Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,203	C→T	W36* (TGG→TGA)	rob ←	right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,203	0	C	T	100.0%	253.9 / NA	67	W36* (TGG→TGA)	rob	right oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/25);  total (42/25)

TTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGAC  >  NC_000913/4635056‑4635346                                                                                                                                                    |                                                                                                                                                ttGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCAc                                                                                                                                              >  2:642699/1‑151 (MQ=255) ttGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCAc                                                                                                                                              >  1:372694/1‑151 (MQ=255)    agagTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACt                                                                                                                                             <  2:758963/149‑1 (MQ=255)      agTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTgg                                                                                                                                          >  2:822776/1‑150 (MQ=255)       gTCGAGGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGGCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGa                                                                                                                                         <  1:552773/150‑1 (MQ=255)       gTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGa                                                                                                                                         >  2:608693/1‑150 (MQ=255)        tCGAGGCGGTATTGCAGCGGGATGTCCAGAATCGGCCGCGCAGTCGGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGa                                                                                                                                         <  1:773652/149‑1 (MQ=255)        tCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAAt                                                                                                                                       >  2:755264/1‑151 (MQ=255)          gAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATa                                                                                                                                      >  2:77699/1‑150 (MQ=255)          gAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATa                                                                                                                                      >  1:566831/1‑150 (MQ=255)              cGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTg                                                                                                                                 >  2:355032/1‑151 (MQ=255)               ggTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGc                                                                                                                                >  1:327289/1‑151 (MQ=255)               ggTATGGCGGCGCGATGCCCAGAAGCGGCCGCGCAGGCGGGCGTGGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTg                                                                                                                                 <  2:164499/150‑1 (MQ=255)                gTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCt                                                                                                                               >  1:267900/1‑151 (MQ=255)                   ttGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTc                                                                                                                            >  2:733110/1‑151 (MQ=255)                    tGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTcg                                                                                                                           >  1:822536/1‑151 (MQ=255)                    tGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTcg                                                                                                                           >  2:405103/1‑151 (MQ=255)                       aGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTcgccg                                                                                                                        >  2:146675/1‑151 (MQ=255)                        gcgcGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTcgcc                                                                                                                         <  2:695964/149‑1 (MQ=255)                          gcgATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCt                                                                                                                      >  1:681250/1‑150 (MQ=255)                          gcgATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCt                                                                                                                      >  1:718580/1‑150 (MQ=255)                          gcgATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCt                                                                                                                      >  2:71134/1‑150 (MQ=255)                             aTGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACAt                                                                                                                  >  2:314194/1‑151 (MQ=255)                                 caaGAATCGGACGCGCAGTCGGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTc                                                                                                              <  2:260785/149‑1 (MQ=255)                                  cAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCg                                                                                                             >  1:349540/1‑151 (MQ=255)                                     aaTCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAg                                                                                                           <  2:161244/150‑1 (MQ=255)                                                 agacAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTg                                                                                               >  1:50382/4‑150 (MQ=255)                                                  gTCAGGCGTAGTGCGCCCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGAt                                                                                             <  2:372761/151‑1 (MQ=255)                                                         gTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATg                                                                                      >  1:85865/1‑151 (MQ=255)                                                         gTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATg                                                                                      >  2:616815/1‑151 (MQ=255)                                                                  ccgccgATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTccag                                                                             <  1:514656/151‑1 (MQ=255)                                                                  ccgccgATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTcca                                                                              <  2:493217/150‑1 (MQ=255)                                                                  ccgccgATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTcca                                                                              <  1:620075/150‑1 (MQ=255)                                                                       gATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTccagcc                                                                           >  2:514269/1‑148 (MQ=255)                                                                              aCAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTaaa                                                                   >  2:441819/1‑149 (MQ=255)                                                                                aaaCGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAgg                                                                <  1:473560/150‑1 (MQ=255)                                                                                aaaCGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGt                                                               <  1:486122/151‑1 (MQ=255)                                                                                 aaCGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGt                                                               >  2:98146/1‑150 (MQ=255)                                                                                  acgacgAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcg                                                             <  2:540111/151‑1 (MQ=255)                                                                                   cgacgaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgc                                                            >  2:633475/1‑151 (MQ=255)                                                                                     acgaGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTcgcg                                                           >  2:128658/1‑150 (MQ=255)                                                                                         gCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGaata                                                       >  1:453417/1‑150 (MQ=255)                                                                                             gAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGcc                                                  >  2:809751/1‑151 (MQ=255)                                                                                              aaTATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCg                                                 >  1:474724/1‑151 (MQ=255)                                                                                                tataCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGc                                               >  2:46385/1‑151 (MQ=255)                                                                                                tataCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGc                                               >  1:403313/1‑151 (MQ=255)                                                                                                 ataCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGcc                                              <  2:674204/151‑1 (MQ=255)                                                                                                  taCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCt                                             >  2:419898/1‑151 (MQ=255)                                                                                                  taCGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCt                                             >  1:371239/1‑151 (MQ=255)                                                                                                     gcgcCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGAt                                          >  2:39632/1‑151 (MQ=255)                                                                                                     gcgcCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGAt                                          <  1:544569/151‑1 (MQ=255)                                                                                                             aGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATaaaa                                  <  1:405032/151‑1 (MQ=255)                                                                                                             aGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATaaa                                   <  2:280039/150‑1 (MQ=255)                                                                                                                aTGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAatat                               <  1:128944/151‑1 (MQ=255)                                                                                                                    ccAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTc                           >  2:735648/1‑151 (MQ=255)                                                                                                                    ccAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTc                           >  1:596429/1‑151 (MQ=255)                                                                                                                     cAGTGACATCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCa                          <  2:276388/151‑1 (MQ=255)                                                                                                                            aTCTTTAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTc                   >  2:803465/1‑151 (MQ=255)                                                                                                                                 tAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTcaaca               <  2:566929/150‑1 (MQ=255)                                                                                                                                 tAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTcaaca               >  2:469180/1‑150 (MQ=255)                                                                                                                                 tAAACATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTcaaca               >  2:202658/1‑150 (MQ=255)                                                                                                                                  aaaCATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCaacaac             <  2:681368/151‑1 (MQ=255)                                                                                                                                  aaaCATTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCaacaac             <  2:349603/151‑1 (MQ=255)                                                                                                                                      aTTCTCTGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGc         <  1:128630/151‑1 (MQ=255)                                                                                                                                          tctGTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCt     >  1:326229/1‑151 (MQ=255)                                                                                                                                             gTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCt     <  2:718705/148‑1 (MQ=255)                                                                                                                                             gTAAGTGTCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGAc  >  2:717954/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                TTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACCGCCGATTTCGACAAACGACGAGCACGAATATACGCGCCAATAGCATGGCCAGTGACATCTTTAAACATTCTCTGTAAGTGCCACTTGGAATAACCTGCTTTCGCCGCTACATTGTCGAGCGACAGGGGCTGATCCAGATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGAC  >  NC_000913/4635056‑4635346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑