Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,452 | 0 | C | A | 44.8% | 56.6 / 32.2 | 58 | intergenic (‑196/‑11) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (13/19); new base A (26/0); total (39/19) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.20e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTC > NC_000913/3206306‑3206599 | gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAACTg > 2:378244/1‑149 (MQ=255) gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGa < 2:248015/151‑1 (MQ=255) cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAACTGGAg > 1:661985/1‑150 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATGGAGGTGGCat > 1:523622/1‑150 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAA‑AAAAACTGGAGTTGcc > 1:9256/1‑148 (MQ=255) ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAA‑AAAAAATGGAGTTGGCatgat > 2:428535/1‑149 (MQ=255) aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAATAA‑AAAAAATGGAGTTGGCATGATGAGCg > 1:497806/1‑147 (MQ=255) aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAC‑AAAAAATGGAGGTGGCATGATGAGCGgaa > 1:141657/1‑150 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAAA‑CAAAAATGGAGGTGCCCTGATGGGCGAAAGtaa > 2:8207/1‑150 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAC‑AAAAAATGGAGGTTCCATGATGAGCGAAAAaaataa > 1:47447/1‑150 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAg < 1:501024/151‑1 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa < 2:534615/150‑1 (MQ=255) tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAA‑AAAAAATGGAGTTGTCATGATGAGCGAAAGTAATAAACaaaa > 2:783257/1‑146 (MQ=255) tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAAATGGGGTTGCCATGATGAGCGGAAATAATAAACaaaaag > 2:3139/1‑144 (MQ=255) cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAA‑AAAAACTGGAGTTGGCATGATGAGCGAAAAGAATAAACAAAAAGCAGTg > 1:898663/1‑150 (MQ=255) gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGTTGCCATGATGAGCGGAAGAAATAAGCAAAAAGCAGGGAATAAGtt > 1:159832/1‑147 (MQ=255) gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAAGCAGTGAATAAGTTGAc > 2:304877/1‑150 (MQ=255) tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAA‑AAAAAATGGAGTTGGCATGATGAGCGGAAGAAATAAACAAAAAGCAGTGAATAAGGTGACa > 1:973623/1‑147 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag < 1:930924/149‑1 (MQ=255) gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag < 1:75588/149‑1 (MQ=255) ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGGGTTGTCCTGATGAGCGAAAAAAATAAGCAACAGGCAGGGAATAAGTTGACAGAGAt > 2:8327/1‑150 (MQ=255) tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAC‑AAAAACTGGAGTTTGCATGATGAGCGAAAGTAATAAACAAAAAGCAGGGAAAAAATTGGAAGAGATTGTCg > 1:282101/1‑150 (MQ=255) aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAC‑AAAAACTGGAGTTGGCATGATGAGGGAAAGTAATAAAAAAAAAGCAGAGAATAAATTGACAGAGATTGTGGCt > 1:726526/1‑151 (MQ=255) atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAATAC‑AAAAAATGGAGTTGGCATGATGAGGGAAAAGAATAAAGAACAGGGAGGGAATAAGTTGACAAAGATTGTTGCt > 1:720842/1‑149 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGTTGCCATGATGAGGGAAAGTAATAAACAAAAGGCAGGGAATAAGTTGACAGAGATTGTCGCTaa > 2:834457/1‑150 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAA‑AAAAAATGGAGTTGGCATGGTGGGCGGAAATAATAAGCAAAAGGCAGTGAATAAAATTAAAGAGATTTTCGCTAAc > 1:371845/1‑150 (MQ=255) tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt < 1:549283/150‑1 (MQ=255) tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAC‑AAAAAATGGAGTTGGCATGATGAGCGAAAGTAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTTTCGCTAAAttt > 1:226939/1‑151 (MQ=255) cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa < 2:81306/151‑1 (MQ=255) cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGTATTTTTCCCAACAAATAC‑AAAAAATGGAGGTTCCATGATGCAGGAAAGGAAAAAGCAAAAGGGAATGAATAAATTGACAGAGATTTTCGCTaaactt > 1:988533/1‑146 (MQ=255) cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAC‑AAAAAATGGAGTTGGCATGATGAGCGGAAGGAAAAAACAAAAGGCAGGGAATAAATTGAAAGAGATTGTCGCCAACTTTTcc > 1:24119/1‑151 (MQ=255) ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAAACAAAAACAAAAAAAGGGGGTTGCC‑TGATGAGCGAAAGGAAAAAACAAAAAGCAGGGAATAAAATTACAGAGAATGTTGCTAAATTTaacccc > 1:516878/1‑145 (MQ=255) aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGAATATCGCTAAATTTACCGCCATGAttt > 1:735509/1‑151 (MQ=255) aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAGCAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt > 2:692938/1‑150 (MQ=255) aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATGGAGTTGCCCTGATGAGCGAAAGTAATAAGCAACAAGCAGGGAATAAAGTGGAAGAGATTGTCGCTAACTTTACCGCCATGAttt > 2:380316/1‑150 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGTTGGCCTGATGAGCGAAAGAAAAAAACAACAGGCAGGGAATAAGTTTACAAAGAATGTCGCTAACTTTAACGCCATGATTTTTACCCGaa > 2:363384/1‑148 (MQ=255) ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAAGCAGTGAATAAGTTGACAGAGATTGTCGCTCACTTTTCCCCCATGATTTTTTCCCCAATGcc > 2:305103/1‑148 (MQ=255) ttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat < 1:830339/150‑1 (MQ=255) ggTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 2:160303/151‑1 (MQ=255) ggTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc < 1:207948/148‑1 (MQ=255) ggTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAC‑AAAAAAAGGAGTTGGCATGATGAGCGAAAGTAATAAGCAACAGGCAGGGAATAAGTTGATAGAGATTTTAGCCAACCTTACCGCCAAGATTTCTACCCGAATGCCTGATGACggg > 1:123170/1‑149 (MQ=255) aGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGGCATGATGAGCGAAAGTAATAAGCAACAAGCAGTGAATAAGTTGACAGAGATTGTCGCTAAATTTACCGCCATGATTTCTACCCGAATACCTGATGACgtgggg > 2:885780/1‑149 (MQ=255) tttGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg < 2:249499/150‑1 (MQ=255) gtgGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCGAATGCCTGATGACGTGGGGGGTa > 1:966023/1‑150 (MQ=255) tgGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa < 2:523666/150‑1 (MQ=255) gCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAAATTGTCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt < 1:402938/151‑1 (MQ=255) ggTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa > 1:282849/1‑150 (MQ=255) tGAATTTTTCCAGACAAATAC‑AAAAACTTGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGACAGCCTGATGACGTGGAGGATAAACTaaa > 1:404770/1‑150 (MQ=255) tGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa > 1:811242/1‑150 (MQ=255) aaTTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa < 2:180954/151‑1 (MQ=255) ttttCCAGAAAAATAA‑AAAAACTGGAGTTGCCATGATGAGCAAAAGAAAAAAGCAACAGGCATTGATTAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc < 1:258843/150‑1 (MQ=255) gACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAt > 2:484815/1‑151 (MQ=255) aaaaaTAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATg < 2:715778/149‑1 (MQ=255) aaaaaTAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAt < 1:866219/148‑1 (MQ=255) aaaTAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGAAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGc < 1:840986/150‑1 (MQ=255) aTAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCg > 2:151710/1‑150 (MQ=255) aaaaaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcg > 1:562007/1‑151 (MQ=255) aaaaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgt < 2:662049/151‑1 (MQ=255) aaccTGGAGTTGCCATGATGAGCGAAAGTAAAAAGAAACAGGCAGTAAATAAGTTGAAAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtc < 1:1023010/148‑1 (MQ=255) | GCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTC > NC_000913/3206306‑3206599 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |