Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913226,2990AC78.8% 90.0 / 163.5 259noncoding (541/2904 nt)rrlH23S ribosomal RNA of rrnH operon
Reads supporting (aligned to +/- strand):  ref base A (31/24);  new base C (112/92);  total (143/116)
Fisher's exact test for biased strand distribution p-value = 8.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-03

Only 100 of 259 total aligned reads displayed.

TGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCT  >  NC_000913/226153‑226446
                                                                                                                                                  |                                                                                                                                                   
tGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCt                                                                                                                                                 >  2:550187/1‑151 (MQ=31)
 gTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCt                                                                                                                                                 <  1:236677/150‑1 (MQ=31)
        tatGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCgtg                                                                                                                                         >  2:626551/1‑151 (MQ=31)
           ggggggACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACttgttaggtgac                                                                                                                                      >  2:38913/1‑141 (MQ=1)
           ggggggACCATCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACttgttaggtgac                                                                                                                                      >  2:287194/1‑141 (MQ=1)
                gACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCATTGGGAGCATGCTTATGCGTGTGACt                                                                                                                                    <  1:83909/148‑1 (MQ=14)
                    aTCCTCCAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTAcc                                                                                                                             >  1:230970/1‑150 (MQ=11)
                         ccAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGTAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCttt                                                                                                                          <  2:131165/149‑1 (MQ=14)
                         ccAAGGCTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGt                                                                                                                       <  1:178956/151‑1 (MQ=11)
                              gCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATaa                                                                                                                   <  1:97940/151‑1 (MQ=31)
                               cTAAATACTACTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTg                                                                                                                        >  2:145919/1‑144 (MQ=11)
                                  aaTACTACTGATTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCATACCTTTTGt                                                                                                                       <  1:308867/142‑1 (MQ=11)
                                  aaTACTACTGATTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCATACCTTTTGt                                                                                                                       >  2:308836/1‑142 (MQ=11)
                                           gactgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATgg                                                                                                                <  1:138823/140‑1 (MQ=11)
                                             ctgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACtt                                                                                                     >  2:404901/1‑150 (MQ=31)
                                             ctgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACtt                                                                                                     >  1:762692/1‑150 (MQ=31)
                                             ctgacCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCg                                                                                                         <  1:284444/145‑1 (MQ=11)
                                                  cGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGGACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTc                                                                                               >  2:162402/1‑150 (MQ=11)
                                                  cGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGt                                                                                                                       <  1:346384/126‑1 (MQ=11)
                                                  cGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTca                                                                                              <  2:674700/151‑2 (MQ=11)
                                                  cGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTc                                                                                               >  2:51497/1‑150 (MQ=11)
                                                   gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTATATAATGGGTCAGCGACTTATATTCt                                                                                              >  2:613353/1‑151 (MQ=14)
                                                   gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                             <  2:167361/151‑3 (MQ=11)
                                                   gATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                             <  2:252529/151‑3 (MQ=11)
                                                    aaaGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTAAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                            <  1:44144/149‑1 (MQ=11)
                                                    aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCt                                                                                              <  1:673231/150‑1 (MQ=31)
                                                    aTAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                            <  2:314693/151‑1 (MQ=11)
                                                     tAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAAGAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCg                                                                                                         >  2:401112/1‑137 (MQ=11)
                                                      aGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTa                                                                                           <  1:376508/151‑1 (MQ=31)
                                                       gTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTca                                                                                              <  2:13428/146‑2 (MQ=11)
                                                       gTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTca                                                                                              >  1:13431/1‑145 (MQ=11)
                                                        tGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAg                                                                                                           <  1:190447/132‑1 (MQ=11)
                                                        tGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAg                                                                                                           >  2:190432/1‑132 (MQ=11)
                                                        tGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAACGGGTCAg                                                                                                           >  1:404064/1‑132 (MQ=11)
                                                         gAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGc                                                                                         >  1:287926/1‑150 (MQ=31)
                                                         gAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAAt                                                                                                                  <  1:366042/124‑1 (MQ=11)
                                                         gAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAAt                                                                                                                  >  2:366004/1‑124 (MQ=11)
                                                              aGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGtt                                                                                   >  1:680750/1‑151 (MQ=31)
                                                                 aCCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAAc                                                                                <  2:68344/151‑1 (MQ=31)
                                                                  ccGTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAAt                                                                                                                  <  1:400808/115‑1 (MQ=11)
                                                                    gTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                             <  1:22365/134‑3 (MQ=11)
                                                                    gTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                            <  1:65466/135‑1 (MQ=11)
                                                                        gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCg                                                                                                         >  1:87587/1‑118 (MQ=11)
                                                                        gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCg                                                                                                         <  2:87579/118‑1 (MQ=11)
                                                                        gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCa                                                                                        >  1:387294/1‑135 (MQ=11)
                                                                        gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCa                                                                                        <  2:387254/135‑1 (MQ=11)
                                                                        gggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAgg                                                                        >  2:47835/1‑151 (MQ=11)
                                                                         ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAGATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAggg                                                                       >  2:204054/1‑151 (MQ=11)
                                                                         ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATgggc                                                                                                              >  2:231106/1‑111 (MQ=11)
                                                                         ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTAt                                                                           >  1:389409/1‑147 (MQ=11)
                                                                         ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAggg                                                                       >  2:31214/1‑151 (MQ=11)
                                                                              ggCGAAAAGAACCCCGGATAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCg                                                                                                         >  1:204587/1‑112 (MQ=11)
                                                                              ggCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATgggc                                                                                                              >  2:400500/1‑106 (MQ=11)
                                                                              ggCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGtg                                                                                           >  2:342170/1‑125 (MQ=11)
                                                                              ggCGAAAAGAACCACGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                             >  2:355386/1‑122 (MQ=11)
                                                                               gCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGcg                                                                                        <  1:255654/128‑2 (MQ=11)
                                                                                cGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCg                                                                 >  1:699674/1‑151 (MQ=31)
                                                                                cGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCg                                                                 >  1:350378/1‑151 (MQ=31)
                                                                                cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAg                                                                                                           >  2:296999/1‑108 (MQ=11)
                                                                                cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAg                                                                                                           <  1:297028/108‑1 (MQ=11)
                                                                                cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                            <  2:225908/123‑1 (MQ=11)
                                                                                 gAAAAGAACCCCGGAGAGGGGAGTGAGATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTca                                                                                              >  2:646827/1‑119 (MQ=11)
                                                                                  aaaaGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCgt                                                                >  1:312244/1‑148 (MQ=11)
                                                                                  aaaaGAACCCCGGAGAGGGGAGTGAAATAGAACCTAAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                             <  1:269464/120‑3 (MQ=11)
                                                                                    aaGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCa                                                                                        >  2:140079/1‑123 (MQ=11)
                                                                                     aGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTTAG‑‑‑‑GTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGaa                                                          >  1:53064/1‑149 (MQ=11)
                                                                                      gAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGa                                                           <  2:71230/151‑1 (MQ=31)
                                                                                            cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                             >  1:315983/1‑108 (MQ=11)
                                                                                            cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                            >  1:410354/1‑111 (MQ=11)
                                                                                            cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCggg                                                    <  1:338979/151‑3 (MQ=2)
                                                                                            cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCg                                                      >  2:338889/1‑149 (MQ=2)
                                                                                             ggagAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGTAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGt                                                   >  1:265054/4‑151 (MQ=2)
                                                                                                  ggggAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTatat                                                                                                 <  2:207869/100‑1 (MQ=11)
                                                                                                    ggAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGc                                                                                         >  2:223072/1‑106 (MQ=11)
                                                                                                       gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                             >  1:397333/1‑97 (MQ=11)
                                                                                                       gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGt                                                                                            <  1:208681/100‑1 (MQ=11)
                                                                                                       gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         <  2:528808/151‑6 (MQ=2)
                                                                                                       gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         <  1:113379/151‑6 (MQ=2)
                                                                                                       gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatag                                           >  2:272616/1‑146 (MQ=2)
                                                                                                       gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGAAGGGGAAGCGAAAACAAGCCTTaataggg                                         >  2:175094/1‑146 (MQ=2)
                                                                                                       gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGCCAGCGACTTATATTcag                                                                                             <  2:412225/99‑3 (MQ=11)
                                                                                                         gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGTAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                        <  2:265028/150‑1 (MQ=2)
                                                                                                         gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTTTATTcag                                                                                             >  1:221106/1‑95 (MQ=2)
                                                                                                         gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                        <  1:51503/150‑1 (MQ=2)
                                                                                                          aaataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGtgg                                                                                          >  1:187654/1‑97 (MQ=11)
                                                                                                          aaataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGtgg                                                                                          <  2:187641/99‑3 (MQ=11)
                                                                                                          aaataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTCTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCgg                                      <  1:92188/151‑2 (MQ=2)
                                                                                                             taGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGttt                                    >  2:205370/2‑149 (MQ=2)
                                                                                                               gAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAggg                                                                       >  2:422919/1‑113 (MQ=11)
                                                                                                               gAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAggg                                                                       <  1:422959/113‑1 (MQ=11)
                                                                                                                          catatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAg                                                                    >  1:169111/7‑105 (MQ=2)
                                                                                                                                cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTcag                                                                                             <  2:256345/74‑3 (MQ=11)
                                                                                                                                cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTGGGCGTAGCGAAAGCGAGTCTTaat                                             >  2:262569/1‑121 (MQ=2)
                                                                                                                                cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                    >  1:164570/1‑115 (MQ=2)
                                                                                                                                 gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg                >  1:110127/1‑150 (MQ=2)
                                                                                                                                 gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTAAATAGGGCGTTTAGTCGCTGGGTATAGACCCg                >  2:585480/1‑150 (MQ=2)
                                                                                                                                   acAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGa               <  2:813831/150‑1 (MQ=31)
                                                                                                                                    cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                    >  1:15036/1‑111 (MQ=2)
                                                                                                                                     aaGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGa                                                     >  2:117083/1‑109 (MQ=2)
                                                                                                                                               aGCATGCTTAGGCGTGTGACTGCGTACCTTTTATATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAGCCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCt  <  1:613408/151‑1 (MQ=14)
                                                                                                                                                  |                                                                                                                                                   
TGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCT  >  NC_000913/226153‑226446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑