Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,038,7440GA73.6% 54.4 / 101.8 129noncoding (1226/2905 nt)rrlA23S ribosomal RNA of rrnA operon
Reads supporting (aligned to +/- strand):  ref base G (18/16);  new base A (49/46);  total (67/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTA  >  NC_000913/4038599‑4038870
                                                                                                                                                  |                                                                                                                              
tttAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATg                                                                                                                           >  2:769768/1‑151 (MQ=39)
  tAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGc                                                                                                                          <  1:142082/150‑1 (MQ=40)
   aaagaaagCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTg                                                                                                                        <  2:696608/151‑1 (MQ=39)
   aaagaaagCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTg                                                                                                                        >  2:672552/1‑151 (MQ=39)
     agaaagCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAgg                                                                                                                    <  1:27076/151‑1 (MQ=2)
         agCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGCGGCATGCTGGAgg                                                                                                                    >  1:770351/1‑149 (MQ=255)
           cGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTAt                                                                                                                 >  1:4232/1‑150 (MQ=40)
            gTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGG‑‑GTGTCA‑TGTAAGTGACGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATc                                                                                                                >  2:336841/1‑148 (MQ=2)
               aTAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGaa                                                                                                            <  2:731368/151‑1 (MQ=39)
                     cACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGc                                                                                                        <  2:544327/149‑1 (MQ=40)
                     cACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAAt                                                                                                    <  1:561233/151‑1 (MQ=2)
                       ctagTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGc                                                                                                  >  2:30892/4‑151 (MQ=2)
                       ctagTCGAGTCGGACTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGc                                                                                                  >  1:51377/4‑151 (MQ=2)
                         agTCGAGTCGGCCTGCGCAGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTg                                                                                                <  2:2341/150‑1 (MQ=2)
                            cGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGa                                                                                               <  2:425929/151‑1 (MQ=39)
                            cGAGTCGGACTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                                                                              <  2:51393/150‑1 (MQ=2)
                              aGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAtg                                                                                           <  1:184625/151‑2 (MQ=2)
                              aGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAtg                                                                                           >  2:169971/1‑150 (MQ=2)
                               gTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAtg                                                                                           >  2:10560/1‑149 (MQ=2)
                               gTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAtg                                                                                           >  2:88690/1‑149 (MQ=2)
                                 cGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAg                                                                                         <  1:10560/150‑1 (MQ=2)
                                 cGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAg                                                                                         <  1:30882/150‑1 (MQ=2)
                                 cGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGt                                                                                        >  1:367338/1‑151 (MQ=2)
                                     cTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                                                                              <  1:360376/141‑1 (MQ=2)
                                     cTGCGCGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                                                                              >  2:360441/1‑141 (MQ=2)
                                       gcgcGGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACg                                                                                    <  1:542019/149‑1 (MQ=2)
                                       gcacgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGac                                                                                  >  2:282815/4‑150 (MQ=2)
                                        cgcgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTg                                                                                                <  1:301593/136‑1 (MQ=2)
                                        cgcgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTg                                                                                                >  1:432545/1‑136 (MQ=2)
                                        cgcgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTg                                                                                                >  2:301647/1‑136 (MQ=2)
                                        cgcgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                                                                              >  2:168781/1‑138 (MQ=2)
                                        cgcgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                                                                              <  1:168751/138‑1 (MQ=2)
                                        cgcgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACg                                                                                    >  2:208050/1‑148 (MQ=2)
                                        cgcgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACg                                                                                    <  1:208013/148‑1 (MQ=2)
                                        cgcgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGac                                                                                  >  2:141948/1‑149 (MQ=2)
                                         gcgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAg                                                                                                                     <  2:518014/114‑1 (MQ=2)
                                         gcgGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGAt                                                                                  >  1:13656/1‑151 (MQ=39)
                                          cgGAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACaat                                                                               <  1:282762/151‑2 (MQ=2)
                                          cgGAAGATGTAACGGGGCT‑AAACCATGCACAGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGGGTGCTGTGAGGCATGCTGGAGGAATCAGAAGTGCGAATGCTGACATAAGTAACGAt                                                                                  >  2:238456/1‑150 (MQ=255)
                                           ggAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCTTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                                                                              <  1:349453/135‑1 (MQ=2)
                                           ggAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCTTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                                                                              >  2:349516/1‑135 (MQ=2)
                                           ggAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGa                                                                                                                      >  2:91620/1‑111 (MQ=2)
                                           ggAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGa                                                                                                                      <  1:91600/111‑1 (MQ=2)
                                           ggAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAg                                                                                         >  1:516997/1‑140 (MQ=2)
                                            gAAGATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACaatgg                                                                             <  1:169941/151‑4 (MQ=2)
                                               gATGTAACGGGGCTCAAACCACACACCGAAGCTGCGGGTGTCAC‑GTAAGTGACG‑‑CGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACaatgggtg                                                                          <  1:69830/151‑7 (MQ=2)
                                                aTGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCgg                                                                           <  1:512380/151‑1 (MQ=39)
                                                 tGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCggg                                                                          >  2:35579/1‑151 (MQ=39)
                                                              aaaCCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGccc                                                                <  2:689120/149‑1 (MQ=40)
                                                                          gAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGAc                                                  >  1:531150/1‑151 (MQ=39)
                                                                           aaGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGAcc                                                 <  1:63389/151‑1 (MQ=39)
                                                                            aGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGAcc                                                 >  2:454227/1‑150 (MQ=40)
                                                                             gCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCa                                                >  1:124775/1‑150 (MQ=40)
                                                                                   gCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAGGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCGAGGGtt                                          <  2:486607/150‑1 (MQ=255)
                                                                                    cAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTcc                                        >  2:223002/1‑151 (MQ=39)
                                                                                             cgtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGAc                                                  <  1:268330/120‑1 (MQ=2)
                                                                                             cgtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGAc                                                  >  2:268382/13‑132 (MQ=2)
                                                                                             cgtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAggg                                            <  2:176831/126‑1 (MQ=2)
                                                                                              gtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAg                                                    <  1:311627/118‑1 (MQ=2)
                                                                                              gtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCa                                                >  1:142265/12‑133 (MQ=2)
                                                                                              gtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCa                                                <  2:142294/122‑1 (MQ=2)
                                                                                              gtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAg                                              >  2:321916/12‑135 (MQ=2)
                                                                                              gtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAg                                              <  1:321856/124‑1 (MQ=2)
                                                                                              gtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAg                                              <  2:191793/124‑1 (MQ=2)
                                                                                              gtaagtgacgcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAg                                              >  1:191760/12‑135 (MQ=2)
                                                                                              gtaagtgacgcgatAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCt                                       >  1:131581/14‑142 (MQ=2)
                                                                                              gtaagtgacgcgatAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCt                                       <  2:131609/129‑1 (MQ=2)
                                                                                                  gttgttGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTaa                           <  2:273352/150‑1 (MQ=31)
                                                                                                  gttgttGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAAt                          <  2:838595/151‑1 (MQ=31)
                                                                                                    tgttgGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATc                         <  2:475008/150‑1 (MQ=31)
                                                                                                       gcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACaatggg                                                                            <  1:172897/94‑5 (MQ=11)
                                                                                                       gcggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACaatggg                                                                            >  2:172929/3‑92 (MQ=11)
                                                                                                        cggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACg                                                                                    <  1:409245/86‑1 (MQ=11)
                                                                                                        cggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACg                                                                                    >  2:409316/2‑87 (MQ=11)
                                                                                                        cggTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACaatg                                                                              <  1:194985/92‑3 (MQ=11)
                                                                                                            agaggAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGaaa                                                     >  2:257729/4‑114 (MQ=2)
                                                                                                             gaggAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCTg                                      >  2:294182/3‑128 (MQ=11)
                                                                                                                 aGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAg            >  1:596514/1‑150 (MQ=31)
                                                                                                                  gCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGAcc                                                 <  1:94634/112‑1 (MQ=2)
                                                                                                                  gCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAgg                                             >  1:417997/1‑116 (MQ=2)
                                                                                                                  gCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAgg                                             <  2:418070/116‑1 (MQ=2)
                                                                                                                       cTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAg                                              <  1:202110/110‑1 (MQ=2)
                                                                                                                       cTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCTGCGCAACGTTAATCGACGCAGGGTTAGTCggttc     >  2:301919/1‑147 (MQ=2)
                                                                                                                       cTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCTGCGCAACGTTAATCGACGCAGGGTTAGTCggttc     >  2:381171/1‑147 (MQ=2)
                                                                                                                       cTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCTGCGCAACGTTAATCGACGCAGGGTTAGTCggttc     >  1:59376/1‑147 (MQ=2)
                                                                                                                        tGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAg                                                    <  1:154769/103‑1 (MQ=2)
                                                                                                                        tGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAgg                                             >  2:184861/1‑110 (MQ=2)
                                                                                                                        tGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAgg                                             <  1:184829/110‑1 (MQ=2)
                                                                                                                        tGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCTgc                                     <  1:149151/118‑2 (MQ=2)
                                                                                                                        tGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCTgc                                     >  2:149180/1‑117 (MQ=2)
                                                                                                                         gTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCt   >  2:19039/1‑151 (MQ=31)
                                                                                                                         gTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCTGCGCAACGTTAATCGACGCAGGGTTAGTCggtg      <  2:330913/148‑4 (MQ=2)
                                                                                                                           aaGCTTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTa  >  2:635946/1‑150 (MQ=14)
                                                                                                                            aGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCaa                                               >  2:679818/1‑104 (MQ=2)
                                                                                                                            aGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAgg                                             >  2:245096/1‑106 (MQ=2)
                                                                                                                            aGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAgg                                             <  1:245051/106‑1 (MQ=2)
                                                                                                                             gCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAg                                              >  1:74022/1‑104 (MQ=2)
                                                                                                                             gCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAg                                              <  2:74039/104‑1 (MQ=2)
                                                                                                                               cTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAgg                                             >  2:282830/1‑103 (MQ=2)
                                                                                                                                 gtgAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGGGTGTGAAAAACACCCACGCCGAAAGACCAAGGGTTCCTgcg                                    >  1:6695/1‑108 (MQ=2)
                                                                                                                                                  |                                                                                                                              
TTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTA  >  NC_000913/4038599‑4038870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑