Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,635,159 C→T G51D (GGC→GAC)  rob ← right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,635,1590CT98.4% 232.4 / ‑3.9 61G51D (GGC→GAC) robright oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (35/25);  total (35/26)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGAT  >  NC_000913/4635015‑4635306
                                                                                                                                                |                                                                                                                                                   
gTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAAt                                                                                                                                                 >  2:1008946/1‑149 (MQ=255)
  ctggaaaaaCTCCTTTTTAAATGCGCGGTTAAATGCCTTTTGAAATTCAAAGCGTTATTGCACCACTATCTCAAAAATCGGACCCCCATTCAGGCTTACTCCAACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCa                                                                                                                                                   <  1:303201/139‑1 (MQ=255)
  cTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGc                                                                                                                                              >  1:248416/1‑150 (MQ=255)
   tGGGCAAACGGCTTCTTGAATGCGGGGGTAAATGTCTGTTGAGAGTCGAAGGGGTATGGCAGCGCGATGTCCAGAGTCGGACGCGTAAACGGGCGTATTGCGCCCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATa                                                                                                                                                <  2:529479/147‑1 (MQ=255)
    gggCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCa                                                                                                                                             <  2:862776/149‑1 (MQ=255)
    gggCAAACTGCTTCTTAAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTCCAGCGCGATGTCCAAAATCGGCCGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGc                                                                                                                                              <  1:200871/148‑1 (MQ=255)
    gggAAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATg                                                                                                                                           <  2:980976/151‑1 (MQ=255)
      gCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGc                                                                                                                                         >  2:826035/1‑151 (MQ=255)
      gCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGc                                                                                                                                         >  1:702560/1‑151 (MQ=255)
               ttcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGt                                                                                                                                     <  2:202193/146‑1 (MQ=255)
               ttcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACAt                                                                                                                                >  1:640780/1‑151 (MQ=255)
                tcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACa                                                                                                                                 <  2:9894/149‑1 (MQ=255)
                tcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACa                                                                                                                                 <  2:388430/149‑1 (MQ=255)
                   tGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCttt                                                                                                                            >  1:759389/1‑151 (MQ=255)
                     aaTGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCttt                                                                                                                            <  2:81752/149‑1 (MQ=255)
                       tGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAAc                                                                                                                        >  2:369484/1‑151 (MQ=255)
                          gcgGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGGGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACAtt                                                                                                                     <  2:981081/151‑1 (MQ=255)
                              gTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTctct                                                                                                                 >  1:233481/1‑151 (MQ=255)
                               tAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTg                                                                                                                >  2:15120/1‑151 (MQ=255)
                                aaaTGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGAGGCCCAGAATCGGACGCGCAGTCGGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGt                                                                                                               <  1:998829/151‑1 (MQ=255)
                                 aaTGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGt                                                                                                               >  1:626557/1‑150 (MQ=255)
                                  aTGTCTGTGGAGGGCCGAGGGGGTATTGCAGCGCGATGTCAAGAATGGGACGGGTGGTCAGGGGTAGGGCTACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTa                                                                                                              <  1:1022614/150‑1 (MQ=255)
                                    gTCTGTTGAGAGTCAAAGCGGTATTGCAGCGCGATGTCCAGAACCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAg                                                                                                            <  1:513344/150‑1 (MQ=255)
                                      cTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGc                                                                                                         >  2:117659/1‑151 (MQ=255)
                                         ttGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGcc                                                                                                        <  2:929598/149‑1 (MQ=255)
                                                 cGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAAt                                                                                               >  2:593207/1‑150 (MQ=255)
                                                  gAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGTGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATa                                                                                              >  2:605180/1‑150 (MQ=255)
                                                   aaGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATa                                                                                              <  2:143852/149‑1 (MQ=255)
                                                       ggTATTGCGGCGGGATGTCCAGAATCGGACGCGCAGCCGGTCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTg                                                                                         <  2:358473/150‑1 (MQ=255)
                                                       ggTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGc                                                                                        >  2:665727/1‑151 (MQ=255)
                                                        gTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCt                                                                                       >  2:234241/1‑151 (MQ=255)
                                                         tATGGCAGCGCGATGCCCAGAATCGGACGCGCAGTCAGGCGTGGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCt                                                                                       <  1:963902/150‑1 (MQ=255)
                                                             gCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTcgc                                                                                  >  1:342316/1‑151 (MQ=255)
                                                             gCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTcgc                                                                                  >  2:604045/1‑151 (MQ=255)
                                                                  gcgATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCt                                                                              >  1:855599/1‑150 (MQ=255)
                                                                    gATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTAc                                                                            >  2:551352/1‑150 (MQ=255)
                                                                       gTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACAtt                                                                         <  2:820894/150‑1 (MQ=255)
                                                                         ccAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTc                                                                      >  2:344373/1‑151 (MQ=255)
                                                                          cAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                                                                     >  2:393614/1‑151 (MQ=255)
                                                                           aaaaTCGGACGCGCAGTCGGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                                                                     <  1:874413/148‑1 (MQ=255)
                                                                                cGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGAc                                                               >  1:830548/1‑151 (MQ=255)
                                                                                   aCGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAgg                                                            >  1:225346/1‑151 (MQ=255)
                                                                                     gcgcAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAgggg                                                          >  1:931855/1‑151 (MQ=255)
                                                                                        cAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTg                                                       >  2:447241/1‑151 (MQ=255)
                                                                                         aGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTg                                                       <  1:645214/150‑1 (MQ=255)
                                                                                          gTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGAt                                                     <  2:465460/151‑1 (MQ=255)
                                                                                           tCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATc                                                    >  2:917340/1‑151 (MQ=255)
                                                                                                   ggTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGa                                             <  2:405472/149‑1 (MQ=255)
                                                                                                      gggACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCt                                          <  2:687354/148‑1 (MQ=255)
                                                                                                          ccgccgATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTcca                                      <  1:917250/150‑1 (MQ=255)
                                                                                                             ccgATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTccagcc                                   >  2:1009692/1‑150 (MQ=255)
                                                                                                                      aCAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTaaa                           >  2:533982/1‑149 (MQ=255)
                                                                                                                       cAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAgg                        >  2:887321/1‑151 (MQ=255)
                                                                                                                           cgacgaGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgc                    <  2:201294/151‑1 (MQ=255)
                                                                                                                            gacgaGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgcg                   >  2:990819/1‑151 (MQ=255)
                                                                                                                               gaGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaat                >  2:515600/1‑151 (MQ=255)
                                                                                                                                 gCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaataa              <  1:15119/151‑1 (MQ=255)
                                                                                                                                 gCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCCAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaata               >  1:856990/1‑150 (MQ=255)
                                                                                                                                     gAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGcc          >  2:470154/1‑151 (MQ=255)
                                                                                                                                         ataCGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGcc      >  1:346595/1‑151 (MQ=255)
                                                                                                                                          taCGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCt     >  1:280983/1‑151 (MQ=255)
                                                                                                                                             gcgtCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGAt  <  2:409066/151‑1 (MQ=255)
                                                                                                                                             gcgtCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGAt  >  1:349761/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGAT  >  NC_000913/4635015‑4635306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑