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                        <  2:81306/151‑1 (MQ=255)
                                                                                           cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGTATTTTTCCCAACAAATACAAAAAATGGAGGTTCCATGATGCAGGAAAGGAAAAAGCAAAAGGGAATGAATAAATTGACAGAGATTTTCGCTaaactt                                                         >  1:988533/1‑146 (MQ=255)
                                                                                             cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGTTGGCATGATGAGCGGAAGGAAAAAACAAAAGGCAGGGAATAAATTGAAAGAGATTGTCGCCAACTTTTcc                                                      >  1:24119/1‑151 (MQ=255)
                                                                                                 ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAAACAAAAACAAAAAAAGGGGGTTGCCTGATGAGCGAAAGGAAAAAACAAAAAGCAGGGAATAAAATTACAGAGAATGTTGCTAAATTTaacccc                                                   >  1:516878/1‑145 (MQ=255)
                                                                                                       aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGAATATCGCTAAATTTACCGCCATGAttt                                            >  1:735509/1‑151 (MQ=255)
                                                                                                        aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAGCAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                                            >  2:692938/1‑150 (MQ=255)
                                                                                                        aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCCTGATGAGCGAAAGTAATAAGCAACAAGCAGGGAATAAAGTGGAAGAGATTGTCGCTAACTTTACCGCCATGAttt                                            >  2:380316/1‑150 (MQ=255)
                                                                                                                   acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGGCCTGATGAGCGAAAGAAAAAAACAACAGGCAGGGAATAAGTTTACAAAGAATGTCGCTAACTTTAACGCCATGATTTTTACCCGaa                                   >  2:363384/1‑148 (MQ=255)
                                                                                                                       ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAAGCAGTGAATAAGTTGACAGAGATTGTCGCTCACTTTTCCCCCATGATTTTTTCCCCAATGcc                               >  2:305103/1‑148 (MQ=255)
                                                                                                                         ttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                           <  1:830339/150‑1 (MQ=255)
                                                                                                                              ggTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                     <  2:160303/151‑1 (MQ=255)
                                                                                                                              ggTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                        <  1:207948/148‑1 (MQ=255)
                                                                                                                              ggTAGTTTGTGGCGGTGAAATTTTTCAGACAAATACAAAAAAAGGAGTTGGCATGATGAGCGAAAGTAATAAGCAACAGGCAGGGAATAAGTTGATAGAGATTTTAGCCAACCTTACCGCCAAGATTTCTACCCGAATGCCTGATGACggg                     >  1:123170/1‑149 (MQ=255)
                                                                                                                                 aGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGGCATGATGAGCGAAAGTAATAAGCAACAAGCAGTGAATAAGTTGACAGAGATTGTCGCTAAATTTACCGCCATGATTTCTACCCGAATACCTGATGACgtgggg                  >  2:885780/1‑149 (MQ=255)
                                                                                                                                   tttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                 <  2:249499/150‑1 (MQ=255)
                                                                                                                                      gtgGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCGAATGCCTGATGACGTGGGGGGTa              >  1:966023/1‑150 (MQ=255)
                                                                                                                                       tgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa             <  2:523666/150‑1 (MQ=255)
                                                                                                                                         gCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAAATTGTCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt          <  1:402938/151‑1 (MQ=255)
                                                                                                                                           ggTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa         >  1:282849/1‑150 (MQ=255)
                                                                                                                                             tGAATTTTTCCAGACAAATACAAAAACTTGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGACAGCCTGATGACGTGGAGGATAAACTaaa       >  1:404770/1‑150 (MQ=255)
                                                                                                                                             tGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa       >  1:811242/1‑150 (MQ=255)
                                                                                                                                               aaTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa    <  2:180954/151‑1 (MQ=255)
                                                                                                                                                  ttttCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGAAAAAAGCAACAGGCATTGATTAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc  <  1:258843/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGC  >  NC_000913/3206285‑3206580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: