Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913226,3540TA64.1% 73.3 / 166.6 131noncoding (596/2904 nt)rrlH23S ribosomal RNA of rrnH operon
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base A (51/33);  total (74/57)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 131 total aligned reads displayed.

GATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTG  >  NC_000913/226204‑226442
                                                                                                                                                      |                                                                                        
gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGA‑GGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        <  2:168950/151‑1 (MQ=11)
 aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAATCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        <  1:210493/151‑1 (MQ=11)
    gTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAg                                                                                      <  2:308144/151‑1 (MQ=31)
    gTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAATCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGtgg                                                                                      <  1:517567/150‑3 (MQ=11)
      gAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCa                                                                                    <  2:444366/151‑1 (MQ=31)
       aaCCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAg                                                                                  <  2:145488/151‑1 (MQ=11)
       aaCCAGTACAGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGcg                                                                                    <  2:177088/149‑2 (MQ=11)
        aCCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAg                                                                                  <  2:119309/151‑1 (MQ=31)
         ggaGTACAGTGAGGGAAAGGAGAAAAGAAACCAGGAGAGGGGAGTGAAATAGAACCTGAAACAGTATGAGTACAAGCAGTGGGAGCCTAGTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                                                     >  1:102381/3‑146 (MQ=2)
         ccAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        >  1:158714/1‑143 (MQ=11)
          cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGt                                                                                >  2:544075/1‑151 (MQ=31)
          cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        >  1:232556/1‑142 (MQ=11)
          cAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCtt                                                                               >  1:140435/1‑151 (MQ=11)
            gTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTa                                                                              <  2:793807/151‑1 (MQ=31)
              aCCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTaa                                                                             <  1:788509/150‑1 (MQ=31)
               ccGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAAcc                                                                           >  1:62081/1‑151 (MQ=31)
                 gTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCg                                                                          >  2:684611/1‑150 (MQ=31)
                 gTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGa                                                                         <  1:312514/151‑1 (MQ=31)
                 gTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGa                                                                         <  2:686149/151‑1 (MQ=31)
                  tGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGa                                                                         >  2:526108/1‑150 (MQ=31)
                   gAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGaa                                                                        <  2:572705/150‑1 (MQ=31)
                   gAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAAt                                                                       >  1:50583/1‑151 (MQ=31)
                    gaggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                                                     >  1:375634/3‑135 (MQ=11)
                    gaggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                                                     <  2:375677/133‑1 (MQ=11)
                     gggAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAAt                                                                       >  2:903027/1‑149 (MQ=31)
                      ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGtg                                                                                       >  1:75039/1‑130 (MQ=11)
                      ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGtg                                                                                       <  2:75044/131‑2 (MQ=11)
                      ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAg                                                                                  >  2:82262/1‑136 (MQ=11)
                        aaaGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAgggg                                                                  >  1:647575/1‑151 (MQ=31)
                          aGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAgggg                                                                  >  2:691459/1‑149 (MQ=31)
                           ggCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAggc                                                              >  2:52429/1‑149 (MQ=11)
                            gCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCa                                                                                    >  2:318831/1‑128 (MQ=11)
                            gCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCa                                                                                    <  1:318795/128‑1 (MQ=11)
                             cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCgt                                                            >  2:285529/1‑150 (MQ=11)
                             cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCgt                                                            >  1:356876/1‑150 (MQ=11)
                              gAAAGGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTGGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCgt                                                            >  1:2332/1‑149 (MQ=2)
                              gAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCg                                                             >  1:412868/1‑150 (MQ=31)
                                    aaCCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGaa                                                      <  1:389138/151‑1 (MQ=31)
                                      ccccGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAAc                                                    >  1:380350/1‑151 (MQ=31)
                                        ccGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGaaa                                                     <  1:894013/148‑1 (MQ=31)
                                         cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCg                                                  >  1:304536/1‑149 (MQ=2)
                                          ggagAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGt                                               >  1:22825/4‑151 (MQ=2)
                                          ggCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCg                                                  >  1:375737/1‑149 (MQ=31)
                                          ggCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAg                                                <  1:290307/151‑1 (MQ=31)
                                             gAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCt                                             >  1:443250/1‑151 (MQ=31)
                                                gggAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        >  2:433298/1‑104 (MQ=11)
                                                gggAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        <  1:433250/104‑1 (MQ=11)
                                                gggAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTa                                           >  1:971769/1‑150 (MQ=31)
                                                 ggAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                         >  1:843082/1‑149 (MQ=2)
                                                  gAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACt                                        <  1:443573/151‑1 (MQ=31)
                                                   aGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTg                                       <  1:445596/151‑1 (MQ=31)
                                                    gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        >  1:490386/1‑100 (MQ=11)
                                                    gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        <  2:490438/100‑1 (MQ=11)
                                                    gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     <  2:356917/151‑6 (MQ=2)
                                                    gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  2:497025/1‑146 (MQ=2)
                                                    gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  2:464183/1‑146 (MQ=2)
                                                     tGAAAAAGAACCTGAAACCGTATACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTggg                                     >  2:229587/1‑151 (MQ=14)
                                                      gAAATAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                         <  1:437947/145‑2 (MQ=2)
                                                      gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    <  2:304572/150‑1 (MQ=2)
                                                      gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCg                                   <  2:843174/151‑1 (MQ=2)
                                                      gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAACAGGGc                                    <  1:64412/150‑1 (MQ=2)
                                                       aaaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATTTTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCg                                   >  2:433324/1‑151 (MQ=14)
                                                        aataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        <  2:327888/93‑1 (MQ=11)
                                                        aataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        >  1:327852/4‑96 (MQ=11)
                                                        aaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGt                                  >  1:733558/1‑151 (MQ=31)
                                                         ataGAACCTGAAACCGTATGCGTACAAGCTGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGttt                                >  1:458434/3‑150 (MQ=2)
                                                         ataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTGAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                >  1:575716/3‑135 (MQ=2)
                                                         ataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTGAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                <  2:575776/133‑1 (MQ=2)
                                                         ataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTACAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGttt                                >  2:278231/3‑150 (MQ=2)
                                                          taGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGttt                                >  1:347937/2‑149 (MQ=2)
                                                          aaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTa                                <  2:162931/151‑1 (MQ=31)
                                                           aGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGttt                                >  2:422283/1‑148 (MQ=2)
                                                               ccTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTg                           <  1:536750/151‑1 (MQ=31)
                                                                cTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    >  2:531569/1‑140 (MQ=2)
                                                                cTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGct                         >  2:294245/1‑150 (MQ=2)
                                                                  gAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGc                          >  1:604545/1‑149 (MQ=31)
                                                                  gAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTgg                       <  1:294211/151‑1 (MQ=2)
                                                                  gAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTgg                       >  1:417148/1‑151 (MQ=2)
                                                                    aaCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAggg                      <  2:3181/151‑1 (MQ=31)
                                                                     aCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGt                     <  2:50588/151‑1 (MQ=31)
                                                                      ccGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGTGAGTCTTAATAGGGc                                    <  1:183401/134‑1 (MQ=2)
                                                                      ccGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGTGAGTCTTAATAGGGc                                    >  2:183415/1‑134 (MQ=2)
                                                                       cgtacgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    <  1:398350/127‑1 (MQ=2)
                                                                       cGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGtat                   <  2:749835/151‑1 (MQ=31)
                                                                         tatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                         <  1:209832/122‑2 (MQ=2)
                                                                         tatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                         >  2:209848/5‑125 (MQ=2)
                                                                         tatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGtat                   >  1:413846/5‑148 (MQ=2)
                                                                          gtacgtacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGGATAg                 <  2:343914/150‑1 (MQ=14)
                                                                          atgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCTTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    >  2:126150/4‑130 (MQ=2)
                                                                          atgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCTTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    <  1:126140/127‑1 (MQ=2)
                                                                             cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg            >  2:440056/1‑151 (MQ=2)
                                                                             cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAACAGGGc                                    >  1:276193/1‑127 (MQ=2)
                                                                             cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAACAGGGc                                    <  2:276219/127‑1 (MQ=2)
                                                                              gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg            >  2:382346/1‑150 (MQ=2)
                                                                              gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg            >  1:406657/1‑150 (MQ=2)
                                                                                 cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGACGCTGGGTATAGACCCGAAAc        >  2:596312/1‑151 (MQ=2)
                                                                                 cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTAAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAc        >  1:649415/1‑151 (MQ=2)
                                                                                   aGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAAcc       >  1:265677/1‑151 (MQ=31)
                                                                                       gTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGt   <  1:464481/151‑1 (MQ=31)
                                                                                         gggAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTg  >  2:198619/1‑150 (MQ=31)
                                                                                                                                                      |                                                                                        
GATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTG  >  NC_000913/226204‑226442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑