Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	226,363	0	G	C	76.1%	15.6 / 72.6	92	noncoding (605/2904 nt)	rrlH	23S ribosomal RNA of rrnH operon
Reads supporting (aligned to +/- strand):  ref base G (8/14);  new base C (44/26);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 4.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCT  >  NC_000913/226215‑226446                                                                                                                                                     |                                                                                    aGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGtt                                                                                   >  1:618267/1‑151 (MQ=31)      cGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTGCGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCg                                                                              <  2:566861/151‑1 (MQ=14)       gTGAGGGAAAGGCGAAAAGAACCCCGGAGAGGGGATTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCgt                                                                             >  2:7180/1‑149 (MQ=11)         gAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAAt                                                                           >  2:627352/1‑151 (MQ=31)           gggAAAGGCGAAAAGAACCCCGGAGAGGGGTGTGAAATAGAACCTGAAACCGTATACGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAgg                                                                        >  1:116416/1‑151 (MQ=11)            ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCgta                                                                            >  1:201287/1‑144 (MQ=11)            ggAAAGGCGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCgta                                                                            <  2:201304/146‑3 (MQ=11)             gAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAggg                                                                       >  1:738773/1‑151 (MQ=31)               aaGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGa                                                                     <  2:616678/151‑1 (MQ=31)               aaGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGa                                                                     <  2:331502/151‑1 (MQ=31)                   cGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCg                                                                 <  1:315836/151‑1 (MQ=31)                   cGAAAAGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCgt                                                                >  1:284502/1‑150 (MQ=11)                      aaaGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCGGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAgc                                                             >  1:386991/1‑150 (MQ=2)                       aaGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGGCTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAgcg                                                            <  1:404138/151‑3 (MQ=2)                       aaGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAgcg                                                            <  1:34153/151‑3 (MQ=2)                       aaGAACCCCGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAgcg                                                            >  2:34158/1‑149 (MQ=2)                             cccGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAAcc                                                       <  2:572132/151‑1 (MQ=31)                               cGGAGAGGGGAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                    <  2:284526/151‑1 (MQ=2)                                  cGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTc                                                  <  1:144668/151‑1 (MQ=31)                                   gAGGGGTGTGAAATAGAACCTGAAACCGTATACGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCtt                                                <  2:116426/151‑1 (MQ=2)                                        gAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                    <  1:266533/142‑1 (MQ=2)                                        gAGTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                    >  2:266555/1‑142 (MQ=2)                                         aGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTg                                           <  2:497193/151‑1 (MQ=31)                                          gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATGGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         >  1:31397/1‑146 (MQ=2)                                          gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAgggc                                                                      <  2:102341/122‑2 (MQ=11)                                          gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAgggc                                                                      >  1:102331/1‑121 (MQ=11)                                          gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         >  1:300295/1‑146 (MQ=2)                                          gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         >  2:207852/1‑146 (MQ=2)                                          gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         >  1:335949/1‑146 (MQ=2)                                          gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         <  2:230839/151‑6 (MQ=2)                                          gTGAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         >  1:278743/1‑146 (MQ=2)                                            gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCGGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                        <  2:387027/150‑1 (MQ=2)                                            gAAATAGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCg                                       <  1:207835/151‑1 (MQ=2)                                              aataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCg                                                                              >  2:106428/4‑110 (MQ=11)                                              aataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCg                                                                              <  1:106418/107‑1 (MQ=11)                                              aataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGtt                                     >  2:271815/4‑151 (MQ=2)                                              aaaaGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGt                                      >  2:721485/1‑151 (MQ=31)                                               ataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                        <  2:769786/145‑1 (MQ=2)                                               ataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGc                                        >  1:769692/3‑147 (MQ=2)                                               ataGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGttt                                    >  2:406054/3‑150 (MQ=2)                                                 aGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGGGCCGAAGCGAAACCGAGTCTTAACTGGGCGTTaa                                   <  1:799902/151‑1 (MQ=14)                                                 aGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTaa                                   <  2:298097/151‑1 (MQ=31)                                                 aGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATGCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTaa                                   <  2:468235/151‑1 (MQ=14)                                                 aGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTAt                                                                           <  2:249692/110‑1 (MQ=11)                                                 aGAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTAt                                                                           >  1:249670/1‑110 (MQ=11)                                                  gAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAg                                  <  2:135039/151‑1 (MQ=31)                                                  gAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGaaa                                                         <  2:391553/127‑1 (MQ=2)                                                  gAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGaaa                                                         >  1:391515/1‑127 (MQ=2)                                                  gAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         >  1:274611/1‑138 (MQ=2)                                                  gAACCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaataggg                                         <  2:274634/143‑6 (MQ=2)                                                   aaCCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGt                                 >  1:169386/1‑151 (MQ=31)                                                   aaCCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatagagcg                                       <  1:188173/144‑8 (MQ=2)                                                   aaCCTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaatagagcg                                       >  2:188189/1‑137 (MQ=2)                                                     ccTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTg                               <  2:584358/151‑1 (MQ=31)                                                      cTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGGGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGct                             >  2:55546/1‑150 (MQ=2)                                                      cTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGAGAAAGCGAGTCTTAATAGGGc                                        <  1:275827/140‑1 (MQ=2)                                                      cTGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGAGAAAGCGAGTCTTAATAGGGc                                        >  2:275850/1‑140 (MQ=2)                                                       tGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGc                              >  2:717084/1‑150 (MQ=31)                                                       tGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaa                                              <  2:181760/133‑1 (MQ=2)                                                       tGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaa                                              >  1:181744/1‑133 (MQ=2)                                                       tGAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGctg                            >  2:179184/1‑149 (MQ=2)                                                        gAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGcgg                            <  2:335978/150‑3 (MQ=2)                                                        gAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTgg                           >  1:347728/1‑151 (MQ=2)                                                        gAAACCGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTgg                           >  2:57193/1‑151 (MQ=2)                                                            ccGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaata                                            <  1:14523/130‑3 (MQ=2)                                                            ccGTATGCGTACAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaata                                            >  2:14526/1‑128 (MQ=2)                                                             cgtatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGtat                       >  1:391454/7‑150 (MQ=2)                                                             cgtatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGtat                       >  1:50430/7‑150 (MQ=2)                                                             cgtatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGtat                       >  2:385234/7‑150 (MQ=2)                                                             cgtatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGtat                       >  1:233314/7‑150 (MQ=2)                                                              gtatgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGtat                       >  2:161720/6‑149 (MQ=2)                                                                atgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                             >  2:167468/4‑124 (MQ=2)                                                                atgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                             <  1:167454/122‑2 (MQ=2)                                                                 tgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAgg                                                                   <  2:111089/100‑2 (MQ=2)                                                                 tgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAgg                                                                   >  1:111079/3‑101 (MQ=2)                                                                 tgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                             <  1:163947/122‑2 (MQ=2)                                                                 tgcgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTaat                                             >  2:163961/3‑123 (MQ=2)                                                                   cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCttgatagggc                                        <  1:222270/127‑9 (MQ=2)                                                                   cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCttgatagggc                                        >  2:222288/1‑119 (MQ=2)                                                                   cgtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg                >  2:403347/1‑151 (MQ=2)                                                                    gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCg                >  1:269979/1‑150 (MQ=2)                                                                    gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAACAgg                                          <  2:292494/124‑1 (MQ=2)                                                                    gtacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAACAgg                                          >  1:292467/1‑124 (MQ=2)                                                                     tacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                    <  1:140511/113‑1 (MQ=2)                                                                     tacAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAg                                                    >  2:140522/1‑113 (MQ=2)                                                                     tacAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCg                >  1:363719/1‑150 (MQ=31)                                                                       cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTGTAGACCCGAAAc            >  2:445424/1‑151 (MQ=2)                                                                       cAAGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAc            >  1:34326/1‑151 (MQ=2)                                                                        aaGCAGTGGGAGCCTACTT‑GTTAGGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCAGTGGCAAGCTTAACCGTATAGGGTAGGCGTAGCGAAAGCGAGTCTTAATAGGGCGTTTAGTCGCTGGGTATAGACCCGAAAcc           >  1:126601/1‑151 (MQ=2)                                                                         aGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAAcc           <  2:306295/151‑1 (MQ=31)                                                                               gggAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTg      <  2:224808/150‑1 (MQ=31)                                                                                  aGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCt  >  2:83949/1‑151 (MQ=31)                                                                                                                                                     |                                                                                    AGTACCGTGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGTGAAAAAGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCTTAGGCGTGTGACTGCGTACCTTTTGTATAATGGGTCAGCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAACCCGGTGATCT  >  NC_000913/226215‑226446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑