Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,243	G→T	S23* (TCG→TAG)	rob ←	right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,243	0	G	T	100.0%	183.8 / NA	48	S23* (TCG→TAG)	rob	right oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/25);  total (23/25)

GCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCA  >  NC_000913/4635100‑4635386                                                                                                                                                |                                                                                                                                                gcgcAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAgggg                                                                                                                                          >  2:166981/1‑151 (MQ=255)           gCGTAGGGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGa                                                                                                                                <  1:607723/151‑1 (MQ=255)             gTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATg                                                                                                                              >  1:40746/1‑151 (MQ=255)               aGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGAc                                                                                                                            >  1:461744/1‑151 (MQ=255)                gTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATg                                                                                                                              <  2:38435/148‑1 (MQ=255)                gTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGAc                                                                                                                            <  1:173412/150‑1 (MQ=255)                            aTTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGAt                                                                                                               <  2:515736/151‑1 (MQ=255)                             tttCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGAtt                                                                                                              >  1:522538/1‑151 (MQ=255)                              ttCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGAtt                                                                                                              >  2:404858/1‑150 (MQ=255)                                  aCAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAg                                                                                                         <  2:716298/151‑1 (MQ=255)                                     aaCGGCGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCTAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTc                                                                                                      <  2:674674/151‑1 (MQ=255)                                          cgaGCACGAATATACGCGCCAATAGTATAGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCGCTTGGAATAACCTGCGTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATGCAGATGACCTTCCAGCCAGATTAAAAGGTcgc                                                                                                    <  1:338056/148‑1 (MQ=255)                                          cgaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGa                                                                                                  >  1:419748/1‑150 (MQ=255)                                           gaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaa                                                                                                 <  2:390234/150‑1 (MQ=255)                                              cACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaataat                                                                                             <  2:328638/151‑1 (MQ=255)                                               aCGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATg                                                                                            <  2:459747/151‑1 (MQ=255)                                                 gAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGcc                                                                                          <  1:526133/151‑1 (MQ=255)                                                     ataCTCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAACAGGTCGCGAATGATGCCGGcc                                                                                      >  1:400524/1‑151 (MQ=255)                                                       aCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCt                                                                                     <  1:725458/150‑1 (MQ=255)                                                       aCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTg                                                                                    <  2:104113/151‑1 (MQ=255)                                                       aCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTg                                                                                    <  1:740614/151‑1 (MQ=255)                                                          cgcCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATc                                                                                 >  1:206066/1‑151 (MQ=255)                                                               aTAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATaa                                                                            <  1:14717/151‑1 (MQ=255)                                                                 aGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATaaa                                                                           >  1:358260/1‑150 (MQ=255)                                                                           gTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATc                                                                <  1:413697/151‑1 (MQ=255)                                                                            tGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATcc                                                               >  1:505846/1‑151 (MQ=255)                                                                            tGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATcc                                                               >  1:681625/1‑151 (MQ=255)                                                                             gACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCt                                                              >  2:18580/1‑151 (MQ=255)                                                                               cATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCttt                                                            >  1:552142/1‑151 (MQ=255)                                                                                     tAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTcaaca                                                       >  1:193675/1‑150 (MQ=255)                                                                                      aaaCATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCaacaac                                                     >  2:548387/1‑151 (MQ=255)                                                                                                 gTAAGTGCCGCTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCt                                             <  2:387343/148‑1 (MQ=255)                                                                                                 gTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGAc                                          >  2:112724/1‑151 (MQ=255)                                                                                                   aaGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACAt                                        >  1:365344/1‑151 (MQ=255)                                                                                                     gTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATc                                       >  1:168715/1‑150 (MQ=255)                                                                                                     gTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATc                                       >  2:522200/1‑150 (MQ=255)                                                                                                       gCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAg                                     <  2:511937/150‑1 (MQ=255)                                                                                                        ccACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGt                                   <  2:100978/151‑1 (MQ=255)                                                                                                          aCTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTaa                                 <  1:167007/151‑1 (MQ=255)                                                                                                             tGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAAtt                               <  2:333453/150‑1 (MQ=255)                                                                                                             tGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAAtt                               <  1:131695/150‑1 (MQ=255)                                                                                                              ggAATAACCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTgg                             <  2:751293/151‑1 (MQ=255)                                                                                                                  tAACCTGCTTTCGCCGCTACATTCGCGAGGTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTGCAACAACGAGCACCTGACATCAGGTAATTg                              >  2:546076/1‑146 (MQ=255)                                                                                                                   aaCCTGCTTTCGCCGCTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGataat                        >  1:176914/1‑151 (MQ=255)                                                                                                                             cgccgcTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCAtttttt              >  1:28375/1‑151 (MQ=255)                                                                                                                                ctcTACATTGTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCt           >  1:737140/3‑151 (MQ=255)                                                                                                                                         gTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCa  <  1:318577/151‑1 (MQ=255)                                                                                                                                         gTCGAGCTACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCa  <  2:60826/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                GCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCA  >  NC_000913/4635100‑4635386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑