Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	43.3%	123.1 / 12.8	67	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (34/4);  new base C (0/29);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 5.19e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTA  >  NC_000913/3443127‑3443421                                                                                                                                                         |                                                                                                                                                 gCAGACGCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTa                                                                                                                                                  <  2:171340/151‑1 (MQ=255)  cAGACGCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTAc                                                                                                                                                 <  1:695133/151‑1 (MQ=255)  cAGACGCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTAc                                                                                                                                                 >  1:166420/1‑151 (MQ=255)     aCGCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTAcccc                                                                                                                                              >  2:47658/1‑151 (MQ=255)      cGCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTAccccc                                                                                                                                             <  2:198990/151‑1 (MQ=255)       gCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCg                                                                                                                                            >  2:148859/1‑151 (MQ=255)          tAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGcc                                                                                                                                          >  2:697977/1‑150 (MQ=255)           agagTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCat                                                                                                                                        >  1:683475/1‑151 (MQ=255)                     aTAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTc                                                                                                                              >  2:863888/1‑151 (MQ=255)                      tAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTc                                                                                                                              >  2:843977/1‑150 (MQ=255)                      tAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTc                                                                                                                              >  1:589739/1‑150 (MQ=255)                        aaGCGGTTGCCCA‑GGCTGAAAATACAGCAAAATTTTTGTCAGCCAGTTCCAACCAGTCCCGCCCCCGACCCATGACGCGATGGTCGCCGGGAACAAAATAATACTGGAAGCGAAGTTTGCC‑GGGATTCCCCCCGCCATATTCACTTTCAGc                                                                                                                           <  2:945398/151‑1 (MQ=255)                         aagggTTGGCCAGGGCTAAAAATACAGCAAATTTTTTGTCGCCCAGTCCCACCCAGTCCCCCCCCCAAACAAGGCCGCGAGGGTCGCCGGAAACAAAAAAATACTGGAAGCGAAGTTGGCG‑GGGATTCCCCCCGCCATATTCACTTTCAGc                                                                                                                           <  1:566353/148‑1 (MQ=255)                            gtttGCCCA‑GGCTGAAAATACAGCAAATTTGTTGTCACCCATTCCCACCCAGTCCCGCCCCCGAACCATGCCGCGATGGTCGCCGGAAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTCCCCCCGCCATATTCACTTTCAGc                                                                                                                           <  2:612614/145‑1 (MQ=255)                            ggTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGt                                                                                                                        >  1:425802/1‑150 (MQ=255)                               tGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                    >  1:441056/1‑151 (MQ=255)                               tGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                    >  1:961346/1‑151 (MQ=255)                                gCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                   >  1:641874/1‑151 (MQ=255)                                  ccA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                 >  2:649659/1‑151 (MQ=255)                                  ccA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                 >  2:948180/1‑151 (MQ=255)                                   cA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                >  2:850701/1‑151 (MQ=255)                                      ggctaaaaaTACAGCAAAATTGTTGTCAGCCATTCCCAACCAGTCCCCCCCCCGAACCAGGACGCGGTGGTCGCCGGAAAAAAAATAATACTGGAGGCGAAGATTGCC‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                              <  2:522214/145‑1 (MQ=255)                                      ggCTGAAAATACAGCAAAATTGTTGTCAGCCAGTTCCACCCAGTCCCCCCCCCGAACCATGACGCGATGTTCGCCGGAAACAGAATAATACTGGAAGCGAAGATTGCG‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                <  2:909580/149‑1 (MQ=255)                                            aaaTACAGAAAAATTGTGGCCAGCCAGTCCCAACCATTCCCGCCCCCGAACCATGACGCGATGGTCGCGGGGAACAAAATAATACTGGAAGCGAAGATTGCG‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                        <  1:86445/151‑1 (MQ=255)                                              aTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                       >  2:56390/1‑150 (MQ=255)                                                aCAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                    >  1:827518/1‑151 (MQ=255)                                                  aGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                  >  1:724270/1‑151 (MQ=255)                                                      aattttttgtCAGCCATTTCCACCCAGTCCCGCCCCCGACCCATGACGCGATGGTCGCGGGAAAAAAAATAATACTGAAAGCGAAGATTGCG‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGa                                                                                               <  2:526839/144‑1 (MQ=255)                                                            ttgtCAGCCATTTCCAACCAGTCCCGCCCCCGAACCATGCCGCGATGGTCGCGGGGAACAAAATAATACTGGAAGCGAAGATTGCC‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                         <  2:1013949/150‑1 (MQ=255)                                                            ttgtCAGCCAGTTCCACCCAGTCCCCCCCCCGAACCATGACGCGATGGCCGCCGGAAAAAAAAAAATACTGGAAGCGAAGATTGCG‑GGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                         <  2:582598/150‑1 (MQ=255)                                                               tCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                     >  2:341032/1‑151 (MQ=255)                                                                cAGCCAGTTCCACCCATTCCCCCCCCCGAACCAGGCCGGGAGGGCCGCCGGAAACAAAATAATACTGGAAGCGAATTTTGCG‑GGTATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                     <  1:501803/150‑1 (MQ=255)                                                                cAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                    >  2:535908/1‑151 (MQ=255)                                                                 aGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                   >  1:704426/1‑151 (MQ=255)                                                                   cctttccaaaCCACTCCCCCCCCCAACCCGTGACGCGATGGTCGCCGGGAACAAAATAATCCTGGAAGCAAAGATTGCG‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                         <  2:770633/135‑1 (MQ=255)                                                                       ttCCACCCAGTCCCGCCCCCGAACCATGACGCGTTGGTCGCGGAGAACAAAATAAGGCGGGAAGCGAAGTTGGCG‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                         <  1:837106/139‑1 (MQ=255)                                                                       ttCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                             >  1:907542/1‑151 (MQ=255)                                                                         ccACCCAGCCCCCCCCCCGACCCATGACGCGATGGTCGCCGGAAAAAAAAAAATACTGGAAGCGAAGATTGCC‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                           <  2:1007940/151‑1 (MQ=255)                                                                         ccAACCAGCCCCCCCCCCGAACCATGACGCGATGGTCGCGGGGAAAAAAATAATACTGGAAGCGAAGATTGCG‑GTGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                            <  1:661901/150‑1 (MQ=255)                                                                          cAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                          >  2:779331/1‑151 (MQ=255)                                                                           aaCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                         >  1:507949/1‑151 (MQ=255)                                                                            aCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                        >  1:744604/1‑151 (MQ=255)                                                                               aGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                     >  1:951513/1‑151 (MQ=255)                                                                                    cGCCCCCGAACCATGACGCGATGGTCGCGGGGAAAAGAATAATACTGGAAGCGAAGATTGCG‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                <  2:36064/151‑1 (MQ=255)                                                                                     gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                               >  1:988618/1‑151 (MQ=255)                                                                                     gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                               >  2:81029/1‑151 (MQ=255)                                                                                        cccGACCCATGACGCGATGGTCGCCGGGAACAGAAAAATACTGGAAGCAAAGATTGCG‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                            <  2:411403/151‑1 (MQ=255)                                                                                               cATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                     >  2:455244/1‑151 (MQ=255)                                                                                                  ggcgcgATGGTCGCGGGAAACAAAATAATACTGAAAGCGAAGATTGCG‑GGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                  <  1:805809/149‑1 (MQ=255)                                                                                                  gACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                  >  2:909948/1‑151 (MQ=255)                                                                                                     gcgATGGTCGCGGGGAACAAAAAAATACTGGAAGCGAAGATTGCG‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                 <  2:166430/149‑1 (MQ=255)                                                                                                     gcgATGGTCGCCGGAAACAAAATAATACGGGAAGCAAAGATTGCC‑GGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                               <  1:343224/151‑1 (MQ=255)                                                                                                       gATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                              >  1:637374/1‑150 (MQ=255)                                                                                                                 ggaaaaaaaataatACTGGAAGCGAAGATTGCC‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                    <  2:38721/142‑1 (MQ=255)                                                                                                                 ggaaaaaaaataatACGGGAAGCGAAGATGGCC‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                   <  1:717261/143‑1 (MQ=255)                                                                                                                  ggAACAAAATAATACGGAAAGCGAAG‑TTGCCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                  <  2:613391/151‑1 (MQ=255)                                                                                                                    aaCAAAATATGACTGGAAGCGAAGATTGCC‑GGTATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                   <  1:227884/148‑1 (MQ=255)                                                                                                                    aaCAAAATAATACGGGAAGCAAAGATTGCG‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                <  2:760857/151‑1 (MQ=255)                                                                                                                                caggaaggaaAGTTTGCG‑GAGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                      <  2:314363/147‑1 (MQ=255)                                                                                                                                  ggAAGGGAATATGGCG‑GGGTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGa                    <  1:47652/149‑1 (MQ=255)                                                                                                                                  ggAAGCGAAGATTGCC‑GGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                   <  2:146369/150‑1 (MQ=255)                                                                                                                                  ggAAGCGAAGATGGCC‑GGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                  <  1:879407/151‑1 (MQ=255)                                                                                                                                    aaGCGAAGATTGCC‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                <  2:224643/151‑1 (MQ=255)                                                                                                                                        gAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt            >  2:829495/1‑151 (MQ=255)                                                                                                                                            aTTGCC‑GGGATTACGGCCGCAATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGAAGACCTTGCTGACGTTTCGCGTAGTTTAGCACAATGCGGAGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa         >  1:22064/1‑150 (MQ=255)                                                                                                                                             ttGCC‑GGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc          <  2:1020537/148‑1 (MQ=255)                                                                                                                                               gCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat      >  1:599189/1‑150 (MQ=255)                                                                                                                                                   gggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatacta  <  1:649419/151‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                 GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCC‑GGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTA  >  NC_000913/3443127‑3443421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑