Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091373,5810AC44.6% 115.0 / 11.4 65V314G (GTA→GGA) thiPthiamine/thiamine pyrophosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (30/6);  new base C (0/29);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 6.53e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGG  >  NC_000913/73437‑73722
                                                                                                                                                |                                                                                                                                             
cgcgCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCAt                                                                                                                                         >  1:609267/1‑151 (MQ=255)
    cccGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcgac                                                                                                                                     <  2:437389/151‑1 (MQ=255)
         aGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATc                                                                                                                                 >  1:1609/1‑150 (MQ=255)
            tCGCGACTGCTCCATATCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCg                                                                                                                             >  2:397778/1‑151 (MQ=255)
                 aCTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGt                                                                                                                        >  1:948318/1‑151 (MQ=255)
                  cTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGt                                                                                                                        >  2:627725/1‑150 (MQ=255)
                   tGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTa                                                                                                                       >  1:506540/1‑150 (MQ=255)
                       ccATAGCAGCATCAGGGCCAGCACTCCCCAAAATCCCCCGGCCGCCAGCGAAATACGCACCGGGGCCCACGGCGCCTCCCACAGCCCCGGTTGTGCCAGCCCTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACg                                                                                                                    <  1:434865/149‑1 (MQ=255)
                       ccATAACAGCATCATGGTCACCCCCCCGCAAAATCCCCCTGCCGCCGGCGCAATCCGCAACGGGGCCCACAGCGCCTGCCACAGCCCGGGTTGCGCCAGCACTTCCGGCAACTGGCTATTTCCCCCATCGACGATCACCGCCAGTAACg                                                                                                                    <  1:142684/149‑1 (MQ=255)
                        cATACCAGCATCATGGCCAGCCCTCCCCAAAATCCCCCTCCCCCCAGCGCAATCCCCAACGAGGCCCACAGCGCCTCCCACAGCCCGGGTTGTCCCACCCCTCCCGCCAACGGGCGTTTTCCCCCACCGACGATCACCGCCAGTAAc                                                                                                                     <  1:170806/147‑1 (MQ=255)
                          aaccagcaTCATGTTCAGCCCTCCCCACAATCCCCCTCCCCCCAGCACAATACGCAACGAGGCCCACAGCGCCTGCCACAGCCCCGGTTGGGCCAGCACTTCCGGGAACTGGCGATTTACCCCACCGACGATCACCGCCAGTAACGGTg                                                                                                                 <  1:596051/146‑1 (MQ=255)
                              agcaTCAGGTCCACCCCTCCGCACAACACCCCGGCCGCCGGCGAAATCCGCAACGGGGCCCCCGGCGCCTGCCACAGCCCCGTTTGTCCCAGCCCTTCCGGCAACTGGCGATTTCCCCAACCGACGATCACCGCCAGTAACGGTGgcag                                                                                                             <  1:668834/149‑1 (MQ=255)
                                catcatGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAc                                                                                                         >  2:253612/1‑151 (MQ=255)
                                catcatGGTCAGCACTACGCAAAATCCCCCTGCCGCCAGCGCAATACGCAACGAGGCCCACAGCGCCTGCCACAGCCCCGGTTGGGCCAGCACTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGgc                                                                                                               <  2:252081/145‑1 (MQ=255)
                                 atcatGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAc                                                                                                         >  2:604138/1‑150 (MQ=255)
                                   catGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAcagc                                                                                                      >  2:377142/1‑151 (MQ=255)
                                      ggTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAcagcagc                                                                                                   >  2:623749/1‑151 (MQ=255)
                                          accACTCCGCAAAATCCCCCCCCCGCCAGCCCAATCCGAAAGGAGCTCCACAGCCCCTGCCACGCCACGGGTGGCGCCAGCCCTTCGGGCAACTGGCGATTTCCCCCATCTACCATCACCGCCAGTAACGGTGGCAGCAACagcagcagc                                                                                                <  2:754417/148‑1 (MQ=255)
                                            cACTCCGCACAATCCCCCTGCCGCCAGCGCAATCCGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTCCCAGCACTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGcc                                                                                             <  2:496541/151‑1 (MQ=255)
                                                 cccacaATCCCCCCGCCCCCAGCGCAATACGCAAGGGGGCCCACGGCGCCTCCCACACCCCCGGTTGCGCCAGCCCTCCCGGCAACGGGCGATTCCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGc                                                                                          <  1:851871/147‑1 (MQ=255)
                                                 cccaaaatcccccTGCCCCCGCCGCAATCCGCAACGAGTCCCACAGCCCCTGCCACAGCCCCGGTTGTGCCAGCCCTTCCGGCAACGGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAg                                                                                           <  1:262417/137‑1 (MQ=255)
                                                    acaATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAt                                                                                     >  2:441491/1‑151 (MQ=255)
                                                     caATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAtt                                                                                    >  2:1020827/1‑151 (MQ=255)
                                                       aTACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTa                                                                                   >  2:4859/1‑150 (MQ=255)
                                                              tGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCgtg                                                                           >  1:796346/1‑151 (MQ=255)
                                                                 cgccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCg                                                                        >  2:282604/1‑151 (MQ=255)
                                                                   ccAGCGCAATCCGCAACGAGGCCCACAGCGCCTGCCACAGCCCCGGTTGCGCCAGCCCTTCCGGCACCTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGc                                                                       <  2:268686/150‑1 (MQ=255)
                                                                         cAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCg                                                                >  1:275485/1‑151 (MQ=255)
                                                                          aacccGCAACGAGGCCCCCGGCGCCTGCCCCAGCCCCGGTTGTGCCAGCCCTTCCGGCAACTGGCGTTTTCCCCCATCAACGACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCgg                                                               <  2:165060/147‑1 (MQ=255)
                                                                          aaaacaaaaCGAGGCCCACAGCGCCGGCCACAGCGCCGTTGGTGCCAGCTTTTCCGGCAACGGGAAATTTACCCCATCGCCGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGc                                                                       <  1:580514/136‑1 (MQ=255)
                                                                           aTACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGc                                                              >  1:949122/1‑151 (MQ=255)
                                                                              cGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTAt                                                           >  2:98832/1‑151 (MQ=255)
                                                                              cGCAACGAGGCCCCCAGCCCCTGCCACAGCCCCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTa                                                            <  2:462921/150‑1 (MQ=255)
                                                                                 acggAGGCCCCCGGCCCCTCCCACAGCCCCGGTTGTGCCAGCACTCCCGGCAGCGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTAt                                                           <  1:770394/145‑1 (MQ=255)
                                                                                 aaCGAGGCCCACGGCGCCTGCCACACCCCCGGTTGGCCCAGCCCTCCCGGCACCGGGCGATTTCCCCCATCGCCGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTAt                                                           <  1:91760/148‑1 (MQ=255)
                                                                                  aCGGGGCCCACCGCCCCTGCCACACCACCGGTTGTCCCAGCCTTCCCGTCAACTGGGGATTTCCCCCACCGCCGATCACCGCCAGTAACGGTTGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCa                                                        <  2:218147/150‑1 (MQ=255)
                                                                                     aGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACg                                                    >  2:179638/1‑151 (MQ=255)
                                                                                      ggcccACAGCGCCTGCCACAGCCCCGGTTGTGCCAGCCCTCCCGGAAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAg                                                       <  2:597664/144‑1 (MQ=255)
                                                                                      ggcccACAGCGCCTGCCACAGCACCGGTTGTGCCAGCCCTTCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAg                                                       <  2:535258/144‑1 (MQ=255)
                                                                                         ccagccccccTGCACCAGCACTGTTTGTCCCAGCACTGCGGGCAGCTGGCGATTTACCCCACCGAGAATCTCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCg                                                <  2:801396/143‑1 (MQ=255)
                                                                                         ccACAGCGCCTCCCACAGCCCCGGTTGTGCCAGCCCTCCCGGCAACTGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCg                                                <  1:253594/151‑1 (MQ=255)
                                                                                          cacgGCCCCTGCCACAGCCCCGGTTGTGCCCGCCCTCCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGt                                               <  1:949417/151‑1 (MQ=255)
                                                                                                 ccTGCCCCAGCCCCGGTTGTGCCAGCCCTCCCGGCAACTGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCg                                            <  1:129333/147‑1 (MQ=255)
                                                                                                 ccTGCCACAGCACCGGTTGGGCCAGCACTCCCGGCAACGGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTc                                        <  2:121410/151‑1 (MQ=255)
                                                                                                    gCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTcgcg                                     >  2:1009545/1‑151 (MQ=255)
                                                                                                     ccACAGCACCGGTTGTGCCAGCACTCCCGGCAACGGGCGATTTACCCCAGCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTcg                                       <  1:441457/148‑1 (MQ=255)
                                                                                                      cacaGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGcc                                   >  2:61467/1‑151 (MQ=255)
                                                                                                         acccccGGTTGTGCCAGCCCTCCCGGCAACTGGCGATTTCCCCCACCGCCGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAg                                 <  2:240008/146‑1 (MQ=255)
                                                                                                          gCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGcc                               >  2:461119/1‑151 (MQ=255)
                                                                                                             ccGGTTGTCCCAGCACTTCCGGCAACGGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCtt                             <  2:837752/150‑1 (MQ=255)
                                                                                                                  tgggCCAGCCCTCCCGGCAACGGGCGTTTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcagc                        <  1:377115/147‑1 (MQ=255)
                                                                                                                       cAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtg                  >  1:374777/1‑151 (MQ=255)
                                                                                                                        aGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtgg                 >  2:413287/1‑151 (MQ=255)
                                                                                                                          ccctcccGGCACCGGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtggtg               <  1:407509/146‑1 (MQ=255)
                                                                                                                           aCTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGc              >  2:205304/1‑151 (MQ=255)
                                                                                                                            cTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGcc             >  1:3692/1‑151 (MQ=255)
                                                                                                                               ccGGCAACTGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCgg           <  2:740043/150‑1 (MQ=255)
                                                                                                                               ccGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCggg          >  2:73948/1‑151 (MQ=255)
                                                                                                                               ccGGCAACGGGCGTTTTCCCCCATCGACAACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCggg          <  2:948412/151‑1 (MQ=255)
                                                                                                                                 ggCACCGGGCGTTTCACCCCGGCGACGTTCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGgcg        <  2:129301/151‑1 (MQ=255)
                                                                                                                                  gCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGgcgc       >  2:800683/1‑151 (MQ=255)
                                                                                                                                   cAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGgcgc       >  2:33860/1‑150 (MQ=255)
                                                                                                                                   aatggggCGATTCCCCCCAGCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCa      <  1:282582/146‑1 (MQ=255)
                                                                                                                                       tGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATgg  >  2:603116/1‑151 (MQ=255)
                                                                                                                                        ggCGATTCCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAAt    <  2:838786/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
CGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGG  >  NC_000913/73437‑73722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: