Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,635,159 C→T G51D (GGC→GAC)  rob ← right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,635,1590CT98.2% 197.5 / ‑4.1 54G51D (GGC→GAC) robright oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (31/22);  total (32/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTG  >  NC_000913/4635012‑4635304
                                                                                                                                                   |                                                                                                                                                 
ggAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCa                                                                                                                                                 >  2:426510/1‑150 (MQ=255)
        ggCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATgg                                                                                                                                        <  2:943144/151‑1 (MQ=255)
                gCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAGGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGa                                                                                                                                 <  1:911387/150‑1 (MQ=255)
                  ttcttGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACAt                                                                                                                              >  1:714579/1‑151 (MQ=255)
                      tGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCttt                                                                                                                          >  1:1001873/1‑151 (MQ=255)
                      tGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCttt                                                                                                                          >  1:726089/1‑151 (MQ=255)
                         aTGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTaa                                                                                                                        >  2:980873/1‑150 (MQ=255)
                             gcgGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACAtt                                                                                                                   >  2:307727/1‑151 (MQ=255)
                               gggTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATtct                                                                                                                 >  2:852564/1‑151 (MQ=255)
                                   aaaTGTCTGTTGAGAGCCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTctct                                                                                                               <  2:713065/149‑1 (MQ=255)
                                    aaTGTCTGTTGAGAGTCGAGGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTa                                                                                                            <  2:464364/151‑1 (MQ=255)
                                      tGTCGGTTGGGAGTCGAGGCGGTATTGCAGCGCGATTTCCAGAATCGGACGGGCAGTCAGGCGTAGTCCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTaa                                                                                                           <  1:399769/150‑1 (MQ=255)
                                        tCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTg                                                                                                        >  1:306300/1‑151 (MQ=255)
                                        tCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTg                                                                                                        >  2:625157/1‑151 (MQ=255)
                                            ttGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCAc                                                                                                    >  1:109238/1‑151 (MQ=255)
                                            ttGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCAc                                                                                                    >  1:71506/1‑151 (MQ=255)
                                                 agTCGAGGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTg                                                                                                 <  1:58456/149‑1 (MQ=255)
                                                  gTCGAGGCGTTATTGCAGGGCGATGTCCAGATTCGGCGGGGCAGTCAGGCGTAGTGGGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTg                                                                                                 <  2:776164/148‑1 (MQ=255)
                                                    tgAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAAt                                                                                             >  1:832981/2‑150 (MQ=255)
                                                    cGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAAt                                                                                             <  2:608880/150‑1 (MQ=255)
                                                     gAAGCGTTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATa                                                                                            <  2:257171/150‑1 (MQ=255)
                                                      aaGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAAc                                                                                          >  1:281916/1‑151 (MQ=255)
                                                        gCGGGATTGCAGCGCGATCGCCAGAACCGGAGGCGCAGTCAGGCTTAGTGGGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAAc                                                                                          <  1:123215/149‑1 (MQ=255)
                                                         cGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTg                                                                                       >  2:215288/1‑151 (MQ=255)
                                                                      cgATGTCCAGAATCGGCCGCGCAGTCGGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCt                                                                            <  1:888202/149‑1 (MQ=255)
                                                                             cAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                                                                   >  2:615880/1‑151 (MQ=255)
                                                                                aaccGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAg                                                                 <  1:530285/147‑1 (MQ=255)
                                                                                    ggACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGAc                                                             >  2:474688/1‑150 (MQ=255)
                                                                                      aCGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAgg                                                          >  2:995937/1‑151 (MQ=255)
                                                                                         cgcAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGc                                                       >  1:501715/1‑151 (MQ=255)
                                                                                            aGTCAGGCGTAGGGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAgggg                                                        <  1:213368/147‑1 (MQ=255)
                                                                                             gTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAgg                                                          <  1:798063/144‑1 (MQ=255)
                                                                                             gTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTg                                                     <  2:418710/149‑1 (MQ=255)
                                                                                               cAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATcc                                                 >  2:307002/1‑151 (MQ=255)
                                                                                                      aGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGAc                                          >  2:475623/1‑151 (MQ=255)
                                                                                                               gccgATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTccagcc                                 >  2:192705/1‑151 (MQ=255)
                                                                                                                 cgATTTCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTccagccag                               <  2:286660/151‑1 (MQ=255)
                                                                                                                      tCGACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTa                           >  2:269993/1‑150 (MQ=255)
                                                                                                                        gACAAACGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTaaa                         >  1:724821/1‑150 (MQ=255)
                                                                                                                           aaaCGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGt                     <  1:211909/151‑1 (MQ=255)
                                                                                                                           aaaCGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGt                     <  2:163497/151‑1 (MQ=255)
                                                                                                                            aaCGACGAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTc                    >  1:881967/1‑151 (MQ=255)
                                                                                                                             acgacgAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcg                   <  1:553818/151‑1 (MQ=255)
                                                                                                                             acgacgAGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcg                   >  2:492811/1‑151 (MQ=255)
                                                                                                                              cgacgaGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgc                  <  2:850955/151‑1 (MQ=255)
                                                                                                                               gacgaGCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgcg                 >  2:558101/1‑151 (MQ=255)
                                                                                                                                    gCACGAATATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaata             >  2:268351/1‑150 (MQ=255)
                                                                                                                                        gAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCGGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGcc        >  1:224894/1‑151 (MQ=255)
                                                                                                                                         aaTATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCg       >  2:758426/1‑151 (MQ=255)
                                                                                                                                         aaTATACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCg       <  1:426472/151‑1 (MQ=255)
                                                                                                                                          atatACGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCgg      <  2:113800/151‑1 (MQ=255)
                                                                                                                                           tataCGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGc     >  2:946894/1‑151 (MQ=255)
                                                                                                                                            ataCGCGTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTCATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGcc    >  2:1011787/1‑151 (MQ=255)
                                                                                                                                               cgcgTCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTg  >  2:502562/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTG  >  NC_000913/4635012‑4635304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑