Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	226,381	0	C	G	62.4%	76.7 / 120.2	86	noncoding (623/2904 nt)	rrlH	23S ribosomal RNA of rrnH operon
Reads supporting (aligned to +/- strand):  ref base C (18/14);  new base G (35/18);  total (54/32)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTG  >  NC_000913/226233‑226442                                                                                                                                                     |                                                              cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCgt                                                            >  1:449045/1‑150 (MQ=11) cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCg                                                             >  1:502222/1‑150 (MQ=11)  gAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGTAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGTCgt                                                            >  2:171468/1‑149 (MQ=11)   aaaaGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGa                                                            >  1:134803/1‑150 (MQ=31)   aaaaGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGa                                                            >  2:184176/1‑150 (MQ=31)         aCCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGaa                                                      >  1:463435/1‑150 (MQ=31)           cccGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAAcc                                                   <  2:666489/151‑1 (MQ=31)            ccGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCg                                                  >  1:608285/1‑151 (MQ=31)            ccGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGT‑GGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                <  2:412149/151‑1 (MQ=2)             cGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCg                                                  >  1:921466/1‑150 (MQ=31)             cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                <  1:460448/151‑1 (MQ=2)              ggagAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGGGAGt                                               >  1:413122/4‑151 (MQ=2)                 gAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCt                                             >  1:957184/1‑151 (MQ=31)                  aGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCtt                                            <  2:461930/151‑1 (MQ=31)                   ggggAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTa                                           >  1:277448/1‑150 (MQ=2)                   ggggAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCtt                                            >  2:999152/1‑150 (MQ=31)                    gggAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                         >  1:368470/1‑150 (MQ=2)                     ggAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAAc                                         >  1:839260/1‑151 (MQ=31)                     ggAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAAc                                         <  1:953607/151‑1 (MQ=31)                      gAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatag                                       <  2:48701/151‑4 (MQ=2)                      gAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACt                                        >  2:64389/1‑151 (MQ=31)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGGGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  1:268761/1‑146 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCg                                                             >  2:1997/1‑127 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCg                                                             <  1:1997/127‑1 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                >  1:582393/1‑140 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                <  2:582455/140‑1 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  1:102586/1‑146 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  1:500062/1‑146 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  2:161260/1‑146 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     <  2:402604/151‑6 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                     >  2:97809/1‑146 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCATACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCg                                                             <  1:391361/127‑1 (MQ=2)                        gTGAAATAGAACCTGAAACCGTATGCATACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCg                                                             >  2:391395/1‑127 (MQ=2)                        gTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTgg                                      <  2:747198/151‑1 (MQ=31)                         tGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTggg                                     >  1:19965/1‑151 (MQ=31)                          gAAATATAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTATCGAAAGCGAGTCTTAATAGGGc                                    <  1:396295/150‑1 (MQ=2)                          gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggacg                                   <  2:449091/151‑8 (MQ=2)                          gAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTggg                                     <  1:571886/150‑1 (MQ=31)                          gAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTggg                                     <  1:274643/150‑1 (MQ=31)                           aaaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCg                                   >  2:972596/1‑151 (MQ=31)                            aataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGtt                                 >  2:92399/4‑151 (MQ=2)                             ataGAACCTGAAACCGGATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTGCCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGGGAGTCTTAATAGGGca                                   <  2:413158/146‑2 (MQ=2)                              taGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGttt                                >  1:122522/2‑149 (MQ=2)                                 aaCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTATGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAg                              >  2:311113/1‑150 (MQ=14)                                  acttGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAAc                                         <  1:190034/134‑1 (MQ=2)                                  acttGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAAc                                         >  2:190045/4‑137 (MQ=2)                                   ccTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTg                           >  1:137449/1‑151 (MQ=31)                                   ccTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGc                          >  2:185312/1‑151 (MQ=2)                                    cTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGc                          >  1:678793/1‑150 (MQ=2)                                       aaaCCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    <  1:825302/137‑1 (MQ=2)                                       aaaCCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    >  2:825387/1‑137 (MQ=2)                                        aaCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAggg                      >  1:19260/1‑151 (MQ=31)                                         aCCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGt                     <  2:385631/151‑1 (MQ=31)                                           cgtatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGtat                   >  2:37337/7‑150 (MQ=2)                                           cGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGtat                   <  2:1028032/151‑1 (MQ=31)                                           cGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGtat                   >  2:47677/1‑151 (MQ=31)                                           cGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGta                    >  2:273044/1‑150 (MQ=31)                                             tgtACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGtat                   >  1:994466/1‑149 (MQ=31)                                              gtacgtacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAg                 <  2:550086/150‑1 (MQ=31)                                              gtacgtacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGa                <  2:307001/151‑1 (MQ=31)                                               tgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatagg                                      <  2:360449/125‑5 (MQ=2)                                               tgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatagg                                      >  1:360417/3‑123 (MQ=2)                                               tgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGAcc              >  2:520531/3‑151 (MQ=2)                                               tgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGAcc              >  1:264104/3‑151 (MQ=2)                                                 cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGTGTCTTAATATGGCGTTTAGTCGCTGGGTATAGACCCg            >  2:136539/1‑151 (MQ=2)                                                 cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCg                                   <  2:173041/128‑1 (MQ=2)                                                 cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCg                                   >  1:173032/1‑128 (MQ=2)                                                 cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg            <  2:264119/151‑1 (MQ=2)                                                 cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg            >  1:340846/1‑151 (MQ=2)                                                 cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg            >  2:443267/1‑151 (MQ=2)                                                 cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg            >  1:406865/1‑151 (MQ=2)                                                 cgtacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGAccc             <  2:409945/151‑1 (MQ=31)                                                  gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    <  2:255769/126‑1 (MQ=2)                                                  gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    >  1:255754/1‑126 (MQ=2)                                                     cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatagg                                      <  2:842046/121‑5 (MQ=2)                                                     cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatagg                                      >  1:841957/1‑117 (MQ=2)                                                     cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAc        >  1:222902/1‑151 (MQ=2)                                                     cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAc        >  1:408887/1‑151 (MQ=2)                                                     cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAACGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAc        >  2:301172/1‑151 (MQ=2)                                                     cAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGaaa         <  2:331664/151‑1 (MQ=31)                                                      aaGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    <  1:23775/122‑1 (MQ=2)                                                      aaGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                    >  2:23777/1‑122 (MQ=2)                                                      aaGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAcc       >  1:420590/1‑151 (MQ=2)                                                       aGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAAcc       <  2:564397/151‑1 (MQ=31)                                                          aGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAAccc      <  1:277342/149‑1 (MQ=31)                                                            tGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTg  >  1:949943/1‑151 (MQ=31)                                                                                                                                                     |                                                              CGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTG  >  NC_000913/226233‑226442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑