Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,635,159 C→T G51D (GGC→GAC)  rob ← right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,635,1590CT98.2% 202.8 / NA 54G51D (GGC→GAC) robright oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (31/22);  minor base G (0/1);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTG  >  NC_000913/4635019‑4635304
                                                                                                                                            |                                                                                                                                                 
gggCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATg                                                                                                                                         >  2:208693/1‑151 (MQ=255)
  gCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGc                                                                                                                                       >  1:160304/1‑151 (MQ=255)
   cAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGcc                                                                                                                                      >  1:318596/1‑151 (MQ=255)
    aaaCTGCTTCTTGAATGCGCGGGTAAATGTCCGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGcc                                                                                                                                      >  1:855501/1‑150 (MQ=255)
    aaaCTGCTTCTTGAAGGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGTTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGcc                                                                                                                                      <  2:890978/150‑1 (MQ=255)
          cttcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGAc                                                                                                                                >  2:589788/1‑150 (MQ=255)
          cttcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGAc                                                                                                                                >  1:753801/1‑150 (MQ=255)
           ttcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTg                                                                                                                                  <  2:778981/147‑1 (MQ=255)
             cttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCt                                                                                                                            >  2:441053/1‑151 (MQ=255)
                 aaTGCGCGGGTAAATGTCTGTTGAGAGTCGAAGGGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCttt                                                                                                                          <  1:736410/149‑1 (MQ=255)
                 aaTGCGCGGGTAAATGTCTGTTGAGAGTCGAAGGGGTATGGCAGCGCGATGTCCAGAACCGGACGCGCAGTCAGGCATAGTGCAACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTa                                                                                                                         <  1:141048/150‑1 (MQ=255)
                 aaTGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCttt                                                                                                                          <  2:98759/149‑1 (MQ=255)
                        gggTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATtct                                                                                                                 >  1:611239/1‑151 (MQ=255)
                            aaaTGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGt                                                                                                             >  2:942543/1‑151 (MQ=255)
                            aaaTGTCTGTTGAGAGTCGAAGCGGTATGGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGGGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTctct                                                                                                               <  1:471202/149‑1 (MQ=255)
                            aaaTGTCTGTGGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGt                                                                                                             <  2:870216/151‑1 (MQ=255)
                                 tCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAg                                                                                                          <  2:842865/149‑1 (MQ=255)
                                  cTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGc                                                                                                       >  1:810934/1‑151 (MQ=255)
                                     ttGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCAc                                                                                                    >  1:863047/1‑151 (MQ=255)
                                     ttGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAAGCGGCCGCGAAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCATCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGc                                                                                                       <  1:857330/148‑1 (MQ=255)
                                      tGAGAGTGGAAGGGTTATGGCAGCGCGATGTCCAGAATCGGACCCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCa                                                                                                     <  1:445100/149‑1 (MQ=255)
                                      tGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACt                                                                                                   >  2:953861/1‑151 (MQ=255)
                                        agagTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTg                                                                                                 >  2:52513/1‑151 (MQ=255)
                                         gagTCGAGGCGATATGGCGGCGCGTTGTCCAAAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCAc                                                                                                    <  1:472785/147‑1 (MQ=255)
                                             cGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAAt                                                                                             >  1:52268/1‑150 (MQ=255)
                                               aaGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGCGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAAc                                                                                          >  1:300407/1‑151 (MQ=255)
                                                   ggTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGc                                                                                      >  2:859972/1‑151 (MQ=255)
                                                         gCAGCGCGATTTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTcgc                                                                                <  2:331465/151‑1 (MQ=255)
                                                              gcgATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCt                                                                            >  2:270911/1‑150 (MQ=255)
                                                                    tCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGt                                                                     >  1:576319/1‑151 (MQ=255)
                                                                    tCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGt                                                                     >  2:325298/1‑151 (MQ=255)
                                                                      cAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                                                                   >  1:17618/1‑151 (MQ=255)
                                                                       aGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                                                                   >  1:486252/1‑150 (MQ=255)
                                                                       aGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGa                                                                  <  1:210791/151‑1 (MQ=255)
                                                                         aaTCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGc                                                                <  1:677514/151‑1 (MQ=255)
                                                                           tCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCg                                                               >  2:240273/1‑150 (MQ=255)
                                                                             ggACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGAc                                                             >  2:298100/1‑150 (MQ=255)
                                                                                  cgcAGTCAGGCGTAGTGCGCCCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAgggg                                                        <  1:200695/150‑1 (MQ=255)
                                                                                    cAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTg                                                     >  1:965919/1‑151 (MQ=255)
                                                                                       tCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATc                                                  >  1:825096/1‑151 (MQ=255)
                                                                                          ggCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAg                                               <  2:797064/151‑1 (MQ=255)
                                                                                                    gACAGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTcc                                     >  1:514837/1‑151 (MQ=255)
                                                                                                     aCCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGTCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTcc                                     >  2:554419/1‑150 (MQ=255)
                                                                                                       cgccgATTTCGACAAACGACGAGCACGAATATACGCGGCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTccagc                                  <  1:406177/151‑1 (MQ=255)
                                                                                                          cgATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTccagcc                                 >  1:60994/1‑149 (MQ=255)
                                                                                                            aTTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGAt                             <  2:768667/151‑1 (MQ=255)
                                                                                                              ttCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGAtt                            <  1:850514/150‑1 (MQ=255)
                                                                                                                  aCAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTaaa                         >  1:388193/1‑149 (MQ=255)
                                                                                                                                  aaTATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCg       <  2:429679/151‑1 (MQ=255)
                                                                                                                                   atatACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCgg      <  1:208678/151‑1 (MQ=255)
                                                                                                                                   atatACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCgg      >  1:64992/1‑151 (MQ=255)
                                                                                                                                       aCGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCt   <  1:52508/150‑1 (MQ=255)
                                                                                                                                       aCGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTg  <  1:643030/151‑1 (MQ=255)
                                                                                                                                       aCGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTT‑CAGCCAGATTAAAAGGGCGCGAATAATGCCGGCCTg  >  2:495050/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
GGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTG  >  NC_000913/4635019‑4635304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑