Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,635,243 G→T S23* (TCG→TAG)  rob ← right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,635,2430GT100.0% 187.4 / NA 49S23* (TCG→TAG) robright oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/25);  total (24/25)

GCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTC  >  NC_000913/4635100‑4635389
                                                                                                                                               |                                                                                                                                                  
gcgcAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAggg                                                                                                                                              <  2:183027/150‑1 (MQ=255)
     gacgggCGAAGGGCGTCCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAgg                                                                                                                                               <  2:218231/140‑1 (MQ=255)
     gTCAGGCGTGGGGGGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAg                                                                                                                                                <  2:233724/143‑1 (MQ=255)
         ggCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATcc                                                                                                                                      >  2:212312/1‑149 (MQ=255)
           cGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATcc                                                                                                                                      >  1:39006/1‑147 (MQ=255)
            gTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGAt                                                                                                                                  <  1:764595/150‑1 (MQ=255)
              aGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGAc                                                                                                                               >  1:188806/1‑151 (MQ=255)
                 gCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCt                                                                                                                             <  1:550830/150‑1 (MQ=255)
                           aTTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGAt                                                                                                                  <  2:370253/151‑1 (MQ=255)
                              tCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTa                                                                                                                <  1:243824/150‑1 (MQ=255)
                                  cAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAgg                                                                                                           >  1:280471/1‑151 (MQ=255)
                                   aaaCGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGt                                                                                                          <  2:778432/151‑1 (MQ=255)
                                     acgacgAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcg                                                                                                        <  2:453152/151‑1 (MQ=255)
                                      cgacgaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgc                                                                                                       >  1:73700/1‑151 (MQ=255)
                                             cACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaataat                                                                                                >  2:607360/1‑151 (MQ=255)
                                              aCGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATg                                                                                               >  2:486316/1‑151 (MQ=255)
                                               cGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGc                                                                                              >  2:736486/1‑151 (MQ=255)
                                                  atatACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCgg                                                                                           >  1:135662/1‑151 (MQ=255)
                                                    ataCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCTGGcc                                                                                         >  1:363818/1‑151 (MQ=255)
                                                    ataCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGc                                                                                          <  1:811031/150‑1 (MQ=255)
                                                      aCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTg                                                                                       >  1:630931/1‑151 (MQ=255)
                                                              aTAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATa                                                                                >  1:722957/1‑150 (MQ=255)
                                                              aTAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATa                                                                                >  2:247218/1‑150 (MQ=255)
                                                                  cATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAat                                                                            >  2:670954/1‑150 (MQ=255)
                                                                           tGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATcc                                                                  >  2:595352/1‑151 (MQ=255)
                                                                            gACATCTTTAAACATTCTCTTTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCt                                                                 <  2:329568/151‑1 (MQ=255)
                                                                              cATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCttt                                                               <  1:89647/151‑1 (MQ=255)
                                                                                tCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTc                                                              >  2:515072/1‑150 (MQ=255)
                                                                                     aaaCATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCaacaac                                                        <  1:730752/151‑1 (MQ=255)
                                                                                              ctGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTg                                               >  2:673246/1‑151 (MQ=255)
                                                                                                gTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTGCAACAACGAGCACCt                                                <  2:120019/148‑1 (MQ=255)
                                                                                                  aaGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACAt                                           <  1:66192/151‑1 (MQ=255)
                                                                                                  aaGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACAt                                           >  2:762875/1‑151 (MQ=255)
                                                                                                      gCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAg                                        <  1:544554/150‑1 (MQ=255)
                                                                                                      gCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAg                                        <  2:484901/150‑1 (MQ=255)
                                                                                                        cACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTa                                     <  1:475797/151‑1 (MQ=255)
                                                                                                                aTAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGata                             <  1:100403/151‑1 (MQ=255)
                                                                                                                  aaCCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGataat                           >  2:550428/1‑151 (MQ=255)
                                                                                                                   aCCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGataat                           >  2:266684/1‑150 (MQ=255)
                                                                                                                    ccTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGataat                           >  1:668284/1‑149 (MQ=255)
                                                                                                                             gccgcTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTg                <  2:39000/151‑1 (MQ=255)
                                                                                                                             gccgcTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTg                <  1:775610/151‑1 (MQ=255)
                                                                                                                                gcTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTg             >  2:202092/1‑151 (MQ=255)
                                                                                                                                gcTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTg             <  1:674072/151‑1 (MQ=255)
                                                                                                                                   aCATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGt            <  1:368655/149‑1 (MQ=255)
                                                                                                                                    cATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGtttt         >  2:431296/1‑151 (MQ=255)
                                                                                                                                    cATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGtttt         >  1:427062/1‑151 (MQ=255)
                                                                                                                                       tGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAg       <  2:458233/150‑1 (MQ=255)
                                                                                                                                           gAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTc  <  2:278665/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTC  >  NC_000913/4635100‑4635389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑