Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913226,3010GA72.9% ‑3.1 / 219.5 255noncoding (543/2904 nt)rrlH23S ribosomal RNA of rrnH operon
Reads supporting (aligned to +/- strand):  ref base G (34/35);  new base A (104/82);  total (138/117)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 255 total aligned reads displayed.

GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTG  >  NC_000913/226154‑226442
                                                                                                                                                   |                                                                                                                                             
gTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCtt                                                                                                                                            >  2:946873/1‑151 (MQ=31)
 tCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAATAACCTGAAACCGTGTACGTTCAAGCAGTGGGAGCATGCtt                                                                                                                                            <  1:642308/150‑1 (MQ=14)
    gAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGc                                                                                                                                        <  2:45158/151‑1 (MQ=31)
      atatGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCgt                                                                                                                                      >  2:802624/1‑151 (MQ=31)
           gggggACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACttgttaggtgact                                                                                                                                 >  1:402569/1‑140 (MQ=1)
              ggACCATCCTCCAAGGCTAAATACTCCTGACTGACCGCTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTg                                                                                                                               >  1:331636/1‑150 (MQ=14)
              ggACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGc                                                                                                                              <  1:384307/151‑1 (MQ=31)
                  cATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTAcc                                                                                                                         >  1:10917/1‑151 (MQ=11)
                   aTCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTAcc                                                                                                                         >  1:550029/1‑151 (MQ=31)
                   aTCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTAcc                                                                                                                         >  2:140110/1‑151 (MQ=31)
                      ctccAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGTAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTTGGTGACTGCGTACCtttt                                                                                                                     >  1:688069/1‑151 (MQ=11)
                         cAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGt                                                                                                                   >  1:466801/1‑150 (MQ=11)
                           aGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTGCCTTTTGtat                                                                                                                 >  1:838642/1‑150 (MQ=11)
                                 aaTACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATggg                                                                                                           <  1:284864/151‑1 (MQ=31)
                                 aaTACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATggg                                                                                                           <  1:185122/151‑1 (MQ=31)
                                       actgactgaCCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCg                                                                                                     >  1:412146/2‑150 (MQ=11)
                                        ctgactgaCCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCg                                                                                                     >  2:762095/1‑150 (MQ=31)
                                          gactgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTg                                                                                                                    >  2:478084/1‑132 (MQ=11)
                                              gacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTtat                                                                                               <  1:802542/150‑1 (MQ=31)
                                              gacCGATAGTGAACAAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat                                                                                             >  2:380951/1‑151 (MQ=11)
                                                ccGATAGTGAACCAGTACCGTGAGGGGAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTc                                                                                           >  1:438711/1‑151 (MQ=11)
                                                ccGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTc                                                                                           >  1:652528/1‑151 (MQ=11)
                                                 cGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATgg                                                                                                            <  2:209712/133‑1 (MQ=11)
                                                  gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                         <  2:466848/151‑3 (MQ=11)
                                                  gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                         <  2:403944/151‑3 (MQ=11)
                                                  gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                         <  2:10919/151‑3 (MQ=11)
                                                  gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGT‑AAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        <  2:400257/151‑1 (MQ=11)
                                                   aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTg                                                                                         <  2:457983/151‑1 (MQ=31)
                                                   aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAA‑CCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                         <  1:210580/149‑3 (MQ=11)
                                                          aCCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAg                                                                                  <  2:560894/151‑1 (MQ=31)
                                                           ccAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                         >  2:503214/1‑140 (MQ=11)
                                                              gTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTa                                                                              <  1:334912/151‑1 (MQ=31)
                                                               tACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTa                                                                              >  2:702895/1‑150 (MQ=31)
                                                                 ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        <  2:392682/137‑1 (MQ=11)
                                                                 ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        >  1:392648/1‑137 (MQ=11)
                                                                     gAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGAACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                         <  1:559059/132‑3 (MQ=11)
                                                                        ggAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAgg                                                                    >  1:226132/1‑151 (MQ=31)
                                                                        ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                                                         >  2:51208/1‑113 (MQ=11)
                                                                        ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTc                                                                                                         <  1:51205/113‑1 (MQ=11)
                                                                        ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAggg                                                                   >  1:177381/1‑151 (MQ=11)
                                                                             ggCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                         >  2:440659/1‑122 (MQ=11)
                                                                               cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAAt                                                                                                              >  1:483217/1‑101 (MQ=11)
                                                                               cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAAt                                                                                                              <  2:483266/101‑1 (MQ=11)
                                                                               cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCg                                                                                                     <  1:383469/110‑1 (MQ=11)
                                                                               cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                         >  2:41165/1‑120 (MQ=11)
                                                                               cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        >  1:14970/1‑123 (MQ=11)
                                                                               cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCgt                                                            >  1:449045/1‑150 (MQ=11)
                                                                                 aaaaGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGa                                                            >  1:134803/1‑150 (MQ=31)
                                                                                 aaaaGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGa                                                            >  2:184176/1‑150 (MQ=31)
                                                                                 aaaaGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        >  1:504068/1‑121 (MQ=11)
                                                                                         cccGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAAcc                                                   <  2:666489/151‑1 (MQ=31)
                                                                                         cccGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTA‑AATGGGTCAGCGACTTATATTCAGTGGCAAg                                                                                  <  2:238434/118‑1 (MQ=2)
                                                                                         cccGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTA‑AATGGGTCAGCGACTTATATTCAGTGGCAAg                                                                                  >  1:238419/1‑118 (MQ=2)
                                                                                          ccGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                                                     <  2:17715/115‑1 (MQ=11)
                                                                                          ccGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGT‑GGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                <  2:412149/151‑1 (MQ=2)
                                                                                            ggagAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTca                                                                                          >  2:373285/4‑107 (MQ=11)
                                                                                                  gggAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                         >  1:368470/1‑150 (MQ=2)
                                                                                                    gAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACt                                        >  2:64389/1‑151 (MQ=31)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGGGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  1:268761/1‑146 (MQ=2)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                         <  1:814686/99‑3 (MQ=11)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                                                     <  1:484645/103‑1 (MQ=11)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                                                     >  2:484694/1‑103 (MQ=11)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCg                                                             <  1:1997/127‑1 (MQ=2)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                >  1:582393/1‑140 (MQ=2)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  1:102586/1‑146 (MQ=2)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  2:97809/1‑146 (MQ=2)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  2:161260/1‑146 (MQ=2)
                                                                                                      gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  1:500062/1‑146 (MQ=2)
                                                                                                        gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGcg                                                                                    <  2:418809/102‑2 (MQ=2)
                                                                                                        gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggacg                                   <  2:449091/151‑8 (MQ=2)
                                                                                                           ataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGct                                                                                    <  2:882780/97‑2 (MQ=2)
                                                                                                           ataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGct                                                                                    >  1:882685/3‑98 (MQ=2)
                                                                                                           ataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGCAg                                                                <  1:45748/117‑1 (MQ=2)
                                                                                                             aGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTca                                                                                          >  1:98547/1‑90 (MQ=11)
                                                                                                             aGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAg                                                                                  <  2:58097/99‑1 (MQ=11)
                                                                                                               aaCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTATGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAg                              >  2:311113/1‑150 (MQ=14)
                                                                                                                acttGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAAc                                         >  2:190045/4‑137 (MQ=2)
                                                                                                                  cTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGc                          >  1:678793/1‑150 (MQ=2)
                                                                                                                    gAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAAcc                                                                           >  2:328069/1‑99 (MQ=2)
                                                                                                                    gAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAAcc                                                                           <  1:328041/99‑1 (MQ=2)
                                                                                                                     aaaCCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    >  2:825387/1‑137 (MQ=2)
                                                                                                                      aaCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAggg                      >  1:19260/1‑151 (MQ=31)
                                                                                                                       aCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGt                     <  2:385631/151‑1 (MQ=31)
                                                                                                                         cGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGtat                   <  2:1028032/151‑1 (MQ=31)
                                                                                                                         cGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGta                    >  2:273044/1‑150 (MQ=31)
                                                                                                                            gtacgtacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAg                 <  2:550086/150‑1 (MQ=31)
                                                                                                                             tgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatagg                                      <  2:360449/125‑5 (MQ=2)
                                                                                                                               cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg            >  1:340846/1‑151 (MQ=2)
                                                                                                                                gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    >  1:255754/1‑126 (MQ=2)
                                                                                                                                gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    <  2:255769/126‑1 (MQ=2)
                                                                                                                                   cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatagg                                      <  2:842046/121‑5 (MQ=2)
                                                                                                                                   cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatagg                                      >  1:841957/1‑117 (MQ=2)
                                                                                                                                   cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAc        >  1:408887/1‑151 (MQ=2)
                                                                                                                                   cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAACGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAc        >  2:301172/1‑151 (MQ=2)
                                                                                                                                   cAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGaaa         <  2:331664/151‑1 (MQ=31)
                                                                                                                                    aaGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        <  1:489922/70‑1 (MQ=2)
                                                                                                                                    aaGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                        >  2:489972/1‑70 (MQ=2)
                                                                                                                                    aaGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAcc       >  1:420590/1‑151 (MQ=2)
                                                                                                                                        aGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAAccc      <  1:277342/149‑1 (MQ=31)
                                                                                                                                          tGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTg  >  1:949943/1‑151 (MQ=31)
                                                                                                                                                   |                                                                                                                                             
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTG  >  NC_000913/226154‑226442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑